DXH 500 Series FSM - B44425ac

Service Manual
DxH 500 Series
B44425AC
March 2018
Manufacturer
Beckman Coulter Ireland Inc.
Lismeehan
O’Callaghan’s Mills
Co. Clare, Ireland
This Service Manual contains confidential information of
Beckman Coulter, Inc. and its receipt or possession does
not convey any right to reproduce, disclose its contents, or
to manufacture, use, or sell anything it may describe.
Reproduction, disclosure, or use without specific written
authorization of Beckman Coulter, Inc. is strictly forbidden.
Beckman Coulter, Inc. makes no representation that, upon

furnishing this service manual, the holder of the manual
will have the necessary technical capabilities and know-
how to properly troubleshoot and repair any of the
equipment specified in the manual. Beckman Coulter, Inc.
assumes no liability whatsoever, including consequential
and incidental damages, resulting from improper
operation of Beckman Coulter instruments after
maintenance of Beckman Coulter instruments has been
performed by persons not employed by Beckman Coulter,
Inc. Furthermore, Beckman Coulter, Inc. assumes no
liability whatsoever for any personal injury or property
damage resulting from maintenance and/or repair of
Beckman Coulter instruments performed by persons not
employed by Beckman Coulter, Inc.
Trademarks
©2017 Beckman Coulter. All rights reserved.
Beckman Coulter, the stylized logo and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services

are trademarks or registered trademarks of their
respective holders.
Revision History
Initial Issue, PN B44425AA, 10/2015
Issue AB, /2016

Chapter 8 - Parts Lists were removed and incorporated into a separate manual: DxH 500, Parts Lists, PN B93296.
Issue AC, 03/2018

Added the DxH 520 instrument
Updated:
Safety Notice
1.2, Safety Precautions (added California Proposition 65)
2.1, Introduction to the DxH 500 and DxH 520 Analyzers
2.2, Principles of Operation
2.3, Diluter System
3.1, Pre-Installation Checks
3.2, Unpack and Inspect the Instrument
3.3, Connecting Waste and Reagents
3.4, Connecting the Reagents
3.5, Connecting the Instrument's Electronic Components
3.7, Waste Setup
3.8, Reagent Setup
3.13, Daily Checks
3.14, Check Instrument Settings and Adjustments
Power Up/Power Down (4.1, Guidelines for Servicing Instrument)

Backup or Restore [Software v2.0 and future releases] (4.1, Guidelines for Servicing Instrument)
4.2, Doors and Covers Removal
4.3, Front Cover Assembly and Components Removal
Removal of Front USB Board (4.3, Front Cover Assembly and Components Removal)
4.4, Power Supply Replacement
Installation of Mainboard (4.5, Mainboard Replacement)
4.6, Display Module Assembly Replacement
4.7, Aperture Gain Checks and Adjustments, “RBC Gain Adjustment”
4.8, Heater Assembly Removal
4.9, Sampling Probe/Rinsing Head Replacement
4.10, Sampling/Rocker Assembly Removal (DxH 500)
4.11, Sampling/Rocker Assembly Removal (DxH 520)
4.12, Rocker Motor Replacement
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.
B44425AC iii
Revision History
Updated:
4.13, Rocker Position Sensor Replacement
4.14, Probe Motor Replacement
4.15, Probe Position Sensor Replacement (DxH 500)
4.16, Probe Position Sensor Board Replacement (DxH 520)
4.17, Probe Belt and Probe Carriage Replacement (DxH 500)
4.18, Probe Belt and Probe Carriage Replacement (DxH 520)
4.19, RBC Counting Chamber Replacement
4.20, WBC Chamber /Optics Bench Replacement
4.21, HGB LED Replacement
4.22, Counting Manifold Assembly Replacement
4.23, Solenoid Valve Replacement
4.24, Drain/Mixing Valve Manifold Replacement
4.25, Syringe Body/Pistons Replacement
4.26, Syringe Assembly Replacement

4.27, Syringe Optical Sensor Replacement
4.28, Syringe Valves Manifold Assembly Replacement
4.29, Tubing Replacement
4.30, Tube Holder Module Replacement (DxH 520)
4.31, Shear Valve Module Replacement (DxH 520)
4.32, Shear Valve Disassembly/Reassembly (DxH 520)
5.1, Maintenance Requirements

5.2, Preventive Maintenance Inspection
Table 5.2-1, Preventive Maintenance Inspection (PMI), “Cleaning the Baths”
7.1, Diagnostics
7.2, General Troubleshooting
7.3, Error Message List
A.1, Tolerances and Limits

B.1, Torque Specifications
C.2, Analysis Cycles - Sequence Description
Added:
CAUTION under section 3.5, “Connecting the External Power Supply”
IMPORTANT under section 4.7, “Optic LED Adjustment and WBC/ALL Check”
Check the WBC Bath Filter (DxH 520 Only) (3.14, Check Instrument Settings and Adjustments)
Touchscreen Calibration (4.6, Display Module Assembly Replacement)
4.35, DI Water System Flush
DxH 520 Timing Chart (C.2, Analysis Cycles - Sequence Description)
D.1, DxH 500 Series Post-Procedure - Verification Matrix (APPENDIX D)
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.
iv B44425AC
Safety Notice
Alerts for Danger and Caution
Throughout this manual, you will see the appearance of these alerts for Warning and Caution
conditions:
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis.
Safety Precautions
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument.
Beckman Coulter, Inc. urges its customers and employees to comply with all national health and
safety standards such as the use of barrier protection. This may include, but it is not limited to,
protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or
any other automated laboratory instrument.
B44425AC v
Safety Notice
Safety Precautions
WARNING
Risk of injury and/or biohazardous contamination. The aspiration probe is sharp.
To avoid being pierced by the aspiration probe, use caution when performing
procedures involving the probe.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the product manuals.
• You introduce software that is not authorized by Beckman Coulter into your
computer. Only operate your system's computer with software authorized by
Beckman Coulter.
• You install software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.
• You do not scan removable media (USB flash drive) before connecting it to a
computer. Always scan removable media.
vi B44425AC
Contents
Revision History, iii

Safety Notice, v
CHAPTER 1: Introduction, 1-1
Scope, 1-1
Customer Documents, 1-1
Service Manual Updates, 1-1
Intended Audience, 1-1
Organization, 1-2
Numbering Format, 1-3
Signal Words and Special Headings, 1-3
WARNING, 1-3
CAUTION, 1-4
IMPORTANT, 1-4
NOTE, 1-4
Conventions, 1-5
Special, 1-7
Electronic, 1-8
Biological, 1-8
Troubleshooting, 1-9
Operational Hazards, 1-9
Hazard Labels, 1-10
Disposal of Electrical Instrumentation, 1-10
Waste Disposal Warning, 1-11
CE Mark, 1-11
RoHS Notice, 1-11
China RoHS Caution Label, 1-12
China RoHS Environmental Label, 1-12
California Proposition 65, 1-12
CHAPTER 2: Instrument Description, 2-1

Summary, 2-1
The DxH 500 Series Systems, 2-1
Components (DxH 500), 2-2
Components (DxH 520), 2-2
Accessories, 2-3
Software, 2-3
B44425AB vii
Contents
Analytical Reagents, 2-4

Functional Description, 2-4
Specimen Preparation (DxH 500), 2-4
Specimen Preparation (DxH 520), 2-5
Counting/Sizing/Optical Measurements, 2-6
Spectrophotometry Measurements, 2-6
User Interface and Device Interaction, 2-6
Summary, 2-9
Spectrophotometry, 2-9
Impedance Measurements, 2-9
Coincidence Corrections, 2-10
Voting and Averaging, 2-11
RBC and PLT Histograms, 2-11
WBC Optical and Impedance Measurements, 2-11
Differential Measurement, 2-14
Data Acquisition, 2-15
Measurands and their Derivation, 2-16
Overview, 2-19
Main Components, 2-19
Probe Movements, 2-20
Horizontal Probe Movement, 2-20
Vertical Probe Movement, 2-21
Sample Probe, 2-21
Rinsing Head, 2-21
DxH 500, 2-21
DxH 520, 2-22
Hydraulic / Pneumatic System, 2-22
Main Modules, 2-22
Rocker Module, 2-23
Counting Module, 2-23
Drain Valve Module, 2-23
Electro Solenoid Valve Module, 2-23
Syringe Assembly (Single Block Assembly), 2-23
Tube Holder Module, 2-24
Shear Valve Module, 2-24
Reagent Transfer Cycles, 2-25
Diluent, 2-25
Lyse, 2-25
Cleaner, 2-25
Waste, 2-25
Pneumatics System, 2-26
Vacuum (Negative Pressure from Pressure/Vacuum
Syringes), 2-26
Pressure, 2-26
Count, 2-27
Aperture Sensing System, 2-27
HGB Parameter, 2-28
Power Supply, 2-29
Mainboard, 2-29
viii B44425AB
Contents
Connectors and Cables, 2-30

Battery, 2-30
Interface Board, 2-31
CHAPTER 3: Installation Procedures, 3-1

Electrical Requirements, 3-1
Power Consumption, 3-2
Carton Arrival and Condition, 3-2
Operational Ambient Temperature, Humidity and Altitude, 3-2
Space and Accessibility Requirements, 3-3
Drainage, 3-3
Installation Supplies, 3-3
Connecting Waste Line to Waste Container, 3-7
Connecting Waste Line to Sink, 3-8
Connecting Diluent, 3-11
Connecting Lyse Reagent, 3-12
Connecting Cleaner Reagent, 3-13
Connecting the Bar-Code Scanner, 3-15
Connecting the Printer, 3-15
Connecting the External Power Supply, 3-16
System Configuration, 3-37
Check the WBC Bath Filter (DxH 520 Only), 3-37
HGB Adjustment, 3-41
Optic LED Adjustment and WBC/ALL Adjustment, 3-41
RBC Gain Check, 3-41
Check Tube Pierce Position (DxH 520 Only), 3-41
Repeatability, 3-42
Calibration, 3-42
Controls, 3-42
CHAPTER 4: Service and Repair Procedures, 4-1

General, 4-1
Guidelines, 4-1
Diagnostics, 4-2
Door Interlock, 4-2
Power Up/Power Down, 4-2
Backup or Restore [Software v2.0 and future releases], 4-3
How to Open and Remove the Left-Side Door [Reagent
Compartment Door], 4-5
How to Open and Remove the Right-Side Door [Fluidic Door], 4-6
How to Remove the Top Cover, 4-7
How to Remove the Reagent Plate, 4-8
How to Remove the Secondary Reagent Plate, 4-9
Removal of Front Cover, 4-11
Removal of LCD, 4-14
Removal of Front USB Board, 4-15
Removal of ON/OFF Board, 4-16
Removal of Start Analysis Plate/ Switch (DxH 500 Only), 4-17
Removal of Mainboard, 4-24
B44425AB ix
Contents
Installation of Mainboard, 4-26

Replacement of Interface Board, 4-27
Touchscreen Calibration, 4-29
Optical LED Adjustment, 4-31
WBC/ALL Check, 4-31
WBC/ALL Gain Adjustment, 4-33
RBC Check, 4-34
RBC Gain Adjustment, 4-36
Rinsing Head O-Ring Replacement, 4-43
Removal, 4-55
Installation, 4-56
Prerequisites, 4-81
Tools/Supplies Needed, 4-81
Removal, 4-82
Installation, 4-82
Disassembly, 4-105
Reassembly, 4-106
Tube Holder Adjustment, 4-113
Piercing Position Adjustment, 4-114
Probe Bath Position Adjustment, 4-115
Tools/Supplies Needed, 4-119
Procedure, 4-119
CHAPTER 5: Maintenance Procedures, 5-1

Sample Probe and Probe Rinse Head, 5-1
Syringe Motor Lead Screw Lubrication, 5-1
Bleaching, 5-1
Vacuum Tube Inspection/Replacement, 5-1
CHAPTER 6: Schematics, 6-1

(TP1) +24VDC External Power Supply Input, 6-33
(TP2) +24VDC Output of Protection IC, 6-33
(TP3) +24VDC Output of Main Power Switch, 6-34
(TP4) +12VDC Output DC/DC Converter for Backlight Power
Supply, 6-34
(TP5) +3.3VDC Output of DC/DC Converter Power Supply, 6-35
(TP6) +5VDC Output of DC/DC Converter Power Supply, 6-35
(TP7) +3VDC Lithium Cell, 6-36
CHAPTER 7: Troubleshooting, 7-1

DxH 500 Valves, 7-2
DxH 520 Valves, 7-3
Service, 7-3
CBC Thresholds Flags, 7-4
Diff Thresholds, 7-5
Diff Flags, 7-6
Adjust WBC, 7-7
Adjust Others, 7-8
Internal Settings, 7-9
x B44425AB
Contents
Burn In, 7-9

CP Adjust (DxH 520), 7-9
System Config, 7-10
CHAPTER 8: Parts Lists, 8-1

APPENDIX A: Quick Reference Information, A-1
APPENDIX B: Specifications, B-1
APPENDIX C: Fluidic Parameters, Analysis Cycles, Tubing List, C-1
DxH 500 Timing Chart, C-3
DxH 520 Timing Chart, C-4
APPENDIX D: Verification Matrix, D-1

Glossary
B44425AB xi
Contents
xii B44425AB
CHAPTER 1
Introduction
1.1 Manual Description
Scope
This manual provides the reference information and procedures needed for repairing and
maintaining the DxH 500 and DxH 520 analyzers. It is available in two electronic versions:
• HTML - recommended for viewing because it is quicker to open and easier to navigate.
• PDF - recommended for printing because it has a normal page layout and numbering.
Beckman Coulter and authorized non Beckman Coulter users can access and download this manual
from the BCI Airwatch site. Non Beckman Coulter users must contact their local Beckman Coulter
Service Manager for authorization to access the Beckman Coulter Airwatch site.
Customer Documents
This manual is to be used in conjunction with any customer documents for the instrument and does
not contain information and procedures already covered in them. The table below lists the
customer documents that are available now or will be available.
Language IFU Host Transmission RUO

English (EN) B23922 (DxH 500) B38989 NA
English (EN) B85528 (DxH 520) B38989 B38966
Service Manual Updates

Any Service Modification that affects the information in this manual will include a Notice of
Information Update form for this manual. A Notice of Information Update form will summarize the
changes and will list the specific headings, figures, and tables affected.
Intended Audience
To use this manual effectively, you need the following:
• An operator’s knowledge of the DxH 500 and DxH 520 analyzers.
B44425AC 1-1
Introduction
• Beckman Coulter authorized service training on the DxH 500 and DxH 520 analyzers.
• A thorough understanding of —
— Basic electronic and pneumatic principles and devices
— Hematology terms and concepts
— The Coulter Principle
— VCS technology
— COULTER Histogram Differential and Interpretive Reporting
— Cytometry terms and concepts
— Reagent systems
— Quality control
— Troubleshooting concepts and techniques.
• The ability to —
— Read pneumatic/hydraulic schematics and understand related terminology
— Read electronic schematics and understand related terminology
— Use a digital voltmeter (DVM) and an oscilloscope
— Use basic mechanical tools and understand related terminology.
Organization
The material in this manual is organized into eight chapters, an appendix, a list of abbreviations,
and a glossary.
To make it easier to access the information:

• In the HTML version, each screen has -
— A Contents button linked to a master table of contents.
— An Illustration button linked to a list of illustrations.
— A Tables button linked to a list of tables.
— An Index button linked to an alphabetic index.
• In the PDF version -
— Bookmarks linked to the main headings are listed on the left side of the screen.
— A master table of contents (including master lists of the illustrations and the tables) is
included at the beginning of the manual and an alphabetic index at the end.
CHAPTER 1, Introduction, includes a brief description of this manual, a list of supporting

documents, and essential safety information.
CHAPTER 2, Instrument Description, includes an introduction to the instrument with the function
and location of each major component, an explanation of the instrument’s operation by system, and
available options.
CHAPTER 3, Installation Procedures, contains the procedures used to install the DxH 500 and DxH
520 Analyzers.
1-2 B44425AC
Introduction
1.1 Manual Description 1
CHAPTER 4, Service and Repair Procedures, contains the procedures used to service and repair the
instrument. Each procedure includes a purpose, a list of needed tools and supplies, instructions, and
a verification procedure.
CHAPTER 5, Maintenance Procedures, contains the procedures used to maintain the DxH 500 and
DxH 520 analyzers and to verify the system is operating correctly.
CHAPTER 6, Schematics, provides schematics, diagrams, and layouts needed for servicing the DxH
500 and DxH 520 analyzers.
CHAPTER 7, Troubleshooting, contains problem descriptions, including a complete list of error

messages, with troubleshooting actions and tips. Use the information in this chapter in conjunction
with the procedures in Chapter 4 to locate and correct instrument problems.
The Parts Lists originally contained in Chapter 8 were updated and published as supplemental
information in the following publication: DxH 500/DxH 520, Parts Lists, PN B93296.
Quick Reference Information, For Background Limits, Carryover and Repeatability, refer to the DxH
500 and DxH 520 IFU manuals.
Specifications, contains torque specifications.
Fluidic Parameters, Analysis Cycles, Tubing List, contains a typical analysis cycle and tubing list.
Glossary contains a collection of specialized terms, with their meanings, either used in this manual
or related to the information in this manual.
Numbering Format
Each chapter of this manual is further divided into topics that are numbered sequentially,
beginning at one. The numbering format for the topic heading, which is called the primary heading,
is chapter number, decimal point, topic number. For example, the primary heading number for the
fifth topic covered in Chapter 2 is 2.5.
The figure and table numbers are tied directly to the primary heading number. For example, the
first figure under Heading 2.5 is Figure 2.5-1 and the first table under Heading 2.5 is Table 2.5-1.
NOTE In the PDF version, primary headings always begin on the top of a right-hand page.
Signal Words and Special Headings

Throughout this manual WARNING, CAUTION, IMPORTANT and NOTE headings may be used to
indicate potentially hazardous situations and important or helpful information.
WARNING
The signal word WARNING is displayed in an orange signal panel and the associated text (in this
example, the definition of WARNING) is in bold-face.
B44425AC 1-3
Introduction
WARNING
WARNING indicates a potentially hazardous situation, which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis (does not apply to all
products).
In this document the signal word WARNING is only used to indicate the possibility of personal
injury. It is not used to indicate the possibility of erroneous data.
CAUTION
The signal word CAUTION is displayed in a yellow signal panel and the associated text (in this
example, the definition of CAUTION) is in bold-faced type.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis (does not apply to all products).
In this document the signal word CAUTION is used to indicate the possibility of damage to the
instrument or of erroneous data.
IMPORTANT
The signal word IMPORTANT is in bold-face and the associated text (in this example, the definition
of IMPORTANT) is indented if it wraps.
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
The signal word IMPORTANT replaces the word ATTENTION used previously in service manuals to
draw attention to information that is critical for the successful completion of a procedure and/or
operation of the instrument.
NOTE
The signal word NOTE is in bold-face and the associated text (in this example, the definition of
NOTE) is indented if it wraps.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
1-4 B44425AC
Introduction
1.1 Manual Description 1
Conventions
This manual uses the following conventions to make the material clearer and more concise.
• Messages that appear on the System Manager screen are in italics.

• In the parts descriptions in Chapter 8, any alternative names, resource IDs, and/or reference
designators are in italics and enclosed in parentheses
• Selections that appear on a screen are in bold face.
• Information that is to be typed (entered) is in bold face and italics font.
• Keys on the System Manager or on a laptop computer are boxed, like this: .
• Keys that are to be pressed simultaneously are printed with a plus sign between the keys, like
this: + + .
• The software path to a specific function or screen appears with the greater than ( > ) symbol
between succeeding screen options, like this: Menu > SMS > Diagnostic Procedures tab.
• When the word "select" is used in conjunction with a screen and the associated buttons on the
screen, you are to press the buttons on the touch screen or click on the buttons with the mouse,
as necessary, to display the desired screen or to initiate the desired function.
NOTE The verb “press” is reserved for mechanical switches such as keys on a keyboard and buttons on
a circuit board.
• Links that display a “flyover” on the screen in the HTML version of the document are green and
are underlined.
• Sections that contain entirely new content are flagged with a New Section icon at the
end of the section title.
• Links to information in another part of the document for additional information are in blue and
are underlined. To access the linked information, select the blue, underlined text.
• For quicker access, the material is divided into many small sections (electronic files).
— Every primary heading is a separate file and whenever possible the amount of material
contained within one primary heading is limited to four to ten pages.
— If a primary heading must be large, invisible breaks are added to the HTML version to
further divide it.
NOTE These breaks are only apparent if you are scrolling through a section. Use the right and left
arrows on the navigation bar to move from one section (electronic file) to the next in the HTML
version of the manual.
• Unless otherwise noted, left/right orientation to components is presented as if you are looking
at the front of the instrument.
B44425AC 1-5
Introduction
1-6 B44425AC
Introduction
1.2 Safety Precautions 1
1.2 Safety Precautions
Special
This group of warnings concerns conditions that can affect both your immediate personal safety
and the future safety of the instrument’s operators.
WARNING
Risk of personal or operator injury. Covers and interlocks are installed on Beckman
Coulter instruments to prevent injury from operating components. If you must
remove covers or disable interlocks to service an instrument:
1. Be alert and use extreme care when working around exposed components to
avoid personal injury.
2. At the end of the service call, always reinstall ALL instrument covers and
ensure interlocks are enabled to prevent operator injury.
WARNING
Risk of personal or operator injury. Broken or cut components with sharp edges
could puncture your gloves and skin, causing possible injury or biohazardous
contamination. Handle fragile components carefully to avoid breakage. Replace
any broken or cut components with sharp edges, even if they are still functioning.
Use mechanical means, such as hemostats, to handle a broken component,
whenever feasible. Dispose of broken components in accordance with the local
regulations and acceptable laboratory practice.
WARNING
Risk of personal or operator injury. Instrument doors, covers, and panels that are
mishandled can fail, leading to personal injury. Handle the doors, covers, and
panels with care and always follow the written instructions for opening and
closing or removing and reinstalling them.
WARNING
Risk of personal injury, operator injury, or damage to instrument components.
When performing a service call, always follow all applicable service manual or
Service Mod instructions. When questions arise, consult with Technical Support
for guidance.
B44425AC 1-7
Introduction
Electronic
WARNING
Risk of personal injury. Rings or jewelry can contact exposed electronic
components, causing personal injury from electric shock. Remove rings and other
metallic jewelry before performing maintenance or service on the electronic
components of the instrument.
CAUTION
Risk of damage to electronic components. Electrostatic discharge (ESD) can
damage add-in circuit cards and other electronic components. Perform
procedures with a possibility of ESD damage at an ESD review station, or wear an
antistatic wrist strap attached to a metal part of the chassis connected to an earth
ground. If removal/replacement of printed circuit card or components is
performed while the power is ON, damage to components may occur. To prevent
damage to electronic components, always ensure that the power is OFF before
removing or replacing printed circuit cards and components.
Biological
WARNING
Risk of personal injury and/or contamination. If you do not properly shield yourself
before servicing the instrument with the door open, you can be injured or
contaminated. To prevent possible injury or contamination, always wear gloves, a
lab coat, and eye protection when servicing the instrument with the doors open
and/or when working with pathogenic materials.
WARNING
Risk of biohazardous contamination. Biohazardous contamination could occur
from contact with the waste container and its associated tube if not handled with
care. Check the tubing connection and container location periodically. Wear
personal protective equipment. Avoid skin contact. Clean up spills immediately.
Dispose of the contents of the waste container in accordance with your local
regulations and good laboratory practices.
Use universal precautions when working with pathogenic materials. Means must be available to
decontaminate the instrument and to dispose of biohazardous waste.
1-8 B44425AC
Introduction
Troubleshooting
Bring the following warning to the customer's attention before advising that customer to perform
any service, maintenance, or troubleshooting procedures on the instrument.
WARNING
Risk of personal injury and/or contamination. If you do not properly shield yourself
before servicing the instrument with the door open, you can be injured or
contaminated. To prevent possible injury or contamination, always wear gloves, a
lab coat, and eye protection when servicing the instrument with the doors open
and/or when working with pathogenic materials.
Operational Hazards
Safety symbols alert you to potentially dangerous conditions.
The symbol applies to specific procedures and appears as needed throughout this manual.
Symbol Warning Condition Action

Biohazard Use universal precautions when working with pathogenic
materials. Means must be available to decontaminate the
instrument and to dispose of biohazardous waste.
Caution/Warning See the Safety Notice in this manual for more information.
Hot Surface Hot surfaces in this area. Avoid contact with any surface in
this area until you are sure that it has cooled down first.
Pinch Point Potential pinch or pierce point in this area. Be aware of the
moving probe and carefully present the test sample to avoid
injury.
B44425AC 1-9
Introduction
Hazard Labels
Carefully read the hazard warning labels on the instrument. The hazard labels are located on the
instruments as indicated below.
Disposal of Electrical Instrumentation

