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Preparing Ballistic Gelatine
Preparing Ballistic Gelatine
Abstract
Meaningful assessment and comparisons on the injury potentials of various types of ammunition and credible forensic
reconstruction require an agreement on standard tissue simulant. Gelatine blocks are relatively inexpensive and easy to
produce and they meet most of the criteria for a good terminal ballistic simulant. This research looks into the variables of
preparing ballistic gelatine and their effects on penetration resistance. It was found out that consistent quality gelatine
blocks are easy to make and that the variables like water temperature have a far smaller effect than previously thought. Also
water acidity variances allowed by European Council directive on drinking water do not have any measurable effect. A
proposed standard method for gelatine preparation is presented together with penetration function for verification of gelatine
quality.
# 2004 Elsevier Ireland Ltd. All rights reserved.
0379-0738/$ – see front matter # 2004 Elsevier Ireland Ltd. All rights reserved.
doi:10.1016/j.forsciint.2003.11.036
92 J. Jussila / Forensic Science International 141 (2004) 91–98
affect the physical properties of gelatine. There are also is then slowly heated in water bath to þ40 8C and slowly
many more parameters that might also affect gelatine prop- stirred until all gelatine has dissolved. Five milliliter per litre
erties. In order to obtain good and reproducible results, the of propionic acid is added to inhibit microbial activity. The
conditions of production and storage must remain constant solution is poured into moulds and set into a refrigerator (7–
([1]. p. 191). 10 8C) for overnight. The blocks are then removed from
There is a lot of controversy (see [4]) on what type of moulds, wrapped tightly in plastic bags and stored in refrig-
simulant should be used for ballistic testing. Without erator at þ4 8C for at least 36 h before use. This recipe is
wishing to enter this debate in depth, I want to express followed also by Sellier and Kneubuehl [1] and Thompson
one fact which, is almost totally overlooked. The control of [8].
gelatine preparation process is entirely in the hands of the As the method is rather awkward several modifications
researcher. Any kind of gelatine can be produced at will to have appeared. Berlin [9] mixed 20% of gelatine straight
simulate virtually any kind of biological system. Any real into distilled water at 85–90 8C, used no MGI and let the
or simulated organs can be embedded [5]. This speaks solution stand in refrigerator at þ4 8C for a minimum of
strongly for the use of gelatine as a standard research 72 h. The gelatine was conditioned to þ20 8C before
simulant. use.
Standard simulants and measurements also commensu- Firearms Tactical Institute [10] recommend dissolving
rate the results and greatly facilitate possible meta-analysis 1000 g of gelatine into 6 l of hot tap water (49–60 8C) and
of combined results of several researchers. mixing well, add 5 ml of propionic acid and then three more
litres of water (49–60 8C). The filled moulds are allowed to
stand in room temperature for 4 h before placing them in
2. Review on various preparation methods refrigerator (þ4 8C) for at least 48 h.
Federal Bureau of Investigation [11] claimed to use 10%
Ballistic gelatine is usually used in 10 or 20% nominal solution into water at þ60 8C. ‘‘Foam Eater’’ is added to
concentrations. The actual concentration should be adjusted prevent foaming and instead of propionic acid cinnamon oil
i.e. calibrated for every batch of gelatine powder to give is used. The filled moulds are allowed to stand in room
precisely the desired penetration and to compensate for temperature for 4 h before placing them in refrigerator
possible manufacturing tolerances. Gelatine blocks are pre- (þ4 8C) for at least 36 h.
pared by dissolving the calibrated amount of usually 250A H.P. White Laboratories [12] follow the Fackler and
bloom gelatine powder into warm water, possibly adding Malinowski recipe for 10% gelatine with the exception that
preservative and pouring the solution into moulds where it the gelatine is not conditioned to þ4 8C but also used in 7–
first cools off and then solidifies. To avoid dehydration and 10 8C for at least 20 h. Yoganandan and Pintar [13] use the
unnecessary contamination by airborne microbes the moulds same recipe but use distilled water instead.
