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Agilent 5977B GC-MSD System
Agilent 5977B GC-MSD System
This powerful GC/MSD builds on a long track record of innovation, bringing together the
technologies of one of the industry’s best GC and MS systems. Agilent has pushed the
technological frontier even further by introducing the high-efficiency source (HES).
What’s more, the system’s proven robustness and reliability mean less maintenance and more
uninterrupted productivity. Simply put, the 5977B GC/MSD can help you meet your toughest
challenges and your most ambitious business goals… today and in the future.
2
x102 + EIC (202.20)
1 Analytical Challenge Agilent 5977B HES System Benefits
0.9
0.8 2 pg pyrene (EIC) – Design innovations increase performance for high analytical
0.7 n=8 sensitivity and simpler workflows
More to do, fewer people to do it
0.6 MDL=0.24 pg – Less maintenance and increased instrument uptime
0.5 (%RSD 3.94)
0.4 Superior analytical
– 10 times greater analytical sensitivity
0.3 sensitivity in Demanding measurement challenges
full-scan mode – IDL as low as 1.5 fg
0.2
0.1
0 – 10 times less sample required
– Spend less time on sample preparation and maintenance
12.6512.7 12.75 12.8 12.85
Counts (%) vs. acquisition time (min) Lab efficiency and profitability – Save on shipping costs
– Consistent, reliable performance lets you analyze large
Take advantage of the improved analytical sample batches over longer periods of time
sensitivity and see instrument detection
limits as low as 1.5 fg IDL. The 5977B
GC/MSD brings yesterday’s triple quadrupole
limits of detection into today’s single
quadrupole laboratory.
Agilent 5977B
Agilent 5977B EI MSD Agilent 5977B EI/CI MSD Agilent 5977B HES MSD
Inert Plus EI MSD
Analytical and The traditional stainless The inert extractor EI An inert extractor EI source The inert HES delivers
business solution steel source performs source enables high provides high analytical unparalleled analytical
most similar to previous analytical sensitivity for sensitivity. The CI source sensitivity for ultratrace-level
Agilent MSD instruments active compounds that are delivers softer ionization applications. Ideal for high-
at a budget-friendly price. most likely to interact with for molecular formula throughput labs that need
Designed for sample- noninert surfaces. Designed determination and enhanced to save time and money
dependent applications. for routine labs across selectivity. with extreme operational
various industry applications efficiencies.
for optimum operational
efficiency.
3
Innovative Technologies for
High Operational Efficiency
Boost your lab’s operational capabilities and achieve your business goals
High-efficiency source
This state-of-the-art ion source incorporates a novel design not seen in previous
EI source generations. It increases ionization efficiency and maximizes the
ions transferred into the quadrupole analyzer—so you can significantly improve
operational efficiency and analytical performance.
4
Clean, quiet, reliable, oil-free
5
A Faster Route to Insight
6
OpenLab CDS is best for quality control
testing in the pharmaceutical, chemical,
and energy markets.
7
Reliable and Unparalleled GC Separation with
Gold‑Standard Performance
Building the world’s most trusted GC system is an ongoing process. With every step
we increase speed, improve functionality, and incorporate new analytical capabilities,
while never losing sight of the most important objective—business results.
8
Unlock your lab's revenue potential
The Agilent Intuvo 9000 GC stands in a class by itself. The innovative
compact design offers fast direct heating, ferrule-free fittings, Guard Chip
technology, and no-trim columns to provide faster sample runs as well
as fewer and faster column changes. Intuvo continues the Agilent legacy
of reliability and gold-standard performance in a compact and powerful
complete package.
9
Lower Detection Limits and
Streamlined Workflows for
Food and Environmental Testing
A
75 TIC
233
50
25
0
23.98 24.05 24.12 24.19 24.26 24.33 24.40 24.47 24.54 24.61 24.67 24.74 24.81 24.88 24.95 25.02 25.09 25.16 25.23 25.30 25.37
Time (min)
60
6.5
55
B Scan 4026 (24.621 min)
Abundance [4943]
4.9
69
3.3 81 91 207
130
105 233
1.6 143 281
155 171 191 253 341
267 327
221 300 315 355 377388 405 429 449462 477 491
0
50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475
Mass-to-charge (m/z)
233
100 C Extracted spectrum (24.619 min)
75
Abundance
50
206
25
47 152 315
220 246 300 348 387 462
0
50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475
Mass-to-charge (m/z)
233
100 D RTL (MF = 84): Flusilazole
Analysis of 10 pg flusilazole in tomato using AMDIS.