It is very important that customers understand and follow all laws regarding the safe and proper
disposal of electrical instrumentation.
The symbol of a crossed-out wheeled bin on the product is required in accordance with the Waste
Electrical and Electronic Equipment (WEEE) Directive of the European Union. The presence of this
marking on the product indicates:
1. The device was put on the European Market after August 13, 2005 and
2. The device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.
For products under the requirement of the WEEE directive, please contact your dealer or local
Beckman Coulter office for the proper decontamination information and take back program which
will facilitate the proper collection, treatment, recovery, recycling and safe disposal of device.
1-10 B44425AC
Introduction
Waste Disposal Warning

Be sure to dispose of waste in accordance with environmental protection regulations.
WARNING
Risk of biohazardous contamination. Biohazardous contamination could occur
from contact with the waste container and its associated tube if not handled with
care. Check the tubing connection and container location periodically. Wear
personal protective equipment. Avoid skin contact. Clean up spills immediately.
Dispose of the contents of the waste container in accordance with your local
The maximum waste line length is 1.50 m (5 ft). The waste drain tube supplied with the system can
be connected to either:
• An open drain, suitable for biohazardous waste, less than 76 cm (30 in.) above the floor
• A waste container with a minimum capacity of 2,000 mL (0.53 gal). Verify container is clearly
labeled and dispose of the contents of the waste container in accordance with your local
WARNING
When using an open drain instead of a waste container, mechanically secure the
waste tube into the drain, so that the tube cannot accidentally come out of the
drain. This prevents spillage.
CE Mark
A “CE”mark indicates that a product has been assessed before being placed on the market, and has
been found to meet European Union safety, health, and/or environmental protection requirements.
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
B44425AC 1-11
Introduction
China RoHS Caution Label
This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
China RoHS Environmental Label
This label indicates that the electronic information does not contain any toxic or hazardous substances. The
center “e” indicates the product is environmentally safe and does not have an Environmentally Friendly Use
Period (EFUP) date. Therefore, it can safely be used indefinitely. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
California Proposition 65
WARNING
This product may contain chemicals known to the State of California to cause
cancer and birth defects or other reproductive harm.
1-12 B44425AC
CHAPTER 2
Instrument Description
2.1 Introduction to the DxH 500 and DxH 520 Analyzers
Summary
The DxH 500 Series instruments are quantitative, multi-parameter, automated hematology
analyzers for in vitro diagnostic use in clinical laboratories; including hospital, reference, and
physician's office laboratories. The DxH 500 Series instruments are used to identify the normal
patient with normal system-generated parameters from patients with abnormal parameters
and/or flags that require additional studies. The integrated color display with graphical icon based
user interface is intended to facilitate ease of use and operator training. The DxH 500 Series
instruments utilize fully featured integrated software usually found on larger instrumentation.
The DxH 500 Series Systems

The DxH 500 Series instrument systems are comprised of the analyzer, software, and a group of
analytical and quality control reagents that allow for simultaneous quantitative determination of
hematological measurands through the use of impedance, spectrophotometry, and optical
technology. Additional reagents provide system cleaning, quality control and calibration. The
Diluent and Lyse reagents pass through a heating block prior to being used for the dilution of the
specimen. The heater maintains the reagents within a narrow temperature range to optimize their
reaction.
B44425AC 2-1
Components (DxH 500)

The major components of the DxH 500 system are indicated below.
Components (DxH 520)

The major components of the DxH 520 system are indicated below.
Shear Valve Module
Solenoid Valves
Assembly
Syringe
Heater Assembly
Assembly
Sampling
Assembly
Rinse Pump
Front Cover Assembly

Sampling
Drain Valves Assembly
Tube Holder Module and Drain Manifold
Counting
Assembly
2-2 B44425AC
2.1 Introduction to the DxH 500 and DxH 520 Analyzers 2
Accessories
• Printer
• Handheld 2D bar-code scanner
• External QWERTY Keyboard (optional)
Software
The DxH 500 Series Software is contained in the integrated computer that is located behind the built
in color touch screen display. All interactions with the software are via the color touch screen. A
handheld USB bar-code scanner is supported for input of specimen IDs and
reagent/controls/calibrator information.
The User Interface software is divided into main functional areas that are accessed via nine icons
on the left of the screen, and three icons on the top right of screen. The Software provides alarms,
prompts and messages to inform users of the system status. Software functionality includes:
• Back: moves to the previous screen.
• Display/Run: provides functionality for the identification of specimens and analysis of
specimens.
• Patient Results: provides functionality for the storage, review and reporting of results from
previously analyzed specimens.
• Worklist: provides functions to allow patient specific information (demographics) to be entered
and assigned to specimen results.
• Daily Checks: removes cleaner from system, confirms the system status and performs reagent
background check on a daily basis prior to system operation.
• QC/ QA: provides functionality for the quality control of the system performance through
analysis, storage and review of quality control specimens; provides functionality for the use of
patient specimens in assessment of instrument performance; provides functions for the
calibration of the instrument; provides function for the assessment of system repeatability and
carryover.
• Supplies: provides functions for the setup and tracking of reagent supplies used on the system.
• Logs: provides functionality for the tracking and review of system events and messages related
to Error, Warnings and Traceability.
• Setup: provides functionality to the user to configure the software and specific system
functions to suit the user needs, e.g. definition of reference ranges, configuration of reporting
options.
• Logout: controls user access and tracks the functions performed by the user.
• Functions: provides direct access to printing, transmitting and exporting functions applicable
to the screen displayed.
• Diagnostics: provides the user with functions used in the support, maintenance and cleaning of
the system.
The software provides the instrument control cycles used in the analytical process and
measurement of the specimens. It is responsible for the data collection and subsequent analysis of
the raw data collected during the analysis and the generation of the analytical results. The software
B44425AC 2-3
architecture is structured such that data is partitioned which allows for software updates to occur
without the loss of customer data.
[Software v2.0 and future releases] Backup and restore capabilities for data and system
configuration settings are available. All data and system settings are maintained when the system
is powered off.
Analytical Reagents
All reagents are barcoded for reagent management functions.
• DxH 500 Series Lyse - a cyanide-free, aldehyde-free erythrocyte lytic agent for the quantitative
determination of hemoglobin, classification of WBC subpopulations, and counting leukocytes.
• DxH 500 Series Diluent - an aldehyde-free, isotonic-buffered saline solution that is used to
dilute the specimen and used to rinse module components between sample analyses.
• DxH 500 Series Cleaner - a cyanide-free, aldehyde-free cleaning agent for components that
come in contact with blood samples to remove residual material from the system.
Functional Description
The DxH 500 Series instruments provide complete blood counts (CBC with five part differential
(5pd)). Blood specimens are processed by the DxH 500 using an open vial sampling method, while
for the DxH 520, blood specimens are processed by using either a closed-vial or open vial sampling
method.
Specimen Preparation (DxH 500)

1. The user presents an open vial of blood to the device sampling probe and initiates the cycle by
pressing the Start paddle. The aspiration syringe activates and aspirates 12 µL of sample into
the sample probe.
• Pre-Aspiration Syringe Vacuum Check:
Before sample aspiration, the vacuum level is checked. Limits = -700 to -300 mBar
• Post-Aspiration Syringe Vacuum Check:
After sample aspiration, the vacuum level is checked. Limits = -700 to -300 mBar
2. The sample probe retracts into the device. As the probe retracts, the external surfaces are
rinsed with DxH 500 Diluent to remove excess blood and dried.
• Pre-Probe Rinse Vacuum Check:
Limits: Vacuum differential >10% (See 2.3, Diluter System, Rinsing Head, DxH 500)
• Post-Probe Rinse Vacuum Check:
Limits: Vacuum differential >20% (See 2.3, Diluter System, Rinsing Head, DxH 500)
3. The sample probe moves above the White Blood Cell (WBC) bath. The system performs three (3)
Hgb blank measurements and the sample probe external surface is rinsed again.
4. The WBC bath is drained to waste and 1.0mL of Diluent is dispensed into the clean and empty
WBC bath via bath diluent input fitting. 0.5 mL of diluent is dispensed via the sample probe
pushing the 12 µL of sample into the bath and creating the initial WBC dilution of 1:125
(Blood : Diluent).
2-4 B44425AC
5. The initial WBC dilution is mixed using air bubbles to ensure homogeneous distribution of the
sample.
6. The sample probe aspirates 25 µL of the initial WBC dilution to be used for the RBC dilution. As
the sample probe retracts the external surfaces are rinsed.
7. The sample probe moves over the RBC bath. As the sample probe moves above the Red Blood
Cell (RBC) bath, 0.66 mL of Lyse is dispensed into the WBC bath to create the final WBC dilution
of 1:180. The dilution is air mixed in preparation for analysis. The Lyse destroys the RBC
membranes to release the contained hemoglobin while maintaining the WBCs. The WBC
dilution is used for the counting and differentiation of the WBCs and measurement of
hemoglobin.
8. 1.5 mL of Diluent is dispensed into the clean and empty RBC bath via the outside of the sample
probe. A further 0.5 mL of Diluent is dispensed via the sample probe pushing the 25 µL of the
initial WBC dilution into the RBC bath creating the final RBC dilution 1:10125. The RBC dilution
is mixed and prepared for the counting and sizing of the RBCs and PLTs.
Specimen Preparation (DxH 520)

1. The user presents a closed vial of blood to the tube holder and presses the Run button on the
Sample Analysis - Patient Run screen. The tube holder door automatically closes and initiates the
cycle.
2. The probe pierces the cap for venting then retracts into the device. As the probe retracts, the
external surfaces are rinsed with DxH 500 Series Diluent to remove excess blood.
3. The system performs three (3) Hgb blank measurements.
4. The probe pierces a second time and aspirates 16.7 µL of sample into the sample probe.
• Pre-Aspiration Syringe Vacuum Check:
Before sample aspiration, the vacuum level is checked. Limits = -700 to -300 mBar
• Post-Aspiration Syringe Vacuum Check:
After sample aspiration, the vacuum level is checked. Limits = -700 to -300 mBar
5. The sample probe retracts into the device again and the external surfaces are once again rinsed
with DxH 500 Series Diluent to remove excess blood.
6. The sample probe moves above the White Blood Cell (WBC) bath where 2.7 µL of blood is
ejected. The sample probe external surface is rinsed again.
7. The WBC bath is drained to waste and 1.25mL of DxH 500 Series Diluent is dispensed into the
clean and empty WBC bath via the bath diluent input fitting. A further 0.5mL of DxH 500 Series
Diluent is dispensed through the sample probe, pushing the 14 µL of sample into the bath and
creating the initial WBC dilution of 1:125 (Blood : Diluent).
8. The initial WBC dilution is mixed using air bubbles to ensure homogeneous distribution of the
sample.
9. The sample probe enters the WBC dilution and aspirates 306 µL of the initial WBC dilution; this
volume travels through the probe fluidic path and reach the shear valve. This valve segments
25 µL of the initial WBC dilution to be used for the RBC dilution.
10. Meanwhile 0.66 mL of DxH 500 Series Lyse is dispensed into the WBC bath to create the final
WBC dilution of 1:182. The dilution is air mixed in preparation for analysis. The DxH 500 Series
B44425AC 2-5
Lyse destroys the RBC membranes to release the contained hemoglobin while maintaining the
WBCs and measurement of hemoglobin.
11. The shear valve changes state to segment the dilution and 2.0 mL of DxH 500 Series Diluent is
dispensed into the clean and empty RBC bath, pushing the 25 µL of the initial WBC dilution into
the RBC bath thus creating the final RBC dilution 1:10125. The RBC dilution is mixed and
prepared for the counting and sizing of the RBCs and PLTs.
12. After counting, both baths are drained and rinsed, the probe is rinsed and the instrument is
ready for the next specimen.
Counting/Sizing/Optical Measurements
The RBC and WBC dilution are analyzed to generate the raw data used to determine the final output.
• RBC, WBC and PLT cell counts and sizes are determined using the Coulter Principle (impedance)
• The WBC 5 part differential is determined using a combination of the impedance WBC data and
the direct optical measurement (Axial Light Loss - ALL) using a blue LED focused through the
WBC aperture
• Three data acquisition channels (RBC and PLT Resistive, WBC Resistive and WBC ALL) are used
to collect and digitize the pulse information
Spectrophotometry Measurements
In the WBC bath with the addition of the Lyse, the RBCs are lysed and the released hemoglobin is
converted into stable Oxyhemoglobin (or Carboxyhemoglobin, if present). The resulting complex is
then measured by spectrophotometry using an LED.
User Interface and Device Interaction

• User interface functions are performed via an 8.4 inch built-in color touch screen. The touch
screen on the DxH 500 Series instrument is an analog resistive touchscreen. Analog resistive
touchscreen technology senses input from contact with essentially any object (finger,
stylus/pen, palm, etc.). The touchscreen responds to pressure on its surface so contact can be
made with gloved fingers or any pointing device.
• All software functions are accessible without the need for an external keyboard; however an
optional external USB PC keyboard is supported.
• The user interface software is divided into main functional areas that are accessed via nine
icons on the left of the screen, and three icons on the top right of screen (see examples in the
'detailed technical description' section below). This allows access to all major system functions
within one key (screen touch) from any screen. All sub-functions are accessible within three key
(screen touches) from any screen.
• The software provides alarms, prompts and messages to inform the user of the system status.
• A handheld USB bar-code scanner is supported for input of specimen identifiers (IDs) and
reagent/controls/calibrator information.
2-6 B44425AC
WARNING
To avoid being pierced by the aspiration probe, use caution when performing
procedures involving the probe.
• To analyze a blood specimen on the DxH 500, the user immerses the sample probe into the
specimen tube and presses the aspirate plate to initiate the cycle. To analyze a blood specimen
on the DxH 520, the user presents a closed vial of blood to the tube holder and presses the Run
button on the Sample Analysis - Patient Run screen. The tube holder door automatically closes
and initiates the cycle.
• Results generated by the DxH 500/520 are available to the user on the inbuilt screen, or can be
printed as hard copies and/or can be sent electronically (via ASTM compliant protocols using
serial RS-232 or Ethernet connections) to a Laboratory Information System (LIS).
B44425AC 2-7
2-8 B44425AC
2.2 Principles of Operation 2
2.2 Principles of Operation
Summary
The DxH 500 Series instrument uses a number of technologies for the analysis of the blood
specimens that include:
• Spectrophotometry for Hemoglobin measurement.

• Impedance measurements for the White Blood Cell, Red Blood Cell and Platelet counting and
sizing.
• Combined Optical and Impedance measurements to determine the WBC differential
distribution.
Spectrophotometry
Using the DxH 500 Series hemoglobin reagent (DxH 500 Series Lyse), the hemoglobin derivatives are
stabilized in an oxyhemoglobin ferrous form. This technique was chosen in order to measure
effective total hemoglobin and not underestimate carboxyhemoglobin in case of carbon monoxide
(CO) intoxications. The resulting complex is then measured by spectrophotometry using a LED.
Using the broad band width, the entire absorbance peak is essentially integrated.
Impedance Measurements
The Coulter Principle (impedance) is used to count and size cells by detecting and measuring
changes in electrical resistance when a particle (such as a cell) in a conductive liquid passes through
a small aperture. Each cell is suspended in a conductive liquid (diluent). As each cell goes through
B44425AC 2-9
the aperture, it momentarily increases the resistance of the electrical path between the submerged
electrodes on either side of the aperture. This causes a measurable electronic pulse. For counting,
vacuum is used to pull the diluted suspension of cells through the aperture at a regulated volume.
While the number of pulses indicates particle count, the size of the electrical pulse is proportional
to the cell volume.
The DxH 500 Series instrument uses the WBC and RBC bath assembly for RBC Count, MCV, PLT
Count, MPV and WBC Count.
Coincidence Corrections
Occasionally, more than one cell passes through the aperture at a time. When this occurs, only one
combined pulse is counted. As the frequency of the coincidence is proportional to the actual count,
the coincidence can be mathematically predicted and allows automatic correction of the raw data.
2-10 B44425AC
Voting and Averaging

To minimize data errors due to statistical outliers or obstructions that may block or restrict the flow
through an aperture for the WBC, RBC, Mean Cell Volume (MCV), Red cell Distribution Width
(RDW), Platelet (PLT) and Mean Platelet Volume (MPV), the system verifies that the data produced
is within an established statistical range (voting). If within defined limits, the data is used to
generate measurand results. If not within limits, the data is inhibited.
RBC and PLT Histograms

In addition to being counted, red blood cells (RBCs) are categorized according to size (volume)
categories to develop a size distribution curve of the RBC's. Platelet counting and sizing are also
done in the RBC bath. Thresholds separate the platelet pulses, which are much smaller from the red
blood cell pulses. Platelets are also categorized according to size to develop a size distribution curve
of the particles. Examples of RBC and PLT histograms are shown below.
WBC Optical and Impedance Measurements

The WBC differential (5 Part DIFF) is determined using the simultaneous measurement of
impedance (volume) and direct optical (Axial Light Loss) measurement within the WBC aperture.
B44425AC 2-11
The technology uses an elongated square aperture in the WBC bath in which the WBC dilution is
prepared and the analysis performed. The elongation of the aperture allows an optical assembly to
be placed perpendicular to the aperture. The optical assembly projects a blue LED light through the
aperture wall and onto a sensor (Axial Light Loss). As cells pass through the aperture, the optical
path is interrupted (blocked). The amount of light falling on a sensor can be measured and varies
depending on cell structure.
The technology does not employ the "traditional" flow cell with sample stream sheathing to ensure
cells are correctly aligned within the optical path - digital pulse processing is used to identify those
cells that pass axially through the aperture.
As depicted in the diagram below, cells that pass through the center of the aperture produce
Gaussian shaped pulses in the resistive measurement process; conversely those that are not
centered will produce non-Gaussian shaped pulses (T4).
2-12 B44425AC
The pulses generated are digitized allowing them to be analyzed for shape. If the shape of the
impedance pulse is determined to be Gaussian (T1, T2, and T3), the cell will have passed through the
center of the aperture and in doing so, it will be correctly positioned in the aperture for the optical
measurement. For impedance pulses that are determined to be Gaussian, the resistive pulse
information and the axial light loss optical measurement for the same cell are used in the analysis
to generate the WBC differential scatterplot. By plotting the impedance data against the optical
data, a two dimensional scatterplot is created that is used to differentiate the WBCs into the 5 major
WBC classifications (Lymphocytes, Monocytes, Neutrophils, Eosinophils and Basophils). Any
impedance pulse that is determined to be non-Gaussian is discarded along with the optical pulse and
is not used in the WBC differential analysis.
An example of a WBC Scatterplot with a normal WBC distribution (and the location of the different
WBC sub-populations) is shown below.
B44425AC 2-13
Differential Measurement
For WBC 5-part differential using 22ECO new technology (patent pending), direct optical
measurement in the aperture is also used for resistive measurement.
2-14 B44425AC
The main advantages are:
• Use of LED as optical source

• Use of only one dilution for HGB, WBC count and WBC 5-Part differential
• No tubing between bath and optical module
• No sheath and dedicated sampling syringe
• Reduced dilution volume.
Data Acquisition
In a standard flow cytometry medical device, the cells are kept centered in the reliable optical zone
by a sheath.
The technology uses the well known resistive measurement artifact to reject the cells not flowing
through the reliable optical measurement zone.
The data acquisition is numerically done using a FPGA three acquisition lines:
• RBC/PLT Res / WBC Res / WBC All
The data is sent to the Q7 single board computer for treatment.
B44425AC 2-15
Measurands and their Derivation
Table 2.2-1 Measureands
Measurand Method Description

WBC Coulter Principle (WBC White Blood Cell Count or Leukocyte Count
Bath) • Measured directly in WBC bath.
• WBC = N x 103 cells/μL
RBC Coulter Principle (RBC Bath) Red Blood Cell Count or Erythrocyte Count
• Measured directly in RBC bath.
• RBC = N x 106 cells/μL
Hgb Spectrophotometric Hemoglobin or Hemoglobin Concentration
Measurement (WBC Bath) • Transmittance of light at 545 nm through a lysed WBC
solution in the WBC bath, compared to the
transmittance of the same light through a reagent
blank.
• Hgb (g/dL)
Hct Calculated Hematocrit
• The relative volume of packed erythrocytes to whole
blood
• Hct (%) = (RBC X MCV)/10
MCV Derived from RBC Mean Corpuscular Volume
Histogram • The average volume of individual erythrocytes derived
from the RBC histogram
• Expressed in fL
MCH Calculated Mean Corpuscular Hemoglobin
• The weight of Hgb in the average erythrocyte
• MCH (pg) = (Hgb/RBC) x 10
MCHC Calculated Mean Corpuscular Hemoglobin Concentration
• The average weight of Hgb in a measured dilution
• MCHC (g/dL) = (Hgb/Hct) x 100
RDW Derived from RBC Red Cell Distribution Width
Histogram • The size distribution spread of the erythrocyte
population derived from the RBC histogram.
• Expressed as coefficient of variation (%)
RDW-SD Derived from RBC Red Cell Distribution Width - SD
Histogram • The size distribution spread of the erythrocyte
population derived from the RBC histogram
• Expressed as a standard deviation in fL
PLT Coulter Principle (RBC Bath) Platelet Count or Thrombocyte Count
• The number of platelets derived from the Plt
histogram, measured in the RBC bath
• Plt = N x 103 μL
2-16 B44425AC
Table 2.2-1 Measureands
Measurand Method Description

MPV Derived from Plt Histogram Mean Platelet Volume
• The average volume of individual platelets derived
from the Plt histogram
• Expressed in fL
NE Optical / Impedance (WBC Neutrophil Percent
bath) • [NE events/(NE+LY+MO+EO+BA events)] x 100
• Expressed as a percentage (%)
LY Optical / Impedance (WBC Lymphocyte Percent
bath) • [LY events/(NE+LY+MO+EO+BA events)] x 100
MO Optical / Impedance (WBC Monocyte Percent
bath) • [MO events/(NE+LY+MO+EO+BA events)] x 100
EO Optical / Impedance (WBC Eosinophil Percent
bath) • [EO events/(NE+LY+MO+EO+BA events)] x 100
BA Optical / Impedance (WBC Basophil Percent
bath) • [BA events/(NE+LY+MO+EO+BA events)] x 100
NE# Calculated Neutrophil Absolute Count
• NE# (103/μL) = (NE/100) x WBC
LY# Calculated Lymphocyte Absolute Count
• LY# (103/μL) = (LY/100) x WBC
MO# Calculated Monocyte Absolute Count
• MO# (103/μL) = (MO/100) x WBC
EO# Calculated Eosinophil Absolute Count
• EO# (103/μL) = (EO/100) x WBC
BA# Calculated Basophil Absolute Count
• BA# (103/μL) = (BA/100) x WBC
B44425AC 2-17
2-18 B44425AC
2.3 Diluter System 2
2.3 Diluter System
Overview
When operating the DxH 500 Series system, the operator starts the cycle by pressing the Run button
on the Sample Analysis screen. Sample and reagent movement is achieved using multiple 2-way and
3-way solenoid valves, a multi syringe assembly composed of a sample syringe, a diluent syringe ,
two syringes for vacuum/pressure, and a lyse syringe. No pinch valves or diluent reservoirs are
used on the instrument.
DxH 500 Series systems use the Coulter Principle of impedance counting. The Coulter principle
states that particles pulled through an orifice, concurrent with an electric current, produce a
change in impedance that is proportional to the size of the particle traversing the orifice. The
Coulter principle was named for its inventor, Wallace H. Coulter.
Main Components
• Sampling Module/ Rocker Assembly (includes the sample probe)
• Counting Module (includes RBC bath and WBC bath/Optics Module)
• Solenoid Valve Module
• Syringe Assembly
• Tube Holder Module (DxH 520 only)
Solenoid
Valves
Sample Probe
Sampling Module
Syringe
Assembly
Tube Holder Module

(DxH 520 only)
Syringe
Motor
RBC Bath WBC Bath Optical Bench
B44425AC 2-19
2.3 Diluter System
The sampling module/rocker assembly acts as a transport system for the sample from the sample
vial to the baths for analyzing. The sample probe functions as an aspiration tip. This sampling
module/rocker assembly performs two functions:
1. Vertical movement for sample aspiration

2. Horizontal/rocker movement to transfer sample from specimen tube to the counting baths.
Movement of the sampling module is accomplished using two stepper motors. The motors move the
probe either vertically or horizontally as needed. An optical sensor on the vertical traverse
assembly controls the positioning of the probe for aspiration and dispense functions. Another
optical sensor controls the horizontal position of the probe to ensure sample is deposited in the
correct baths.
Probe Movements
Horizontal Probe Movement

Horizontal movement is achieved with the following components:
• Horizontal rocker stepper motor - moves the assembly from front to back
• Horizontal rocker optical sensor - identifies proper position of the assembly
Horizontal movement is by gear/teeth. The horizontal stepper motor controls probe positioning
over the aspiration station (home) position, over the WBC bath, and over the RBC bath.
2-20 B44425AC
The sampling / rocker assembly has three (3) horizontal positions:
1. aspirate position
2. over the WBC bath
3. over the RBC bath
Vertical Probe Movement

Vertical movement is achieved with the following components:
• Vertical probe stepper motor - moves the probe up and down

• Vertical probe optical sensor - identifies proper location of the probe
A belt drive system is employed for vertical motion. The DxH 500 and DxH 520 use one optical sensor
to control the vertical movement of the probe over the aspirate station and over the WBC and RBC
baths.
The probe has four (4) vertical positions:
1. Probe DOWN ready to sample (Probe DOWN)

