GUT MICROBIOME AND ASSOCIATION WITH DISEASE

Dissertation

Submitted in Partial Fulfillment for the Degree of Master of Science in Biochemistry

To

RTM NAGPUR UNIVERSITY, NAGPUR

By

Miss. Gayatri R. Hatwar

(M.Sc. Biochemistry, Semester IV)

Session 2020-21

Guided By

Dr. Mashitha Pise

HoD, Department of Biochemistry
Hislop College, Nagpur

HISLOP COLLEGE, TEMPLE ROAD, CIVIL LINES, NAGPUR- 440001

2020-21
 CERTIFICATE

This is to certify that the dissertation work entitled “Gut Microbiome and Association with

Disease” has been carried out by Miss. Gayatri Ravindra Hatwar, student of M.Sc.

Biochemistry (Semester IV), session 2020-21, of Hislop College, Civil lines, Nagpur, in the

partial fulfillment of the requirement for the award of Master’s degree in Science in the subject

of Biochemistry as prescribed by the Rashtrasant Tukadoji Maharaj Nagpur University.

Dr. Prashant Shelke

Officiating Principal,

Hislop College, Nagpur

 CERTIFICATE

This is to certify that the investigations described in this thesis entitled “Gut Microbiome and

Association with Disease” was carried out by Miss. Gayatri Ravindra Hatwar in the

Department of Biochemistry, Hislop college, Nagpur, under my supervision.

Dr. Mashitha Pise

HoD, Department of Biochemistry
Hislop College, Nagpur
 DECLARATION

I, Miss. Gayatri Ravindra Hatwar, student of M.Sc. Biochemistry Semester IV, Hislop

College, Nagpur declare that the dissertation work entitled “Gut Microbiome and Association

with Disease” is based on the findings of original research work carried out by me at Department

of Biochemistry, Hislop College, under the supervision of Dr. Mashitha Pise. No part of this

research has been published or submitted for the award of any diploma or degree in any

University/ Institute.

DATE:

PLACE: Nagpur Miss. Gayatri Hatwar

 ACKNOWLEDGEMENT

I would like to thank the God almighty who gave his silent showers of blessing for entire good

things in my life. It is good for me to have any opportunity to acknowledge feelings of deep

gratitude and indebtedness to all those, who direct or indirect way help me in carrying out this

dissertation successfully.

My first and foremost thanks to Dr. Prashant Shelke, Principal, Hislop College, Nagpur who

gave me this wonderful opportunity to work in Hislop College, Nagpur.

My deep sense of gratitude toward my guide Dr. (Mrs.) Mashitha Pise, Head of Department of

Biochemistry, Hislop College, Nagpur, for her valuable guidance, imparting knowledge and

critical analysis of my result, which led to successful completion of this dissertation. Her

scientific approach, promptness to deal with the matter in time and thoroughness taught me

lesson for my future.

I am extremely thankful to Dr. (Mrs.) Mashitha Pise Nagpur, who despite having busy schedule,

was always ready to help me with her excellent ideas and her immense help, deep support,

guidance and encouragement.

I am thankful to my teacher, Dr. (Mrs.) Rashmi Padhye, Mrs. Sudipta Sarkar, Mrs Richa Dubey

for their help, support and encouragement.

I am thankful to Mr. Ritesh Sarjare, Library incharge of the Department for their support,

suggestion.

My heartiest thanks to my mother Mrs. Ranjana Hatwar and my sister Rashmi Hatwar and my

dear friends Mansi, Neha and Aiman and Nikita for their constant support, understanding and

timely cooperation. Without their support this couldn’t have been possible.
I am very much thankful to Mr. Mahadeo Kamatkar for skilled typing of the manuscript within

time.

I am very thankful to all my classmates Afreen, Aiman, Bhagyashree, Chetana, Daniel,

Jemaimah, Mansi, Nikita, Neha, Manisha, Pallavi, Riya, Rachana, Shibba, Sanskriti, Treta and

Vinay for their affection, moral support and suggestions.

Date: Gayatri Hatwar,

Place: Nagpur M.Sc. Biochemistry

Semester IV,

Hislop College, Nagpur

 CONTENTS

PARTICULARS PAGE

NO.

List of Tables I

List of Figures II

Abbreviations used III

Abstract 1

Introduction 2

1. What is meant by Gut microbiome? 3

2. How do we get Gut Microbiota? 4

3. What is meant by healthy gut microbiome? 6

4. Relation to Disease 12

5. Dysbiosis 14

A. Association with Intestine 16-23

i) Inflammatory Bowels Disease 16

ii) Irritable Bowels Syndrome 20

B. Association with Heart 23-26

i) Heart Failure 25

C. Association with Metabolic Syndromes 26-35

i) Diabetes 26

ii) Obesity 30

iii) Hypertension 32

D. Association with Kidney 35

 E. Association with Liver 38

F. Association with Brain 41-45

i) Autism 44

G. Association with Cancer 46-50

i) Gastric cancer 47

ii) Colorectal Cancer 48

iii) Oesophageal Cancer 49

H. Association with Allergic Conditions 50

i) Atopic eczema 52

ii) Asthma 52

6. Role of Diet 54

7. Therapeutic Intervention 57-64

i) Prebiotics 57

ii) Antibiotics 59

iii) Prebiotics 60

iv) Fecal Microbiome Transfer (FMT) 62

Future Perspective 65-66

References 67-75