Reg No- 2444 2100 310730 SESSESEES Ssh SS==S=ssS=eSeaer Eee CERTIFICATE Date: / 20 This is to certify that Mr./ Ms. Apration Ghosh Rolf No, AA. 24.003 124 Bhooes in the academic year of 20__/ 20 __ in the Institution Principal Institution Rubber StampExperiment Name/No: 4? Qualitative Oygenic Analysts [Cemiinfrsm 4) a @| Detection oP Metals s-— ciel | | The Metalic elements, if present, can be dotected by igniting the 0.S on a Platinum Spatu la. The yesidusl if any, 1s xtvacted with dil: Hel and +the solution thus | s|Obtained ts Used for thecetection of Metal ions by the Group Sebavation methods of inorganic: Gualititive analpis | lin dhe usual way. el i _ Generally, Special elements .N, 5,cl, Br | land T ave given for vouline omalysis and lassakne's fest| | Owe. icient fox the detec tion_- |@|\Delection Of Special Clements presentin cn 0.5 t- Tassaigne'’s Jest :— | j ~ ; ‘| Taking a Small piece of Freshly eut Sodium| slmnetel ond dried by pressing in belwem oa Filtre papen| meee dry and Clean’ fusion tube ond ‘place in side asrmi) piece OF Sodium _ metal. Heated the Fusion-tube untill the formation O Sodium vapour cend poured the small amount OF pouddered Organic Sample portion divectly intothe moLlter_| |Sodiem Heated the fisiontube fo redness foraboul 2 mints, and Carefully dipped Hhe fusion tube along with fusion Yesidus inka porceline basine, having distilled. cold water and Crushed Hetube. Boiled the resulting Product Ond Fiered the hot solution curd. Washed fhe residuc | with distilled water. From the Lossignes Fitvale the. Following ests ave Perboomed. Teacher SignatureLassaigne's Test :— No+ c+ N: —> Na cr 2Na+'S —>Nos Nos c+N4+s —> NaScn Noscn4 2Na—> NacN+ Nazs No+X% —> Nox (x= cl, Ber) Prussian Blue Test :— 6Nacn+ Fe $04—> Now [Fe Con) | + Now Sag 3Noaa[relen) ¢] + 4Fecls —> Fes[Fe Cone] 4 12 Nacl Reaction Pow Subbphue -— Néus+ Bb l(c ChoCOOe= PES-+ 2CK3Co0 No Mo.s+ Nag [reCen)onoj= Naa (recens Nos)" Reaction for Halagens : a NoetGbatN adl, Naclis AgNo03 = Agel vt Nanos Agel+ 2NWqoW = LAa(NW3)2 Jel + 2420 Nat Br> Nabe 5 Nain +gnos—> Ag nln, No+ = => NOx, NocT-+hgNos —> hy gigs NanogBeperenert Name / Na ‘Cami S| Experiment Obsenvatio Inference] [#[sken 2-3 mL of Lassoigne's AU - trate tn a test tube ando:I-0.29m | Aprussion blee| Nitvogen oF powdred ivon (i) sukshale. Heated | procipifale ts 15 [the mixture And at hot condition | ObLainod Present added dil H2504 4p dissolve the Iron hydvomde And add Fecls Solution . | Added few drops of dil CHsCon h2-3| Black precipible| Selphan aml of Lassaigne’s filtrate and added | is obfaitrad is few chops of lead acelle Solubion. Present @ Added 2-3 crops of freshly preparedl| Purple colour | Sulpher ditute solution of Sodum nif prusside| ts obtained. is to 2-3 ml of Lassaignes filtrale. Present )| Added few drops of dil HNos to 2-3 wl of lagsaigne’s filtrate | white/ Licht yelloo/ and add excess amount of Silver |1ellowd Colon is nibafe