2. Probe UP (Probe HOME)
3. Probe rinse position ( inside both WBC and RBC baths)
4. Sample dispense/thief position (dispensing of whole blood into WBC bath, aspiration for the
RBC dilution while in the WBC bath, dispensing of the RBC dilution into the RBC bath).
Sample Probe
The sample probe used on the DxH 500 has the opening on the bottom of the tip. The sample probe
used on the DxH 520 has an opening on the side of the probe, just above the tip.
Rinsing Head
When a function such as aspiration of the patient sample or of the WBC dilution is complete, the
probe moves up into the rinsing head where it is cleaned by a combination of diluent and vacuum.
The rinsing head performs the following task:
• Probe washing and probe drying

• Path for diluent into the RBC and WBC chambers
DxH 500
To clean and dry the probe after aspiration, the following occurs:
• The vacuum present after sample aspiration is measured. This is the Post-Aspiration Vacuum
Check (Reading A).
• VL3 is energized and the existing vacuum from the vacuum/pressure syringe is routed through
VL3 to the upper port of the rinsing head (via the larger diameter tubing).
B44425AC 2-21
2.3 Diluter System
• After approximately 200ms, the vacuum is measured again. This is the Pre-Probe Rinse Vacuum
Check (Reading B). If the vacuum difference between this reading and the last reading is less
than 10%, a Post-Probe Rinse Vacuum Error is generated.
[(Reading A - Reading B) > 10% of Reading A].
• VL4 is energized to open the pathway (across VL5, VL6, and VL12) from the diluent syringe to
the lower port on the rinsing head while the probe retracts.
• Once the probe is rinsed, the vacuum is measured again. This is the Post-Probe Rinse Vacuum
Check (Reading C). If the vacuum difference between this reading and the last reading is less
than 20%, a Post-Probe Rinse Vacuum Error is generated.
[(Reading B - Reading C) > 20% of Reading B].
To route diluent into the RBC and WBC chambers, vacuum to the upper port of the rinsing head is
closed thus allowing the diluent to flow down the sides of the probe.
DxH 520
To clean and dry the probe, VL4 and VL12 are energized to open the pathway (across VL5, VL6, VL12,
and VL3) from the diluent syringe to the lower port on the rinsing head. At the same time vacuum
is routed by the rinse pump to the upper port of the rinsing head (via the larger diameter tube). To
route diluent into the RBC and WBC chambers, vacuum to the upper port of the rinsing head is
closed thus allowing the diluent to flow down the sides of the probe.
Hydraulic / Pneumatic System

The DxH 500 Series instruments use a syringe assembly and solenoid valves to control the transfer
of—
• sample from sample vial to counting baths
• reagents from the reagent containers to the baths
• the removal of spent sample from the instrument to the waste container.
All components are modular in design and all tubing, with the exception of the sample probe tubing
and rinsing head tubing, is contained in the center compartment of the instrument. Replacement
of all components, except the sample probe and rinse head, can be done without the need to remove
tubing from fittings.
Main Modules
• Rocker module
• Counting module
• Drain/Mix Valve module
• Solenoid Valve module
• Syringe module
• Tube Holder Module (DxH 520 only)
• Shear Valve Module (DxH 520 only)
2-22 B44425AC
Rocker Module
• Sample probe
• Rinsing head
• Rocker motor and sensor
• Probe motor and sensor
• Probe belt
• Probe carriage
• Rocker
• Probe carriage axis
• Rocker guide
Counting Module
• RBC counting chamber
• WBC counting chamber/optics assembly
• Counting manifold
• Barbed fittings
• Chamber shield
• WBC bath filter (DxH 520 only)
Drain Valve Module

• Electro solenoid valves used for draining and mixing baths
• Valve manifold
• Barbed fittings
Electro Solenoid Valve Module

• Electro solenoid valves used for transfer of waste, reagents, pressure and vacuum
• Valve manifold
• Barbed fittings
NOTE Electronic solenoid valves:

• 2-way valve in the inactive/rest state are Normally Closed (NC) - NC= blocks flow; NO = allows flow.
• 3-way valve in the inactive /rest state are Normally Open (NO) port to common (I/O) port.
Syringe Assembly (Single Block Assembly)

• Syringe stepper motor
• Syringe optical sensor
• Sample syringe
• Diluent syringe
B44425AC 2-23
2.3 Diluter System
• Vacuum / pressure syringes (internally connected)

• Lyse syringe
Tube Holder Module

• Tube holder
• Tube holder housing
• Tube holder door
• Tube holder door motor
• Tube holder door sensors board
• Tube holder door sensor
Shear Valve Module

• Shear valve motor
• Shear valve holder
• Shear valve head
• Shear valve sensor
2-24 B44425AC
Reagent Transfer Cycles

This section contains information pertaining to reagent and waste flow on the DxH 500 and DxH 520
instruments. Unless otherwise indicated, the information applies to both instruments.
Diluent
Diluent is aspirated (down stroke) from the diluent container by the Diluent piston in the syringe
assembly via Normally Open port on Valve VL4. On the upstroke the diluent is routed through
Normally Closed port on VL4 then to the following valves depending on task:
1. DxH 500: VL5 -> VL6 -> VL12 -> to the Rinsing Head for probe cleaning
DxH 520: VL5 -> VL6 -> VL12 -> VL3 -> to the Rinsing Head for probe cleaning
2. VL5 -> VL6 -> Through the Heater -> to the WBC Bath for sample dilution and cleaning
3. DxH 500: VL5 -> VL6-> VL12-> to the rear of Apertures for Back flush and to replace cleaner with
diluent during the measurement phase.
DxH 520: VL5 -> VL6-> VL12-> VL3-> to the rear of Apertures for Back flush and to replace
cleaner with diluent during the measurement phase.
4. DxH 500: VL5 -> to the RBC and WBC Baths to push the sample from the probe and clean the
inside of the probe.
DxH 520: VL5 -> Shear Valve IN to the WBC Bath to push the sample from the probe and clean
the inside of the probe.
5. DxH 520: VL5 -> VL6 -> VL12 -> Shear Valve OUT to the RBC Bath for sample dilution and
cleaning.
Lyse
Lyse is aspirated from the Lyse container by the Lyse syringe (down stroke) via the NO port on VL9.
On the upstroke the Lyse is routed through the NC port on VL9, through the heater to the WBC bath.
Cleaner
The cleaner reagent does not have a dedicated syringe. Cleaner performs several functions; clean
the apertures between samples, clean the waste tubing and waste syringes, and to clean the baths
during shutdown.
To clean the apertures between samples VL10 and VL11 are energized. This allows the cleaner to be
pulled through the count lines and reach both the RBC and WBC apertures.
To clean the baths during shutdown the cleaner is pulled through VL10 into the vacuum/waste
syringes. VL10 closes and the cleaner is sent to the bottom on baths via VL2 (RBC drain) or VL1 (WBC
Drain).
Waste
Spent blood from the bath as well as blood from the rinsing head is removed from the instrument
by the vacuum/waste syringes. When VL1 and VL2 are energized vacuum from the vacuum/
pressure syringes empties the baths.
B44425AC 2-25
2.3 Diluter System
DxH 500: When VL3 is energized vacuum from the vacuum/pressure syringes draws the blood from
the rinsing head into the vacuum/waste syringe. Waste is routed to the waste container by moving
the vacuum/pressure syringes UP and energizing VL7.
DxH 520: Vacuum from the rinse pump draws the blood from the rinsing head and directs it to the
waste container.
Pneumatics System
The pneumatics/hydraulics system on the DxH 500 Series instruments are handled by the vacuum/
pressure/waste syringe pistons and a rinse pump (DxH 520 only).
The two large syringes in the syringe assembly are responsible for the generation of both vacuum
and pressure. These syringes are internally connected and act as a source for pressure/vacuum. On
the DxH 520, the rinse pump acts a source for vacuum during probe cleaning.
The level of pressure and vacuum generated by the vacuum/ pressure/ waste syringe pistons is
monitored by a pressure/vacuum transducer located in the mainboard. This transducer is directly
connected to one of the ports in the vacuum/pressure/waste syringe manifold. By monitoring
expected levels of pressure and/or vacuum during specific times within the instrument cycles, the
instrument is able to check activities such as counting, draining of baths, presence of reagent in
baths, probe cleaning (DxH 500 only), and valve performance.
The level of vacuum generated by the rinse pump on the DxH 520 is monitored by an inline pressure
sensor located in the rinse pump pathway and connected to the mainboard.
Vacuum (Negative Pressure from Pressure/Vacuum Syringes)

In order to create vacuum (negative pressure), starting from syringe home position (UP) the
stepper motors move the syringe pistons down creating the vacuum. Depending on the task,
different valves will open and close to properly route the vacuum.
NOTE Since the volume of the pressure/vacuum syringes is much greater that any of the other syringes, it
is possible for the vacuum/pressure syringes to be moving UP and still have sufficient vacuum to perform
specific tasks.
• Drain Baths- Vacuum from the vacuum/pressure syringe is routed to the drain valves VL 1 or VL2.
Depending which bath needs to be drained, VL1 (WBC) or VL2 (RBC) will be energized to allow the
vacuum to drain the baths.
• Clean Probe (DxH 500 only)- Vacuum from the vacuum/pressure syringe is routed to rinsing valve
VL3. VL3 is energized to allow vacuum to enter top port on the rinsing head.
• Count sample- Vacuum from the vacuum/pressure syringe is routed to count valve VL10. VL10 is
energized to allow the vacuum to reach the RBC aperture. By design, the RBC and WBC apertures
are connected therefore the same vacuum used for RBC count is routed to the WBC aperture.
Pressure
In order to create pressure, starting from syringe home position (UP), VL8 is energized (Opened)
creating an open path to atmosphere, and the stepper motors move the syringe pistons down filling
2-26 B44425AC
them with air. When they reach the down position, VL8 is de-energized (closed) and the stepper
motor starts moving the syringe pistons up thus creating pressure inside the syringe.
NOTE VL8 is used similarly to expel air and create vacuum.

• Mixing bubbles- Pressure from the vacuum/pressure syringe is routed to the drain valves VL1 or
VL2. Depending which chamber needs mixing, VL1 (WBC) or VL2 (RBC) will be energized to allow the
pressure to enter the isolation section of the bath creating the mixing bubbles.
Count
The DxH 500 Series instruments use 6.8 inHg (-230mb) vacuum for counting. Vacuum generated by
the vacuum/pressure syringe is routed through VL10 to RBC aperture. By design a portion of the
vacuum is used to pull cells through the RBC aperture. The rest of the vacuum is routed from the
RBC bath to the WBC aperture to pull cells through the WBC aperture.
The DxH 500 Series instruments use a Rinse Flow design to remove red blood cells from behind the
RBC aperture. Between each count cycle clean diluent is pushed through the back of the apertures
and through the line connecting the RBC and WBC baths. During the count cycle, the clean diluent
which is pulled from the WBC bath to the RBC bath flows behind the RBC aperture and "rinses" away
cells to prevent double counting.
Aperture Sensing System

The aperture sensor system responsible for sample analysis consists of a RBC bath and WBC bath/
optical bench, each with an aperture assembly for cell count and size.
These components are mounted on a plate that is electrically isolated from the chassis.
The RBC counting chamber is a single component which incorporates the following:
• Counting chamber
• RBC aperture
It can only be replaced as a single piece. The RBC aperture is sandwiched between the chamber and
the counting block and sealed by two "O" rings. The aperture is round in shape, 50micron Diameter
x 92 micron length.
The RBC chamber is connected to the instrument via the internal electrode coaxial cable and the
external electrode stainless-steel fitting.
The WBC bath/optics assembly is a single component which incorporates the following:
• Counting chamber
• WBC aperture
• Diff LED
• Collimator assembly
• HGB LED
• HGB pre amplifier board
• ALL (Axial Light Loss) sensor board
B44425AC 2-27
2.3 Diluter System
• WBC bath filter (DxH 520 only)
The WBC aperture is sandwiched between the chamber and the counting block and sealed by two
O-rings. The aperture is square in shape, 80x80 micron x 90 micron length.
Due to the critical alignments involved in the manufacturing process, this WBC bath /optics
assembly cannot be adjusted in any way and must be replaced as a single piece if any of the above
listed components is suspect.
HGB Parameter
The DxH 500 and DxH 520 use the Oxyhemoglobin ferrous form of HGB measurement.
The HGB LED emits a beam of monochromatic light with a central wavelength of 545 nm. This light
passes through the sample inside the WBC chamber to the photodiode located on the Hgb board
mounted on the opposite side. This photodiode senses the light and converts the light into a voltage
which is then amplified.
To obtain a HGB parameter result, a Hgb Blank voltage and a Hgb Read voltage must first be
determined.
• HGB Blank voltage- three (3) readings are taken 7.1 seconds after the start of a cycle. These three
readings are taken at 0.3-second intervals while diluent is in the WBC bath.
• HGB Read voltage 1st counting Period- three (3) readings are taken 36.1 seconds after the start
of a cycle. These readings are taken at 1-second intervals while lysed WBC dilution is in the WBC
bath.
• HGB Read voltage 2nd counting Period- seven (7) readings are taken 41. 8 seconds after the start
of a cycle. These readings are taken at 1-second intervals.
• The HGB Read voltages are then compared to the HGB Blank reference voltages to determine
the final result displayed for the HGB parameter.
2-28 B44425AC
2.4 Electronics 2
2.4 Electronics
This section provides information on the following components:
• Power supply
• Mainboard
• Interface board
Power Supply
The DxH 500 and DxH 520 instruments have an external switching power supply rated at 50/60 Hz
with a voltage range of 100 to 264 Vac. The Electronic Power Supply is connected to the instrument
via a connector in the rear of the instrument.
The Electronic Power Supply supplies 24 VDC to two circuit boards; the Interface board located in
the rear of the instrument and the Mainboard located in the front of the instrument behind the
display.
Mainboard
The Mainboard performs the following—
• Controls and monitors all functions of the system
• Receives input from RBC and WBC/HGB/Optical bench
• Processes signals, performs A/D conversions, calculates and provided output (results)
• Stores (flash memory) gain values, calibration factors and reference values. (This is done during
every Shutdown.)
• Drives all valves, motors, and pumps
• Monitors ambient and reagent temperature
• Monitors vacuum
• Monitors system power supply
• Monitors all optical sensors
• Stores mechanical adjustment values (probe adjustments).
B44425AC 2-29
2.4 Electronics
The removable solid state Q7 board:
• contains the software

• stores Gain values
• stores Calibration factors.
Nine external electrical connectors are provided, one for the power supply, RS232, one for ASTM
host communication, and five USB connectors (one in the front, four in the rear).
Connectors and Cables

All components of the DxH 500 and DxH 520 are controlled by the Mainboard. The Mainboard
provides the connections for the cables to and from the various components. For Mainboard
connector layout, refer to Removal of Front Cover. For the specific description and part number of
these cables, refer to Cables in the publication, DxH 500/520 Parts Lists, PN B93296.
Battery
The battery is a manganese dioxide lithium battery (CR-2032, 3V coin battery) located on the
Mainboard. The Mainboard is shipped with the battery installed.
2-30 B44425AC
2.4 Electronics 2
Interface Board
The Interface board provides eight external electrical connectors—
• one serial LIS port for ASTM host communication

• four USB connectors for connecting the keyboard, handheld bar-code scanner, printer
• one Ethernet connector
• one "D" USB connector
• power supply connector.
There is also an additional USB connector in the front of the instrument. The Interface board can
support USB 2.0 compatible, full speed.
B44425AC 2-31
2.4 Electronics
2-32 B44425AC
CHAPTER 3
Installation Procedures
3.1 Pre-Installation Checks
You Need
• Three hours for full install including verification

• Four feet of cleared counter space for the instrument and printer
• A location for the instrument within six feet of an electrical outlet
• An area free of electromagnetic interferences and combustible materials
NOTE DxH instruments are supplied with a US power cord. If it is not suitable for the customer site, you
must obtain the correct power cord.
Electrical Requirements
CAUTION
Risk of erroneous results. Do not use an extension cord to connect the instrument
to a power outlet. Using an extension cord can increase electrical interference
that could affect the instrument's results. Place the instrument close enough to a
power outlet so that an extension cord is not necessary.
This instrument requires the following:
• 100-240 Vac
• 50-60 Hz
• Single phase with ground
• The ground is a confirmed third-wire earth ground that can carry the full current of the circuit
• The circuit is independent and protected
• External power supply (supplied with the instrument) as:
— Output Voltage: 24 V
— Current: 6.25A
B44425AC 3-1
• To reduce the risk of electrical shock, this device uses a three-wire electrical cable and plug to
connect (ground) the equipment to earth. When replacing the cord, use equivalent ratings as
follows:
— U.S.A. cord:
· Plug Type: 498G, 15A, 125V
· Connector Type: C13, 10A, 250V
· Cord Set Rating: 10A, 125V
— European cord:
· Plug Type: VIIG, 16A, 250V
· Connector Type: C13, 10A, 250V
· Cord Set Rating: 10A, 250V
DO NOT use a three-to-two wire plug adapter.
Power Consumption
Less than 120W
Carton Arrival and Condition

The DxH 500/520 system is tested before it is shipped from the factory. International symbols and
special-handling instructions tell the carrier how to handle these electronic instruments.
When you receive your instrument:

• Carefully inspect the carton.
• If you see signs of mishandling or damage, file a claim with the carrier immediately
• If the instrument is insured separately, file a claim with the insurance company
Operational Ambient Temperature, Humidity and Altitude

The instrument configured with DxH 500 Series consumables meets performance specifications
when operated at a working temperature of +18 to 32°C (64.4 to 89.6°F).
The instrument meets performance claims when operated at a maximum of 80% relative humidity
(non-condensing) at 32° C (89.6° F).
The instrument can operate at two different altitude ranges:
• Normal – Up to 1,500m (4,291 ft)

• High - 1,501m to 3,000m (4,925 ft to 9,843 ft)
The proper altitude range will be set up during the installation process.
3-2 B44425AC
3.1 Pre-Installation Checks 3
Space and Accessibility Requirements

Check the site for proper space allocation. In addition to the space required for the instrument itself,
arrange for:
• Comfortable working height

• At least 30.5 cm (12 in.) on each side for preferred access to perform troubleshooting procedures
and replacing reagents
• At least 10 cm (4.0 in.) behind the instrument for tubing, cabling and ventilation.
Drainage
• If the waste from the instrument will be ported into an open drain instead of a waste container,
make sure the drain is chemically resistant and is appropriate for biohazardous waste.
NOTE The length of the waste line supplied with instrument must not be altered (1.50 m / 5 ft)
• The drain or waste container must be located such that the top of the waste container is always
below the waste fitting on the back of the instrument as shown.
Installation Supplies
• Instrument reagents, calibrators, and controls must be available and within expiration dates
• The paper supplies and blood collection tubes must be available
• Tube specifications can be found at www.beckmancoulter.com
• Temp Admin Password
B44425AC 3-3
3-4 B44425AC
3.2 Unpack and Inspect the Instrument 3
3.2 Unpack and Inspect the Instrument
1 Carefully open the top of the box.
2 Firmly grasp the base of the instrument by inserting your hands into the box through the space
in front and in back of the instrument. Have the second person hold the box down as the
instrument is pulled out.
3 Lift instrument to the counter where it will be installed. Due to the packaging design the
packing material will come out with the instrument.
NOTE Instrument weighs less than 12 kg (26.4 lbs).
4 Remove the packing material, plastic bag protecting the instrument, and the plastic laminate
protecting the screen.
5 Open the left side reagent compartment door of the instrument and remove the pickup tube,
handheld scanner box, power supply and Accessory Box as shown below. Remove additional
packing material.
6 Open the Accessory Box and verify the contents against the Packing List.
Table 3.2-1 DxH 500/520 Accessories - Installation Parts
Quantity Description Part Number

1 DILUENT pickup tube RXH60084
1 WASTE pickup tube, container RXH60085
1 WASTE pickup tube, sink RXH60118
B44425AC 3-5
3.2 Unpack and Inspect the Instrument
Table 3.2-1 DxH 500/520 Accessories - Installation Parts
Quantity Description Part Number

1 160W switching power supply RXH60001
1 US Power line cord RXH60029
1 Screwdriver, slot 1/4" RXH60101
1 USB Handheld Bar-code Scanner B26599
1 Rinsing Head O-ring RXH60042
1 DxH 520 Rinsing Head Spacer (CP Only) RXH60102
1 Lubricant - Silicon grease (3gr) - Syringe Pistons RXH60042
1 Short Arm TORX T20 Tool RXH60099
7 Locate the serial number on the back of the instrument and verify that it matches the serial
number on your sales order.
3-6 B44425AC
3.3 Connecting Waste and Reagents 3
3.3 Connecting Waste and Reagents
Connecting Waste Line to Waste Container

You will need:
• Waste pickup tube for draining to a waste container, PN RXH60085

• Waste container
NOTE Waste container default size is 2,000 mL (0.53 gal).
WARNING
Risk of biohazard contamination. The waste container must be located in a safe
place on the floor or on a shelf lower than the instrument. The top of the waste
container should always be lower than the bench where the instrument is
installed. Verify tubing connection integrity periodically. Do not replace the waste
container while the instrument is cycling.
1 Place the waste container on the floor or on a shelf lower than the instrument.
NOTE Top of waste container must be lower than the waste connector in the back of the instrument.
2 Locate the waste container tube.
3 Locate the red fitting, marked WASTE, on the back of the instrument as shown.
4 Connect the waste tube onto the red fitting. Turn the red connector on the back of the
instrument firmly counterclockwise while holding the waste tube until secure.
B44425AC 3-7
5 Remove the cap from the waste container and set the cap aside for use when the container is
full.
6 Insert the waste pick-up tube into the waste container.
7 Turn the tube's cap onto the waste container until properly secured.
Connecting Waste Line to Sink

IMPORTANT When using an open drain instead of a waste container, mechanically secure the waste tube
into the drain, so that the tube cannot accidentally come out of the drain. This prevents spillage.
You will need:
• Waste line for draining into sink PN RXH60015
WARNING
Risk of biohazardous contamination. Use caution when draining the waste directly
into an open drain. Ensure that the waste line is mechanically secured into the
drain so the tubing cannot accidentally come out. If you are using this method of
waste removal, Beckman Coulter recommends that you schedule routine
maintenance of the laboratory drain pipes. The waste tubing length must not
exceed 1.50 m (5 ft).
1 Locate the sink waste tube.
2 Insert the sink's waste tube into sink and secure it with a tie wrap or other securing material.
Ensure that tube is not restricted by the tie wrap or other securing material.
3 Locate the red fitting marked WASTE, in the back of the instrument and connect the other end
of the waste tube as shown.
3-8 B44425AC
3.3 Connecting Waste and Reagents 3
B44425AC 3-9
3-10 B44425AC
3.4 Connecting the Reagents 3
3.4 Connecting the Reagents
CAUTION
Risk of erroneous results. Ensure that the reagent pickup tubes remain clean and
free of contamination. Avoid contact with the interior of the reagent container or
its contents, laboratory surfaces, or your gloved hands.
CAUTION
Risk of erroneous results or instrument damage. Place reagents on the same level
as the instrument. Do not place the reagent containers on top of the instrument
or below the instrument. Store and use the reagents as directed by the reagent’s
accompanying instructions. Note the expiration dates and open-container
stability days of all reagents. Do not use expired reagents. When you change
Beckman Coulter DxH 500 Series Diluent, Beckman Coulter DxH 500 Series Lyse or
Beckman Coulter DxH 500 Series Cleaner, be sure to prime the reagent and run a
background cycle to see if the results meet the background limits.
Connecting Diluent
1 Locate the Diluent line, PN RXH60084.
2 Open the Diluent container.
3 Holding the pick-up tube by the cap, insert the pick-up tube into the diluent container.
NOTE Be careful not to touch the pick-up tube to any surface to avoid possible contamination.
4 Turn the tube's cap onto the open Diluent container until properly secured.
B44425AC 3-11
5 Connect the other end of the diluent pick-up tube to the connector labeled DILUENT on the
back of the instrument by firmly pushing the diluent tube connector into the connector on the
back until it clicks as shown.
6 Diluent container must be placed at same level as the instrument.
Connecting Lyse Reagent
1 Open the left side reagent compartment door.
2 Locate the Lyse pick-up tube (identified by the Yellow sleeve near screw cap).
3 Open Lyse container.
4 Holding pick-up tube by the cap, insert Lyse pick-up tube into the Lyse container.
CAUTION
Be careful not to touch the pick-up line to any surface to avoid possible
contamination.
5 Tighten reagent pick-up cap onto the Lyse bottle.
3-12 B44425AC
3.4 Connecting the Reagents 3
6 Place reagent in Reagent compartment as shown.
Connecting Cleaner Reagent
1 Open the left side reagent compartment door.
2 Locate Cleaner pick-up tube (identified by the Blue tube near screw cap).
3 Open Cleaner container.
4 Holding pick-up tube by the cap, insert Cleaner pick-up tube into Cleaner container.
NOTE Be careful not to touch the pick-up line to any surface to avoid possible contamination.
5 Tighten reagent pick-up cap onto Cleaner bottle.
B44425AC 3-13
6 Place reagent in the Reagent compartment (as shown) and close door.
3-14 B44425AC
3.5 Connecting the Instrument's Electronic Components 3
3.5 Connecting the Instrument's Electronic Components
To connect the bar-code scanner, printer and external power supply, locate the appropriate
connectors at the rear panel as shown below.
Connecting the Bar-Code Scanner
1 Remove the bar-code scanner from the packaging.
2 Follow the instructions enclosed with the bar-code scanner to install the clamp-on ferrite core.
3 Connect the handheld bar-code scanner USB cable into any USB port on the rear of the
instrument.
Connecting the Printer

