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Effects of Methanol Ethanol and Aqueous Extract of
Effects of Methanol Ethanol and Aqueous Extract of
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Universiti Kebangsaan Malaysia
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Sharifa A.A., Neoh Y.L.., Iswadi M.I., Khairul O., Abdul Halim M., Jamaludin M., Mohamed
Azman A.B and H.L. Hing.
Department of Biomedical Sciences, Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, 50300
Kuala Lumpur, Malaysia.
ABSTRACT
Plantago major which is widely distributed in Malaysia was taken to study the antibacterial
effect against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Candida albicans and
Candida tropicalis. The whole plant was extracted using methanol, ethanol and aqueous as solvent
extractor. The results showed that methanol extracts of Plantago major have antibacterial effects
against Gram positive and Gram negative bacteria such as S. aureus with MIC value of 100mg/
ml and E. coli with MIC value of 120mg/ml. The ethanol extract of P. major also showed an
antibacterial effect on S. aureus with MIC value of 200mg/ml and on E. coli with MIC value
of 140mg/ml. There is no antibacterial effect of methanol and ethanol extracts of P. major on
B. subtilis. The aqueous extract of P. major has no antibacterial effect on S. aureus, E. coli and
B. subtilis. All these extracts with MIC value between 100–200mg/ml shows no effects on C.
albicans and C. Tropicalis at all.
INTRODUCTION
Whole Plantago major plants (stems, leaves and roots) were collected from Cameroon
Highland and identified. The fresh specimen was washed, cut into small pieces and dried at room
temperature for a week before Soxhlet extracted with methanol, ethanol and aqueous method
(Ibrahim et al. 1996). Extracts obtained were tested on S. aureus, B. subtilis, E. coli, C. Albicans,
and C. Tropicalis. Sensitivity test was done using disc diffusion with positive effects of extracts
samples observed with Scanning Electron Microscopy.
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ANNALS OF MICROSCOPY Vol 8, April 2008
Figure 1. Untreated S. aureus used as control Figure 2. S. aureus treated with methanol P. major extract
(100mg/ml).
Figure 3. S. aureus treated with ethanol of P. major Figure 4. Untreated E. coli used as control
extract (200mg/ml).
Figure 5. E. coli treated with methanol extract P. major Figure 6. E.coli treated with ethanol P. major extract
extract (120mg/ml). (140mg/ml).
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ANNALS OF MICROSCOPY Vol 8, April 2008
The antibacterial effect of various extracts of P. major were seen on S. aureus, and E. coli.
After exposure to 200 mg/ml of methanol and ethanol of P. major, the cell wall of S. aureus was
crenated and cells were sticky (Figure 2 & 3), almost seen like a shrunken cells as compared to
control (Figure 1). With E. coli, methanol and ethanol extracts of P. major showed blebs formation
and indentations (Figure 5 & 6) as compare to control (Figure 4). The aqueous extract has no effect
on the bacteria tested. This fact is similar to the findings of Hetland et al. (1999). The MIC value of
methanol extract was lower than ethanol showing that there was more active antibacterial ingredient
in methanol than in ethanol extract. The findings were similar to the findings of Samuelsen et al.
(2000) and Hoj et al. (2001). All the three extracts have no effects on the yeast tested.
CONCLUSIONS
ACKNOWLEDGEMENTS
We would like to acknowledge the support given by Faculty of Allied Health Sciences,
Universiti Kebangsaan Malaysia (UKM) and Forest Research Institute of Malaysia (FRIM).
REFERENCES
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