75
A) Overlay of extracted ion m/z 233 (red) and TIC (black);
Abundance
50 B) Raw spectrum;
206
C) Extracted spectrum for the component;
25
47 123 139 151 165 183 220 246 260 300
315 D) Library spectrum, AMDIS match factor = 84.
63 77 91 109
0
50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 The reported NIST reverse match score is 73.
Mass-to-charge (m/z)
10
EPA 8270 re-optimized for the widest calibration range
EPA Method 8270 requires simultaneous measurement of diverse compound classes over a wide concentration range.
The 5977A and B Inert Plus GC/MSD meet these requirements over a range of 0.2 to 160 ppm using a single injection (one
calibration). Better initial calibration results lead to longer continuing calibration. Therefore, more samples may be analyzed
without operator intervention—lowering your cost of operation.
Here, we achieved a wider calibration range and low compound %RSDs, translating into greater laboratory productivity.
+ EIC (252.1)
Improved volatiles analysis using static headspace and the Agilent 5977B HES GC/MSD
The revolutionary design of the HES produces a higher ion current yield for many compounds. This greater analytical
sensitivity gives you the flexibility to lower detection limits, reduce sample size, and speed up your analysis.
We analyzed a mixture of volatile organic aromatic (VOA) compounds spiked into reverse osmosis (RO) water over a
calibration range of 0.02 to 20 μg/L. The analysis was performed in selected ion monitoring mode. Replicate injections
were made at 0.04 μg/L to assess the method detection limits (MDLs). A study of local tap water replicates was used
to demonstrate long-term stability for some naturally occurring compounds. The results suggest that a significant
improvement in detection limits is possible in VOA applications using the HES of the 5977B GC/MSD.
3.0
2.9
The Agilent SureTarget GC/MS Water Pollutants 2.8
2.3
2.2
pollutants, with minimal data processing time and fast 2.1
2.0
report generation. 1.9
1.8
1.7
1.6
1.5
4.76 4.78 4.80 4.82 4.84 4.86 4.88 4.90 4.92 4.94 4.96 4.98 5.00 5.02 5.04 5.06 5.08 5.10 5.12
Acquisition time (min)
11
Perform Reliable Chemical,
Petrochemical, and Materials
Identification and Quantitation
Phthalate plasticizers
Positive chemical ionization (PCI) lets you unambiguously identify different phthalates based on
an intense molecular ion response. You can configure the 5977B GC/MSD hardware to handle CI
reagents, including hydrocarbons (such as isobutane and methane) and softer reagents (such as
CO2 and NH3)—all with trace-level analytical sensitivity.
Biofuel characterization
The 5977B GC/MSD exceeds the criteria for accurate, sensitive biofuel analysis. Its inert flow path,
high-efficiency source, and heated gold-plated quartz quadrupole combine to deliver robust, high-
analytical sensitivity analysis for the full range of biofuel analytes.
The system is easily set up for simultaneous SIM/SCAN data acquisition to maximize analytical
sensitivity and selectivity, while providing full spectra for qualitative analysis.
12
387 fg TIC: CP120_150uLH2_175mL_5ppb_longtime_1.D\data.ms
(3.2 ppb) TIC: CP120_150uLH2_175mL_5ppb_longtime_100.D\data.ms (*)
TIC: CP120_150uLH2_175mL_5ppb_longtime_150.D\data.ms (*)
AsH 3 TIC: CP120_150uLH2_175mL_5ppb_longtime_200.D\data.ms (*)
TIC: CP120_150uLH2_175mL_5ppb_longtime_250.D\data.ms (*)
15,800 TIC: CP120_150uLH2_175mL_5ppb_longtime_300.D\data.ms (*)
TIC: CP120_150uLH2_175mL_5ppb_longtime_50.D\data.ms (*)
15,700
15,600
15,500
246 fg 322 fg
15,400 (3.4 ppb)
Abundance
(4.6 ppb)
H 2S COS
15,300 299 fg
(5.6 ppb)
15,200
PH 3
15,100
15,000
14,900
14,800
15.5 16.0 16.5 17.0 17.5 18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5
Time (min)
Overlay of total ion chromatograms (TICs) for every 50th run out of 300 runs completed over 4.5 days with ~5 ppb concentration analytes
in helium. Runs 1, 50, 100, 150, 200, 250, and 300 are shown.