The system has pre-installed drivers for the optional USB printers available with this instrument.
Use the driver that applies to your printer, as applicable.
Ensure there is a USB printer cable available. If one is needed, PN B52612 may be ordered.
B44425AC 3-15
CAUTION
Verify printer operation by inspecting the printout that is available at every
startup:
• “Wireless printing capability (e-print) must be disabled by Service Rep at
installation”.
• “Printer can be only used in the USB configuration”. (Printer can ONLY be used
in a USB configuration and should not be connected to the Lab network.)
1 Follow the instructions provided with the printer for unpacking and setting up printer.
2 Connect the printer USB cable into any USB port on the rear of the instrument.
3 Connect printer to electrical outlet but Do Not turn power ON at this time.
4 Instructions for setting up the printer drivers can be found in the Printer Setup section of the
Installation Instructions.
Connecting the External Power Supply

NOTE DxH instruments are supplied with a US power cord. If it is not suitable for the customer site, you must
obtain the correct power cord.
1 Remove power supply from packaging and ensure the power cord supplied will work in your
lab.
2 Connect the non-pronged end of the power cord into AC INPUT on the external power supply.
3 Connect the small multiple pin connector to the back of the instrument.
NOTE Flat side of connector faces the Diluent/Waste fittings as shown below.
CAUTION
Risk of erroneous results. Ensure the external power supply and other electrical
components are not in contact with the diluent pickup tubing in the back of the
instrument.
3-16 B44425AC
3.5 Connecting the Instrument's Electronic Components 3
4 Connect the pronged end of the power cord directly into an electrical outlet.
B44425AC 3-17
3-18 B44425AC
3.6 Powering Up Instrument 3
3.6 Powering Up Instrument
1 Ensure that Waste and reagent lines are secured to the instrument and to the individual waste
container or sink containers.
2 Power instrument ON by pressing the ON/OFF button in the front panel. There is a 3-5 second
delay before the display comes up.
NOTE ON/OFF button serves to power the instrument ON and also as an indicator that the instrument is
active (Glows RED) or inactive/finished cycle (Glows GREEN). It also acts as an emergency stop
button.
3 Instrument to perform a system initiation cycle.
4 When the Log In screen appears, enter the Service Login credentials and select a language from
the drop-down menu.
5 Press the right arrow next to Password field to continue. The following prompts will display:
a. Select
B44425AC 3-19
3.6 Powering Up Instrument
b. Select
c. Select .
The main screen is displayed (DxH 500 shown).
3-20 B44425AC
3.7 Waste Setup 3
3.7 Waste Setup
1 From the Main Menu, select the Supplies icon
2 From the Supplies screen, select the Waste Setup icon .
3 Select Waste Container or External Waste (sink) from the available menu
Select
WARNING
Risk of biohazardous contamination. If you have selected Waste Container for
your instrument's waste disposal, you must enter the correct size of the container
you are using. The default waste container size is 2,000 mL (0.53 gal). Failure to
select the correct waste container size can result in a biohazardous spill or having
to replace the container before it is full.
4 From the Supplies screen, select the Waste Setup icon .The screen prompt displays--
B44425AC 3-21
3.7 Waste Setup
5 Select
3-22 B44425AC
3.8 Reagent Setup 3
3.8 Reagent Setup
1 From the Main Menu, select the Supplies icon
2 From the Supplies screen, select the Setup icon
3 If you have a bar-code scanner, select from the Supplies Setup screen.
4 Using the handheld scanner, scan the 2D bar-code labels on each of the Diluent boxes.
5 Confirm information on label matches information on the screen.
6 When prompted the Question "Save Changes?" select
7 From the Warning window, select Change another reagent.
Select
B44425AC 3-23
3.8 Reagent Setup
8 Repeat for Lyse and Cleaner.
9 When all reagents are scanned, select Prime Reagent(s) then select
10 After Prime Cycles ends, open right side fluidics compartment door of the instrument using the
screwdriver supplied in accessory kit.
11 Check for any signs of liquid on bottom panel of the instrument. If any liquid is observed, power
instrument OFF and call your Beckman Coulter Representative.
3-24 B44425AC
3.9 Printer Setup 3
3.9 Printer Setup
1 Ensure printer is properly connected. Power printer ON.
2 Access the printer installation screen by pressing Setup > Admin Printer.
The connected printer is displayed on the screen as "Not Installed".
3 To install the printer, select the printer name. For example, "Lexmark MS310 Series."
The Printer Setup screen displays--
B44425AC 3-25
3.9 Printer Setup
4 Select
3-26 B44425AC
3.9 Printer Setup 3
5 Select the printer driver Lexmark_MS410_Series.ppd for the MS310 monochrome printer
supplied with the instrument.
6 Click on the Add icon to install the printer.

After a few seconds, the printer is displayed as "Installed."
B44425AC 3-27
3.9 Printer Setup
7 To set the printer as the default printer, select the printer name then select Default.
3-28 B44425AC
3.10 Select Printer 3
3.10 Select Printer
1 Select the Setup icon.
2 In the System Setup section, select the printer icon.
3 From the printer drop down, select the Lexmark-MS 310 series printer.
4 Select when finished.
B44425AC 3-29
3.10 Select Printer
3-30 B44425AC
3.11 Set Up Date and Time 3
3.11 Set Up Date and Time
1 Select the Setup icon.
2 In the System Setup section, select Date / Time .
3 Enter correct Date/Time and preferred format.
4 Select when finished.
B44425AC 3-31
3.11 Set Up Date and Time
3-32 B44425AC
3.12 Set Up ADMIN Account for Customer 3
3.12 Set Up ADMIN Account for Customer
An Admin account must be created for the customer because only Service has access to the Temp
Admin Password.
1 Select > .
2 On the Security Access Setup screen, select to add a user OR to edit existing user
information, highlight the user, and select .
3 Use the on-screen keyboard to enter the user information on the screen.
4 Select when prompted.
B44425AC 3-33
3.12 Set Up ADMIN Account for Customer
3-34 B44425AC
3.13 Daily Checks 3
3.13 Daily Checks
1 From the Main Menu, select .
2 Initiate a daily checks cycle by selecting the Daily Checks icon on the Daily Checks
screen.
3 Upon completion of the Daily Checks, ensure results are within the following specifications.
Parameter Result Limit Unit Status

WBC 0.00 ≤ 0.20 103/μL Pass
RBC 0.00 ≤ 0.03 106/μL Pass
HGB 0.00 ≤ 0.10 g/dL Pass
PLT (SW v1.x) 0.0 ≤ 10.0 103/μL Pass
PLT (SW v2.0 and future releases) 0.0 ≤ 7.0 103/μL Pass
B44425AC 3-35
3.13 Daily Checks
3-36 B44425AC
3.14 Check Instrument Settings and Adjustments 3
3.14 Check Instrument Settings and Adjustments
This section includes the following--
• System Configuration
• Check WBC Bath Filter (DxH 520 Only)
• Altitude and HGB Adjustments
• Optic LED Adjustment and WBC/ALL Gain Adjustment
• RBC Gain Check
• Check Tube Pierce Position (DxH 520 Only)
• Repeatability
• Calibration
• Controls
System Configuration
1 Log in as SERVICE.
2 Select Diagnostics icon.
3 Select SERVICE > SYSTEM CONFIG.
4 Verify that the Serial Number of the instrument is correct. If it is not, input the correct Serial
Number and notify your product specialist of the occurrence.
5 Verify the correct altitude setting:

a. For altitude </= 1500 meters, "enable altitude" radio button is NOT SELECTED.
b. For altitude > 1500 meters, "enable altitude" radio button IS SELECTED.
Check the WBC Bath Filter (DxH 520 Only)
1 Select > Drain Baths to drain the baths, vacuum, and waste syringes. When the power
button turns green, go to the next step.
B44425AC 3-37
2 Power OFF the instrument by pressing and holding the power button for a few seconds.
3 Select when the system prompts you to perform a shutdown before powering down.
4 Disconnect the power cord from the back of the instrument.
5 Open the right side of the instrument with a flathead screwdriver. When you release the screw,
take the panel off the hinges and move it out of the way.
6 Locate the WBC bath and place a wipe below the bath to catch any drips. The bottom of the WBC
bath contains the WBC bath filter.
7 Hold the tab on the WBC bath bottom and push it to the left so that it moves clockwise. This
aligns the slots on the bath bottom with the fastening screws on the underside of the bath.
3-38 B44425AC
8 Pull the bath bottom down to remove it from the WBC bath.
IMPORTANT Do not use an abrasive cloth or tissue. Do not scrub the filter. These could cause the filter to
scratch or tear.
9 Rinse the WBC bath filter with deionized water to remove all debris and visible particles.
10 Ensure the filter has no tears or damage of any kind. If any damage is seen, the filter must be replaced.
11 To reinstall the bath bottom, hold the tab on the bath bottom with one hand and align the slots
with the two fastening screws.
B44425AC 3-39
12 With a second hand, push up the bath bottom and verify that the fastening screws clear the bath
bottom.
13 Push the tab to the right to move it counterclockwise until the bath bottom is properly fastened.
14 Replace the door and secure it.
15 Reconnect the power cord to the back of the instrument. The flat side should face the waste
tubing.
16 Power ON the instrument by holding the power button for a few seconds. The instrument
software will start up.
17 Perform a Diluter Reset.
Perform System Verification Procedures specified in D.1, DxH 500 Series Post-Procedure -
Verification Matrix
3-40 B44425AC
HGB Adjustment
2 Select Diagnostics icon .
3 Select SERVICE > ADJUST OTHERS.
4 Press ADJ. HGB. Instrument will initiate self-adjust.
NOTE If the prompt "Diluter Reset Not Done" appears, go to the Diagnostics screen and perform Diluter
reset, then complete steps 2) and 3) again to self-adjust.
Optic LED Adjustment and WBC/ALL Adjustment

Refer to WBC/ALL Gain Adjustment under 4.7, Aperture Gain Checks and Adjustments.
IMPORTANT [Software v2.0 and future releases]

Once the Optic LED and/or WBC/ALL Gains have been adjusted, notify the customer of the date, time, and
ambient temperature when the adjustment took place. Document this information in your Service Call
Closeout.
This information will be used by the customer as a point of reference pertinent to the instrument
operational temperature. For details, refer to the instrument IFU, Chapter 1, System Overview -
Operational Temperature.
RBC Gain Check

Refer to RBC Check under 4.7, Aperture Gain Checks and Adjustments.
Check Tube Pierce Position (DxH 520 Only)

1. Obtain a specimen tube and run it as a sample in Closed-Vial mode.
2. When the cycle is completed, remove the tube and inspect the cap.
3. Ensure the cap of the tube was pierced in the center of the cap. If not centered, perform a cap
pierce adjustment (see 4.34, Cap Pierce Adjustments (DxH 520)).
B44425AC 3-41
Repeatability
To run Repeatability on the DxH 500, refer to the DxH 500 IFU, Chapter 11, Quality Assurance (PN
B95837). For the DxH 520, refer to the DxH 520 IFU, Chapter 11, Quality Assurance (PN B85528).
Calibration
The Calibration procedure is performed by the customer. For the DxH 500, refer to the DxH 500 IFU,
Chapter 11, Quality Assurance. For the DxH 520, refer to the DxH 520 IFU, Chapter 11, Quality Assurance.
Adjust parameters if required.
NOTE If calibration fails during installation, troubleshoot accordingly. A recommended troubleshooting step
is to perform a DI Water System Flush on the instrument (refer to 4.35, DI Water System Flush).
Controls
To run Controls on the DxH 500, refer to the DxH 500 IFU, Chapter 4, Quality Control. To run controls
on the DxH 520, refer to the DxH 520 IFU, Chapter 4, Quality Control.
If any level of control is out, troubleshoot parameter.
3-42 B44425AC
CHAPTER 4
Service and Repair Procedures
4.1 Guidelines for Servicing Instrument
General
Field Service Engineers can perform the procedures in this chapter using their standard tool kit.
Any special tools, supplies or equipment required are identified at the beginning of the applicable
procedure under the Tools/Supplies Needed heading.
NOTE A torque driver is not to be used to loosen components. Torque drivers should only be used to tighten
components to a specified torque.
When a service/repair procedure requires some type of instrument performance verification upon
completion, a verification heading is provided with the necessary steps that must be performed.
IMPORTANT [Software v2.0 and future releases] It is recommended that a BACKUP /RESTORE procedure
(see Backup or Restore [Software v2.0 and future releases]) is done upon initial installation and after
every calibration.
IMPORTANT You must drain baths prior to performing any disassembly procedure.
1. Select Diagnostics
2. Drain Baths
3. Power off the instrument.
The DxH 500 and DxH 520 will not cycle with the fluidics door open. During certain cycles, the
instrument will prompt you that the door is open. Press OK and the instrument will continue
cycling.
When servicing the instrument, log in as—
• Operator ID = SERVICE
• Password = BCIRAVEN (Software v1.x)
Temp Admin Password (Software v2.0 and future releases)
Guidelines
• Review and heed the general safety warnings and cautions listed under Safety Precautions.
B44425AC 4-1
• Always wear gloves, a lab coat, and eye protection when servicing the DxH 500 and DxH 520
analyzers to avoid biohazardous contamination.
• Always turn the instrument Off and unplug the power cord from back of instrument when
servicing.
• Be alert and use extreme care when working around exposed components to avoid personal
injury.
• Before removing or disassembling a component, note how the tube and/or wiring is dressed.
For certain components, how you dress the tube and/or wiring when you are finished is critical.
• At the end of the service call, always reinstall all instrument covers and remove any bypass to
prevent operator injury.
• At the end of a service call, always verify total instrument performance by doing a System
Verification. Refer to Check Instrument Settings and Adjustments.
• Current service procedures detail that parts for return must be decontaminated and placed in
their original shipping container or packaging, and that the container or packaging must
indicate the part was decontaminated. If BCI requests the shipment of any diagnostic specimen
or non decontaminated part for investigation, those particular types of returns must be
shipped in approved packaging.
Diagnostics
Software functions (Diagnostics) help you troubleshoot and calibrate the DxH 500 and DxH 520
instruments. To use the Diagnostics, follow the directions under Diagnostics in CHAPTER 7,
Troubleshooting.
Door Interlock
The DxH 500 and DxH 520 have one safety interlock switch for the fluidics door. It prevents the user
from cycling the instrument with the door open. Logging in as SERVICE bypasses the switch.
NOTE Ensure you are logged off of SERVICE before leaving the instrument with the customer.
Power Up/Power Down

To power down:
1. Hold down the ON/OFF button on the front panel until the Power Off message appears. A
warning message appears, allowing you the option to perform a Shutdown prior to a Power
Down. Make your selection and select checkmark icon .

2. To completely remove the power from the instrument, unplug the power cord from the back of
the instrument.
To power up:
1. Connect the power cord to the back of the instrument.
4-2 B44425AC
4.1 Guidelines for Servicing Instrument 4
2. Press the ON/OFF button on the front panel to turn on the power. (There is a delay from the time
the ON/OFF button is pressed till the display comes on.)
Backup or Restore [Software v2.0 and future releases]

The BACKUP/ RESTORE function backs up settings, gain values, CAL factors (WB & PD), motor
adjustments. and references. The function is performed at initial installation after all calibrations
are done.
CAUTION
Risk of damage to software. Always scan removable media (USB flash drive)
before connecting it to the instrument.
CAUTION
Risk of loss of results. Backup your files periodically. A restore overwrites the
information in the system. Before restoring data to the system, ensure that you
have a backup of the current system. Manual backups always overwrite the
contents of the backup hard drive.
1. Insert the USB flash drive into the USB port on the instrument. Backups will always overwrite
the current content of the USB flash drive.
2. Select > .
3. Back up or restore the information:
• To perform a backup, select the items and select .
• To perform a restore, select the items and select
4. Select when prompted, to perform the backup or restore.

5. When the backup or restore is completed, remove the USB flash drive from the USB port.
B44425AC 4-3
4-4 B44425AC
Service and Repair
4.2 Doors and Covers Removal 4
4.2 Doors and Covers Removal
The following procedures are covered in this section—
• How to Open and Remove the Left-Side Door [Reagent Compartment Door]
• How to Open and Remove the Right-Side Door [Fluidic Door]
• How to Remove the Top Cover
• How to Remove the Reagent Plate
• How to Remove the Secondary Reagent Plate
Tools/Supplies Needed
• Flat blade screwdriver

• T20 Torx head screwdriver
Turn the instrument off by holding down the ON/OFF button until the Power Off message appears,
and unplug the power cord from the back of the instrument.
How to Open and Remove the Left-Side Door [Reagent Compartment Door]
1 To open the left-side door (commonly called the Reagent Compartment Door), push the release
button to the back and swing open the door.
2 To remove door completely, lift door up and off the hinges.
B44425AC 4-5
Service and Repair
To reinstall the door, lift door onto hinges.
To close door, push door in until door latch locks.
How to Open and Remove the Right-Side Door [Fluidic Door]
1 To open the right-side door (commonly called the Fluidic Door), insert a flat blade screwdriver
into the two (2) captive screws and turn counterclockwise ¼ turn.
2 To remove door completely, lift door up and off the hinges.
To reinstall the door, lift door onto hinges.
4-6 B44425AC
Service and Repair
To close and lock door, turn the two (2) captive screws clockwise ¼ turn.
How to Remove the Top Cover

Open the right-side door (see How to Open and Remove the Right-Side Door [Fluidic Door]).
Open the left-side door (see How to Open and Remove the Left-Side Door [Reagent Compartment Door]
1 Loosen (no need to remove) the four (4) Torx screws securing the top cover to either side of the
instrument (two screws in rear and one on each side of instrument).
2 Lift top cover and remove it from the instrument.
To reinstall the top cover, tighten the four (4) Torx screws that secure top cover to instrument.
Reinstall the right and left-side doors.
B44425AC 4-7
Service and Repair
How to Remove the Reagent Plate

Turn the instrument Off and unplug the power cord from the back of instrument.
Remove right-side door (see How to Open and Remove the Right-Side Door [Fluidic Door]).
Remove left-side door (see How to Open and Remove the Left-Side Door [Reagent Compartment Door]).
Remove the top cover (see How to Remove the Top Cover
1 Remove the three (3) Torx screws at the back of the instrument. Remove the two Torx screws
inside the reagent compartment.
2 Remove panel by slightly lifting the plate up and sliding the section of the plate closest to the
front of the instrument out.
4-8 B44425AC
Service and Repair
3 Remove the grommet with the Cleaner and Lyse tubes by sliding it out of the cutout on the
panel. Remove the reagent plate from the instrument.
Reinstall reagent plate.

Reinstall top cover.
Reinstall left- and right-side doors.
How to Remove the Secondary Reagent Plate

Remove the top cover (see How to Remove the Top Cover)
Remove the reagent plate (see How to Remove the Reagent Plate)
B44425AC 4-9
Service and Repair
1 Remove the four (4) Torx screws at the front panel of the instrument.
2 Remove the remaining two screws securing the secondary reagent plate located underneath
the instrument. Remove the secondary reagent plate from the instrument.
3 Reverse steps for installation.
4-10 B44425AC
Service and Repair
4.3 Front Cover Assembly and Components Removal 4
4.3 Front Cover Assembly and Components Removal
• Removal of Front Cover

• Removal of LCD
• Removal of Front USB Board
• Removal of ON/OFF Board
• Removal of Start Analysis Plate/ Switch (DxH 500 Only)

• Flat head screwdriver
Turn the instrument off by holding down the ON/OFF button for 6 seconds, and unplug the power
cord from the back of the instrument.
Removal of Front Cover

Remove top cover (see How to Remove the Top Cover).
NOTE Before you begin, verify that the probe is NOT DOWN.
1 Loosen the two (2) Torx screws on top.
2 Tip the front cover forward.
B44425AC 4-11
Service and Repair
4-12 B44425AC
Service and Repair
3 To remove the front cover completely, all connections to the Mainboard must be removed.
1- Power Supply cable 14- LCD Power Supply + Backlight

2- Shear Valve/Tube Holder cable 15- Touch Screen flat cable
(DxH 520 Only) 17- Counting cable (and DxH 520 In-line
3- Electrovalves cable Pressure Sensor cable)
4- Heater cable (and DxH 520 Pump cable) 18- Optical LED
5- CPU-Rear Panel cable 19- Not used
6- Motors cable 20- WBC coaxial cable
9- ON-OFF cable 21- RBC coaxial cable
10- Optical coaxial cable 22- Instrument temp thermistor
11- USB cable 25- Start Switch cable
12- Not used 26- Not used
13- LCD Screen cable 27- Not used
B44425AC 4-13
Service and Repair
4 Remove vacuum tube from transducer. Note the location of tube; it must go on the outside
fitting.
5 Unscrew the USB board ground cable from the instrument front chassis.
6 Lift the front cover up to release it from the bottom securing tabs.
7 Remove front cover.
To reinstall front cover, lift cover onto bottom securing tabs.
Reinstall all Mainboard connections.
Tighten the two (2) Torx screws securing front cover to instrument.
Removal of LCD
Remove front cover (see Removal of Front Cover).
Remove all connectors from the Mainboard (see Removal of Mainboard).
1 Remove the seven (7) Torx screws that secure the LCD/CPU subassembly on front cover.
4-14 B44425AC
Service and Repair
2 Remove the LCD/CPU subassembly from the front cover.
3 Remove the four (4) Torx screws that secure the LCD support holder.
4 If not already done so, remove all connections from the Mainboard.
5 Remove the LCD.
Removal of Front USB Board

Turn instrument Off and unplug power cord from the back of the instrument.
Open the Front Cover Assembly.
1 Squeeze the two spring tabs together to release the USB board.
B44425AC 4-15
Service and Repair
2 Pull on the USB board and remove it.
3 Disconnect cable from USB board.
4 Unscrew the USB board ground cable from the main chassis.
5 Remove the board.
Removal of ON/OFF Board

Remove LCD/CPU assembly (see Removal of LCD and Removal of Mainboard).
1 Squeeze the two spring tabs together to release the ON/OFF board.
4-16 B44425AC
Service and Repair
2 Pull on the board and remove it.
3 Disconnect cable from ON/OFF board.
Removal of Start Analysis Plate/ Switch (DxH 500 Only)

Remove the Front Cover Assembly.
B44425AC 4-17
Service and Repair
1 Push on top of the start analysis plate to remove it from the instrument.
2 Disconnect the start switch from the CPU board (For details, see Removal of Mainboard,
Connection #25- Start Switch cable).
4-18 B44425AC
Service and Repair
3 With a T10 Torx head screwdriver, push on the rear of the switch to remove it from its location.
NOTE Record orientation of the switch prior to removal. It must be reinstalled in the same orientation.
When reinstalling, push switch firmly all the way into the holder. Looking at the switch from the
front of the instrument, ensure switch is in the same orientation as shown below.
When reinstalling plate, insert bottom of plate first before pushing top of plate in.
B44425AC 4-19
Service and Repair
4-20 B44425AC
Service and Repair
4.4 Power Supply Replacement 4
4.4 Power Supply Replacement
The power supply is an external stand-alone plug-in device and a simple replacement is all that is
required.
• None
1 Turn the instrument Off and unplug the power cord from the back of the instrument.
2 Remove power supply connector from back of instrument.
3 Connect new power supply connector to back of instrument.
4 Connect power supply power cord to wall outlet.
5 Turn the instrument On.
6 Verify Daily Checks passed.
7 Perform System Verification Procedures specified in APPENDIX D, DxH 500 Series Post-
Procedure - Verification Matrix.
B44425AC 4-21
Service and Repair
4.4 Power Supply Replacement
4-22 B44425AC
Service and Repair
4.5 Mainboard Replacement 4
4.5 Mainboard Replacement
• Removal of Mainboard
• Installation of Mainboard
• Replacement of Interface Board
Perform a backup of all data and system configuration settings. Refer to 4.1, Guidelines for Servicing
Instrument for details.
Turn the instrument Off and unplug the power cord from the back of the instrument.