should be covered with plastic foil. The gelatine blocks are The first attempt to quantify the effects of different
then placed in a refrigerator for storage and cooling into a preparation parameters was done by Post and Johnson
prescribed usage temperature. Before use the gelatine block [14]. The results were somewhat inconclusive due to lack
properties are verified by shooting 4.5 mm steel pellets into of statistical confidence. Many sources quote Fackler who
them and measuring both impact velocity (Vi) and penetra- warns about detrimental effects of excessive heat [3]. The
tion depth (Lp). An accepted de facto standard [3] is that at gelatine manufacturer, Gelita, however, indicates [15] that
Vi ¼ 180 m/s the Lp should be 85 5 mm. The terms cali- gelling power does not significantly decrease after several
bration and verification are often used inaccurately in hours with a temperature of 60–80 8C.
various reports. Ragsdale and Josselson [17] used 20% gelatine at þ4 8C
The first to use gelatine as a ballistic simulant was Harvey to investigate temporary cavity formation. As both increased
[6] in the 1940’s. He used 20% concentration at þ24 8C. concentration and low temperature make gelatine stiffer it
Lewis [5] poured 6 l of water within the temperature range can be suspected that the recording sensitivity and thus
90–95 8C over 1200 g of 250A gelatine, stirred at low speed accuracy of the results were significantly impaired.
for 3 min before adding 3 ml of cinnamon oil as microbial The purpose of this research was to find out:
growth inhibitor (MGI). The solution was allowed to stand in
room temperature for 1 h without agitation and was then how consistent and homogenous gelatine could be pro-
poured into moulds that were allowed to cool in room duced using reasonable controls;
temperature overnight. The blocks were then stored at 5– whether the water temperature has significant effect;
8 8C tightly wrapped in plastic. whether water acidity have significant effect within the
The classic and often referred recipe for gelatine is that of European Council directive 98/83/EC recommendations
Fackler and Malinowski [7]. They recommend using 10% [16] for drinking water acidity;
solution i.e. for example 1000 g of gelatine with 9 l of water. whether gelatine batches differ significantly;
The gelatine powder is poured into cold (7–10 8C) water, whether increase in cooling time makes the gelatine
mixed and allowed to remain in refrigerator for 2 h. The mix stiffer; and
J. Jussila / Forensic Science International 141 (2004) 91–98 93
to define standard penetration function and recommend a upper pH limits of 6.5 and 9.5. After adjustment the 50%
standard gelatine preparation procedure. was transferred into the kettle for heating as described
below.
5. Fifty percent per weight (5 l) of cool tap water was
poured through the water purifier into a stainless steel
3. Materials and methods
kettle and heated to temperature TB, which was
calculated to produce the desired final temperature TF
3.1. Research equipment
of the total solution. As the water was approaching the
Testostor 171 temperature and humidity data logger no. temperature TB the gelatine powder was thoroughly
6102070085 mixed into the cool water in the plastic container until
Thermometer Testo 925 the mix was lumpless and smooth porridge. This allows
Water purifier Nerox NE2002UC (new) the gelatine particles to hydrate for a few minutes that is
Ph-meter pHScan2 by Eutech Instruments needed for the small 250A particles to swell [15].
Plastic container 30 l Orthex 358 made of PE-HD (max 6. As the water in the kettle reached temperature TB it was
þ65 8C) poured into the gelatine mix and thoroughly stirred to
Stainless steel kettle dissolve any lumps. The solution was then stirred with
Air rifle Daisy Power Line 856 electric paint mixer for 10 min. Foam was removed from
Copperhead 4.5 mm steel pellets (BB) the surface and the solution was poured into moulds.
Stainless steel casting moulds: 150 mm 150 mm The moulds were covered with polyethylene wrap to
400 mm prevent evaporation and to protect them from airborne
Refrigerator/freezer: Porkka 9010117. microbes and allowed to stand and solidify in room
Bullet velocity measurement: Oehler 35P proof chrono- temperature for 24 h. This lets the gelatine cool off
graph with three mod 55 screens (double measurement). slowly allowing time for any air bubbles to float to the
Accuracy scales: Mettler PJ3600 Delta Range surface before the gelatine solidifies. After that the
SPSS: statistical analysis software moulds were transferred to a refrigerator at þ4 8C for at
least 24 h for final solidification and to condition them
for proper testing temperature. For this research the
3.2. Research materials
temperatures were monitored with data logger.
