ANALYTICAL PROCEDURES PADDLE TESTERS For fast and easy monitoring of surface and liquid contamination Method uments imporantinchemld exer ood, ‘Sing ih ans santa, hacen mas doe {Tet vurtcesand amnvscos urbe {fate Ths sefeonuinel teow ess ove secretive ous for screening pons. UNIQUE DESIGN ALLOWS ‘SIMULTANEOUS TESTING Inthe same via, wth one convenient incubation. Theda ‘opel clear, akproot container fr incubation. ‘Advantages of Pade Testers 1 Molden grt rcttates ens colony coun 1 Singlet design minnizes contamination and makes 1 Results can be read without opening the i the paddle ensuring color diferentaton and preventing Hach offers thre types of Pade Testers. One side ofthe ‘duatagar pad gies 2 nonselective ttl count of acer The other se fe seletve fora specie gp ot bactei, PADDLE TESTERS FOR TOTAL AEROBIC BACTERIA AND YEAST AND MOLDS ‘The medium on one side contain Tryptone Gicowe Extract Azar with Tepeny! Tetrazohan: Choe TT) Indltor for nonselective growth of total cro foreasy ientieation and enumeration ‘The medium on the othe sie contins Modied Sourid Aga with Rose Benga for the slectve olan of yeas and ms, Rose Bengal stain the yest pink, init fe growing molds rom growing ver Slower growing mols and ius acteal growth lamentous growth indicates mok’s s Incubate the Paddle Teste is an upright poston 2130". Countand record bactral growth on ‘Tiyptone Glucose Extract Age eae 48 nous) ate 48 hours and 120 hours (Seay) of incubation, PADDLE TESTERS FOR TOTAL AEROBIC BACTERIA AND TOTAL COLIFORMS. {The medium tm one side contains Tryptic Soy Agar with {TC naicatr for nonselective growth of wal erode ‘2s Weniicaton and enumeration. s The medium onthe oter side contin Vio Re Tile ‘Aga, amd used fr enumerating ot colons “The presence of total coli in rw fonds er processing nay ince feel contagion ad are Inthe manufacturing proce. Coliforms ferment Ihetone fermenter pace clores ogy clones Mos ram postive onanism are ihe fom growing ‘Incubate the Paddle ester vials in an upg poston at 3540 37°C. Cavan recor bacterial growth on ath Sister 241048 hous of incubation, PADDLE TESTERS FOR TOTAL AEROBIC BACTERIA AND ‘DISINFECTION CONTROL ‘The medium on one side contains Teypic Soy Agi with TTC Indlestor for nonselective growth of toa croc Ince, TF indicator tins bacterial colonies re for ‘sy ientication and enmeration 1 Te medium on the other side contains D/E Newtaizing ‘ar for determining santationefceney and disinfection laf conaisers equipment nd envonental siices et ‘ean, D/E NeualiangAgarneazes sever aseptic nd disinfectant chemicals including quatemey amon omponis, pheno, din, chonne, mere fommldehyde and gutealdehjde. Neuealzaon allows ‘Incubate the Pade Fester vis in an upright positon st 850 47 C. Gout and record bacterial growth on oth Side ater 241048 hous of incubation,PADDLE TESTERS, continued USING PADDLE TESTERS IN LIQUIDS 1. rene 2x Dip he pate paste 4 ncaa te oles USING PADDLE TESTERS ON SOLID AND FLAT SURFACES [Tn a al [= el Ls remem pasite Ze Gentyprewone Be Tne iste inca the 5. coun the clair re a8PADDLE TESTERS, continued INTERPRETING THE LEVEL OF CONTAMINATION ‘The microorganism counts obained with the Pale Testers are semiquantitative. Monitoring with Pade Testers over ern of tne ives good indication of how the microbial loads changing at given ste 100,009 1000109) 00 (109) 00.109 1000 (105 10.000 (10 ca 100.000 00 100.0010 “O Mm 7 i 100 (109 1000 (109 10,0010) qa i i stig Moderne Hes 100,000(10PADDLE TESTERS, continued INTERPRETATION OF RESULTS: ‘Total Aerobic Racteria—tacteria wil gow on the igh ‘ole medi in red colonies, Compare the colony deny ‘wih the bacteria char provided. Each colony represents ‘Total Coliforms —Colfoms will appeas pakish gray Colonies onthe igh ed medium. Canes which appear oles indeate the presence of ponactose fermenting bacteria Most ram posive bacteria ae ibe. 1 Yeast and Mold he pink side wil ndcate the Presence of yeast and nak. Yeasts growin tooth, pin, ‘ound colonies. Molds grow in zz colonies. If n0 {smh appears alter 48 hours, extend incubation for up ta 120 hours 39. ‘= Disinfection ConteolCompare the colony density on ‘ich de ofthe pale withthe acer colony dens et ‘Grow om the Tryp Soy Agar incites that cleaning was rot ffective. Any growth on de D/E Neutra Azar {nicates hat bactera suv cleaning. D/E Newcalzng sell indestesbacteral growth, ‘Lndustial Fuide—Resuks rom scuba analysis of Inds is ea serve arty of sel popes, particule when used 1 monitor contamination of Coola ‘ated tol ie ct dowa on omy proton stops, echice the dspoal cot of usable ids, and optimize the urge of cides. {expensive method Tor monitoring gros changes in water ‘qunataive anass. Pade Testers used ina water can De interpreted dhe same as hose used i indus is 1s Nonviscous Ligulds—When monitoring noviseous Tiquids such as beverages, process Water, sae Water, and ‘ler and coting tower water, Paddle Tester provide ick detemination of bacteria Instead of aping the padi ino the sample, you cin pour Tomlofa wellmixed, died or representative sample of sonviscous ligud elly sto dhe vi Wea using 1a ‘ample, every olny scen repens 50D onanism perm. of Squid tection fate 10,00 every colony represents 500,00 (500 10,08 oganisns pm of hg PRECAUTIONS Proper aseptic techniques must be fllowed when handiag Pale Testers toachieve acurate resis. A snbly contaminated paddle should not be used Range: Paddle Testes measie cob growth i the range of 100 organisms per ml wo 100,000 organisms per ‘Ly comparison 1 colony density char Storage: The Pale Testers shoud be stored at 208°C (6610 46° for optimum shel ie. Freezing andlor ‘esponure to sunlit shoul be avowed. Sock rotation and eatin 6 the expeaton dat are recommended Safety: Never handle media before oe ater use. Used Pade Teer and supplies can be posed of by saad seriiation procedure, Autoclave at 121°C (50°F) at 103 kPa 5 ps) or pour 10 mL of sopeopy alot or concentrated Beach into the ia lore disposal NOW To ORDER Cat. No. Description 216108-10 Total Aerobie Hactsh/Yeast and Mokd Pade Tester, 7p 26109-10 Total Aerobie Bacteria Fotl Colform Pade Testr, 179k 26195-10 Toul Aerobic HactestuDisinfction Cont dle Teter, lvpk 2616400. neubstor Care, 120 Vac 2616402 incubator Culture, 240 Vac 26192-00 incubator, inderounte, 120 Vac 2619202 neubator Undercounter 210 Vac International customers note—Padle Testers haves "emonth sf ld rom die of manure {In the United States, call 800-227-4226 toll-free for current prices or technical assistance. ‘Outside the United States, contact the Hach office or distributor serving you, A