• Controls or fresh blood specimens with known values (for Verification)
• Latex Particles and G-CAL
B44425AC 4-23
Service and Repair
Removal of Mainboard
1 Disconnect all connections from Mainboard.
1- Power Supply cable 14- LCD Power Supply + Backlight

2- Shear Valve/Tube Holder cable (DxH 520 15- Touch Screen flat cable
Only) 17- Counting cable (and DxH 520 In-line
3- Electrovalves cable pressure sensor cable)
4- Heater cable (and DxH 520 Rinse Pump 18- Optical LED
cable) 19- USB cable (front)
5- CPU-Rear Panel cable 20- WBC coaxial cable
6- Motors cable 21- RBC coaxial cable
9- ON-OFF cable 22- Not used
10- Optical coaxial cable 25- Start Switch cable
13- LCD Screen cable
4-24 B44425AC
Service and Repair
2 For the Touch Screen flat cable (15), use a small flat screwdriver to pry back the locking collar to
easily pull out the cable.
3 Disconnect the three (3) LCD cables from the left side edge of the Mainboard.
B44425AC 4-25
Service and Repair
4 Remove vacuum tube from transducer. Note the location of tube; it must go on the outside
fitting.
5 Remove the five (5) T10 Torx screws securing the Mainboard on the support holder.
6 Remove the Mainboard from the support holder.
Installation of Mainboard
1 Install new Mainboard with five (5) securing Torx screws.
2 Install all connections to new Mainboard.
NOTE Remember to connect the vacuum tube to the transducer.
NOTE Carefully slip the small display ribbon cable into connector and push the locking collar back in
while holding the ribbon cable in.
Reinstall top cover.
Reinstall right- and left-side doors.

Connect power cable to back of instrument.
4-26 B44425AC
Service and Repair
Power On instrument.
Login as SERVICE.
NOTE (Software v2.0 and future releases): Replacement mainboards have an internally stored generic
instrument serial number of "XXMMSSSS". Use this serial number when generating your Temp Admin
password.
Verify Daily Check passed.
Go to version screen.
Verify that software revision is latest.

Restore all previously backed up data and system configuration settings. Refer to 4.1, Guidelines for
Servicing Instrument for details.
Perform System Verification Procedures specified in APPENDIX D, DxH 500 Series Post-Procedure -
Verification Matrix.
Replacement of Interface Board

1 Remove reagent door.
2 Remove top cover.
3 Remove the reagent plate.
4 Remove three (3) screws securing board to rear cover.
5 Disconnect cables.
Reverse steps for installation.
B44425AC 4-27
Service and Repair
4-28 B44425AC
Service and Repair
4.6 Display Module Assembly Replacement 4
4.6 Display Module Assembly Replacement
Remove front cover (see Removal of Front Cover)
Remove Mainboard. (see Removal of Mainboard )

1 Remove the seven (7) T10 Torx screws securing the Display Module to the front panel.
2 Remove the four (4) T10 Torx screws securing the Display to the stainless steel plate.
Touchscreen Calibration
1 [Software 1.x] From the Login screen, press the aspiration paddle three (3) times.
[Software v2.0 and future releases] From the Login screen, press the instrument ON/OFF Button
three (3) times.
This will initiate the Touchscreen Calibration process.
B44425AC 4-29
Service and Repair
4.6 Display Module Assembly Replacement
2 Locate the "cross" on the top left corner of the screen. Using a stylus or pen, press the circle in
the center of the "cross".
3 Repeat step 2) for the remaining corners of the display.
NOTE If you exceed the time allowed to perform the alignment, you will need to restart the Touchscreen
calibration process by returning to Step 1).
4-30 B44425AC
Service and Repair
4.7 Aperture Gain Checks and Adjustments 4
4.7 Aperture Gain Checks and Adjustments
The Aperture Gain Checks and Adjustments includes the following:
• Optical LED Adjustment

• WBC/ALL Check
• WBC/ALL Gain Adjustment
• RBC Check
• RBC Gain Adjustment
Optical LED Adjustment

NOTE The Optical LED Adjustment must be performed with the dilutor door closed.
3 Select SERVICE > Adjust WBC.
4 Ensure the dilutor door is closed and perform Optical LED adjustment by selecting Adj. LED.
Confirm you want to perform LED adjustment by selecting in the Warning message.
Target value for ALL = 27500 +/- 3%.

Closeout.
WBC/ALL Check
The WBC/ALL Check procedure checks the WBC Mean Channel value and the Axial Light Loss (ALL)
Mean Channel value.
NOTE This check must be done with the right-side door and top cover both closed.
B44425AC 4-31
Service and Repair
• G-CAL (required for WBC and ALL gain adjustments only)
4 Perform Optical LED Adjustment (see Optical LED Adjustment).
5 Ensure the WBC Target value and ALL Target value on the screen are correct. If not, enter the
correct values found on the G-Cal assay sheet that came with the lot of G-Cal being used.
6 Ensure that the G-Cal has been thoroughly mixed and at room temperature.
7 Select Check WBC.
8 Immerse the sample probe into the G-Cal vial when prompted and select .
Note the following tolerance ranges for a WBC Check:
• WBC Mean Channel = WBC Target Value +/- 2
• ALL Mean Channel = ALL Target Value +/- 2
WARNING
To avoid being pierced by the aspiration probe, use caution when presenting
samples for analysis during this procedure.
9 If values are outside of the tolerance ranges, perform a second WBC Check.
If values continue to be outside of the performance ranges, refer to CHAPTER 7, Troubleshooting.
4-32 B44425AC
Service and Repair
WBC/ALL Gain Adjustment

This procedure includes the Optic LED Adjustment and the WBC/ALL Gain Adjustment. The WBC/
ALL Gain Adjustment performs both the WBC aperture gain adjustment and the Axial Light Loss
(ALL) optical gain adjustment.
• G-CAL (required for WBC and ALL gain adjustments only)
4 Perform Optical LED Adjustment (see Optical LED Adjustment).
5 Ensure the WBC Target value and ALL Target value on the screen are correct. If not, enter the
correct values found on the G-Cal assay sheet that came with the lot of G-Cal being used.
6 Ensure that the G-Cal has been thoroughly mixed and at room temperature.
7 Select Adj. WBC.
WARNING
8 Immerse the sample probe into the G-Cal vial when prompted and select .
9 Repeat step 8) two more times when prompted. The instrument requires three (3) aspirations
of G-Cal to perform the WBC Adjustment procedure. Note the following tolerance ranges for
WBC/ALL Gain Adjustments.:
• WBC Mean Channel = WBC Target Value ± 1
• ALL Mean Channel = ALL Target Value ± 1
B44425AC 4-33
Service and Repair
10 If values are correct, select to accept and confirm adjustment.

If values are outside of the performance ranges, refer to the WBC/Optic assembly
troubleshooting section in CHAPTER 7, Troubleshooting.
11 After adjustment has been made, run Repeatability, Verify Calibration, and Run Controls.

Closeout.
RBC Check
The RBC Check procedure checks the RBC Mean Channel.
• Latex Particles
3 Select SERVICE > Adjust OTHERS.
4 Ensure the Diameter listed in the Adjust RBC window is the same as the diameter of the latex
particles being used. If not, enter the correct diameter value found on the latex bottle being
used.
5 Ensure that latex has been thoroughly mixed and at room temperature.
6 Select Check RBC.
7 Immerse the probe into the latex vial.
4-34 B44425AC
Service and Repair
WARNING
8 Compare the RBC Mean Channel to the target value.

• RBC Mean Channel value must be target value ± 2
Target Values are dependent on the Mean Diameter of the RBC latex particles.
Diameter (µm) Target Value

Reference 5.01 42
Latex 5.021 43
Value
4.993 42
9 If value is outside of the performance range, perform a second RBC Check.
10 If values continue to be outside of the performance ranges, refer to CHAPTER 7, Troubleshooting
B44425AC 4-35
Service and Repair
RBC Gain Adjustment

The RBC Gain procedure performs the RBC aperture gain adjustment.
• Latex Particles
3 Select SERVICE > Adjust OTHERS.
4 Ensure the Diameter listed in the Adjust RBC window is the same as the diameter of the latex
particles being used. If not, enter the correct diameter value found on the latex bottle being
used.
5 Ensure that latex has been thoroughly mixed and at room temperature.
6 Select Adj. RBC.
7 Immerse the probe into the latex vial.
WARNING
8 Compare the RBC Mean Channel to the target value.

• RBC Mean Channel value must be target value ± 1
Target Values are dependent on the Mean Diameter of the RBC latex particles.
Diameter (µm) Target Value

Reference 5.01 42
Latex 5.021 43
Value
4.993 42
9 If values are outside of the performance ranges, refer to CHAPTER 7, Troubleshooting.
4-36 B44425AC
Service and Repair
10 After adjustment has been made, run Repeatability, Verify Calibration, and run Controls.
B44425AC 4-37
Service and Repair
4-38 B44425AC
Service and Repair
4.8 Heater Assembly Removal 4
4.8 Heater Assembly Removal
Drain the instrument from any liquids by removing the lyse, cleaner, and diluent pickup tubes and
performing two Prime All cycles.
Turn instrument Off and unplug power cord from the back of the instrument.
Remove top cover (see How to Remove the Top Cover)
Remove the reagent plate (see How to Remove the Reagent Plate).
• T10 screwdriver
• T20 screwdriver
1 Remove the three (3) heater Torx screws securing heater.
2 Disconnect the four (4) tubes from the heater.
B44425AC 4-39
Service and Repair
4.8 Heater Assembly Removal
3 Disconnect the heater connector.
4 Remove the heater from the instrument.
Use Hydraulic/Pneumatic schematic to confirm proper connections.
4-40 B44425AC
Service and Repair
4.9 Sampling Probe/Rinsing Head Replacement 4
4.9 Sampling Probe/Rinsing Head Replacement
This section also includes replacing the rinsing head o-ring.
• None
1 Move probe rocker back until it is on top of the baths.
2 Pull on the top of the probe slightly to remove it from the probe carriage.
3 Push down and pull on the rinsing head to remove it from the rocker.
B44425AC 4-41
Service and Repair
4 Pull up the probe to remove it from the rinsing head.
5 To replace the probe, disconnect it from the aspiration tube and remove it from the instrument.
Reverse steps for installation of new probe.

When installing new probe, trim approximately 1/16th inch from tube.
6 To replace the rinsing head, disconnect vacuum tube and diluent tube and remove from
instrument.
Reverse steps for installation of new rinsing head.
4-42 B44425AC
Service and Repair
Perform System Verification Procedures specified in APPENDIX D, DxH 500 Series Post-
Rinsing Head O-Ring Replacement

• None
1 Remove sample probe (see Sampling Probe/Rinsing Head Replacement).
2 To replace the O-ring in the rinsing head, unscrew the black probe guide and remove it from the
rinsing head.
3 Use the probe to remove the O-ring by inserting and removing the probe from the rinsing head.
4 Remove the O-ring from the probe and replace it.
B44425AC 4-43
Service and Repair
NOTE The DxH 520 Rinse Head includes a spacer positioned underneath the O-ring. When replacing the
O-ring on a DxH 520, ensure the spacer is present and properly positioned.
5 To replace the O-ring, insert the probe guide first, then the O-ring on the probe.
6 Install this assembly on the rinsing head and tighten the probe guide.
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Service and Repair
Reverse steps for installation of the probe and the rinsing head back onto the rocker assembly.
B44425AC 4-45
Service and Repair
4-46 B44425AC
Service and Repair
4.10 Sampling/Rocker Assembly Removal (DxH 500) 4
4.10 Sampling/Rocker Assembly Removal (DxH 500)
Drain the instrument from any liquids.
Remove the sample probe and rinsing head (see 4.9, Sampling Probe/Rinsing Head Replacement).

1 Loosen (Do Not Remove) the two (2) Torx screws securing the rocker guide.
2 Move the rocker guide to the down position.
3 Remove the probe home optical sensor Torx screw.
B44425AC 4-47
Service and Repair
4 Remove the probe sensor from the assembly.
5 Loosen the Torx screw that secures the rocker assembly to the rocker bearing.
NOTE Be extra careful not to drop the special square nut that is under the screw.
6 Pull on the rocker assembly to disengage it from the rocker bearing. The assembly removed
includes the sample probe holder and the sample probe belt.
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Service and Repair
7 Pull tubing out of the assembly location, then out of the cable tie and remove the assembly from
the instrument. A new cable tie may be needed when reassembling. Note how the tubing is
dressed.
8 To replace the sample probe carriage and sample probe belt (see 4.17, Probe Belt and Probe
Carriage Replacement (DxH 500)).
When reinstalling the rocker assembly, be very careful not to overtighten any of the screws since
this can lead to a cracked component or stripped threads.
After rocker assembly has been secured, move the rocker guide up until it engages with the bottom
of the rocker assembly, then move it down slightly so as not to cause friction between the two
components. Once properly engaged, there should be no play on the Rocker assembly and it should
move with even resistance along its track.
Tighten the screw and check again that there is no friction between the components.
B44425AC 4-49
Service and Repair
4-50 B44425AC
Service and Repair
Remove the sample probe and rinsing head (see 4.9, Sampling Probe/Rinsing Head Replacement).

1 Loosen (but do not remove) the two (2) Torx screws securing the rocker guide.
2 Move the rocker guide to the down position.
B44425AC 4-51
Service and Repair
3 Open (but do not remove) the front cover of the instrument and locate the cable for the probe
sensor board. Disconnect this cable and pull cable and connector out of the right instrument
panel.
4 Loosen the belt fixation set screw. Do not loosen this screw too much or the rectangular nut will
fall out from behind the Rocker Assembly.
5 Move the rocker out and loosen the belt fixation tensioning screw from underneath the probe
belt.
4-52 B44425AC
Service and Repair
6 Remove the screw located below the rocker motor gear.
7 Loosen (do not remove) the screw located above the rocker motor gear.
8 Access the rocker module fixation bracket and rotate counter-clockwise so that it clears the
gear.
B44425AC 4-53
Service and Repair
9 Retighten the screw, and pull on the rocker assembly to disengage it from the rocker bearing.
The assembly removed includes the sample probe holder and the sample probe belt.
When reinstalling the rocker assembly, be very careful not to overtighten any of the screws since
this can lead to a cracked component or stripped threads.
After rocker assembly has been secured, move the rocker guide up until it engages with the bottom
of the rocker assembly, then move it down slightly so as not to cause friction between the two
components. Once properly engaged, there should be no play on the Rocker assembly and it should
move with even resistance along its track.
Tighten the screw and check again that there is no friction between the components.
4-54 B44425AC
Service and Repair
4.12 Rocker Motor Replacement 4
4.12 Rocker Motor Replacement


Removal
1 Disconnect the rocker motor connector. Note orientation of connector in reference to top of
instrument. Be sure to install in same orientation.
2 Remove the two (2) Torx screws securing the rocker guide.
B44425AC 4-55
Service and Repair
3 Remove the Rocker Guide, exposing two (2) additional Torx screws that secure the Rocker
Motor.
4 Unscrew the two (2) Torx screws and remove the rocker motor.
Installation
1 To replace the rocker motor, first install the Torx screw of the motor.
2 Install the left screw of the motor and move the rocker to the left to engage the rocker gear rack
on the rocker motor gear pinion.
NOTE The left side screw opening is a vertical slot.
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Service and Repair
4.12 Rocker Motor Replacement 4
3 Push down on the screw to ensure pinion gear teeth are firmly meshed with the teeth on the
rack. Tighten screw.
NOTE Apply a small amount of grease on the rocker motor gear.
Reinstall the Rocker Guide. Move the rocker guide up until it engages with the bottom of the rocker
assembly, then move it down slightly so as not to cause friction between the two components. Once
properly engaged, there should be no play on the Rocker assembly and it should move with even
resistance along its track.
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Service and Repair
4-58 B44425AC
Service and repair
4.13 Rocker Position Sensor Replacement 4
4.13 Rocker Position Sensor Replacement
1 Remove the two (2) Torx screws securing the rocker optical sensor.
2 Disconnect the rocker sensor from its connector.
3 Gently pull on the rocker sensor to remove it from its location.
B44425AC 4-59
Service and repair
4.13 Rocker Position Sensor Replacement
4-60 B44425AC
Service and Repair
4.14 Probe Motor Replacement 4
4.14 Probe Motor Replacement

Remove the sampling/rocker assembly from its location (see 4.10, Sampling/Rocker Assembly
Removal (DxH 500)).
1 Disconnect the probe motor connector.
2 Remove the two (2) Torx screws securing the probe motor. Remove the motor.
B44425AC 4-61
Service and Repair
4.14 Probe Motor Replacement
4-62 B44425AC
Service and Repair
4.15 Probe Position Sensor Replacement (DxH 500) 4
4.15 Probe Position Sensor Replacement (DxH 500)

1 Remove the probe sensor Torx screw.
NOTE Sensor is held by one screw only.
2 Gently pull up the probe sensor to remove it from the assembly.
3 Disconnect the probe sensor connector and remove it from the instrument.
B44425AC 4-63
Service and Repair
4.15 Probe Position Sensor Replacement (DxH 500)
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Service and Repair
4.16 Probe Position Sensor Board Replacement (DxH 520) 4
4.16 Probe Position Sensor Board Replacement (DxH 520)

Remove the rocker assembly (see Sampling/Rocker Assembly Removal (DxH 520)).
1 Remove the two probe sensor board screws from behind the rocker assembly.
2 Gently pull up the probe sensor board to remove it from the assembly.
B44425AC 4-65
Service and Repair
4.16 Probe Position Sensor Board Replacement (DxH 520)
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Service and Repair
4.17 Probe Belt and Probe Carriage Replacement (DxH 500) 4
4.17 Probe Belt and Probe Carriage Replacement (DxH 500)

Remove the sampling/rocker assembly from its location (see 4.10, Sampling/Rocker Assembly
Removal (DxH 500)).

1 Remove the Torx screw securing the guide bar to the sampling assembly.
2 Remove the guide bar from the sampling assembly.
NOTE If needed, loosen the bottom tension gear to allow easier access to belt.
B44425AC 4-67
Service and Repair
3 Slowly work belt out then remove both the probe carriage and belt from the sampling
assembly.
4 Remove the Torx screw securing the belt to the probe carriage.
5 Slide the belt out to remove it from the carriage.
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Service and Repair
Replace belt or carriage and re-assemble in reverse order.
NOTE With the belt in the proper position over the motor gear and the tension gear, push down on the
tension gear until the proper belt tension is achieved then tighten the screw.
B44425AC 4-69
Service and Repair
4-70 B44425AC
Service and Repair

Remove the sampling/rocker assembly (see Sampling/Rocker Assembly Removal (DxH 520)).

1 Remove the Torx screw securing the guide bar to the sampling assembly.
2 Remove the guide bar from the sampling assembly.
B44425AC 4-71
Service and Repair
3 Remove the belt tensioner set screw and belt tensioning screw on the underside of the rocker
module. Remove both the probe carriage and belt from the sampling assembly.
4 Remove the Torx screw securing the belt to the probe carriage.
5 Slide the belt out to remove it from the carriage.
Replace belt or carriage and re-assemble in reverse order.
NOTE With the belt in the proper position over the motor gear and the tension gear, tighten the tensioning
screw underneath the rocker assembly until the proper belt tension is achieved then tighten the set screw
on the tension gear.
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Service and Repair
4.19 RBC Counting Chamber Replacement 4
4.19 RBC Counting Chamber Replacement
Drain RBC counting chamber (select DIAGNOSTICS > DRAIN BATHS).
Remove right-side door (see How to Open and Remove the Right-Side Door [Fluidic Door])).
• T10 Torx Tip

• T20 Torx Tip
• Adjustable torque screwdriver (set to 50cNm or 71 in-oz)
1 Loosen (do not remove) the Torx screw securing the splash shield. Remove shield.
2 Disconnect the red coaxial cable from the baths /counting manifold.
B44425AC 4-73
Service and Repair
3 Remove the four (4) Torx screws securing the RBC counting bath.
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4.19 RBC Counting Chamber Replacement 4
4 Pull bath back and out of instrument.
NOTE The location of the black ground wire on the RBC chamber is critical to the performance of the
instrument. This ground wire is an integral part of the aperture electrode circuit; it must always be
placed on the bottom right screw of the RBC chamber.
NOTE The O-ring located on the red fitting must stay in place, and only removed if it needs to be
replaced. The three other O-rings must stay in place on the RBC counting chamber, unless they need
to be replaced.
Reinstallation of the RBC counting chamber requires the use of an adjustable torque screwdriver.
Tighten screws in a cross pattern to 50cNm (71 in-oz).
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Service and Repair
4-76 B44425AC
Service and Repair
4.20 WBC Chamber /Optics Bench Replacement 4
4.20 WBC Chamber /Optics Bench Replacement
The WBC chamber/optics bench is to be removed/replaced as a single component. It consists of the

WBC chamber, WBC aperture, Hgb pre-amp, Hgb LED, collimator, ALL LED, and optics board. The
only removable and replaceable components on the DxH 500 and DxH 520 WBC optic benches are
the HGB LED and the WBC bath filter (DxH 520 only).
Internal alignment of the DxH 500 and DxH 520 optic benches can only be done by manufacturing
using automated alignment equipment.
Drain WBC counting chamber.
• T10 Torx Tip

• T20 Torx Tip
• Adjustable torque screwdriver (set to 50cNm or 71 in-oz)
1 Loosen (do not remove) the Torx screw securing the splash shield. Remove shield.
2 Disconnect the blue coaxial cable from the electronic board on the side of the WBC counting
chamber. Disconnect the white coaxial internal electrode cable from the counting manifold.
B44425AC 4-77
Service and Repair
3 Remove the cable from the counting board. Remove the optical LED power cable.
4 Remove the four (4) Torx screws securing the WBC counting chamber.
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Service and Repair
4.20 WBC Chamber /Optics Bench Replacement 4
5 Pull on the WBC Chamber/ Optics Bench then remove it from the counting manifold.
NOTE The location of the black ground wire on the WBC chamber is critical to the performance of the
instrument. This ground wire is an integral part of the aperture electrode circuit; it must always be
placed on the bottom left screw of the WBC chamber. Check to ensure that the O-ring on the external
electrode/ stainless steel fitting is present and in good condition.
NOTE The O-ring located on the red fitting must stay in place, and only removed if it needs to be
replaced. The four other O-rings must stay in place on the WBC counting chamber, unless they need
to be replaced.
Reinstallation of the WBC Chamber/ Optics Bench requires the use of a adjustable torque
screwdriver.
Tighten screws in a cross pattern to 50cNm (71 in-oz).
B44425AC 4-79
Service and Repair
4-80 B44425AC
Service and Repair
4.21 HGB LED Replacement 4
4.21 HGB LED Replacement
A new replaceable Hgb LED has been released for the DxH 500, beginning with instrument
SN AZ090463. The old LED configuration on the Optics Bench used a Hgb LED that was glued onto
the Optics Board. With the new configuration, the LED is secured by a bracket which is screwed onto
the Optics Board, allowing for easy LED replacement.
Old Style New Style
This change results in a new design to the Optics Bench as well as two new parts:
1. Hgb LED Upgrade Kit

2. Hgb LED
The Upgrade Kit consists of all components (including a Hgb LED) needed to upgrade the Optic
Bench from the old style configuration to the new style.
Prerequisites
Drain Baths (refer to IFU, Chapter 12. Cleaning Procedures).
Remove Optics Bench (refer to 4.20, WBC Chamber /Optics Bench Replacement).
Pliers (necessary for upgrading from old style configuration)
Torque Driver (set to 45 cNm or 64 in-oz)
T08 torx tip (PN B96003 or obtained locally)
B44425AC 4-81
Service and Repair
Removal
1 If working on new style Optics Bench configuration, proceed to Step 5.
2 If working on old style Optics Bench configuration, then the Hgb LED is glued onto the optics
board. Use pliers to twist the Hgb LED until the glue breaks and it can be removed.
3 Remove LED and discard. Ensure there is no glue or plastic remaining in the hole of the optics
board.
4 Locate and remove the screw on the bottom right corner of the optics board. This screw will be
replaced by a longer screw supplied in the Hgb LED upgrade kit. Proceed to Installation.
5 If working on new style Optics Bench configuration, loosen the screw securing the bracket and
slide bracket away so that the Hgb LED can be removed.
6 Remove LED and discard.
Installation
1 Obtain and insert the new LED into the hole in the optics board from which the previous LED
was removed. Push it in as far as possible.
2 With the torque driver set to 45 cNm (64 in-oz), install the new Hgb LED by screwing the bracket
into the bottom-right corner of the optics board, with the flange of the bracket applying force
evenly along the centerline of the Hgb LED for proper alignment.
NOTE Ensure the bracket is properly positioned and the Hgb LED is fixed in place.
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Service and Repair
4.21 HGB LED Replacement 4
Force
Correct applied at
placement centerline
of bracket of LED
(right side)
4425014A
Reinstall the WBC Optics Bench (refer to 4.20, WBC Chamber /Optics Bench Replacement).
Perform a DILUTER RESET cycle (refer to IFU Chapter 10, Troubleshooting).
Perform a HGB Adjustment (refer to 3.14, Check Instrument Settings and Adjustments.
B44425AC 4-83
Service and Repair
4-84 B44425AC
Service and Repair
4.22 Counting Manifold Assembly Replacement 4
4.22 Counting Manifold Assembly Replacement
Remove the RBC Chamber (see 4.19, RBC Counting Chamber Replacement).
Remove the WBC Chamber/Optics Bench (see 4.20, WBC Chamber /Optics Bench Replacement).

