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path. The asymmetric material removal may displace the this, a total of sixty maxillary lateral incisors were selected.
long axis of curved root canal during shaping and cleaning. The soft-tissue residues and calcified materials were removed
Damage to apical foramen, zip formation, perforation, using scaler and were immersed in a 0.1% thymol solution
and ledging could be the possible outcomes of canal for 24 h. The teeth were then rinsed under running water to
transportation.[6] eliminate thymol residues and stored in saline until use. The
Institutional Ethical Committee approval was obtained, and
There are various methods to evaluate the efficacy
the study was completed in 7 months.
of different methods in removing root canal filling
material from the canals. These include splitting the Mounting of the samples
teeth longitudinally and visualizing them using a
To reproduce the clinical situation, the instrumentation
stereomicroscope or using Image Analyzer software,
procedures were done under a surgical operating
radiography, and digitized images.[7] Loss of residue is
microscope (G4, Global Surgical Corporation), following
usually encountered with splitting methods, thus providing
fixing of samples with putty in a maxillary jaw model
inaccurate assessment.[8] Radiographic images are two-
(ModuPro Endo, Acadental, Overland Park, KS, USA) on
dimensional (2D) representation of a 3D object and are
a phantom head.
subjected to distortion and magnification.[9]
Samples were decoronated to standardized root length of
As evident from the various literature, there are numerous
16 mm and verified using digital caliper. A small groove
methods to remove root canal filling material and to
evaluate the canal shape following retreatment procedure.[10] was marked using HiDi 501 and 720 round diamond bur
Tasmedir et al. reported significantly less root canal filling (Dentsply Ash, Weybridge, UK) at 3 (apical), 6 (middle),
material with PTUR as compared to R-Endo, Mtwo, and and 9 (coronal) mm from root apex, to facilitate the
Hedstrom files. Complete elimination of root fillings was pre- and postinstrumentation CBCT image superimposition
not noted in any of the groups.[4] Gogulnath et al. reported and analysis.
no statistically significant difference for canal centering Root canal filling
ability between PTUR and R-Endo groups.[11]
The no. 10 k-file (Dentsply Maillefer, Ballaigues, Switzerland)
Advances in imaging technology with the introduction was used to establish the canal patency. All the samples
of cone-beam computed tomography (CBCT) have led were prepared to the size A1 (size 40, 0.04 taper) Neoniti
to appraisal of the structure three-dimensionally, thus (Neolix, Châtres-la-Forêt, France) rotary file. The canals were
allowing detailed evaluation of morphologic features irrigated during instrumentation procedure with 3% sodium
without destroying the tooth sample.[5] CBCT imaging is a hypochlorite (Novo Dental Products Pvt. Ltd, Mumbai, India)
noninvasive technique for analysis of canal geometry and saline and 17% ethylenediaminetetraacetic acid (Largal Ultra,
efficiency of shaping techniques by superimposition of Septodont, Saint Maitre, France) throughout the shaping and
preinstrumentation and postinstrumentation images.[12] cleaning procedure and dried with paper points (Kerr Corp.,
Literature search reported that the hand files showed Romulus, MI) followed by obturation with the same size and
more dentin loss and were less centered in the canals as taper gutta-percha with Cold lateral compaction. GuttaFlow
compared to rotary file systems. Hence, null hypothesis was used as root canal sealer (Coltene/Whaledent, Langenau,
was considered, and the study was designed to analyze the Germany) in all the samples.
canal transportation, centering ability, and remaining root The teeth were temporized using Cavit G (3M ESPE,
filling material by ProTaper Universal retreatment system Germany) and stored at 37°C in 100% humidity for
(PTUR), R-Endo retreatment system, and hand files. 1 week to allow complete sealing of the sealer. Teeth were
radiographed in buccolingual and mesiodistal directions to
Materials and Methods assess the radiographic adequacy of root filling, using the
Permanent human maxillary lateral incisors extracted for following criteria: reaching the working length, uniform
periodontal reason with closed apices and a mean curvature radiopacity, and no voids.
