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EFL A 20041 22 AM ( SHIRE {Gd tetramethylbenzidine RG) + SME 205} Si © 10.45 FL A SOpt 2 1M BABE © 11, Bi BI LA BE FE 8 33 F ( ATC 400, SLT Labinstruments ) #1&ft > #52 i {13.450nm © TAS PASE ZS FH FZ RIE ( optical density, OD ) (845 A IL-6 HEHE wi BSR SK + ANAT Be BEAU E © COIL-6 EAE te 3 HT ASS AR AR IL-6 GIES AT > 79 FRE IL-6-dependent hybridoma cell line 7TD; (ATCC: CRL-1851 ) ZIRE aea AE 2) © MRIS RAD HERA MTT (3- ( 4,5-dimethylthia~ zol-2-yl )-2,5-diphenyl tetrazolium bromide ) Ee é& i + 5} UN & Boehringer Mannheim 2 Settee 0 HT FRO i F509 AT OAR SRC RPMI 164034 FEE (Gibco ) fi 10 (2 HINA > IX, 10X, 100X, 1000X, 10000X, 100000X x Aff LARS > fA ° 2.94 recombinant human IL-6 — (rIL-6, Boehringer Mannheim Biochemica, Germany) AJ RPMI-1640 $4 #8 #1 10 (9 2 7 $8 FZ FF 1000, 100, 10, 1, 0.1, 0.01, 0.001 U/mL 2 IL-6 #8 8 4 HE > ti LA RPMI-1640 FSO U/mL > HESS AF IL-6 ARM RK File 3. 2h RPMI-1640 3 38 MAAR TTD) cell line F532 FE4 x 104 cell /mL ZIP > fA © 4.4596 FL HE P53 BNA SIRS SE BABU RS MAE Z IL-6 TSO wl > SE — BEE AR NTL © SES SFIS IM A SO RAE IS 4 x 104 cells / mL Z7TDi cell ° 6. 6 FUUFRILE ARE * HRA K © 7, EG HS SFL A 10u1 (Smg / mL) 818 RE ZMTTHRER © 8.45 ME AERA > Diy © 9. & FL IM A 10041 Z SDS (10%, sodium dodecyl sulphate ) + FALAVSAEFL&P 2 fie * FAT ASE PAZ © IOUS EM > OBER RT MAA > BITE BHA 550m © 1, Ay AS AL FR AS IE 7 a PS PAS AT FF ZOD (EP > IBGE HR AE RR (half- maximal growth of cells) IZ OD fii & 4 > RA IL-6 REE RS + ANT RA IL-6 iStE © © Anti-IL-6 $4 #840 5 IL-6 Ft BT RR LAAT SA Mi EMRE IL- 6 > ALLA AB Anti-human IL-6 Ab i 77 04) fF He HARI Et 1. 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