1 Disconnect the three (3) coaxial cables.
B44425AC 4-85
Service and Repair
4.22 Counting Manifold Assembly Replacement
2 Disconnect tubes #11; 4-B; 26-B; 17; 2-A and 1 as shown from the fittings of the counting
manifold. Refer to the hydraulic schematic for tube identification.
3 Remove the three (3) Torx screws securing the counting manifold assembly. Pull on the
assembly and remove it.
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Service and Repair
4.23 Solenoid Valve Replacement 4
4.23 Solenoid Valve Replacement
• T06 Torx Tip

• Adjustable torque screwdriver, set to 25 cNm (35 in-oz) or 40 cNm (57 in-oz)
1 Identify solenoid valve to be removed. Disconnect the connectors.
NOTE Solenoid valves are 2-way and 3-way type and have a specific orientation in the manifold. Note
the orientation of the valve prior to removal.
2 Remove the solenoid Torx screws.
3 Remove the valve body Torx screws.
B44425AC 4-87
Service and Repair
4.23 Solenoid Valve Replacement
4 Remove the valve body from the valve manifold.
NOTE When installing, valve body must be tightened to 40 cNm and valve solenoid must be tightened to 25
cNm.
When installing, note that valves come with O-rings already in place. Ensure that O-rings have not
fallen or shifted prior to installing valve.
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Service and Repair
4.24 Drain/Mixing Valve Manifold Replacement 4
4.24 Drain/Mixing Valve Manifold Replacement

1 Disconnect tubes #2-B; 1; 14-B and 14-C from the hydraulic connectors of the counting valves
manifold.
2 Disconnect the valves connectors.
3 Remove the two (2) Torx screws of the assembly.
B44425AC 4-89
Service and Repair
4.24 Drain/Mixing Valve Manifold Replacement
4 Pull on the assembly and remove it from the instrument.
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Service and Repair
4.25 Syringe Body/Pistons Replacement 4
4.25 Syringe Body/Pistons Replacement
• T20 Torx Tip

• Adjustable torque screwdriver (set to 60cNm and 35cNm)
• absorbent material
1 Remove the six (6) Torx screws securing the syringe body.
NOTE Place absorbent material around the motor shaft and motor to prevent any reagents from
entering this area.
NOTE There are sixteen (16) O-rings sealing the syringes to the syringe assembly. Be careful when
removing the syringes to avoid losing any of them.
2 Pull on the syringe body and remove it from the instrument.
NOTE Once the syringe body is removed, pay attention to the O-rings. During dismantling they can stay
onto the syringe body or on the manifold.
B44425AC 4-91
Service and Repair
4.25 Syringe Body/Pistons Replacement
3 Once the syringe body is removed, pull on the pistons to remove them if required.
Reverse steps for installation. Tighten the four (4) smaller upper screws to 60cNm.Tighten the two
(2) lower screws to 35cNm.
NOTE If any pistons require changing, do not apply lubricant at this time. Once the syringe assembly is
installed, follow the procedure for applying lubricant.
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Service and Repair
4.26 Syringe Assembly Replacement 4
4.26 Syringe Assembly Replacement
Remove the syringe body (see 4.25, Syringe Body/Pistons Replacement).
1 Disconnect all tubing from the rear of the syringe assembly. (Refer to the Hydraulic/Pneumatic
Schematic for re-installing tubes).
2 Disconnect the motor connector and the syringe home sensor connector.
B44425AC 4-93
Service and Repair
4.26 Syringe Assembly Replacement
3 Loosen the four (4) Torx screws securing the syringe assembly. Remove the ground cable Torx
screw from the syringe assembly.
NOTE It is not necessary to remove the screws from the assembly; the module is secured by four (4)
rubber shock mounts which are part of the syringe assembly.
4 Remove the syringe assembly from the instrument.
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Service and Repair
4.27 Syringe Optical Sensor Replacement 4
4.27 Syringe Optical Sensor Replacement

1 Disconnect the syringe sensor from its connector.
2 Remove the syringe sensor Torx screw.
3 Remove the sensor from the instrument.
B44425AC 4-95
Service and Repair
4.27 Syringe Optical Sensor Replacement
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Service and Repair
4.28 Syringe Valves Manifold Assembly Replacement 4
4.28 Syringe Valves Manifold Assembly Replacement

1 From the Syringe Valves Assembly, disconnect all tubing connected to other assemblies. Refer
to the Hydraulic/Pneumatic Schematic for re-installing tubes.
2 Disconnect all connectors from the valves.
3 Remove the four (4) Torx screws.
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Service and Repair
4.28 Syringe Valves Manifold Assembly Replacement
4 Remove the syringe valves assembly.
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Service and Repair
4.29 Tubing Replacement 4
4.29 Tubing Replacement
Liquid leaking from a tube that is not seated firmly on a fitting or from a tube that has popped off
of a fitting can migrate into solenoid valves, motors and electronic components, causing electrical
component failures and corrosion of the solenoid banks.
Use this procedure for replacing or re-installing tubing to ensure the tubing firmly grips the fitting.
NOTE The DxH 500 and DxH 520 instruments use stepper motor driven syringes to move reagents and make
dilutions. Because of this displacement technique, it is not possible to pinch off a tube to troubleshoot a
problem because the force of the syringe movement will cause a failure in the weakest point in the
system, usually the tube/fitting connection. If you need to test a specific line, remove the tube from the
fitting and place it in a container to catch the reagent.
• Absorbent material
• Sharp side cutters (for cutting the tube)
1 Before disconnecting a tube from a fitting, take precautions to prevent liquid from leaking onto
components, particularly in critical areas such as the optics bench area and the reagent syringe
lead screw and guide shaft area.
NOTE Precautions can include clamping tube and using absorbent material to catch leaks.
2 Carefully disconnect the tube from the fitting. The end of the tube will be stretched (flared)
from the "tube memory" of the fitting's shape as shown.
When re-installing, remove approx 1/8 inch from the flared tube and re-insert into fitting ensuring
the tube goes over the fitting bard.
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Service and Repair
4.29 Tubing Replacement
4-100 B44425AC
Service and Repair
4.30 Tube Holder Module Replacement (DxH 520) 4
4.30 Tube Holder Module Replacement (DxH 520)

Remove the front cover (see Removal of Front Cover)

1 Disconnect the tube holder door sensor cable from the tube holder sensors board located
behind the front panel.
2 Push the tube holder door sensor cable connector out from behind the grommet of the front
panel.
NOTE Do not pull on the cable or you may damage it.
3 Unscrew the four screws that secure the tube holder module to the front panel.
The outside two screws are secured from the back of the front panel using "nyloc" locknuts. To
remove these screws, first turn them clockwise to unfasten the nuts from the panel. Next, hold
the locknuts in place with a wrench while turning the screws counterclockwise (removes the
screws).
B44425AC 4-101
Service and Repair
4.30 Tube Holder Module Replacement (DxH 520)
4 Carefully pull the assembly out while turning it counter-clockwise. Disconnect the remaining
sensor cables. The colored, motor cable does not need to be disconnected.
NOTE The locknuts from Step 3 may remain unfastened from the front panel until after the front cover is
positioned. Once positioned properly, refasten the nuts by accessing the screws behind the front panel
and turning them counterclockwise.
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Service and Repair
4.31 Shear Valve Module Replacement (DxH 520) 4
4.31 Shear Valve Module Replacement (DxH 520)


1 Position the probe over the RBC bath for diluent drainage when tubing is removed from the
Shear Valve.
2 Disconnect the Shear Valve sensor connect and the Shear valve motor connector.
3 As a precaution, place absorbent material (paper towel, dry cloth, etc.) below the shear valve in
the event there is dripping from tubing removal (subsequent steps).
4 Disconnect tubing #9B (connects to sample syringe) from the Shear Valve (top left "IN" port).
5 Disconnect tubing #31A (connects to top port of RBC bath) from the Shear Valve (bottom left
"OUT" port) and allow diluent to drain into the RBC bath.
6 Ensure the probe is positioned above the RBC bath then disconnect tubing #9A (connects to
sample probe) from the Shear Valve (bottom right "IN" port). Diluent may drain from the probe
into the RBC bath.
B44425AC 4-103
Service and Repair
4.31 Shear Valve Module Replacement (DxH 520)
7 Disconnect tubing #31B (connects to VL12) from the Shear Valve (top right "OUT" port).
8 Unscrew the four (4) Torx screws that secure the Shear Valve bracket onto the instrument
chassis.
9 Remove the assembly.
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Service and Repair
4.32 Shear Valve Disassembly/Reassembly (DxH 520) 4
4.32 Shear Valve Disassembly/Reassembly (DxH 520)

Remove Shear Valve Module (see 4.31, Shear Valve Module Replacement (DxH 520)

Disassembly
1 Locate and remove the two screws that hold the shear valve motor onto the shear valve
mounting bracket and set the bracket aside.
2 Remove the four screws that secure the shear valve motor to the shear valve holder.
3 Pull the motor out of the module assembly and place it aside. The remaining parts consist of the
valve sensor, the valve motor holder, and the valve assembly.
B44425AC 4-105
Service and Repair
4 Remove the screw securing the shear valve sensor and set the sensor aside.
5 Unscrew the three screws securing the stator to the motor holder and remove it, exposing the
rotor. Notice the groove on the head of the rotor, which is used to segment 25uL of sample.
Depending on the orientation of the rotor, the shear valve switches from input to output.
Reassembly
1 Insert the rotor inside the stator. This creates the Shear Valve Head.
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Service and Repair
2 Align the Shear Valve Head with the Shear Valve Holder and screw them into place using three
(3) screws previously removed. Use the Shear Valve Sensor as an alignment tool.
NOTE This alignment is CRITICAL. Take care when aligning these two pieces.
3 Secure the shear valve screw using the screw that was previously removed. Ensure the rotor
disc aligns with the slot of the sensor.
B44425AC 4-107
Service and Repair
4 Rotate the motor until the dowel pin located on the motor lines up with the bottom of the rotor.
Continue to rotate the motor until the sensor and the Shear Valve Motor connector are located
on the same side of the assembly.
5 Use the four (4) screws previously removed to secure the shear valve motor to the shear valve
holder.
6 Use the two (2) screws previously removed to secure the shear valve assembly to the shear
valve mounting bracket. Ensure the orientation of the assembly with relation to the bracket is
correct.
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Service and Repair
B44425AC 4-109
Service and Repair
4-110 B44425AC
Service and Repair
4.33 Rinse Pump Replacement (DxH 520) 4
4.33 Rinse Pump Replacement (DxH 520)

Remove the secondary reagent plate (see How to Remove the Secondary Reagent Plate)
1 Locate and remove the two screws that hold the rinse pump onto the front panel of the
instrument.
2 Disconnect the tubing connecting the rinse pump. Remove the rinse pump.
B44425AC 4-111
Service and Repair
4.33 Rinse Pump Replacement (DxH 520)
4-112 B44425AC
Service and Repair
4.34 Cap Pierce Adjustments (DxH 520) 4
4.34 Cap Pierce Adjustments (DxH 520)
The DxH 520 Cap Pierce Adjustments include the following:
• Tube Holder Adjustment

• Piercing Position Adjustment
• Probe Bath Position Adjustment
Tube Holder Adjustment

Tools/Supplies Needed: None
1 Login as SERVICE.
2 Select SERVICE > CP ADJUST.
3 Select INIT TRAP DOOR to initialize the tube holder door motor.
4 Select ADJ. TRAP DOOR to begin the adjustment procedure.
5 Confirm the procedure by selecting the checkmark icon.
6 Select "Step -"on the screen several times until the tube holder door is slightly open.
7 Select "Step +" one step at a time until the tube holder door no longer has any play. Use your
finger to gently gauge the amount of play on the door during each motor step.
B44425AC 4-113
Service and Repair
NOTE (1) DO NOT push on the door too hard or it will cause the motor to mis-step. (2) If the tube holder
door makes a clicking sound or if you hear a slight grinding noise, the door has been over-adjusted
and you must repeat the adjustment procedure.
8 Once the proper motor steps have been made, select the checkmark icon.
Piercing Position Adjustment

1 Login as SERVICE.
3 Select INIT PROBE MECH. to initialize the rocker motor.
4 Select ADJ. PIERCING to begin the adjustment procedure.
6 Follow the on-screen adjustment instructions:
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Service and Repair
Step 1: Move the rocker assembly back such that you have access to the probe.
Step 2: Lower the probe about 2 cm.
Step 3: With the aid of the "Step + / Step -" buttons, position the probe as close as possible to
the tube holder assembly, without making contact.
Probe Bath Position Adjustment

1 Login as SERVICE.
3 Select INIT PROBE MECH. to initialize the rocker motor.
B44425AC 4-115
Service and Repair
4 Select ADJ. BATH to begin the adjustment procedure.
6 Follow the on-screen adjustment instructions:
Step 1: Move the rocker assembly back such that you have access to the probe and can position
it over the WBC bath.
Step 2: Lower the probe about 5 cm. and move the probe to the left until it makes contact with
the inside of the WBC bath.
Step 3: With the aid of the "Step -" button, move the probe to the right until it no longer makes
contact with the WBC bath.
Step 4: Select the "Step +" until the probe makes contact with the bath without bending the
probe.
4-116 B44425AC
Service and Repair
B44425AC 4-117
Service and Repair
4-118 B44425AC
Service and Repair
4.35 DI Water System Flush 4
4.35 DI Water System Flush
NOTE This system flush procedure uses deionized water instead of reagents which causes the instrument to
overestimate the reagent consumption. Inform the customer that when the supply warning or error
message is displayed, the reagent containers will contain excess volume.
Deionized water
Beaker (750 mL or greater)
Procedure
1 Log-in as SERVICE.
2 Open the left side reagent compartment door and remove the Lyse and Cleaner pick-up tubes
from their containers.
3 Remove the Diluent reagent pick-up tube from the Diluent container.
4 Insert all three pick-up tubes in the beaker with at least 500 mL of deionized water.
5 Navigate to the Supplies screen and perform a PRIME ALL cycle.
6 Navigate to Display/Run screen and run 10 blank samples. Ignore the results.
7 Navigate to Diagnostic screen and execute a BACKFLUSH APERTURE cycle three times.
8 Return to the Supplies screen and perform another PRIME ALL cycle.
9 Remove the reagent pick-up tubes from the beaker and place them on a clean surface.
10 Initiate an additional PRIME ALL cycle, which will eventually prompt a "No Diluent" error
message. Acknowledge the message.
B44425AC 4-119
Service and Repair
4.35 DI Water System Flush
11 Perform a Diluter Reset.
12 Repeat Steps 10 and 11.
13 Re-connect the reagent pick-up tubes into their respective reagent containers.
14 Perform a final PRIME ALL cycle.
15 Perform System Verification Procedures specified in APPENDIX D, DxH 500 Series Post-
4-120 B44425AC
CHAPTER 5
Maintenance Procedures
5.1 Maintenance Requirements
With the exception of the 3-Year PMI, all routine maintenance is performed by the customer.
Maintenance for the DxH 500 and DxH 520 instruments include both cleaning and replacement
procedures. Maintenance schedules and instructions are located in the DxH 500 v1 IFU manual, PN
B23922, the DxH 500 v2 IFU manual, PN B95837, or the DxH 520 IFU manual, PN B85528.
• For the cleaning schedule, see Chapter 12 in the IFU manual.
• For the replacement schedule, see Chapter 13 in the IFU manual.
When performing a service call, check the following components. If maintenance is needed, use the
cited procedures below.
Sample Probe and Probe Rinse Head

When servicing a DxH 500 or DxH 520, refer to Sampling Probe/Rinsing Head Replacement for removal
instructions if cleaning is needed.
Syringe Motor Lead Screw Lubrication

1. Access the Diagnostics screen by selecting the Diagnostics icon.
2. Select the PARK SYRINGE button. Power instrument OFF.
3. Place small amount of Lubricant SPG35 on gloved fingertip (about the size of a match head).
4. Spread on the upper part of the syringe motor lead screw.
Bleaching
Under Diagnostics, perform bleach cycle by pressing Bleach Cycle button and follow prompts. Refer
to Performing a Bleach Cycle in the IFU for bleach dilution strength.
Vacuum Tube Inspection/Replacement
1 Turn the instrument Off and unplug the power cord from the back of the instrument.
B44425AC 5-1
Maintenance Procedures
5.1 Maintenance Requirements
2 Remove front cover (see Removal of Front Cover under 4.3, Front Cover Assembly and
Components Removal).
3 Locate tube #24. If any liquid is present inside the tube, replace tube.
5-2 B44425AC
Maintenance
5.2 Preventive Maintenance Inspection 5
5.2 Preventive Maintenance Inspection
Preventive Maintenance Inspection (PMI) consists of the table below.
Table 5.2-1 Preventive Maintenance Inspection (PMI)
User Tech
Monthly Annually
(or (or
every every
1,000 18,000
Maintenance Daily Weekly cycles) cycles) As needed As needed 3 year
Cleaning the Instrument X X
Cleaning the Baths X
Bleach Cycle X
Lubricating Syringe Assembly Pistons X
Replacing the Rinsing Head O-Ring X
Draining the Baths X
Flushing the Apertures X
Cleaning the Bar-Code Scanner X
Setting Up/ Replacing Waste Disposal X
Setting Up/ Replacing Supplies X
Replacing the Bar-Code Scanner X
Replacing the Sample Probe X X
Rinsing Head X
Lubricating Syringe Motor Lead Screw X
Replacing vacuum tube (tube #24) X
connected to transducer
Replacing Syringe Block Assembly X
B44425AC 5-3
Maintenance
5.2 Preventive Maintenance Inspection
5-4 B44425AC
CHAPTER 6
Schematics
6.1 Summary
The following schematics were accurate at time of publication. Always verify correct revision of
these schematics when servicing the DxH 500/520.
• 6.2, DxH 500/520 - Power Distribution

• 6.3, DxH 500 Series - Modules/CPU Connections
• 6.4, Overall Fluidic Diagram (DxH 500)
• 6.5, Overall Fluidic Diagram (DxH 520)
• 6.6, Measurement - Fluidic Diagram
• 6.7, Diluent Line - Fluidic Diagram (DxH 500)
• 6.8, Diluent Line - Fluidic Diagram (DxH 520)
• 6.9, Lyse Line - Fluidic Diagram
• 6.10, Cleaner Line - Fluidic Diagram
• 6.11, Drain Baths - Fluidic Diagram
• 6.12, Sample Mixing - Fluidic Diagram
• 6.13, Waste Draining - Fluidic Diagram (DxH 500)
• 6.14, Waste Draining - Fluidic Diagram (DxH 520)
• 6.15, Blood Sampling - Fluidic Diagram (DxH 500)
• 6.16, Blood Sampling - Fluidic Diagram (DxH 520)
Test Points:
— (TP1) +24VDC External Power Supply Input
— (TP2) +24VDC Output of Protection IC
— (TP3) +24VDC Output of Main Power Switch
— (TP4) +12VDC Output DC/DC Converter for Backlight Power Supply
— (TP5) +3.3VDC Output of DC/DC Converter Power Supply
— (TP6) +5VDC Output of DC/DC Converter Power Supply
— (TP7) +3VDC Lithium Cell
B44425AC 6-1
Schematics
6.1 Summary
6-2 B44425AC
Schematics
6.2 DxH 500/520 - Power Distribution 6
6.2 DxH 500/520 - Power Distribution
B44425AC 6-3
Schematics
6.2 DxH 500/520 - Power Distribution
6-4 B44425AC
Schematics
6.3 DxH 500 Series - Modules/CPU Connections 6
6.3 DxH 500 Series - Modules/CPU Connections
B44425AC 6-5
Schematics
6.3 DxH 500 Series - Modules/CPU Connections
6-6 B44425AC
Schematics
6.4 Overall Fluidic Diagram (DxH 500) 6
6.4 Overall Fluidic Diagram (DxH 500)
B44425AC 6-7
Schematics
6-8 B44425AC
Schematics
6.5 Overall Fluidic Diagram (DxH 520) 6
B44425AC 6-9
Schematics
6-10 B44425AC
Schematics
6.6 Measurement - Fluidic Diagram 6
6.6 Measurement - Fluidic Diagram
B44425AC 6-11
Schematics
6.6 Measurement - Fluidic Diagram
6-12 B44425AC
Schematics
6.7 Diluent Line - Fluidic Diagram (DxH 500) 6
6.7 Diluent Line - Fluidic Diagram (DxH 500)
B44425AC 6-13
Schematics
6-14 B44425AC
Schematics
6.8 Diluent Line - Fluidic Diagram (DxH 520) 6
B44425AC 6-15
Schematics
6-16 B44425AC
Schematics
6.9 Lyse Line - Fluidic Diagram 6
6.9 Lyse Line - Fluidic Diagram
B44425AC 6-17
Schematics
6.9 Lyse Line - Fluidic Diagram
6-18 B44425AC
Schematics
6.10 Cleaner Line - Fluidic Diagram 6
6.10 Cleaner Line - Fluidic Diagram
B44425AC 6-19
Schematics
6.10 Cleaner Line - Fluidic Diagram
6-20 B44425AC
Schematics
6.11 Drain Baths - Fluidic Diagram 6
6.11 Drain Baths - Fluidic Diagram
B44425AC 6-21
Schematics
6.11 Drain Baths - Fluidic Diagram
6-22 B44425AC
Schematics
6.12 Sample Mixing - Fluidic Diagram 6
6.12 Sample Mixing - Fluidic Diagram
B44425AC 6-23
Schematics
6.12 Sample Mixing - Fluidic Diagram
6-24 B44425AC
Schematics
6.13 Waste Draining - Fluidic Diagram (DxH 500) 6
6.13 Waste Draining - Fluidic Diagram (DxH 500)
B44425AC 6-25
Schematics
6-26 B44425AC
Schematics
6.14 Waste Draining - Fluidic Diagram (DxH 520) 6
B44425AC 6-27
Schematics
6-28 B44425AC
Schematics
6.15 Blood Sampling - Fluidic Diagram (DxH 500) 6
6.15 Blood Sampling - Fluidic Diagram (DxH 500)
B44425AC 6-29
Schematics
6-30 B44425AC
Schematics
6.16 Blood Sampling - Fluidic Diagram (DxH 520) 6
B44425AC 6-31
Schematics
6-32 B44425AC
Schematics
6.17 Test Points 6
6.17 Test Points
There are seven (7) test points provided in this section located on the Mainboard. They are as
follows:
• TP1: +24VDC External Power Supply Input

• TP2: +24VDC Output of Protection IC
• TP3: +24VDC Output of Main Power Switch
• TP4: +12VDC Output DC/DC Converter for LCD Backlight Power Supply
• TP5: +3.3VDC Output of DC/DC Converter Power Supply
• TP6: +5VDC Output of DC/DC Converter Power Supply
• TP7: +3VDC Lithium Cell
(TP1) +24VDC External Power Supply Input
(TP2) +24VDC Output of Protection IC
B44425AC 6-33
Schematics
6.17 Test Points
(TP3) +24VDC Output of Main Power Switch
(TP4) +12VDC Output DC/DC Converter for Backlight Power Supply
6-34 B44425AC
Schematics
6.17 Test Points 6
(TP5) +3.3VDC Output of DC/DC Converter Power Supply
(TP6) +5VDC Output of DC/DC Converter Power Supply
B44425AC 6-35
Schematics
6.17 Test Points
(TP7) +3VDC Lithium Cell
6-36 B44425AC
CHAPTER 7
Troubleshooting
7.1 Diagnostics
Access to Diagnostics menu—
Select to display menu.
Button Action
HARDWARE RESET Resets syringe, probe and rocker motors to Home position. If motors are in Home
position it moves them out and back.
CLEAN BATHS Back flushes RBC and WBC apertures with cleaner; Drains baths and refills them
with diluent bath.
BACKFLUSH APERTURE Applies a back flush with cleaner to the WBC and RBC apertures. Drains and refills
baths with diluent.
B44425AC 7-1
Troubleshooting
7.1 Diagnostics
Button Action
BLEACH CYCLE Special cycle for cleaning the baths and aperture with a bleach solution.
Instrument will prompt operator through the steps.
DILUTER RESET Initiates HARDWARE RESET then performs cycle to ensure system is working
correctly.
PREPARE TO SHIP Runs a cycle to drain and clean the system before transport or extended storage.
Instrument will prompt operator through the cycle.
CHECK SENSORS This menu allows the user to check the functionality of the different instrument
sensors.
SERVICE Access to Service menu requires
• Operator ID: SERVICE
• Password: BCIRAVEN (Software v1.x)
Temp Admin Password (Software v2.0 and future releases)
DRAIN BATHS Drains the WBC and RBC baths.
RINSE BATHS Initiates a cycle that drains and refills the WBC and RBC baths with diluent.
LUBRICATION POS. Moves the syringes to the lubricate position for maintenance.
DILUENT DISPENSE Dispenses 300 ul of diluent to prepare dilution for pre-diluted mode.
PARK SYRINGE Moves the syringes UP to the PARK position. HARDWARE RESET brings the
syringes back to HOME position.
CHECK VALVES Access to Valves Test
This menu allows you to individually activate all the solenoid valves (three
seconds only) to check if they are being activated by the Driver board. Pressing
the buttons turns the valve On or Off. All valves can also be turned On
simultaneously (ALL VLs ON), or in sequence (EV CHASER). The description and
function of each valve are as follows:
DxH 500 Valves
Valve Description Function