of 20°–35° at the apical third as per Schneider’s method were
Cone-beam computed tomography scanning following
collected in 1-month duration. Teeth were radiographically
endodontic treatment
assessed. The teeth with canal calcification, severe canal
curvature, presence of additional canal, cracks/fractures, The teeth were removed from the jaw and mounted
and internal and external resorption were excluded from the in blocks made of polyvinyl siloxane putty wash
study. Teeth were visually inspected for fracture or cracks (Speedex; Coltène/Whaledent AG, Altstätten,
under surgical operating microscope (G4, Global Surgical Switzerland) measuring 5 cm × 5 cm to the level of their
Corporation, St. Louis, MO, USA). The sample size was cementoenamel junction in a parallel fashion for further
calculated using the results of previous study by Tasmedir. comparison. A small piece of an orthodontic wire was
Keeping a confidence interval of 95% and a power of at least placed at the corner of silicon blocks to determine the
80%, the sample size of 20 per group was kept. Following direction of scanning.
Samples were then scanned using CBCT equipment ORTHOPHOS XG 3D SYSTEM (Sirona – The Dental
ORTHOPHOS XG 3D SYSTEM (Sirona – The Dental Company) with the same parameters as discussed before.
Company) with the following parameters: 64 kVp, 8
Cone-beam computed tomography image analysis
Milli-A, and 900 projections within a full rotation.
The volume range on the object/ Field of view (FOV) Both images (scanned images immediately after shaping
corresponds to a cylinder with a diameter of approximately and cleaning and those after root canal filling material
8 cm and a height of approximately 8 cm. removal) were analyzed for the amount of residual filling
material, dentin loss, and degree of canal transportation.
The temporary restorations were removed, and 1 drop of
d-limonene (Nippon Shika Yakuhin Ltd., Tokyo, Japan) Measurement of canal volume and surface area
was used for 2 min to soften the gutta-percha and facilitate The area of the root canal and residual filling material was
easier initial penetration of retreatment files. recorded using the following equation.
Teeth were then randomly divided into three groups
(n = 20). Area % of remaining filling material
Group I (Hedstrom file group) Area of remaining filling material
= ×100
The canals were re-instrumented with Hedstrom files (H Area of canal wall
file) (Dentsply Maillefer, Ballaigues, Switzerland) of size Measurement of canal transportation
40, 35, 30, 25, and 20 in a circumferential quarter-turn The canal transportation was assessed using the Gambill
push-pull filing motion to remove gutta-percha and sealer formula, which is as follows:[13]
until WL was achieved.
T = (M1− M2) − (D1− D2).
Group II (ProTaper Universal retreatment file group)
Here, M1 and M2 represent the shortest distance between
ProTaper Universal retreatment files (Dentsply Maillefer the mesial edge of root to mesial edge of cleaned and
Ballaigues, Switzerland) were used as per manufacturer’s shaped canal and that of mesial edge of root canal from
instruction with endomotor. D1 (30 / 0.09) instrument was where the root fillings were removed, respectively.
used for coronal third, D2 (25 / 0.08) for middle, while Similarly, D1 and D2 represent the shortest distance
D3 (20 / 0.07) for apical third. between the distal edge of root to distal edge of cleaned
Group III (R-Endo retreatment file group) and shaped canal and that of distal edge of root canal from
where the root fillings were removed, respectively.
