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A 260 /A 280
All kits were also ranked from 1 (lowest) to 5 (highest) for 2 1.0
3 1.5
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Results
Mini column–based2 PCR cleanup 1.0
The GeneJET PCR Purification Kit, which contains silica
mini spin columns, can be used for purification of DNA
1 0.5
fragments from 25 bp to 20 kb with 90–100% recovery.
The GeneJET purification columns have a high binding Figure 1. Mini column–based PCR cleanup. (A) DNA yield (blue) and
capacity of up to025 µg
364
of DNA.
1,136
The
364
entire purification
1,136 364 1,136
0 purity (green), and (B) gel analysis of PCR products using 3 different PCR
cleanup kits. For gel analysis, 200 ng of input amount was used for 364
procedure takes onlyGeneJET
about kit
5 minutes forkit
QIAquick a single sample.
NucleoSpin kit
bp and 1,136 bp DNA. The Invitrogen™ E-Gel™ 1 Kb Plus DNA Ladder was
used as the size standard.
for all three kits. The GeneJET kit has the fastest protocol,
A 260 /A 280
2.08
1.0 2.06
with fewest steps, as well as the lowest price per prep.
2.04
0.5
2.02
Micro column–based PCR cleanup 0 2.00
364 1,136 364 1,136
The PureLink PCR Micro Kit, which contains silica micro PureLink kit MinElute kit
spin columns, is ideal for purifying and concentrating DNA
2.5 2.16
from low-yielding PCR or restriction digestion reactions. B Original PureLink Kit MinElute kit
2.14 364 1,136 1,136 364 1,136 364
The kit provides
2.0 a simple and rapid method for purifying
2.12
Average yield (µg)
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2.08
up to 95% recovery of DNA fragments with as little as 5 µL
1.0 2.06
of elution volume. Using this kit, the efficiency of removing
2.04
primers, dNTPs,
0.5 enzymes, and salts exceeds 98.5%.
The PureLink purification micro columns have a binding 2.02
capacity of up0 to 5 µg
364
of double-stranded
1,136
DNA.
364
The 1,136 2.00
2.0
1.5
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1.5
1.0
1.0
0.5
0.5
0 0
364 1,136 364 1,136 364 1,136
MagAttract kit ChargeSwitch kit AMPure kit
1.5
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1.0
0.5
Figure 3. Magnetic bead–based PCR cleanup. (A) DNA yield (blue)
and purity (green), and (B) gel analysis of PCR products using 3 different
0
,136 PCR cleanup kits. For gel analysis, 200 ng of input amount was used for
t 364 bp and 1,136 bp DNA. The E-Gel 1 Kb Plus DNA Ladder was used as
the size standard.