V1 2-Way WBC Bath Drain - WBC Mix Opens path from WBC bath to vac/press syringes for draining
Bubbles and mixing bubbles.
V2 2-Way RBC Bath Drain - RBC Mix Opens path from RBC bath to vac/press syringes for draining
V3 2-Way Probe Wash Collar Vacuum Opens path from probe wash collar to vacuum/waste syringe.
Solenoid
V4 3-Way Diluent Syringe Prime Routes diluent from diluent container to diluent syringe.
V5 3-Way Diluent Routing Routes diluent to from V4 to Sample Syringe or VL6.
V6 3-Way Diluent Routing Routes diluent from VL5 to WBC Bath or VL12.
V7 2-Way Waste Valve Routes waste from Waste syringes to Waste container.
V8 2-Way Atmosphere Valve Connects Waste syringes to atmosphere for venting.
7-2 B44425AC
Troubleshooting
7.1 Diagnostics 7

V9 3-Way Lyse Priming Routes Lyse from Lyse container to Lyse syringe.
V10 2-Way Count Valve Routes vacuum from Waste Syringes to RBC and WBC baths.
V11 2-Way Cleaner Valve Routes Cleaner from Cleaner container to "Y" fitting.
V12 3-Way Diluent Routing Routes Diluent from VL6 to Rinsing head or rear of WBC bath.
DxH 520 Valves

V1 2-Way WBC Bath Drain - WBC Mix Opens path from WBC bath to vac/press syringes for draining
V2 2-Way RBC Bath Drain - RBC Mix Opens path from RBC bath to vac/press syringes for draining
V3 3-Way Diluent Routing Routes Diluent from VL12 to Rinsing head or rear of WBC bath.
V4 3-Way Diluent Syringe Prime Routes diluent from diluent container to diluent syringe.
V5 3-Way Diluent Routing Routes diluent to from V4 to Sample Syringe or VL6.
V6 3-Way Diluent Routing Routes diluent from VL5 to WBC Bath or VL12.
V7 2-Way Waste Valve Routes waste from Waste syringes to Waste container.
V8 2-Way Atmosphere Valve Connects Waste syringes to atmosphere for venting.
V9 3-Way Lyse Priming Routes Lyse from Lyse container to Lyse syringe.
V10 2-Way Count Valve Routes vacuum from Waste Syringes to RBC and WBC baths.
V11 2-Way Cleaner Valve Routes Cleaner from Cleaner container to "Y" fitting.
V12 3-Way RBC Dilution Transfer Routes Diluent from VL6 to RBC Bath or VL3.
Service
Access to Diagnostics-Technician menu—
Select SERVICE to display menu.
B44425AC 7-3
Troubleshooting
7.1 Diagnostics
Button Action
CBC THRESHOLDS FLAGS Access to CBC Thresholds Flags screen.
DIFF THRESHOLDS Access to Diff Thresholds screen.
DIFF FLAGS Access to Diff Flags screen.
ADJUST WBC Access to Adjust WBC screen. [Access for SERVICE ONLY]
ADJUST OTHERS Access to Adjust Others screen. [Access for SERVICE ONLY]
INTERNAL SETTINGS Access to automatic adjustments. [Access for R&D ONLY]
BURN IN Launches burn in cycle. [Available for MANUFACTURING ONLY]
SYSTEM CONFIG Access to System Config screen [Access for SERVICE ONLY]
CP ADJUST (DxH 520 Only) Access to Cap Pierce Adjustments screen (Not visible in DxH 500)
CBC Thresholds Flags

Screen provides access to thresholds and flag level settings.
7-4 B44425AC
Troubleshooting
7.1 Diagnostics 7
Diff Thresholds
Screen provides access to DIFF thresholds settings.
NOTE These settings are not to be changed unless specifically instructed to do so by Technical Support.
B44425AC 7-5
Troubleshooting
7.1 Diagnostics
Diff Flags
Screen provides access to DIFF flags level settings.
7-6 B44425AC
Troubleshooting
7.1 Diagnostics 7
Adjust WBC
Screen provides access to WBC Gain Adjustment and ALL Adjustment . [Access for SERVICE ONLY]
Button Action
ADJ LED Before LED light flux adjustment, the RINSE cycle is automatically run to clean the
aperture. Optical flow is adjusted nearest to the target 27500 with a tolerance of 3%.
If the adjustment fails, a prompt informs the operator. O-DF flag is set. (The DIF, DIF flags
and Scatterplot invalidated.
ADJ WBC Resistive WBC and optical gain adjustment are done using G-CAL for adjustment of gain
to optimally fit targets. The targets are editable by the operator.
Default Targets are:
• WBC: 47 ±1
• ALL: 83 ±1
CHECK WBC Resistive WBC and optical gain adjustment are done using G-CAL.
Default Targets are:
• WBC: 47 ±2
• ALL: 83 ±2
B44425AC 7-7
Troubleshooting
7.1 Diagnostics
Adjust Others
Screen provides access to RBC Gain and Hgb LED adjustments. [Access for SERVICE ONLY]
Button Action
ADJ PRESSURE Calibrates the pressure sensor regarding the entered altitude. Pressure measurement is
calibrated to give correct vacuum values for entered altitude.
Vacuum should be [0.042 * Altitude (in meters) - 415] in mBars.
ADJ HGB Launches Hgb LED flow adjustment process. Before LED light flux adjustment, the RINSE
cycle is automatically run to clean the bath. Hemoglobin flow is adjusted nearest to the
target. For software v1.1.1 and future releases, the target is 20,000 +/- 5%. For prior
releases, the target is 3,000 +/- 10%.
LED adjustment is rejected if the fluidic door is opened when logged in as SERVICE. If the
adjustment fails, a prompt informs the operator. The hemoglobin is invalidated until an
adjustment of the LED is successful.
ADJ RBC Adjusts the RBC gain based on the Latex diameter. Resistive RBC gain adjustment is
done using calibrated cells (Latex beads with diameter about 5 um) for adjustment of
gain to optimally fit targets.
Default Target is 42 ±1.
After Gain check or adjustment, RBC curve is displayed.
CHECK RBC RBC Resistive gain is checked using Latex particles.
Default Target is 42 ±2.
7-8 B44425AC
Troubleshooting
7.1 Diagnostics 7
Internal Settings
Not accessible to Service Engineers. [For R&D ONLY]
Burn In
This is a manufacturing cycle for testing reliability. [For FACTORY OR HIGHER]
CP Adjust (DxH 520)

Screen provides access to the Tube Holder and Probe Mechanism Adjustments.
Button Action
INIT TRAP DOOR Initiate the holder initialization
OPEN TRAP DOOR Open the sample trap door
CLOSE TRAP DOOR Close the sample trap door
ADJUST SAMPLE TRAP DOOR Initiate the adjustment cycle of the sample trap door
INIT PROBE MECH. Initiate the probe mechanism initialization
ADJUST PIERCING Initiate the adjustment cycle of probe mechanism for piercing
ADJUST BATHS Initiate the adjustment cycle of probe mechanism for postioning above
baths
B44425AC 7-9
Troubleshooting
7.1 Diagnostics
System Config
This menu allows you to change serial numbers, change instrument modes and enable altitude.
7-10 B44425AC
Troubleshooting
7.2 General Troubleshooting 7
7.2 General Troubleshooting
General troubleshooting is provided in the table below:
Situation Probable Cause Recommended Action

Power will not turn on. Power cord loose or not securely Make sure power cord is securely
connected to wall or instrument. connected to instrument, to power
supply, and wall.
Turn Power ON.
No voltage or incorrect voltage at Ensure voltage is on and outlet is
laboratory power outlet. 90-264 Vac.
Defective power switch. Replace component.
Instrument malfunction. Troubleshoot and replace
component if needed.
Grinding noise during initial power Component may have come loose Turn Power OFF, open diluter door,
ON. during shipment. Motors not look for any loose material or
reaching home. components.
Turn Power ON. If noise persists,
troubleshoot.
Screen is dark. Defective display or loose Replace component, inspect
Power button is lit. connections. connections from display to
Mainboard.
No aspiration takes place. Instrument not in Sample Change to Sample Analysis-
Cycle does not start. Analysis- Patient Result screen. Patient Result screen.
Defective Aspiration Plate, loose Inspect connection, replace
connector. component.
Defective aspiration switch Replace component.
located behind aspiration plate
(DxH 500 only).
Sample drips from probe area Fluid drips from inside the probe. This is a leak in the aspiration
after aspiration. pathway. Open right-side door and
check for loose tubing on Sample
syringe or V5. Check for bent
sample piston.
Fluid drips from outside the probe. The rinsing head is not working,
no vacuum. Open diluter side door
and check rinsing head O-ring,
loose/pinched tubing on rinsing
head, V3, or waste syringes.
Component failure. Replace component.
B44425AC 7-11
Troubleshooting
7.2 General Troubleshooting
Situation Probable Cause Recommended Action

WBC, RBC and/or Plt exceed Reagent lines not connected Verify reagent lines are tight and
background limits. Hgb correctly. in correct locations. Refer to
background may also be high in Installation procedure.
noted instances.
Instrument not primed correctly. Perform PRIME ALL function in
Supplies screen.
Contaminated diluent. Replace diluent. Perform PRIME
DILUENT in Supplies screen and
perform Daily Checks.
Contaminated baths. Perform CLEAN BATHS cycle in
Diagnostics screen. Perform Daily
Checks.
Micro bubbles in both baths. Check reagent pickup tube for
bubbles. Remove top cover and
check for bubbles on tube coming
in from back panel to V4 and on
tube from V4 to V5. Perform Daily
Checks.
Perform PROBE CLEANER in
Maintenance screen.
7-12 B44425AC
Troubleshooting
7.3 Error Message List 7
7.3 Error Message List
An Error Message listing is provided in the table below. For additional troubleshooting information
not available in this manual, consult the Beckman Coulter Advisor Knowledebase Management
System.
Error Message/ Event Type Consequence Detailed Recommendation

Prompt Description
Bath Drain Error ERROR Emergency stop Vacuum failure - Defective VL1 or VL2.
during bath draining - Tube on bath draining
in analysis cycle. path is pinched,
disconnected, or
damaged.
- Defective VL8.
- [DxH 520 only] WBC
Bath Filter is damaged or
requires maintenance.
Count Vacuum Error ERROR Emergency stop Vacuum failure - Defective VL10.
during counting - Tube on counting path is
phase in analysis pinched, disconnected,
cycle. or damaged.
- Defective VL8.
Cycle Busy ERROR Emergency stop Cycle cannot be 1. Wait until the current
initiated. cycle is completed.
2. Power off and Power
on the instrument.
Syringe Vacuum ERROR Emergency stop Vacuum failure - Tube #10 pinched,
Error during test syringe disconnected, or
cycle. damaged.
- Rinsing head clogged.
- Probe O-ring is
damaged.
- Defective VL3 or VL8.
Waste Drain Error ERROR Emergency stop Pressure failure - Waste tube is pinched.
during test syringe - Defective VL7.
cycle (on draining
waste from syringe). - Tube #5 is pinched.
No Diluent ERROR Emergency stop Vacuum failure at - Diluent supply is low.
begin of bath Check diluent.
draining: NO - Defective valve(s) along
DILUENT diluent pathway.
- Pinched, damaged, or
clogged tubing along
diluent pathway.
B44425AC 7-13
Troubleshooting

Prompt Description
Pre Probe Rinsing ERROR Emergency stop Vacuum failure - Loss of syringe motor
Vacuum Error. before probe rinsing. steps.
DxH 500 Only Not enough flow in - Rinse head cleaning
the rinsehead pathway clogged.
cleaning pathway
(drop in vacuum is - Defective VL3 or VL8.
<10% from when
aspiration occured).
Post Probe Rinse ERROR Emergency stop Vacuum failure after - Tube #10 is pinched.
Vacuum Error. probe rinsing. Not - Rinse head is clogged.
Specimen may be enough flow in the
diluted. Discard rinse head cleaning - Defective VL3 or VL8.
specimen. pathway (drop in
DxH 500 Only vacuum is <20%
compared to Pre-
Probe Rinse Vacuum
Check).
Syringe Vacuum ERROR Emergency stop Vacuum stability - Leak in waste pistons.
Error check failed during Check O-rings.
syringe vacuum. - Leak on tubing
connected to waste
syringes.
- Defective VL8 or fitting.
- Leak on tube #29.
Pre Aspiration ERROR Emergency stop Vacuum stability - Leak on tubing
Syringe Vacuum check failed before connected to waste
Error sample aspiration. syringes.
(limits = -700 to - - Leak on tubing
300 mBar). connected to counting
manifold.
- Defective VL3, VL8, or
VL10.
Post Aspiration ERROR Emergency stop Vacuum stability - Tube #10 pinched.
Syringe Vacuum check failed during - Rinsing head partially
Error sample aspiration. clogged.
(limits = -700 to -
300 mBar). - Defective probe O-ring.
- Probe guide too tight.
Loosen probe guide.
- Leak on tubing
connected to waste
syringes.
7-14 B44425AC
Troubleshooting

Prompt Description
Count Vacuum Error ERROR Emergency stop Vacuum stability - Leak on tubing
check failed during connected to waste
counting vacuum. syringes.
- Leak on tubing
connected to counting
manifold.
- Defective VL3, VL8, or
VL10.
No Bleach in Bath ERROR Emergency stop Detected air on No bleach or not
bleach cycle. sufficient bleach
introduced into each
bath.
Bleach Cycle ERROR Emergency stop Bleach Cycle was Perform a Diluter Reset
Interrupted interrupted. (e.g. and restart the bleach
operator initiated an cycle.
emergency stop,
operator initiated a
Power OFF, AC line
voltage (power
failure), etc.).
No Deionized Water ERROR Emergency stop Detected air in No DI water or
in Bath pathway during insufficient DI water
bleach cycle. introduced into each
bath.
X home position not ERROR Emergency stop HOME NOT FOUND. - Loss of x motor steps.
found MOTOR X - Damaged sensor.
- Damaged/disconnected
sensor cable.
X home error ERROR Emergency stop HOME FAILURE. Damaged/disconnected
MOTOR X sensor or cable.
X move error ERROR Emergency stop STEP LOSS. MOTOR Check for mechanical
X jam.
VL X Error ERROR Emergency stop COMMAND VALVE - Check connection.
FAILED. VALVE X - Burnt fuse.
- Defective valve
solenoid.
Maximum reagent ERROR A prompt informs Reagent - Bad connection on
temperature the operator: temperature >60°C heater assembly.
reached. Heating Reagent - Defective heater
stopped. temperature over assembly.
60. Heating is
stopped. - Defective mainboard.
Run sample
inaccessible.
B44425AC 7-15
Troubleshooting

Prompt Description
Reagent heating ERROR Heating is stopped; Reagent heating at - Bad connection on
stopped. a prompt informs 100% and no heater assembly.
the operator: temperature - Defective heater
Reagent heating increase during 2 assembly.
failed. min (0.5 increase)
- Defective mainboard.
Run sample
inaccessible.
Cannot analyze ERROR Run sample If the reagent Reagent temperature not
specimens because inaccessible temperature is < reached. Wait for
the reagent (except with [target - 2.5°C] message indicating
temperature is out SERVICE rights). temperature has been
of range. These conditions reached.
disable the probe
down function.
The error prompt
appears when
operator goes in
Display/Run screen
where you can run
sample.
Instrument ERROR Run sample is If the ambient - Check ambient
Temperature Out of allowed. For temperature is < temperature.
Range. software v1.1.1 and 18°C or > 34.5°C - Defective ambient
future releases, all temperature sensor.
results are flagged
with R flags.
The error prompt
appears when
operator goes in
the Display/Run
screen.
HGB LED ERROR Hemoglobin is HGB LED ADJUST - Check connections.
Adjustment failed. invalidated. failed. For software - Replace component.
v1.1.1 and future
releases, the target
is 20,000 +/- 5%. For
prior releases, the
target is 3,000 +/-
10%.
OPTIC LED ERROR Optical Adjust OPTICAL LED - Check connections and
Adjustment failed. failed. ADJUST failed (out of alignment of LED.
27500 ±3%) - Adjust Optical LED and
verify WBC/ALL gains
with G-Cal.
- Replace component.
7-16 B44425AC
Troubleshooting

Prompt Description
Auto Rinse traceability N\A Rinsing cycle run. Wait for cycle to
initiated. complete.
Auto Clean traceability N/A Auto cleaning cycle Wait for cycle to
initiated. run. complete.
DILUTER DOOR ERROR Emergency stop. Opening Diluter - Diluter door is open.
OPENED door. Close door.
- Adjust door switch.
- Door switch is
defective.
Vacuum Pump Error ERROR Emergency stop. Insufficient Vacuum Run Dilutor Reset
DxH 520 Only on Rinse Pump Check Rinse Pump
connections, fittings,
tubing.
Replace as needed.
Sample Trap Door ERROR Emergency stop. Tube Holder Door Inspect for obstructions.
Error Occurred does not reach close Verify alignments.
While Closing position when
closing Replace sensors if
necessary.
Sample Trap Door ERROR Emergency stop. Tube Holder Door Inspect for obstructions.
Error Occurred does not reach open Verify alignments.
While Opening position when
opening Replace sensors if
necessary.
B44425AC 7-17
Troubleshooting
7-18 B44425AC
CHAPTER 8
Parts Lists
The Parts Lists originally contained in Chapter 8 were updated and published as supplemental
information in the following publication:
PN B93296
DxH 500 Series, Parts Lists
B44425AC 8-1
Parts Lists
8-2 B44425AC
APPENDIX A
Quick Reference Information
A.1 Tolerances and Limits
Tolerances and limits needed for troubleshooting and servicing the DxH 500/520 system can be
found in the DxH 500 IFU manual (PN B95837), Chapter 1, System Overview, Performance Specifications
and the DxH 520 IFU manual (PN B85528), Chapter 1, System Overview, Performance Specifications These
include Background Limits, Carryover Limits and Repeatability Limits (Whole Blood CBC and Diff).
B44425AC A-1
Quick Reference Information
A.1 Tolerances and Limits
A-2 B44425AC
APPENDIX B
Specifications
B.1 Torque Specifications
This section provides the torque specifications and Torx size needed for proper installation of
components on the DxH 500/520 system. Part numbers for the torque tools and Torx tips are listed
under Tools in the publication, DxH 500/520 Parts Lists, PN B93296.
Assembly Torque Tool

RBC APERTURE HOLDER 35 cNm TORX T08
50 in-oz
WBC BATH / OPTICAL BENCH (4) 50 cNm TORX T10
RBC BATH (4) 71 in-oz
BATH BOTTOM CAP (2) 35 cNm TORX T08
50 in-oz
ELECTROVALVE - BODY (4) 40 cNm TORX T06
57 in-oz
ELECTROVALVE - SOLENOID (4) 25 cNm TORX T06
35 in-oz
HGB LED HOLDER 45 cNm TORX T08
64 in-oz
SYRINGE MOTOR (2) 100 cNm TORX T10
142 in-oz
TOP SCREWS (4) 60 cNm TORX T20
SYRINGE BODY 85 in-oz
BOTTOM SCREWS (2) 35 cNm TORX T20
50 in-oz
SYRINGE SENSOR 80 cNm TORX T10
113 in-oz
B44425AC B-1
Specifications
B.1 Torque Specifications
B-2 B44425AC
APPENDIX C
Fluidic Parameters, Analysis Cycles, Tubing List
C.1 Fluidic Parameters
see Measurands and their Derivations (Principles of Operation)
B44425AC C-1
Fluidic Parameters, Analysis Cycles, Tubing List
C.1 Fluidic Parameters
C-2 B44425AC
Appendix
C.2 Analysis Cycles - Sequence Description C
C.2 Analysis Cycles - Sequence Description
DxH 500 Timing Chart
B44425AC C-3
Appendix
C.2 Analysis Cycles - Sequence Description
DxH 520 Timing Chart
C-4 B44425AC
Appendix
C.3 Tubing List C
C.3 Tubing List
B44425AC C-5
Appendix
C.3 Tubing List
C-6 B44425AC
Verification Matrix
D
Verification Matrix
APPENDIX D
B44425AC D-1
Verification Matrix
D.1 DxH 500 Series Post-Procedure - Verification Matrix D
D.1 DxH 500 Series Post-Procedure - Verification Matrix
B44425AC D-2
Index
A coincidence corrections, 2-10

accessory box, 3-5 connect power cord, 3-16
Adjust Others, 7-8 connecting bar-code scanner, 3-15
Adjust WBC, 7-7 connecting power supply, 3-16
adjusting HGB, 3-41 connecting printer, 3-15
adjusting Optic LED, 4-33 connecting waste line to sink, 3-8
adjusting RBC Gain, 4-36 connecting waste line to waste container, 3-7
adjusting WBC/ALL Gain, 4-33 connectors (rear panel) - bar-code scanner,
ALL Mean Channel, 4-33 printer, power supply, 3-15
altitude, 3-2 connectors/ cables, 2-30
ambient temperature, operational, 3-2 consumption, power, 3-2
analysis cycle, timing diagram DxH 500, C-3 Controls, 3-42
analysis cycle, timing diagram DxH 520, C-4 Coulter Principle, 2-9, 2-19
aperture sensing system, 2-27 counting, 2-27
counting manifold replacement, 4-85
counting module, 2-23
B
backup/ restore, 4-3
bar-code scanner, connecting, 3-15
D
bath filter, cleaning WBC, 3-37 daily checks, specifications, 3-35
battery, 2-30 data acquisition, 2-15
bleaching, 5-1 date/ time setup, 3-31
Blood Sampling - fluidic diagram, 6-29 Diagnostics menu, 7-1
Diagnostics-Technician menu, 7-3
Diff Flags, 7-6
C Diff Thresholds, 7-5
Calibration, 3-42 differential measurement, 2-14
California Proposition 65, 1-12 diluent container, 3-12
carton, instrument, 3-2 Diluent Line - fluidic diagram, 6-13
caution label, China RoHS, 1-12 diluent setup, 3-23
CBC Thresholds Flags, 7-4 diluent transfer cycle, 2-25
China RoHS caution label, 1-12 diluent, connecting, 3-11
China RoHS environmental label, 1-12 diluter system, overview, 2-19
Cleaner Line - fluidic diagram, 6-19 display module assembly replacement, 4-29
cleaner setup, 3-24 disposal of electrical instrumentation, 1-10
cleaner transfer cycle, 2-25 disposal of waste, 1-11
cleaner, connecting, 3-13 door interlock, 4-2
cleaning schedule, 5-1 doors and covers removal, 4-5
B44425AB Index-1
Index
Drain Baths - fluidic diagram, 6-21 instrument carton, 3-2

drain valve module, 2-23 instrument, power up, 3-19
drain/mixing valve manifold replacement, 4-89 interface board, 2-31
drainage (waste) requirements, 3-3 interface board replacement, 4-27
DxH 500 - fluidic diagram, 6-7
DxH 500 Accessories, 3-5
DxH 500 Analyzer System, 2-1 L
DxH 500 Analyzer, functional description, 2-4 LCD removal, 4-14
DxH 500 Analyzer, summary, 2-1 lead screw (syringe motor) lubrication, 5-1
left-side door removal, 4-5
lubrication, lead screw (syringe motor), 5-1
E Lyse Line - fluidic diagram, 6-17
electrical lyse setup, 3-24
requirements, 3-1 lyse transfer cycle, 2-25
environmental label, China RoHS, 1-12 lyse, connecting, 3-12
Error Message List, 7-13
M
F main components, 2-19
filter, cleaning WBC bath (DxH 520 only), 3-37 main modules, 2-22
fluidic parameters, C-1 main screen, 3-20
front cover assembly/ components mainboard, 2-29
removal, 4-11 mainboard installation, 4-26
front cover removal, 4-11 mainboard removal, 4-24
functional description, DxH 500 Analyzer, 2-4 mainboard replacement, 4-23
manual organization, 1-2
measurands/ derivation, 2-16
G Measurement - fluidic diagram, 6-11
Gaussian impedance pulses, 2-13 mixing bubbles, 2-27
General Troubleshooting, table, 7-11
ground cable (syringe assembly), 4-94
ground wire, black (RBC chamber), 4-75 O
ground wire, black (WBC chamber), 4-79 ON/OFF board removal, 4-16
guidelines for servicing, 4-1 operational ambient temperature, 3-2
operational hazards, 1-9
Optic LED adjustment, 4-31
H o-ring, red fitting - RBC chamber (counting/
hazards, operational, 1-9 baths manifold), 4-75
heater assembly removal, 4-39 o-ring, red fitting - WBC chamber (counting/
HGB adjustment, 3-41 baths manifold), 4-79
HGB parameter, 2-28 o-rings (syringe assembly), 4-91
horizontal probe movement, 2-20 o-rings, valve, 4-88
humidity, 3-2
hydraulic / pneumatic system, 2-22
P
PMI (Preventive Maintenance Inspection), 5-3
I pneumatics system, 2-26
impedance measurements, 2-9 power consumption, 3-2
inspecting instrument, 3-5 power cord, connect, 3-16
installation parts, 3-5 power distribution, 6-3
installation supplies, 3-3
Index-2 B44425AB
Index
power supply, 2-29 replacing syringe sensor, 4-95