R-Endo retreatment files (Micro-Mega, Besancon, France)
were operated with a speed and torque-controlled electric From the formula described above, the following
motor (X-SMART Dentsply Maillefer) according to interpretation can be made. The value of T = 0 represents
manufacturer’s instructions. The Rm was used to create pilot no transportation, T > 0 represents T toward mesial, and
hole, followed by R1 to coronal third and R2 to two-third the T < 0 represents toward distal aspect of root canal. The
working length. The R3 was used to full length of the canal. same formula can also be applied on buccal (B) and palatal
(P) root aspect, where M will change to B or P and D will
In all the three groups, the additional finishing was done represent P or B or vice versa.
with X3 ProTaper Next rotary file (Dentsply Maillefer,
Ballaigues, Switzerland). In all the groups upon withdrawal Statistical analysis
of each instrument, adherent debris was removed from the The data were analyzed using the Statistical Package for
files and canals were irrigated with 3% sodium hypochlorite the Social Sciences (IBM SPSS Inc, chicago, IL, USA).
solution and final rinse with saline. Instruments were The P value was taken as significant when less than 0.05
discarded after being used in five root canals. Retreatment (P < 0.05). The mean and standard deviation among the groups
was deemed complete when no debris of gutta-percha/ was calculated by one-way analysis of variance, Kruskal–
sealer was visible with naked eyes on instruments surface Wallis, and Mann–Whitney U-tests, and the comparison among
after being removed from the canals. the various groups was done by post hoc Tukey’s test.
It should be noted that initial shaping and cleaning was
performed by one trained operator while the retreatment
Results
procedure was performed by another trained operator. All In the coronal third, a statistically significant difference was
the operators were having 2 years of clinical experience. noted when H file was compared with R-Endo and PTUR
[Table 1]. However, no statistically significant difference
Cone-beam computed tomography scanning following
was observed between R-Endo and PTUR [Table 1 and
retreatment procedure
Figure 1a-l]. Among all the thirds, maximum root canal
Specimens were scanned after removal of root canal filling material was observed in apical third, followed by
filling material in each group using CBCT equipment middle and coronal third in all the groups.
Contemporary Clinical Dentistry | Volume 12 | Issue 1 | January-March 2021 16
Ali, et al.: Canal transportation and dentin loss
a d g
b e h
c f i
Figure 2: Three-dimensional reconstructed axial view showing canal transportation at coronal (a, d, and g), middle (b, e, and h), and apical (c, f, and i)
third of root canals
Table 2: Comparison of the dentin loss in terms of analyzing them using analyzer software.[5] With splitting
mean±standard deviation at different levels among all technique, inaccurate assessment may occur due to loss of
the three groups residue.[6] Radiographic images are 2D representations of 3D
Group n Mean±SD object and are also subject to magnification and distortion.[8]
At 3 mm* At 6 mm** At 9 mm** Advent of CT scan enabled 3D evaluation of entire root
R‑Endo 20 0.45±0.31a 1.30±0.25a 1.41±0.37a canal system. CBCT operates at a significantly lower
PTUR 20 0.55±0.42a 1.38±0.38a 1.42±0.55a effective radiation dose as compared to CT and also
H File 20 1.01±0.33b 0.83±0.71b 0.73±0.23b enables 3D evaluation. CBCT does not require destruction
*Means one‑way ANOVA and Tukey’s tests were performed, of tooth specimen and provides detailed visualization of
**Means Kruskal‑Wallis and Mann‑Whitney U‑tests were performed. morphologic characteristics including root canal systems.[5]
Different alphabets indicate significant difference in the same column.
H file: Hedstrom files; ANOVA: Analysis of variance; PTUR: ProTaper Marfisi et al., in their study, have mentioned that none of
Universal retreatment system; SD: Standard deviation the experimental techniques guarantee complete removal
of filling materials as previously reported. The CBCT
of retreatment files. Hence, GP solvent was used as an evaluation found no significant difference between the
adjunct in removing filling material from canals.[18] instruments studied.[5]
Various techniques have been documented in the literature Gu et al. concluded that 10%–17% of canal area was
for evaluating the efficacy of root canal content removal with covered with filling material in their study.[1] Tasdemir
various aids including conventional radiography, clearing et al. also reported the same.[16] Tsesis et al. concluded
technique and digitized images, longitudinally splitting that 10%–17% of canal wall was covered with filling
the tooth and visualizing it under operating microscope material in their study.[13] Unal reported11%–27% of
or stereomicroscope, obtaining images with camera, and residual filling material, while Dall’Agnol et al. reported
loss during biomechanical preparation and retreatment Roig M. Efficacy of three different rotary files to remove gutta-
procedures, and canal transportation. CBCT is an emerging percha and Resilon from root canals. Int Endod J 2010;43:1022-8.
