Genetic Morbus Bechterew Risk

Analysis of the HLA B27 alleles

Major Histocompatibility Complexes (MHC) code for a multitude of histocompatibility

antigens (HLA). These antigens determine the immunological individuality of each human.
It is well known that certain diseases are strongly asscociated with specific HLA profiles - so
it is also in the case of Morbus Bechterew (ankylosing spondylitis) and HLA B27 alleles. In
Europe, about 10 – 15 % of all persons are HLA B*27 – positive.
Therefore, the detection of HLA B*27 alleles is performed as pre-screening for potential
M. Bechterew patients and 95 % of all patients are positive. Subsequent subtyping of positive
samples in this pre-screening allows a further differential diagnostic.
Especially individuals with unclear symptoms for the disease benefit
MutaGEL® HLA B27
(KT0003) (for research use only) from this procedure. About 2-5% of all HLA B2*27-positive persons
develop M. Bechterew and only 5% of all patients remain B*27-
 Allows the the detection of HLA B*27 negative. By detection of HLA B*27 alleles the individual risk for this
allels in an easy and effective way (ready chronic inflammatory disease can be estimated: about 90 - 95% of all
to use PCR tubes) M. Bechterew patients carry the subtype *2705 – the other ones are
*2702- or *2704-positive.
 Allel-specific PCR test
Subtyping as follow up in case of positive HLA B*27 status is done
 Sample material: isolated DNA (e.g. over specialized laboratories, f. e. by sequencing methods. Indivi-
extracted from whole blood, Sputum) duals with lower risk (or with mild and retarded courses of the
disease) will be defined from individuals with the high disease
association mentioned above. Please contact Immundiagnostik for
further information.
The MutaGEL® HLA B27 test (KT0003) from Immundiagnostik
allows the detection of HLA B*27 allels in an easy and effective way
(ready to use PCR tubes). The pre-screening test-kit is sufficient for
24 determinations. A small sample of EDTA-blood is necessary for
a preliminary extraction of DNA (isolation reagents are not inclu-
ded) which is used as matrix in the kit. The subsequent processing
with molecular biological methods leads to specific amplification
products detectable by gel electrophoresis.

US: all products: Research Use Only. Not for use in diagnostic procedures.
FLMB13 November 2015

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