power supply replacement, 4-21 replacing syringe valves manifold, 4-97
power supply, connecting, 3-16 replacing tubing, 4-99
power up instrument, 3-19 replacing WBC chamber/Optics bench, 4-77
power up/power down, 4-2 requirements, electrical, 3-1
pressure, 2-26 right-side door removal, 4-6
principles of operation, 2-9 rinsing head, 2-21
printer driver, 3-27 rinsing head o-ring replacement, 4-43
printer name, 3-25 rocker module, 2-23
printer setup, 3-25 rocker motor replacement, 4-55
printer, connecting, 3-15 rocker sensor replacement, 4-59
printer, default, 3-28
printer, select, 3-29
probe belt/carriage replacement, 4-67, 4-71 S
probe motor replacement, 4-61 safety precautions, biological, 1-8
probe movement, horizontal, 2-20 safety precautions, electronic, 1-8
probe movement, vertical, 2-21 safety precautions, special, 1-7
probe sensor replacement, 4-63, 4-65 safety precautions, troubleshooting, 1-9
probe, clean/ dry (valves), 2-21, 2-22 Sample Mixing - fluidic diagram, 6-23
sample module, movement, 2-20
sample probe, 2-21
Q sampling probe replacement, 4-41
Q7 board, 2-30 sampling/rocker assembly removal, 4-47, 4-51
scatterplot, WBC, 2-13
serial number, instrument, 3-6
R set up cleaner, 3-24
RBC and PLT histograms, 2-11 set up date/ time, 3-31
RBC counting bath replacement, 4-73 set up diluent, 3-23
RBC Gain, adjustment, 4-36 set up lyse, 3-24
reagent compartment, 3-13 set up printer, 3-25
reagent plate removal, 4-8, 4-9 set up reagent, 3-23
reagent setup, 3-23 set up, waste, 3-21
reagent transfer cycles, 2-25 solenoid valve module, 2-23
removing heater assembly, 4-39 solenoid valve replacement, 4-87
removing sample/rocker assembly, 4-47, 4-51 space and accessibility requirements, 3-3
Repeatability, 3-42 specifications, daily checks, 3-35
replacement schedule, 5-1 spectrophotometry, 2-9
replacing counting manifold, 4-85 start analysis plate/ switch removal, 4-17
replacing drain/mixing valve manifold, 4-89 supplies, installation, 3-3
replacing probe belt/carriage, 4-67, 4-71 syringe assembly, 2-23
replacing probe motor, 4-61 syringe assembly replacement, 4-93
replacing probe sensor, 4-63, 4-65 syringe body/pistons replacement, 4-91
replacing RBC counting bath, 4-73 syringe sensor replacement, 4-95
replacing rinsing head o-ring, 4-43 syringe valves manifold replacement, 4-97
replacing rocker motor, 4-55 system configuration, 3-37
replacing rocker sensor, 4-59
replacing sample probe, 4-41
replacing solenoid valve, 4-87 T
replacing syringe assembly, 4-93 target value, Axial Light Loss (ALL), 4-31
replacing syringe body/pistons, 4-91 temperature
B44425AB Index-3
Index
operational ambient, 3-2

test points, 6-33
tolerances and limits, A-1
top cover removal, 4-7
torque specifications, B-1
transfer cycle, cleaner, 2-25
transfer cycle, diluent, 2-25
transfer cycle, lyse, 2-25
transfer cycle, waste, 2-25
troubleshooting, safety precautions, 1-9
tubing list, C-5
tubing replacement, 4-99
U
unpacking Instrument, 3-5
USB board (front) removal, 4-15
V
vacuum, 2-26
Valves Test menu, 7-2
vertical probe movement, 2-21
voting and averaging, 2-11
W
waste container, 3-7, 3-21
Waste Draining - fluidic diagram, 6-25
waste line to sink, connection, 3-8
waste line to waste container, connection, 3-7
waste setup, 3-21
waste transfer cycle, 2-25
WBC
bath filter (DxH 520 only), cleaning, 3-37
WBC chamber/Optic bench replacement, 4-77
WBC classifications, 2-13
WBC Mean Channel, 4-33
WBC optical / impedance measurement
technology, 2-12
WBC Scatterplot, 2-13
WBC/All adjustment, 4-33
WBC/ALL Gain adjustment, 4-33
Index-4 B44425AB
Glossary
µ — micron alert — A fault condition classification for

events occurring on the system. An alert
µL — microliter occurs when a condition exists on the
system for which corrective actions must be
µm — micrometer
taken in order for specimen results to be
A — ampere reported. This condition has no immediate
effect on the system operation as the system
absolute count — Concentration of a cell does not stop. The system alerts the
type expressed as a number per volume of operator by triggering visual alarms, and if
whole blood. applicable, audible alarms. Alerts are not
logged to the Event Log. All alerts require
accuracy — A measurement of the ability for operator review; however, the method of
the instrument to produce a test result review is specific to the individual event.
matching a known reference value.
analytical measuring range — Analytical
accurate — The reported measurement is in measuring range is the range of values over
agreement, within acceptable limits, of the which the acceptability criteria for the
preferred reference standard. Sometimes method are defined.
specified as the difference of the means of a
sample to the assay or expected value (mean analyze — To process a sample to determine
difference) or the percent difference of the the results for a test or tests.
means of a sample to the assay or expected
value (percent mean difference). anticoagulant — A substance added to blood
to prevent clotting.
action limits — The limits for a test value,
such that if the value is outside of the limits, aperture — An opening of a specific size and
some future action or review is suggested length through which cells pass for
(for example, repeat test, review blood counting and sizing.
smear, etc.).
application software — Software that
administrator — Someone whose job is to controls and implements the system.
administer the affairs of a business or
archiving — The process of removing
organization. See Operator (Level II).
inactive results from the system and storing
advanced level user — An operator who has the results in a format so that the archived
given authority beyond that of a basic data can be retrieved and viewed via the
operator. system software (and preferably also read
by an external program) at a future date,
providing a view similar to the one available
for an active or inactive result.
assay values — Values established for a

control or calibrator by repeat testing of
that material.
B44425AC Glossary-1
Glossary
audit log — A general record showing any calibration factor — A numerical factor
changes that the operators have made on applied to a result determined by an
the system, including configuration, instrument, in order to establish an
patient, and QC. Data in the Audit log is kept agreement between the instrument’s
up to a period of 2 years after which the measurement and a reference value.
system will “roll over” the log data.
carryover — The amount, in percent, of
audit trail — A record of a sequence of sample remaining in the system and picked
events, as actions performed by the up by the next sample cycled. Low-to-high
operator, from which a history can be carryover is the amount of sample with low
reconstructed for a specific area of the cell concentrations carried over to samples
system. For example, a patient audit trail with high cell concentration, such as diluent
will have changes related to patient data. to blood. High-to-low carryover is the
Audit trails are maintained for as long as the amount of samples with high cell
data is maintained on the system. concentrations carried over to samples with
low cell concentrations, such as blood to
available tests — All the tests which an diluent.
instrument is capable of performing.
CBC — complete blood count
background count — Measure of the amount
of electrical or particle interference. characters — All letters A-Z and numbers 0-9.
backup — To store data separately from the cleaning agent — A detergent used to flush
active data, while leaving the active data in sample from tubing and eliminate protein
place. Backups can be done completely, buildup.
meaning that all of the selected data is
backed up, or incrementally, meaning that CLSI — Clinical and Laboratory Standards
only the changes are backed up. Institute
base test — A test that is determined for a cm — centimeter

method, directly measured by an coefficient of variation (CV or CV%) — An
instrument (for example, WBC, for a CBC expression, in percent (%), of the data
analysis), or a test that is derived from the spread (variation) as related to the mean
RBC or PLT histogram. value. CV, CV%, and coefficient of variation
basic operator — An operator with only may be used interchangeably.
limited authority to operate the system. coincidence correction — Mathematical
basophil — A mature granulocyte WBC with adjustment of cell count and size for
granules that contain heparin and coincidence error.
vasoactive compounds. The granules stain coincidence error — Errors produced in
purple-blue with Wright’s stain. counting and sizing by the presence of more
batch — A group or set of results. than one cell within the aperture sensing
area at the same time. The system senses
batch mean — The mean or average of a set these as one large cell rather than as two
of examples. distinct cells.
calibration — The procedure used to set an complete blood count (CBC) — Whole blood
instrument at a specific value or values parameters RBC, WBC, HGB, HCT, MCV,
using a reference method. MCH, MCHC, PLT, RDW, RDW-SD, and MPV.
Glossary-2 B44425AC
Glossary
computed test — A test that is calculated deciliter (dL) — A unit of volumetric

based on the results of one or more other measurement equal to 0.1 liter.
tests.
deionized water — Water freed of salt and
consumable — A component that is required some organisms by an ion-exchange
by the physical system during operation and process. This water can be used
is typically disposed of after a single use or a interchangeably with distilled water in
finite number of usages. This includes such procedures. Also referred to as DI H2O or DI
items as calibrators, controls, liquid water.
reagents, etc.
density — The number of cells in a particular
control — A substance with predetermined region, regardless of the type of cell. On
values used as a standard to verify accuracy dataplots, as more cells appear in a
of instrument results. particular region, the color of the region
gets brighter.
control file — A set of retrieved control
results and the expected results associated Diff # — Used to represent the individual
with them. Each control file contains results count tests, which includes: NE#, LY#, MO#,
from a single instrument and a single EO# and BA#.
control lot or specimen.
Diff % — Used to represent the individual
Control ID — A specimen ID that cannot be differential % tests, which includes: NE%,
used for patient specimens because a LY%, MO%, EO% and BA%.
control has been configured with a lot
number that matches the Specimen ID. differential (Diff) — Leukocyte or white
blood cell differential.
critical limits — The limits for a test value,
such that if the value is outside of the limits, discrete test — Refers to either a single base
the patient’s life may be threatened and test or a single computed test (for example,
immediate action and notification is WBC which is a base test or HCT which is a
required. computed test).
critical result — A result considered distilled water — Water freed of solids and
sufficiently abnormal as to warrant organisms by distillation. This water can be
immediate notification of the physician. used interchangeably with deionized water
in procedures.
CSV — comma-separated values
down-time — Any time the system is not
CV — coefficient of variation available for testing.
cyclic redundancy check (CRC) — A EDTA — ethylenediaminetetraacetic acid

common technique for detecting data
transmission errors. erythrocyte (red blood cell) — A biconcave
disc, 6 to 8 m, that carries oxygen to the
dataplot — A graphic representation of tissues in the body and carries carbon
results. Dataplots present a combined view dioxide away from the tissues.
of population density and membership.
Colors represent different types of cells. ethylenediaminetetraacetic acid (EDTA) —
Shades of colors represent the number of A common anticoagulant used for
cells--bright colors are the most dense. hematological testing.
dB — decibel
B44425AC Glossary-3
Glossary
event — A noteworthy occurrence; hematocrit (HCT) — Red cell packed volume.

something that needs to be logged. The percentage of packed red cells
compared to the entire blood sample.
exception — A message that indicates why a
specimen was not processed or skipped, or a hemoglobin (HGB) — A protein component
default test order or no match occurred. of red cells that carries oxygen and carbon
dioxide.
expiration date — A manufacturer’s
recommended last day of use for a reagent, hemoglobinometry — Measurement of
control, or calibrator. hemoglobin in the blood.
export — To format and store data so that it hertz (Hz) — A unit of frequency.
can be used by external programs (for
example, Microsoft Excel or Word). histogram — A graphical display of the cell
size distribution of a blood sample, where
FDA — Food and Drug Administration size is on the X-axis and frequency is on the
Y-axis.
femtoliter (fL) — Femtoliter, a unit of
volumetric measurement equal to 10-15 liter. hold — When an individual test value, panel
or set of test results is identified as requiring
final report — Any patient report dispatched further review and verification prior to
subsequent to the entire set of patient’s release.
results being final released.
Hz — hertz
five-part differential — Classifying
leukocyte cells into five sub-populations immature reticulocyte fraction (IRF) — The
(neutrophils, lymphocytes, monocytes, ratio of immature reticulocytes to the total
eosinophils and basophils). number of reticulocytes.
flagging — The ability of a system to identify imprecision — The degree to which a result
and alert the operator to the presence of will vary due to random error when
possible anomalies that may affect the measured several times on the same
accuracy of a test result or require instrument.
additional work to be performed.
in vitro — Outside of a living organism, such
flags — A flag is a single letter or symbol and as in a laboratory or in an artificial
will always appear to the right of a result. A container.
flag can be instrument generated (R, P), or
in vivo — Inside a living organism, associated
laboratory-defined (H, L, c, a). On screens
with the physiological system.
and printouts, the letters, such as H, L, and R
appear next to parameter results to indicate indices — In hematology, refers to the
specific conditions. following calculated values for red cell
properties: MCV, MCH, and MCHC.
ft — foot or feet
instrument — An analytical or preparation
gal — gallon
unit composed of one or more modules.
giant platelets — Platelets above 20 fL in
interfering substances — Components
size.
within a blood sample that complicate or
gram (g) — A unit of weight. obstruct the measurement of the desired
parameters.
Glossary-4 B44425AC
Glossary
Inter-laboratory Quality Assurance mean — Arithmetic average of a group of

Program (IQAP) — A program administered data.
by Beckman Coulter, Inc. for users of its
hematology instruments and controls. It mean cell volume (MCV) — Average volume
allows a laboratory to compare its of red blood cells expressed in fL.
performance to all other laboratories in the mean corpuscular hemoglobin (MCH) —
program that use the same or similar The weight of hemoglobin in the average
instrument category and control products. red blood cell expressed in picograms.
IQAP — Inter-Laboratory Quality Assurance mean corpuscular hemoglobin
Program concentration (MCHC) — The weight of
IVD — In vitro diagnostics hemoglobin in the packed red cell volume
expressed in g/dL or g/L.
L — liter
mean platelet volume (MPV) — Average
lab administrator — An individual who has volume of platelets expressed in fL.
responsibility for running a laboratory.
membership — The different types of cells in
leukocyte (white blood cell) — Cells that a particular region, regardless of the
defend the body against disease. number of cells. On dataplots, membership
is represented showing different types of
linearity — The ability of an instrument to cells in different colors.
accurately produce a test result over the
range of possible values for a specific meter (m) — A unit of linear measurement.
parameter.
micron (µ) — One millionth of a meter.
LIS — Laboratory Information System.
(Laboratory computer) milliliter (mL) — A unit of volumetric
measurement equal to 10-3 liter.
LIS query — When a clinical instrument
requests test information for a particular millimeter (mm) — A unit of linear
specimen from the LIS system. measurement, equal to one-thousandth of a
meter.
liter (L) — A unit of volumetric measurement.
mL — milliliter
log — A record of certain system occurrences
or events. mm — millimeter
lot number — An identifier assigned by a monocyte (MO) — A large, mononuclear,

manufacturer to identify a control, reagent phagocytic WBC found in the peripheral
or calibrator. blood and in the lymphoid system.
lymphocyte — WBC originating in the lymph mononuclear — Having only one nucleus.
system. The key to the body’s immune
mW — milliwatt
system, the lymphocyte recognizes and
eliminates foreign pathogens in the body. n — number
lyse — To break apart or dissolve. nucleated red blood cell (NRBC) —
Immature form of the red blood cell
m — meter
characterized by the presence of a nucleus.
B44425AC Glossary-5
Glossary
offline — The system is not conducting partial voteout — An individual aperture

specimen scheduling, specimen delivery, or count that is not used in the average
specimen processing. parameter value.
online — The system is conducting specimen pg — picogram

scheduling, specimen delivery, and
specimen processing. platelet (thrombocyte) — The cytoplasmic
fragments of megakaryocytes, circulating as
opacity — A transformation of the data small discs in the peripheral blood, and an
derived from the ratio of the RF and DC essential component for blood clotting.
components obtained during data
acquisition. It is calculated for every PLT — Platelet parameter
individual cell measurement or event. Plt Histogram — The portion of the Plt
Opacity has the effect of removing the size distribution curve between 0 fL and 36 fL.
component, yielding a measurement that is
more closely related to the internal contents polynuclear — Having many nuclei.
of the cell.
pounds per square inch (psi) — A unit of
open vial — The sampling of a specimen pressure measurement.
(blood) by removing the cap from the
container. precision — A measure of the ability of the
instrument to reproduce similar results
operating range — The range over which the when a sample is run repeatedly. May also
instrument displays, prints and transmits be referred to as repeatability.
results.
predilute — Dilution of a sample prior to
operating system (OS) — Operating system analysis on the analyzer.
files, libraries, drivers, and so forth,
required for running the application. preliminary report — Any patient report
dispatched prior to the entire set of patient’s
operator — An individual with authority to results being final released.
operate the system.
primary identifier — The unique identifier
Operator (Level I) — Normal operator - has that will be used by the system to positively
low level privileges to the system software. identify a patient specimen.
Operator (Level II) — Advanced operator - privilege — Permission to perform some
has medium level privileges to the system particular function, for example, enter a test
software. order or review patient results.
Operator (Level III) — Lab administrator psi — pounds per square inch
operator - has full level privileges to the
system software. QA — quality assurance
operator ID — Uniquely identifies the QC — quality control

processor of the samples.
random error — Imprecision or variance.
outlier — Control results that fall outside the
range — The difference between the highest
expected or established range.
and lowest measurement in a series.
parameter — Component of blood that the
raw data — Unanalyzed data; data not yet
instrument measures and reports.
subjected to analysis.
Glossary-6 B44425AC
Glossary
RBC histogram — An RBC distribution curve. released — The test results have been
The normal curve ranges from 36 to 360 fL. automatically or manually validated and
The display starts at 24 fL. identified as reportable outside the system’s
domain, as defined by your laboratory.
RBC histogram — An RBC distribution curve.
The normal curve ranges from 36 to 360 fL. repeatability — The closeness of agreement
The display starts at 24 fL. between the results of successive
measurements of the same substance
RDW (red distribution width) — The size carried out under the same conditions of
distribution spread of the erythrocyte measurement. Also known as
population derived from the RBC histogram. reproducibility, precision, within-run
Expressed as coefficient of variation (%). precision, within-assay, within-run, intra-
RDW-SD — The size distribution spread of the assay, and intra-run precision.
erythrocyte population derived from the report — A formatted printed and/or
RBC histogram. Expressed as a standard electronic record of compiled specimen or
deviation in fL. system data.
reagent — A substance used (as in detecting reportable range — The range over which
or measuring a component, or in preparing the instrument is accurate.
a product) because of its chemical or
biological activity. (Webster) reported — The test results have been
automatically or manually dispatched to a
red blood cell (RBC) — See erythrocyte (red user specified destination. The test results
blood cell). may or may not have been released.
red cell indices — In hematology, refers to rerun — The ability to repeat an analysis on a
the following calculated values for red cell specimen using the same test.
properties: MCV, MCH, and MCHC.
restore backup — To bring backed up data
reference range — A range of test values back into the system so that it replaces the
determined by statistical analysis of active data in the system and becomes the
specimens collected from a normal (non- active data itself.
diseased) population.
result — A numerical value or values
reflex test — A different test, panel or obtained by performing the analysis for a
specimen preparation performed as a result particular test.
of the outcome of an earlier test. Usually
performed for confirmation of initial results run — One analysis of a specimen which
or to gain a better understanding of a generates test results.
patient’s condition.
sample — A portion of a specimen taken for
rejected — A test value, panel or set of results analysis on an instrument.
has been reviewed and identified as non-
reportable, either automatically because
another panel in another run has been
selected for release, or manually because
the operator has determined that these tests
cannot be reported.
B44425AC Glossary-7
Glossary
sample volume — The volume of a specimen system administrator — An individual who

removed from a specimen container. has responsibility for administering the
Also, the volume of a specimen that is system who may also perform activities
conditioned for a specific measuring such as configuring the modules and system
function. When the volume removed from a and performing the more non-routine
specimen container exceeds the maintenance activities.
conditioning requirement and a portion is system identifier (SID) — An identifier
discarded, or when portions of the sample entered at installation time and used to
are allocated to several conditioning identify the system when calling your
processes, the sample may be called an Beckman Coulter Representative.
intermediate sample volume.
systematic error — The bias or deviation of
SDS — safety data sheets the mean from the target value.
secondary identifier — An identifier not test — Individual parameter for which an
configured to be the primary identifier, that instrument can determine a value, either
can be used by the system to identify a directly measured or computed.
patient specimen in cases where the
primary identifier cannot be read. test order — A description of what tests are
to be performed on each given specimen.
sheath — A liquid which surrounds and
aligns another liquid. test panel — An aggregation of selected tests
that, when combined, provide results of
shift — Consecutive values that abruptly clinical diagnostic value, but do not
move from one level (mean) to another and necessarily share common analytical
then maintain a constant level. technologies.
Also, a work schedule within a day.
throughput — A measurement of rate at
SID — Specimen identification which an instrument can produce test
results, conventionally measured as tests
specifications — An exact statement of per hour.
particulars, especially a statement
prescribing materials, and dimensions for total voteout — A code (-----) that replaces
something to be installed. the average parameter result when there is
disagreement between the three aperture
specimen — The discrete portion of whole counts. The aperture counts for the three
blood taken for examination, study, or apertures were too far apart to give a
analysis. reliable average parameter value.
standard deviation (SD) — A measure of trend — Consecutive values that increase or
deviation from the mean. For example, a decrease gradually.
measure of the range of channel deviation
within a measurement. uninterruptible power supply (UPS) — A
device with a battery that allows limited
STAT — superior turn-around time (urgent or continued operation of an instrument or
rush, immediately) other device during a power outage.
sweep flow — A steady stream of diluent that upload — The transmission of data from the
flows behind the RBC aperture during instrument to the laboratory computer.
sensing periods to keep RBCs from swirling
back into the sensing zone. user — See basic operator.
Glossary-8 B44425AC
Glossary
user interface — The display and mechanical

devices (keyboard, mouse) used by an
operator to interact with the instrument or
instruments.
VAC — volts of alternating current

validated — The test results have been
automatically or manually reviewed and
confirmed according to laboratory
protocols.
VDC — volts of direct current
WBC differential — A determination of the

types and numbers of leukocytes found in a
blood specimen. This may be accomplished
by the instrument or by examination of a
stained blood smear.
white blood cell (WBC) — White Blood Cell

count results from the CBC analysis.
worklist — A listing of specimen analysis

status.
XB — Bull’s Moving Average. A quality

control mechanism used by hematology
instruments that monitors the stability of
the instrument by using the red cell indices
MCV, MCH and MCHC.
XM — Moving Average. A quality control

method that uses the Exponentially
Weighted Moving Average (EWMA) to
monitor the stability of the instrument
using the CBC, Diff, and Retic parameters.
B44425AC Glossary-9
Glossary
Glossary-10 B44425AC
www.beckmancoulter.com
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Service Manual

DxH 500 Series

Beckman Coulter, Inc. makes no representation that, upon

All other trademarks, service marks, products, or services

Initial Issue, PN B44425AA, 10/2015

Issue AB, /2016

Issue AC, 03/2018

Power Up/Power Down (4.1, Guidelines for Servicing Instrument)

4.26, Syringe Assembly Replacement

5.1, Maintenance Requirements

A.1, Tolerances and Limits

Alerts for Danger and Caution

Revision History, iii

CHAPTER 2: Instrument Description, 2-1

Analytical Reagents, 2-4

Connectors and Cables, 2-30

CHAPTER 3: Installation Procedures, 3-1

CHAPTER 4: Service and Repair Procedures, 4-1

Installation of Mainboard, 4-26

CHAPTER 5: Maintenance Procedures, 5-1

CHAPTER 6: Schematics, 6-1

CHAPTER 7: Troubleshooting, 7-1

Burn In, 7-9

CHAPTER 8: Parts Lists, 8-1

APPENDIX D: Verification Matrix, D-1

1.1 Manual Description

Language IFU Host Transmission RUO

Service Manual Updates

• An operator’s knowledge of the DxH 500 and DxH 520 analyzers.

To make it easier to access the information:

CHAPTER 1, Introduction, includes a brief description of this manual, a list of supporting

CHAPTER 7, Troubleshooting, contains problem descriptions, including a complete list of error

Specifications, contains torque specifications.

Signal Words and Special Headings

• Messages that appear on the System Manager screen are in italics.

1.2 Safety Precautions

Symbol Warning Condition Action

Disposal of Electrical Instrumentation

Waste Disposal Warning

China RoHS Caution Label

China RoHS Environmental Label

2.1 Introduction to the DxH 500 and DxH 520 Analyzers

The DxH 500 Series Systems

Components (DxH 500)

Components (DxH 520)

Shear Valve Module

Front Cover Assembly

Specimen Preparation (DxH 500)

Specimen Preparation (DxH 520)

User Interface and Device Interaction

2.2 Principles of Operation

• Spectrophotometry for Hemoglobin measurement.

Voting and Averaging

RBC and PLT Histograms

WBC Optical and Impedance Measurements

The main advantages are:

• Use of LED as optical source

The data is sent to the Q7 single board computer for treatment.

Measurands and their Derivation

Table 2.2-1 Measureands

Measurand Method Description

Table 2.2-1 Measureands

Measurand Method Description