Ethanolic extract of Yellow bell (Tecoma stans) leaves as agent in killing

Aspergillus niger

Rationale: Tecoma stans also know as yellow bell, yellow elder, trumpet flower in
English and Piliya in hindi. Tecoma stans is an important medicinal plant. The leaves,
bark and roots contains biologically active chemicals, and extracts from those tissues are
in use as traditional folk medicines. Tecoma stans is a herbal medicine used for treatment
of diabetes, digestive problems, control of yeast infections, as powerful diuretic,
vermifuge and tonic. Preliminary phytochemical screening of this plant revealed the
presence of tannins, flavonoids, alkaloids, quinones and traces of saponins and amino
acids.

In bioassay-guided fractionation of the leaves of the Tecoma stans

dichloromethane (DCM) extract contained one major compound that was isolated and
characterised as oleanolic acid. The DCM extract and oleanolic acid were active plant
fungal pathogens.

Aspergillus niger is a fungus and one of the most common species of the genus
Aspergillus. It causes a disease called "black mold" on certain fruits and vegetables such
as grapes, apricots, onions, and peanuts, and is a common contaminant of food.

Research Question:

1. Is the Yellow bell (Tecoma stans) is effective in killing aspergillus niger?

2. If the researcher use Tecoma stans in killing Aspergillus niger it will totally kill as
100%?

Hypothesis
Null
1. Yellow bell (Tecoma stans) is not effective in killing Aspergillus Niger.
2. If the researcher use Tecoma stans the Aspegillus niger will not kill the
bacteria.

Alternative

1. Yellow bell (Tecoma stans) is effective in killing Aspergillus niger.

2. If the researcher will use the Tecoma stans the Apergillus niger will kill the
bacteria 100%.
Procedure:
In making or in killing Aspergillus niger, The researcher will gather the
plant after that the researcher will extract and soak the plant in the school
laboratory. The researcher will go to the nearest laboratory to have the
phytochemical analysis to know the component of the plant. The researcher
will get the extract and the researcher will get a specific substance and the
extract of the yellow bell (tecoma stans) will be tested to the onion and the
researcher will get the result and identify if the Yellow bell (Tecoma stans) is
effective in killing an Aspergillus niger.

Risk and safety:

Take care the laboratory equipment because it may get broken. Wear
always an laboratory gown and mass if you will get in the laboratory.

Data Analysis:
The researcher will analyse the data and results if its effective or not.

Bibliography: