Nome: Annisa Nur Shading cs: [2 Co pue, 2178-2019 DA ’ ‘Class 12 Unit Test - Enzymes Multiple Choice id p \ he Identify the choice that best completes the statement or answers the question - 1. Enzymes are biological catalysts, which speed up reactions. ‘Which statement is NOT correct about mechanism of enzyme activities. @® Enzymes increase the rate of reactions by ¢. Substrates bind to enzymes for reaction increasing the activation energy to happen b. Enzymes speed up the reactions but they d. Some enzymes need cofactors and are not changed by those reactions coenzymes to function properly 2. Inan experiment students prepared four cubes of potato, each of one gram. They boiled two eubes for 10 minutes. They chopped (cut) one of the boiled cubes and one ofthe raw cubes. They labelled the potato Sadens aed each potato sample into one of four est tubes with 1 ral hydrogen peroxide and observed bubble formation, If temperature and pH! were same in all four tes tubes, which could De the correct order of ‘oxygen bubble production from the largest tothe smallest? | a a A=C>B=D @ B=A>C=D B>A>C=D e A=CC>B>A 4. The diagram below shows an enzyme and wo inhibitors of = x | ou b) © - describes the two inhibitors? ee nn %X is a competitive inhibitor and Y is @ a. X and Y are competitive inhibitors non-competitive inhibitor fe. not enough information given the enzyme, X and Y. bX and ¥ are non-competitive inhibitors 2X isanon-competitive inhibitor and ¥ is ‘a competitive inbibitor aaa, Scanned with CamScannerDea ~ Catalase was added to hydrogen peroxide solution. The volume of oxygen produced was ‘mcasured at intervals. The results are shown on the graph ° 10 9 8 ? oxygen 6 produced! § om ‘ 3 1 ce Gm 6 tO ‘ume seconds ‘Whaat was the initial (inthe first 30 seconds) rate of reaction? 2 ad3cm's dd 10.0cm's* © aiosws: : : The enzyme lysezyme secreted from fear glands forms deposits on contact lense. ‘Which ingredient would be effective in a contact lens cleaner for removing these deposits? 2 ethanol pit batters B coenzymes © prowess © cetaceans 4B ‘& Metabolism is defined as all of the chemical reactions taking place in a cell. There are two main types of reactions in cells; anabolic and catabolic. In anabolic reactions __ molecules become _, and these reactions _energy, ‘Which option completes the above sentence correctly? &complen, simpler, need small, larger, release D. complex. simpler, release ©. simple, more complex, release © simple, more complex, need ‘ _—— 2. Which is corregt for enzymes? = They are vitamins and biological, They are no affected by temperature catalyst. changes They arcarboycates and chemical They are specific interaction mesenger ¢. They can be used only once. VY ‘Scanned with CamScanner\ Name: Da Name: —— 8. Diagram shows the as # energy changes during the same reaction with and without cnzymes __ 2 Some a t bh aaa es 4 13. The Hos ‘ b ‘Which labels show the activation energy and the reaction with the enzyme?, je a. Wand 4. UandX __ 14. The b. Wand Y e ZandY © vasy ‘Two samples of a human enzyme were used in an experiment. Before they were used "sample X was heated to 80°C and then cooled to 37°C ~ sample ¥ was cooled to O°C and then heated to 37°C. . ap sues el acttray? E Je X and sample ¥ are no longer. @) Seaside po she ton Semple Xand sempleY willbe equally © eanogh infomation given to make 2 P Conclusion about enzyme activity x active. ‘Sample X will be more active than sample Y. = ‘Scanned with CamScanner1D: A Nam 12, Some bacteria are metabolically active in hot springs (water) because _- a. they are able to maintain a lower internal. their cell wall protects them from high temperature, temperacures b. high temperatures make catalysis, . their enzymes are completely insensitive unnecessary. to temperature. © their enzymes have high optimum temperatures. 13, The temperature of an enzyme-controlled reaction is increased by 10 °C How does this affect the rate of reaction? a, Italways increases the rate. d._Ithas no effect on the rate b. Italways decreases the rate. © may increase or decrease the rate. cc. Ttcause denaturation of the enzyme. —— 14. The graph shows the effect of pH on the rate of reaction of an enzyme. I | | 7 ‘What does the graph show? a. ‘The enzyme is destroyed at pH 9. d. The enzyme works best at pH 6. b. The enzyme is destroyed at pH 2 © The sate of reaction is the same at pH S and pH 8.5. cc. The rate of reaction halves (becomes half) as the pH changes from pH 5 to pH 7. 15, Human amylase enzyme speeds up digestion of starch into maltose. ‘According to the lock and key hypothesis, what is the lock and what is the key for the enzyme amylase? ‘a. The lock- starch, the key-maltose @ The tock- amylase, the key-starch 'b. The lock- maltose the key-amylase The lock- amylase, the key-maltose c. The lock: starch, the key-amylase it changes the shape of the active site, _d. _It increases the kinetic energy of the substrate molecules. bb. Itchanges the shape of the substrate and. Il increases the chances of collisions product molecules. between substrate and product molecules. aes (ie extreme pH (pH far from the optimum) decrease enzyme activity? © _tdecreases the concentration of cofactors. ek ‘Scanned with CamScanneri f Nene WA Naw 17. What isa complex enzyme? a. am enzyme with protein part 4. anenzyme with more than one active site j b. anenzyme with allostericfregulatory site ©. enenzyme with more than one different type of active sites © anenzyine with prosthetic group — is correct for all enzymes? ‘are made inside the cells @ Try break down substrates ‘They are used inside the cells They bind to active sites ‘¢.They change simple substances into more ‘complex ones 19. An enzyme is completely denatured at 50 °C. A fixed concentration of this enzyme is added to a fixed concentration of ts substrate, The time taken for completion of the reaction is measured a diferent Aemperatures. Which grapt shows the results? ‘ 8 Mg : taken time taken ieromeeton for completion of the reaction ofthe reacton 0 40 0 60 10 20 30 40 50 60 5 ° emp tenpeetu °C c D Pe time taken forcomploton {or completion of the reacton ‘of the reaction 10 20 90 40 50 60 1 2 30 40 90 60 ‘temperature °C temperature/*C Scanned with CamScanner1D: A Fails ae an, The effec of substrate concentration onan enzyme-ctalysed reaction was measured in different conditions: ® with no inhibitor * with a competitive inhibitor * with a non-competitive inhibitor: The graph Shows the results ‘enzyme with no inhibitor inhibitor X rate of inhibiter ¥ ‘0 substrate concentration Which statement is correct? Y is a competitive inhibitor which has a ite of the a, Xisacompetitive inhibitor which binds ¢. similar shape to the active toa site other than the active site ofthe enzyme. enzyme. b, Xs anon-competitive inhibitor wt is « non-competitive inhibitor wh finds toa site other than the active hhas a similar shape (0 she-aetTve Site of ar the enzyme, the enzyme. ——~— c. X ise competitive inhibitor which has a similar shape to the normal substrate. 21. A student wrote some notes about enzymes She wrote: Phew ofthe enzyme i... 0 an Fea onthe substrate “Tis area on the substrate can fit ino ito form an... complex” Which words correctly complete gaps? © active site, complemenary, 4 enzyme-substrate setive site, similar, enzyme-substrate ¢, shape similar active site, complementary, cenzyme-product shape , complementary, enzyme-product wzyme-substrate b. 22. Zinc, an essential element for most organisms, is present in the active ste ofthe enzyme carboxypeptidase. Fee roogt ely factions a ao) — @ cofector secessiy for enzyme activity ‘competitive inhibitor of the enzyme. ‘coenzyme necessary for enzyme act a . noncompetitive inhibitor of the enzyme. activator of the enzyme. a \ 6 Scanned with CamScannerE -: PPM Nur Shadrina (362) val-0 | SPI SM AN BANUA KALSEL BBS EN LALAN TSNGAH SE ER @) INGAH SEMESTER GANJIL ; i AHUY AJARAN 2019 - 2020 BIOLOGIKELAS 12! ae: 5 DURASI 20 Menit Multiple Ch Identify the choice that best completes the statement or answers the question 1. Which enzyme is responsible for carbon fixation in mesophyll cells of C4 plants? a. Rubisco 4. ATP synthase © PEP carboxylase ©. PEP ©. RuBP 2. Diagram shows an organelle, In which labelled part does the Calvin cycle reactions take place? a 1 ‘stroma a. 4 ®2 G3 fe 3) 4, Which ofthe following are products ofthe light stage of photosynthesis that are ‘used in the Calvin cyele? a. CO,and ATP d. electrons and O; ATP b. HO and O> e. NADPand ATP NADPH © ATPand NADPH 002 1 Scanned with CamScannerName PAnoicaiur sh ado (a wut A cs a Weal 4. Following ore staresments abput potesprithe us ' J Yfhapptas in sug | ' | ’ . produce €O2 2. produces oxygen ' Y4-inclucies Ga'bon fixation ' 1 ' YS. prodiices sugar A Which statements are correct for the dark reactions of pli hotosynthesis? a. LandZ 4. 1,Zand 4 b. Land 4 © 1,4 and 5 c. J,4and5 © Patrick set up the following experiment ina dark room. + collected bubbles — = buh ; fet ladaal = Za cee feseal oie thet We During the experiment, he changed the distance between glass shield and the bulb and observed the ‘number of bubbles produced per minute for each distance. Which statement about the above experiment is correct? a. temperature is the independent variable d. _as the distance increases number of in this experiment X bubbles produced by Elodea increases b. light intensity is the dependent variable _¢. glass heat shield is used to change the in this experiment wavelength of light for Elodea © asthe distance increases, light intensity for Elodea decreases Scanned with CamScannerA ome: Aonisa ur Sddrina (362) xil-e | t a | 1 | are ' 1s a Dey 7 6, Pisran hows a sage of phot aA rape it | ea ' & ' meet prop (yi yh \ Pe? ok ’ ' ’ Uk Ts Y Men 4 ’ ' \ ‘ [ > Based on the diagram, what are X, Y and Z? a. RuBp, Rubisco, PGAL, @ RuBP, PGA, PGAL b. Oxaloagetate, Citric acid, Pyruvate ¢._ PP, PEP carboxylase, PGAL- c. Oxaloagetate, Acetyl CoA, Citric acid, Pyruvate 7. Metabolism is defined as all of the chemical reactions taking place in a cell. There are two main. types of reactions in cells; anabolic and catabolic. @ amino acids —> protein i. Glucose — lactic acid ji, 2H,0 > 02+4H" (ot jv. X > Y+Energy aot gv Which of the above reaction(s) is(are) anabolic? @ only 4. ii,iii andiv b. ii only ©. iandiv c. ijiiandiv 8. Salivary amylase enzyme works well in the mouth where pH is neutral, but in the stomach with acidic pH values its activity stops. Why does amylase enzyme activity decrease at very low pH environments? @® Shape of the active site changes. 4. Kinetic energies of the substrate and enzyme molecules decrease. b. Concentration of the substrate decreases ©, Chances of collisions between substrate and product increase. c. Concentration of cofactors decreases. Scanned with CamScannerin 12. isa Nur Sharing fabs) j 2 | ( \" edited a, | ih | mg Senittaiescoget seeker | investigation was Substrate solutions cud Out on the effect of pH on an enzyme-catalysed reaction, table ns were placed into five separate test-tubes and pH of each test tube is arranged as in the peas at Tare fad volume of enzyme was added to each test tube The meee ane Ket forthe tine tated Product formed was then measured 6, b Scesrieve 859 li esperievantpal -] toss of pode ad ge 30 5 = 0c “8 BS 4 10 ao) 6 48 os a 600 = = : a . oy a 10 3a 4 At which pH was the rate of reaction highest? ant ® 2 8 b 4 © 10 «8 Diagram shows mechanism of lactase enzyme activity, Ax Which statement is NOT correct about the diagram? os a, T shows the products glucose and 4. Shape of X is complementary to shape of galactose lactose ¥ b. Wis lactose-lactase complex ¥ e. Zan be used again and again © Yislactase Which molecule is the final electron acceptor in lactic acid fermentation? a. glucose lactic acid © pyruvic acid e. ethanol ©. acetaldehyde 4 Scanned with CamScannerAndisa NuéShadrina (362) ' uy ' \: XIE - { { r | ree (t ata Following gap shows these ofan ymca roll ‘an enzyme-catalyzed reaction as reactant (substrate) concer cha: ges, with the concvnratiog of endmeconstitia fo Ply fn s1004 paleey 1 yaa ‘| 490 epee pS ae | * +80 ’ ' 470. 3 a 420 440 4 460 480 +100 «+1200 Concentration of reactants, micromolar In the figure, why does the reaction rate become almost constant at higher reactant concentrations? a, Kinetic energy ofenzyme decreases d._enzyme gets denatured © Most enzyme molecules are occupied by ¢. enzyme gets inactivated substrate cc. The activation energy for the reaction jnereases with reactant concentration. 14, The diagram summarises how glucose can be used to produce ATP, without the use of oxygen. glucose XY in mammals (lactate) | anoerobic Zin yeast (ethare) Which compounds are represented by the letters X, Y and Z? a. ethahol, pyruvate and lactate d. pyruvate, ethanol and lactate b. pyruvate, C2 and alcohol © pyruvate, lactate and ethanol cc. lacyhte, ethanol and CO2 During aerobic respiration in eukaryotic cells, a high proton (H+) concentration accumulates in a, stroma (Phd) matrix (aertae) b. thylakoid space (pho) © intermembrane space ¢. cytoplasm Even though plants do photosynthesis, plant cell till use their mitochondria for aerabic respiration, When ‘and where will this occur? a inphotosynthetic cells inthe light, while @ in all cetls all the time photosynthesis occurs . in nonphotosynthesizing cells only e, _inphotosynthesizing cells in the light and in other tissues in the dark c. _ incells that are storing glucose only Scanned with CamScannerCo Hinde + 60, —6C02 +, BH20 ’ Y Mic { { Dy ad | Ss] ——, 17. How mar { ih } iit } Lan t PS Pe oe) ee J time a molecule of glucose (COH1206) is i ae broken down to CO? bnd 120 via aerobjé x Spiratioh? | ook sob ie » fa. i2 ee rie yo es Me During aerobi P ® dws ing aerobic respiration, electrons travel downhill in which sequence? oe aor citric acid cycle» ATP» @)_glucose-»ATP—> electron transport 5 chain» NADH slucose-> NADH electron transporte. glucose» glycolysis citric acid chain— oxygen cycle» NADH-> ATP ©. glucose» pyruvate-> ATP-> oxygen 19. A gardener is concerned that his greenhouse is getting too hot from too much fight, and wants to shade his plants with colored translucent plastic sheets. What color should he use to reduce overall light energy, but still maximize plant growth? a. green d. orange b. yellow e. any color will work equally well © red 20. Which statement is correct? a. C4 plants have stomata which are open at d. C3 plants are best adapted to hot and dry night. conditions. * b. CAM plants have Calvin cycle occurring @ Only C3 plants have rubisco enzyme for in bundle-sheath cells. carbon fixation ¢. Com and sugar cane are examples of C4 plants. ____ 21. Which is a simple, permanent tissue? a. xyle (ompied) aeeaite 4. apical merisfem b. phloem \“ ce. vascular cambium (masuk ke lateral meristem) © epidermis 22, Which statement is NOT correct about the cells found in vascular bundles of dicot plants? ‘xylem vessel elements transport water d._ phloem sieve tubes are living cells V ‘and mineral salts ¥ © _tracheids transport products of e. companion cells can’t do transportation thesis of any material cc. cambium cells are responsible for secondary growth V Scanned with CamScanner24, ‘a \ h labels point to the zone of cell differentiation and the root cap? a. ZandM a XandM b. YandM © XandN ©. YandN Plant hormone __ causes seed dormancy, whereas another plant hormone _ @® abscisic acid, gibberellin ABA d. cytokinin, ethylene b. gibberellin, auxin ethylene, abscisic acid c. auxin, cytokinin Following a some curate of pl snes pac cells have uneven cell walls, thick at comers (€) A made of living cells (pC) cv IHl- cell wall contains cellulose and lignin & s TV- made of actively dividing cells ue (p) V- can function as a storage Which of the above characteristics are true for the pee tissue shown below? i ] i a. TandIl d. Wand b, Mand Ill e. I IVandy © 1, Mand iil I 1 Which ofthe following describes the to sand ¥ are competitive inhibiors. (4) Xia. competitive inhibitor and Y is ‘non-competitive inhibitor fe Xisanomcompettive inhibitor and bX and ¥ ae non-competitive Into Visa compentive inhibitor Xisarea inhibitor and Y isa preudo ‘ahibtoe 6, Carbon dioxide released during which of te following stages ofseobic respiration! 2 syeqfhic andthe oxidation of | &_sheaffsisand Krebs cle Prodiioscay Con © aeternaetcl Cah «extn py acy CoA sinetectioae een en sro e. Easvojde el deren tort = Scanned with CamScannerName: 7+ Diagram shows the Calvi eee ene Bo, x 1 Galvin Cycle ee ua ~ Y CROCCO Bae ont Saran, ht we XY? up nd PGA, 4 9h anche fund PGA f Mtmicinoe ‘© RuBp and Ghose "GS ontns:chomins conse nd" "elope Cromoses urge YC Became chrmesomes thems athe equator of el 1 mtupse ncesrmembane ——&_pophse-cesing ver kes lice YX rpg «Sep ___ 18. In which stage of call division Wbmologous chromosomes exchange material to produce recombinant chromosomes? 4. prophase I of meiosis prophase of mitosis of DNA 20% ofthe bases ae adenine. fsine bases in the same piece of DNA? percentage of ex ome 15. Which is correct for the nitrogenous base weil? ublering structure @. itis pyrimidine ‘hymine itis found only in € itjoins its complementary base by ‘thre hydrogen bonds 16 Heticase enzymes synthesize DNA © sept te 0 DNA sands © beak and jin DNA Scanned with CamScannerNome wma ‘Ween ro engaged es wre ma he ftring inched 7 wih ag wings md 24 wi even SSR eegpe cept Tcecenegp omnes ¢ Ontheteg—se Gata se = wee + Boo honor meat eee : 1. 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