13
Improve Your Compliance-Based
Pharmaceutical Residual Solvent
Analysis
Chromatograms
x107
3.8
3.6
3.4
3.2
3
2.8
2.6
Response [counts]
2.4
2.2
2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
1
2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10 10.5 11 11.5 12 12.5 13 13.5 14 14.5 15 15.5 16 16.5 17 17.5 18
Retention time [min]
Reference chromatogram for Class 2 (Mix A) residual solvents. Solvents were collected using the Agilent 7697A headspace
sampler and the 5977B GC/MSD on OpenLab CDS. The software gives you multiple ways to interact and present your GC/MS data,
while ensuring the high degree of data integrity expected in FDA regulated labs.
14
Quantify Drugs and Metabolites
More Quickly and Reliably
A
TIC
Hydrocodone (area)
= 1.6%
Acetaminophen (area)
109
B
m/z 151 EIC: acetaminophen
Peak area = 3,616,934
151
m/z
C 299
m/z 299 EIC: hydrocodone
Peak area = 57,225 Pulverized Vicodin tablet (5:300 mg of
242 hydrocodone:acetaminophen) analysis in ~one
minute. A) Total ion chromatogram (TIC). Extracted
96 185
ion chromatograms (EIC) for acetaminophen m/z
151 (B) and hydrocodone m/z 299 (C). NIST library
m/z
match >90 for both components.
0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Acquisition time (min)
glucose1
10
citrate
1
205 319
glucose2
urea
Metabolite identification in human blood plasma by GC/MS after methoximation and trimethylsilylation. The Agilent Fiehn library was also
used. Left panel: Total ion chromatogram, split 1:10 injection. Right panel: Identification of glucose using the NIST MS search and retention
time information.
16
Focus on System Validation
and Data Generation
Not Method Development
Agilent GC/MS analyzers are factory configured and chemically tested to meet method requirements
for materials, food safety, environmental, and forensic toxicology testing applications. These workflow
solutions get you on the fast track to producing quality data and processing sample backlogs.
Each analyzer arrives ready to perform with preset chromatography and checkout samples to verify
separation capabilities. That means your team can work toward system validation immediately after
installation, and reduce method development costs by up to 80%. As always, our support team is
available, should any problems arise.
17
Ensuring an Inert Flow Path Has
Never Been More Critical
As samples become smaller, increasingly active, and more complex, you cannot afford losses
caused by flow path activity. Having to repeat or verify suspect analyses wastes valuable resources,
hinders productivity, and hurts your bottom line. With trace amounts of active analytes, you might
not even get a second chance, because there may be no more sample left to analyze.
Stationary Data
Septum Fused silica phase
surface
Deactivation
Agilent Inert
Flow Path Ultra Inert
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
-Si-O-H
Ion
split/splitless GC liner source
inlet Inside GC column
Glass wool
Polyimide outer coating GC/MSD (or other detector)
Retention gap
18
An integrated approach to inertness: the Agilent advantage
As a GC industry leader, Agilent can help ensure the inertness of every surface that touches your
sample. So you can achieve the parts-per-billion, or parts-per-trillion, detection levels that today’s
analyses demand.
– Agilent J&W Ultra Inert GC columns are tested with the industry’s toughest test probe
mixture to ensure consistent column inertness and exceptionally low column bleed.
– U
ltra Inert inlet liners deliver a robust, reproducible, and reliable inert flow path, with or
without glass wool.
– I nert flow path split/splitless inlet option provides an extra measure of inertness to the
sample pathway.
– U
ltra Inert gold seals feature deactivation chemistry applied on top of their gold plating
for the most inert surface and highest-quality seal.
– U
ltiMetal Plus Flexible Metal ferrules are compatible with capillary flow technology
fittings. They promote a leak-free seal that requires less torque and reduces the risk
of column breakage.
– G
as Clean filter systems deliver the cleanest possible gas, reducing column damage,
analytical sensitivity loss, and downtime.
For more information about creating an inert GC flow path, visit www.agilent.com/chem/inert
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Agilent CrossLab services
CrossLab is an Agilent capability that integrates services and consumables to
support workflow success and important outcomes like improved productivity
and operational efficiency. Through CrossLab, Agilent strives to provide insight
in every interaction to help you achieve your goals. CrossLab offers method
optimization, flexible service plans, and training for all skill levels. We have many
other products and services to help you manage your instruments and your lab
for best performance.
Learn more about Agilent CrossLab, and see examples of insight that leads to
great outcomes, at www.agilent.com/crosslab
Learn more:
www.agilent.com/chem/5977b
Buy online:
www.agilent.com/chem/store
Europe:
info_agilent@agilent.com
Asia Pacific:
inquiry_lsca@agilent.com
DE.427986111