3.5.05 before end point is reached.) Stdze KMnO4 as in AppC.

i
AOAC Official Surplus Method 921.03i 955.57. Calc. wt sugar from table, 11th ed.
Sugars (Reducing) in Plants
References: J. Am. Chem. Soc. 43, 1503(1921);
Quisumbing-Thomas Method JAOAC 15, 71(1932).

Final Action © AOAC INTERNATIONAL

Surplus 1970

31.048-31.049, 11th ed.

31.048 Reagents

(a) Copper sulfate soln.—Dissolve CuSO4C5H2O in

hot H2O to make satd soln, and filter. Det. Cu
electrolytically and dil. soln so that 25 mL contains 525
mg Cu (41.2 g CuSO4C5H2O/500 mL soln).
(b) Alkaline tartrate soln.—Dissolve NaOH in equal
wt H2O and let stand several days until insol.
carbonates and other impurities settle out. Siphon off
clear soln and det. its alky by titrn with std acid.
Dissolve 173 g highest purity KNa tartrateC4H2O
(Rochelle salt) in H2O in 500 mL vol. flask and add
calcd quantity NaOH soln so that 500 mL of this alk.
tartrate soln contains exactly 65 g NaOH. Dil. to vol.
with H2O.
(c) Ferric ammonium sulfate soln.—Add 200 mL
H2SO4 to ca 500 mL H2 O and cool. Dissolve 240.9 g
FeNH4(SO4)2C12H2O in the dil. H2SO4 and dil. to 1 L.
(d) Indicator soln.—Dissolve 0.15 g o-
phenanthrolineCH2O and 0.07 g FeSO4 in 10 mL H2O.

31.049 Determination

Measure exactly 25 mL each of CuSO4 and alk.

tartrate solns into 400 mL Pyrex beaker, diam. ca 9 cm.
Add 50 mL sugar soln contg preferably 50–150 mg
sugar. Cover beaker with watch glass and place in H2O
bath held at 80E.
After digesting exactly 30 min, filter Cu2O by
suction thru mat of asbestos in gooch. Wash ppt with
H2O; then with aid of stirring rod transfer asbestos mat
and Cu2O back into beaker in which reduction took
place. Rinse inside of crucible and lip of beaker with 10
mL FeNH4(SO4)2 soln from pipet. Receive rinsings in
beaker contg the Cu2O. Holding crucible over beaker,
stir contents of beaker thoroly with stirring rod until
Cu2O dissolves. Wash crucible with ca 25 mL hot H2O
(80E), receiving washings in beaker. Stir contents of
beaker and then raise beaker to see if any undissolved
particles of Cu2O are resting on bottom. If so, press
each one with point of stirring rod until all dissolve.
Add ca 125 mL addnl hot H2O and 1 drop indicator
soln. Titr. at once, with continual stirring, with 0.05N
KMnO4. (In long titrn, preferably add indicator just