Energy +P—z>M} 0 BOS PM cn (Gb) 54P—oM ‘The dry weight of the cel is Tand is equal to @ T=5,4P + M=—V where p = cell density and V= cell volume Write the equations and define all symbols necessary to describe the changes in SP, Mf, and within the cell. Remember that the cell volume is aways changing, 65. A biochemical engineer has determined inher lab that the optimal productivity of a valuable an- Uibioc is achieved when the carbon nutrient, im thie case molases, is metered into the fermenter at arte proportional to the growth rate, However, she cannot implement her discovery inthe a- Uibioc plan, since there is no reliable way to measure the growih rate (d/h) or biomass con- centration (X) during the course of the fermentation. It is suggested that an oxygen analyzer he installed on the plant fermenters so thatthe OUR (oxygen uptake rate, gh) may be measured, a. Derive expressions that may be used to estimate X and dX/dr from OUR and time data sssuming that a simple yield and maintenance model may be used to describe the rte of ‘oxygen consumption by the culture D. Calculate values for the yield (Yyp,) and maintenance (m,) parameters from the follow. ing data: ou x @n 060) 063 063 076 106 156 223 235 as 537 159 940 nao nm B00 Lar a7 1537 62 els 2 0861667 21090 ror [Couresy of D.Zabsiske rom “Collected Coursework Problems in Biochemical Rngncerng” com piled by H.W. Blanch for 1977 Am. Soc, Eng. Educ. Sumer Schoo] 202 How Cells Grow Chap. 6