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The constant curiosity of humanity, whether to understand religious and cultural themes of its
past or even for academic purposes (Gaston 2018), has highlighted the importance of Archae-
ology and the archaeological research itself. What the meticulous and constant archaeological
research of past life has revealed, is that all discoveries matter. Objects of major or minor im-
portance have their value, as well as human osteological remains. Especially skeletal remains
constitute a substantial tool to perceive past human activity in all its various forms. From a
basic inventory, where researchers determine the number of individuals and the presence of
their bones, to various microscopic studies, ancient human remains can provide answers to a
multitude of questions.
Therefore, acquiring new scientific methods and designingauxiliarytools has become para-
mount for the research of osteological remains (Ubelaker 1989), as their study is steadily grow-
ing (Buikstra and Beck 2017). With the integration of Osteoarchaeology, researchers could
create a more concise picture of the past populations and their daily life. Data about their sex,
age, stature, and pathological conditions are requisite about the palaeodemographic study of a
population, the circumstances surrounding their death (White and Folkens 2005), along with
the socioeconomic conditions of the society itself (Follér 1992).
The origin, development, and progress of disease in past generations, as to how these indi-
viduals adapted to environmental changes can be investigated through the study of palaeopathol-
ogy (Nesse and Williams 1994; Aufderheide and Rodriguez – Martin 1998). Palaeopathology
can be considered as a sub-discipline of osteoarchaeology – biological anthropology, focusing
on primary evidence of abnormalities from human osteological remains that could indicate
the presence of a health affliction (Roberts and Manchester 2007).
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Even though in the past, understanding of pathologies was problematic, due to the failure
in interpreting the nature of the disease itself, significant improvements have been made in
medical diagnostic procedures over the last century (Metcalfe 2007). A palaepathologist of
modern days is able to investigate and diagnose traces of pathologies on human skeletal re-
mains regardless of their condition (complete or fragmented). With several techniques, which
have been applied to previous populations, palaeopathologists can formulate data and infor-
mation for a wide range of medical problems and indicate how these might have affected their
living conditions.
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Applied Techniques and Methodologies
Despite the problems that may arise from recording a collection of commingled and frag-
mented human remains, they are important for the osteoarchaeological research as they can
provide a wealth of data. The collected fragments that were initially recovered should be di-
vided preliminary between human and non – human, so to limit the possibility of including
elements from an animal, pottery, or rock (Osterholtz 2019).
A methodology that is used as a common next step involves sorting the cataloged sample
in similar elements (i.e. humeri, femora, etc.) and the side of the body they come from,
where the most represented element could assist in determining the MNI (Minimum Number
of Individuals) in the studied sample (Buikstraet al. 1994; Adams and Byrd 2006; Konigsberg
and Adams 2014). For example, six right tibiae would indicate the presence of six different
individuals. A researcher could enhance the accuracy of the method about MNI by applying
osteometrics on the long bones. Measurements could be taken for similarities in robusticity
and bilateral symmetry, as they can determine if any skeletal elements are a pair (Byrd 2008;
Nikita and Lahr 2011; Byrd and LeGarde 2014).
Another way to assess not only the minimum estate but the original number of individuals
from whom the skeletal elements come from is the MLNI (Most Likely Number of Individ-
uals) (Adamset al. 2008; Nikitaet al. 2019). In order to use this technique, two paired bones
should be analyzed whether they pair together as part of one individual. With the completion
of assessing pair matches, hypergeometric probability functions could be used to support with
a confidence interval that estimate.
The estimation of the biological profile of human skeletal remains, even from commin-
gled and fragmented, is crucial for the osteoarchaeological research. Thus, when the MNI
is known, every element that contains diagnostic features for sexing and aging should be
recorded. Those can include applicable traits from pubic and cranial bones for sex assess-
ment (Buikstra and Ubelaker 1994) and clear indication of juvenile attributes like epiphysial
fusion, presence of deciduous teeth or the alveolar, and the size of the long bones for age es-
timation (Rissech et al. 2003; Schaefer et al. 2009; Cardoso et al. 2017). In regards to the
stature estimation, commingled remains are often in fragmentary condition, which limits the
potential for accurate calculation. Besides their limitations, regression equations have been
proposed for fragmented remains for the height evaluation (Bidmos 2008).
As for the presence of pathological markers on the bones, each element should be assessed
for abnormalities and peculiarities that could suggest diagnostics of possible maladies. De-
spite the difficulty to estimate the actual prevalence of pathological conditions, since malfor-
mations and traits are identified on individual skeletal elements, palaeopathological analysis
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of commingled and fragmented human remains is still of great value and importance for the
reconstruction of the biological profile (Brickley and Buckberry 2015).
Except for the macroscopic methods, former microscopic techniques like using X-ray
fluorescence spectrometry have revealed promising results in an attempt to determine the
number of individuals that are associated with an elemental concentration of human remains
(Gonzalez-Rodriguez and Fowler 2013; Stevens 2016). It should be noted that this kind of
scientific technique could provide data without destroying the bones, compared to the macro-
scopic analysis.
Another microscopic approach that is being gradually employed, in cases with multiple
human skeletal remains, is DNA analysis. (Mundorff et al. 2014; Verdugo et al. 2017) How-
ever, because of its high cost for implementation and the need to destroy part or whole of
the bone, it could only provide information when used in conjunction with the macroscopic
osteological analysis (Puerto et al. 2014; Geigl and Grange 2018).
Conclusion
The palaeopathological examination of human skeletal remains has seen substantial growth
and has increased the knowledge of diseases occurring in various periods with the presence of
human activity. In some cases, it has been feasible to provide biological responses to questions
regarding traits of possible diseases, their social and economic transformation process, and
their transition through the age with data that were extracted from complete, disarticulated,
and commingled osteological remains.
The study of commingled human skeletal remains has been approached with less interest
in the past, because of the complexities and challenges facts that those samples pose to the
osteoarchaeologists. Nonetheless, their value to the archaeological research is of major impor-
tance as it may form a far more comprehensive understanding of past peoples’ life. With the
advancement of technology and the integration of alternative techniques in conjunction with
the macroscopic analysis, researchers have managed to yield fruitful results and add more to
the known information.
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