technology that allows for the evaluation of root canal 8. Schirrmeister JF, Meyer KM, Hermanns P, Altenburger MJ,
Wrbas KT. Effectiveness of hand and rotary instrumentation for
anatomy, assessment of root canal morphology before
removing a new synthetic polymer-based root canal obturation
and after instrumentation, measurement of 3D volume of material during retreatment. Int Endod J 2006;39:150-6.
filling material. CBCT allows for detailed visualization of 9. Ferreira JJ, Rhodes JS, Pittford TR. The efficacy of gutta-percha
morphological features without destruction of tooth. removal using Profiles. Int Endod J 2001;34:267-74.
However, the use of micro-CT would be more accurate and 10. Khedmat S, Azari A, Shamshiri AR, Fadae M, Fakhar HB. Efficacy
of ProTaper and Mtwo retreatment files in removal of gutta-percha
precise for the detailed evaluation of the root canal system. and GuttaFlow from root canals. Iran Endod J 2016;11:184-7.
Decoronation was limitation of this study. The study would 11. Gogulnath D, Rajan RM, Arathy G, Kandaswamy D.
be of more clinical relevance if it would have been done A comparative evaluation of the canal centering ability of three
on patients. rotary nickel-titanium retreatment systems in the mesio-buccal
canals of mandibular first molars using computed tomography.
Clearly further studies are needed to assess the efficacy, J Conserv Dent 2015;18:310-4.
maintenance of original canal morphology, and safety 12. Moukhtar TM, Darrag AM, Shaheen NA. Centering ability
of NiTi rotary instruments during retreatment with and canal transportation of curved root canals after using
complicated root canal anatomy. different nickel–titanium preparation systems. Tanta Dent
J 2018:15;19-26.
Conclusion 13. Saberi N, Patel S, Mannocci F. Comparison of centring ability
and transportation between four nickel titanium instrumentation
Within the limitations of this in vitro study, the result techniques by micro-computed tomography. Int Endod J
showed that none of the systems completely removed 2017;50:595-603.
the root filling. The overall dentin loss was highest when 14. American Association of Endodontists. Glossary of Endodontic
canals were instrumented with R-Endo, followed by PTUR Terms. 7th ed. Chicago: American Association of Endodontists;
and H files. In the R-Endo and H file group, higher canal 2003.
transportation was observed in middle third while least 15. Mollo A, Botti G, Goldoni NP, Randellini E, Paragliola R,
in coronal third. CBCT proved to be a more reliable, Chazine M, et al. Efficacy of two Ni-Ti systems and hand
files for removing gutta-percha from root canals. Int Endod J
noninvasive method and should be used in further studies 2012;45:1-6.
for evaluating root canal filling material using newer 16. Kfir A, Tsesis I, Yakirevich E, Matalon S, Abramovitz I. The
instruments and techniques. efficacy of five techniques for removing root filling material:
Microscopic versus radiographic evaluation. Int Endod J
Financial support and sponsorship
2012;45:35-41.
Nil. 17. Horvath SD, Altenburger MJ, Naumann M, Wolkewitz M,
Schirrmeister JF. Cleanliness of dentinal tubules following gutta-
Conflicts of interest percha removal with and without solvents: A scanning electron
microscopic study. Int Endod J 2009;42:1032-8.
There are no conflicts of interest.
18. Elis JJ, Josue M, Machado SL, Burkert Del PF. Use of
organic solvents in endodontics: A review. Clin Pesq Odontol
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