S S V 1/S 1/V

2.5x10-6 0.0000025 24 400000 0.0417

3.3x10-6 0.0000033 30 303030 0.0333
4x10-6 0.000004 34 250000 0.0294 0.045

5x10-6 0.000005 40 200000 0.025 0.04 f(x) = 8.3238468507222

R² = 0.99990573220677
1x10-5 0.00001 60 100000 0.0167
0.035

0.03
2x10-5 0.00002 80 50000 0.0125 0.025
4x10-5 0.00004 96 25000 0.0104 0.02

1x10-4 0.0001 109 10000 0.0092 0.015

2x10-3 0.002 119 500 0.0084

0.01

0.005
1x10-2 0.01 120 100 0.0083 0
0 50000 10000

S S Vo 1/S 1/v
8.33x10-6 0.00000833 13.8 ### ###
1.25x10-5 0.0000125 19 80000 ### 0.08

1.67x10-5 0.0000167 23.6 ### ### 0.07 f(x) = 4.998681476883

R² = 0.9999902633906
2.00x10-5 0.00002 26.7 50000 ### 0.06

0.05
2.50x10-5 0.000025 30.8 40000 ###
0.04
3.33x10-5 0.0000333 36.3 ### ###
0.03
4.00x10-5 0.00004 40 25000 0.025
0.02
5.00x10-5 0.00005 44.4 20000 ###
0.01
6.00x10-5 0.00006 48 ### ###
0
8.00x10-5 0.00008 53.4 12500 ### 0 20000 4000

1.00x x10-4 0.0001 57.1 10000 ###

2.00x x10-4 0.0002 66.7 5000 ###
3.1 x 10-5
2.67x10-5
 y=mx+b

Chart Title
0.045

0.04 f(x) = 8.32384685072223E-08 x + 0.00834805683220909

R² = 0.999905732206773
0.035

0.03

0.025

0.02

0.015

0.01

0.005

0
0 50000 100000 150000 200000 250000 300000 350000 400000 450000

b=1/Vmax m=Km/Vmax
0.0083=1/Vmax 8X10-8=Km/120
Vmax= 120.481928 Km= 0.00001
120 1x10-5

1/v
0.08

0.07 f(x) = 4.99868147688354E-07 x + 0.012502051457195

R² = 0.999990263390638
0.06

0.05

0.04

0.03

0.02

0.01

0
0 20000 40000 60000 80000 100000 120000 140000

y=mx+b b=1/Vmax 0.0125=1/Vmax Vmax =1/0.0125

m=Km/Vmax 5x10-7=Km/80 Km= 0.00004
Vmax= 80
4x10-5