solution. Oblained | @) Tn white prs iota added |The white ppb iscks | Chlorine excess amount of NH40H peared ond Clear | is F Solufion is obfainal| Present @lIn light yellow Coloured ppt and add | A small amount | Bromine. excess ammount of NH40H and shaken |i5 dissolved and | _' Present} well. Htunbid solution @)| Tn yellud ppt ond exess amount | ts Oblaimed. Todine of NH40H and Shaken well |Jnsoluble in Nioq 1S Present eS ail ‘Teacher Snarefe enipinee 2 | _@-e i cet G)R- sit WD Ar-OH @)- Cor WV) Sc=0 Wi) R-ER _| _vii) Rs : _W=NB GW) NH_ Gi) N=) = E-NH Aw oe “Noy @)- CoN. ‘Detection Of Functional Groups :— __“The. tenctional Froups common encountered fn | Qatilalive jinatisis vided into two catagories. (Z) Non-Nitro genous functional Groups; Which moy bo—_| H Gili) age C or -c=#C- mn) Nik rogenous ‘hy fional TOUPS ¢ 1. Bicarbonate Jest:-A small amount of 0.5 i5 Sprinkled over Oates lion 8 Math Con toni fi 9 fs 0.5) a El a at cle aa ae beaker Care arg volumed Of Nas¢03 Solution.Sodium Metal Test :- 2 R-OH + Na ——> 2 ROPNat + Hy* Xanthale Test :- | - OH + KoH === ROK + Hho s $ Roe+ é “He oulida f \\Test Px or ALahala OH group ; Sodium Metal Test :-A [ittle 0:5 iS 3 15 dissolvec In "in-anfydrale ben- pene and a few Slee. Sodium. metal is added to it '2.| Xonthade Jeskg= Wider ge JA _LitNe ©.8 ond solid Kou (1:10) be , Warmed covle’ ef iether sue 5 ml ae iS _kaken ina cleon and dey tesk | Ie “The ame 1S aa pea 5mints. An Yellod Pet ® The 4% Nua malybdate Solution isadded, acicl:fled CHely layer hans | Violet or |__|with 2(N) S04, 2ml of che| 15 added and Shaken well. Yddish- blue@ fecls Test?— Ae 6 @)-0n-+ feclh — Ht [Fe (orh)¢ ] + 3HeL Ho-S-oW + Feclh—> O=X So P- Benzoquinone (Grawmah Greenish yellod) Back-dje Test: (Diarzo- coupling reaction) + Gor Gawd N=N Kaodye (ved) Hydvoxermic Acid Test $= _Naoi rem _g nna R~ emer ape + R-oh +10 P oe . A + BR - ti (ed yr 3Nacl NH-0/, NNH=-ONa“Test Foe Phenolic -OH Grou | Feols Tesk t- is added to Back Dye Jest: A few drops 0 of feline [5 cllSso}veal indil:Hel, Cooled in ice-walen, Paws Adhop of Feals Solution i Ue Col aquous or alcholic Solution of 0. 6 6 {i) Re in) Reddish Colas Observation [ Carin [soem 6 (violet or green ® Phenolic ov blue colourahen|-OH' uta be Hon ov ppt drops af Cold dil NaNQ Solubion . dye Fhe clear Solubion 18 added 4 cold Solubion af 0.S in NaoH. .|Test for Esters :— drovamic avi A Saturated ates Pai re 5 ee eee 4 Violek or Aomiature ofa 0-4.9m 5% anedbano fia sphelon ce ikon wine Colour Con Pi-med lene Hel, unfill amirchere me. Wem is bolle Coll and avighficd with kil Hel ano! hen 2 drops of feck ton 15 Qdleéal bo it. E ‘Teacher Signature| 2,4—DNP _tesk :- On No | ‘exo + HN. NHN, HPs Neen. NIC), Hydraxone., 6 os | Fehling’s Tesk :+ Fehling’s 1 Fehlings @ wow solukion Alkaline Solution of Sodtum_ Potossiuim Tarlanake.” CuSogt 2Naol~> Culell)+NaySo4 Hod cH— cooNal yok = Cook, Ho (Rochelte’ Sot) PH HJO- a coo Na Sr oth cook. sce 70 CHE CoO Na