N ‘AA === chssmate (7 page ——_ C= Pegorshian flit 008 based on —mnlotly, norenality geccentoge dilutrons be . — = fit To Sescrrbe the coneentratvans af solatvans quart tntnet _ Molarrte Melanie y CM) ig Be meant a su stance in _cortarin volume 9| sodutvon Moloatty 1 drned os moles qo soluke per Adees of a s0tdlior Molarity 15 algg known a3 molar concentraly'on gf a__sobub ton » : Number of wales ak cotle drssoled = w 4 WA yeml water have sw mole of solute M tooo ml water have WX1000 MxVG, my No. of milWenates = mass of solute x 1000 Mel. wt. af solede = Molorrky af soduty on x Vin me - Moloat= Ne mittrnsle of solube Total voearne of gotty 0 to ml Moalarity(M)= wx 009 mol. wit. of solube 9 Nin mifyseduc eT ae a Jo prepare 500 mi 005 soludg , j Con | ousnel Nacl- Stuy —* molecular _werght Nocl = 58. - Imre -_ £._ _~=~=~=—rs——<“(— CT -_=-_—s— ~ Tideborolory , obtain Nac] with an using balance and paper Measure ty nd 7. 304q Nacl. “ |- _ Record the exact mass and 4ans{er then nip I approperate sized _volumelarc (orks es Adel water into vodumelarc {lash alle win r the soduke to be dissolved Co dri fast ballon r | the_ fost) AY “= When NaCl 13 comp lekeley dusched gL wag LAL neck ofthe fleck carddatlg and T doug = When you reach the edge fine a te deal > drop to get Lower meniscus ase solvent solvent — direcly . fovcled with the 4ne He tie, E \-classmate Date____—— Page frecaut on |= Grack amount of solute should be calculated 40-7 | gormula and pub $n the Hook . Th excess nevey pub hack the eoceoss reagent into origrnal container. Al _ solute hos to be dissolved | in the flask - be sure to mix salubron _by inverting the volurmebric __fask several Limes before aging =the Lower mentsug vol s solvent should be tevcledd with the Zine of flask - = Adter preparakron , the content of the solubre should be leads @abelled on the container | fer Laboratory safety purpose + | | Problenn te - * ow wang grams of Nao ae needed fe _prepare | iL ok 1M Naot ol? 2 = Notas Mobes ob salute el a 5012 (LI iM= ao _ ee = 7 az fb mol : ee 4 jo] Wao 40g _ | : ————— od spare iL 1M Woop! 40g of Know te wed fo pref % so! Q| What rs melartty al a selutren i 7 6.154 Nacl deeowed hi wader 4 magy Or wv a wth «total volume of 452 ml- Sty. 4 ~~ Molarity= 6.459 x 1000 = 0.256 mol [y i 45 Omh_x 56. 4glenol ~ ™ 596.8 [= Motaitt Motatrey 18 an odditveral way to tcorg the strength or _concentradion 4 soludion. ~ m= moles of solide hg of solvent oo! foblero Coleudate molality of sol” _contarnn'ng 9125 mol a non-eedrotyte solute dissolved in OOH stlvent» me alesmél = o.5moallkc 2. kg‘ kg a solvent Sky classmate th —= Og a= Al 254 Natl is dissolved i soooml water » find molalik) | ag esulbong sei? P| | 85g oh Nach = Imol of Nat! Pal 254 = 1x25 = 0.429 mol _Na Cl ol 58.5 ——| gince denstky of water 18 lglmb- — | go00 ml = 5000 q= 5kg - 4 —~ | n= mol of solibe = 0.42¢ mol 2 0.0854 mall ky || a 4 [Relation bekweer _mobarity & molatt : —<—$—— ‘ ia, Ni | Molar toc M_of goludi0n means M 1 me go | are eresent tm 000 em of sa 8udy ok TE deorsity of so 15 § gler? - then, mass al sof? = tooo d gram: | moss af soute= MM, (My = mel. wa wood - Mg St Ls mass of solvent = | Mobaity Cm) — a iced wood - MMp r Mie ond ltmMy] 1009N \ ; \ \ \\\) \\ Norma ety Wssolved iy iL dt — sofukyon TL_1 number eh gram equivalent of solu @ N= gro 9g. of solute Mel of sol? in Lilre = weight x 1900 Gurvaleol weight me. equivalent weight = _mélar_maos 7 Normahka represents molar concentration onky of ccid_companent_ cyt dor ocrd ) or onky for base componed CoH” tor hase) . N= Mx No. a Hor oH Hons - WV Prableno Fin nermatity of 2604 bowing “J g of MSO prov ent in 500 ml of sol?. _emng5_ soluke x 1000 eprvetent mass x Val(in ml) Nee Miaileod — oN. 38 x500ssiireaseeey gee eae eee c : Pa ay. Cmly $0 canbe Ni ql What vel: Fhe duking 25h of M(H sol? 2 i Gye te (mlv) N= 25 ml | Coz 2% (mlV) Ne | Cu = CoNe Vg = CMs Co T |! = 86 IY | 2 T ! = 185mlSerta diludron Aim: 40 estimate —cénconbyabran (no. 6h colon'es Sryantions backerta, Viruses ) of unknown somp/ by counting number of cdlontes — calfered $09 Servol dilutvons of sorople ard back frock measured 7 aunts tg unknawn concentration . = ‘Theory 2 Procedure . A cenal dilechon 18 a _ simple fechnqae for reducing _ concentracy on 4 a sf im systemabc weg: 4 7 Indore -deperdent ctedy , stark by “ang _ ime | stock concentration chemical, tansterti99, it into del of water ond mrsding . Use thes constant — _diluvon fackor though out the eres ty _prode, 0 expe mental concentration 1M, aM , 0-01M, 0.001 M f