MENGHITUNG ASAM AMINO PADA PROTEIN PATATIN

Solanum tuberosum (Potato)

Mata Kuliah Analisa Pangan Terpadu

Disusun Oleh:
SITI KHOLIFAH
20.I3.0001

PROGRAM MAGISTER TEKNOLOGI PANGAN

UNIVERSITAS KATOLIK SOEGIJAPRANATA
2020
PAT0_SOLTU (P07745)
Patatin precursor (EC 3.1.1.-) (Potato tuber protein)
Solanum tuberosum (Potato)

The computation has been carried out on the complete sequence (386 amino acids).
Number of amino acids: 386
Molecular weight: 42490.27
Theoretical pI: 5.34

Amino acid composition :

Ala (A) 38 9.8%
Arg (R) 11 2.8%
Asn (N) 17 4.4%
Asp (D) 21 5.4%
Cys (C) 2 0.5%
Gln (Q) 14 3.6%
Glu (E) 22 5.7%
Gly (G) 23 6.0%
His (H) 8 2.1%
Ile (I) 21 5.4%
Leu (L) 40 10.4%
Lys (K) 22 5.7%
Met (M) 11 2.8%
Phe (F) 19 4.9%
Pro (P) 16 4.1%
Ser (S) 29 7.5%
Thr (T) 35 9.1%
Trp (W) 2 0.5%
Tyr (Y) 16 4.1%
Val (V) 19 4.9%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 43

Total number of positively charged residues (Arg + Lys): 33
Atomic composition:
Carbon C 1904
Hydrogen H 2974
Nitrogen N 490
Oxygen O 584
Sulfur S 13
Formula: C1904H2974N490O584S13
Total number of atoms: 5965

Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 34965
Abs 0.1% (=1 g/l) 0.823, assuming all pairs of Cys residues form cystines
Ext. coefficient 34840
Abs 0.1% (=1 g/l) 0.820, assuming all Cys residues are reduced

Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.49
This classifies the protein as stable.
Aliphatic index: 85.75
Grand average of hydropathicity (GRAVY): -0.133

Sumber :
https://web.expasy.org/cgi-bin/protparam/protparam1?P07745@noft@di akses pada Tanggal 23
Oktober 2020
https://www.uniprot.org/uniprot/P07745di akses pada Tanggal 23 Oktober 2020