ran ih eS — CH= cook Deop blue eoloun « Desh blue colour _Relto_, CUx0 dj + Reook Red ppt |Expeviments ®| Test tow carbanyl Group iE |__| 24-Dinitrophenoyl, Hydrazine best | 0:1 9m OF 0.8 IS dissolved in min_ |_|quanbity of ethanol or methanol and Am of Bradys reagent (21-DNp sol") A yellow or| Se=o orange ppt | Group deed to ib.ond haken vigourously | [con Pirraadl With sevalehing dhe ime a Pl | with a. 91085 od. TP Here is n0 pel the fesl- debe is wormed ina hol x bath and allo to Tip Stil here is mo pPt a dropof Conc. 1)504 1s cudded » heated in a water balk Shaken ond ha test habe “5 Scratched coi Yass Yod. a Fehling’s Test : ‘| aa fellas solution (eeal.| Yell Zz KO a ° voup ling Land Febli aR eniecoe | red Confirmed. aoe hoctled in a waler-balh 10 ominks. pre [ii an cS el aa a a Teacher Signature 0:1.9m of 0.5 i's diss- bolved in eml oP oil Holand Cooled | Red or at 0.5% in an ice bath 1ml oF Orange - red |.qroub ice-cold Sdubion of dil NaNo, is | dye confirmed added and the mixture is added tothe icey cold alkaline Jolution of B-naphol. —Test for secondary amine :- EE Nitroso oildest op Li a 1.9m Of 05 Is diaxolised Allotoing| aoe j pees as described In the cased | Carnlin [rece 3 owt NH a Pe ryamine And gil, ainaal or ro rma is exhracfed wih CHer. The, \Greanish rae pile! extract is cried wih anhyorous re a $04, decanted hha waleh 9lassand| Colour ; fo dryness ona charm wa en to gel residual oll or Solid. Tho. Comfound is (wih 0-0 ds. oF Alva and 1. ml of Conc: 4yS04. The anrthuxe_ is Pen Poured info woaler taken p in a beaker. Tho content op the beaker alkaline . =N g Tocid)- -acelic aaa ae OF ; 15 added to 029m of 0.5 in 4ml_ | Purble Gon Prva allchol Tre mixture 1s headed | colous on a_waler— 5 mints. Teacher SignatureCamilin [age ne 1 x periment lObsenvalon [I Finference| [Test for Apilido (Substituted Amide | =CO-NHAY) Goup + Brillon E Anilido Hydralysis Test: 0-059 OF 0.s [is aesivces by 3ml. Conc Hel and ‘ ved or ye 2anl waler or 50% Syml H2504 for fed Seon le L ( {OA 9M OF 0.5 ‘aceeohlin DoD alchol, a Lille. Solid NHa4cl ar loz cael, solution and a Pinch of Zr-dusl| is added to ib. Tha minduxe \s boiled hra a minuks, Cooled cond allowed fr 5 mimutes and. Hon Pillered, Hae Piltrale| rmdd s— ) [lc tks ave Pen | Teachers SignatureCarniin [rece owe td (aga Experiment Obsan vation a @IA Portion of the Solution is A Silvan ninnor added -to the Tollen’s reagent lor block o» grey and Fon LOowmed ‘na Dalen| ppl. bath. S | Two drops OF benzoyl chhri wine ved lond 2 drops of Cone. Hol are |colourot ferie added 4 anofher Portion of hydro xeon ata. He filtrolp followed by +2 drops OF Fecls solufion- || Te lash portion of Jb Hlfrak| Read ppt. is Wormed a [ile anal add Feblinds sol” (DP and) Tes Bp anilida _groub a Hyolvelysis “Test s= 0-2 9m of 0.5 hooded wotlk \Chavacferisfic. |-conn Qeol. of 50% NooH Solution. Small of NHsduems grou mercurous nilrate loon dl Raber deep blue Teacher Signature