250A bloom gelatine Extraco (Gelita )SG-720N charges 7. Before shooting, the gelatine block was removed from
no. 3610 and 6397. Charge 3610 was acquired in 1999 and the mould with compressed air, its internal temperature
has been stored in cool, dry and dark place. Charge 6397 was measured at the depth of 40 mm and recorded. The
was acquired in December 2002. prevailing range temperature was also recorded. The
Silicone spray: Würth 089221-C/SF specified verification shots were fired. After ballistic
Helsinki tap water with standard pH 8.6 testing the internal temperature was recorded again.
Acidity adjusting substances sulphuric acid (H2SO4 ) in
10% solution and natrium hydroxide (NaOH) in 10%
3.4. Tests
solution.
Table 1 presents the tests conducted and an inventory of
3.3. Gelatine preparation method gelatine blocks and their properties.
1. Moulds were cleaned and sprayed with silicone. Plastic
3.5. Test arrangement
container was well cleaned of any previous gelatine
residue Penetration was measured as follows (Figs. 1 and 2). A
2. Ten percent per weight (1000 g) of gelatine powder was rod was pushed into the bullet cavity until it touched the
measured. Nominal concentration was used without pellet. Depth of penetration Lp was measured from the rod
calibration because the intent of this research was to and 4 mm added to include the pellet diameter. Velocity was
look into possible effects of different variables. measured at 2.5 m and 4 m/s subtracted to correct for impact
3. Forty percent per weight (4 l) of cool tap water was velocity Vi at 5 m.
taken through the water purifier into the plastic container
and its temperature TA was measured. Water purifier was 3.6. Statistical analysis
seen as a necessary precaution in order to stabilise the
water and remove impurities and most of the microbes Variable Lp/Vi (penetration in mm divided by impact
[18]. velocity in m/s) was calculated for every shot.
4. For testing the effects of acidity all water was first Significance of difference of means was tested with
poured into the plastic container where the acidity was Mann–Whitney test for independent samples with confi-
adjusted to the EC directive [16] prescribed lower and dence level of 95%.
94 J. Jussila / Forensic Science International 141 (2004) 91–98
Table 1
Inventory of gelatine blocks and their properties
Test number Block number Charge number Water pH TA (8C) TB (8C) TF (8C) Note
Fig. 2. Three series of test shots were fired into every gelatine block from three sides (S-side, B-bottom and T-top). Each series consisted of eight
shots at different velocities achieved with 3, 6, 9, 12, 3, 6, 9, 12 pumps of the airgun. The approximate velocities were 110, 150, 170 and 190 m/s.
J. Jussila / Forensic Science International 141 (2004) 91–98 95
4. Results Table 2
Statistics of the results
4.1. Preparation monitoring Number of shots Lp/Vi SD
The solidification took place in a dark storage room. Its Minimum Maximum Mean
temperature varied between 16.7 and 18.7 8C and humidity 1 24 0.41 0.51 0.48 0.03
between 24.6 and 35.1% RH. The cooling took place in a 2 24 0.40 0.52 0.47 0.03
refrigerator with its thermostat set to þ4 8C. The internal 3 24 0.43 0.52 0.48 0.02
temperature varied between þ1.3 and 8.2 8C with mean of 5 24 0.44 0.53 0.49 0.03
3.0 and standard deviation 1.3 8C. The internal temperatures 6 24 0.40 0.51 0.47 0.03
of the gelatine blocks were measured prior to shooting at an 7/24 (h) 8 0.42 0.50 0.47 0.03
approximate depth of 40 mm. They varied between 3.6 and 7/48 (h) 8 0.40 0.50 0.46 0.04
4.4 8C. The shooting took place within 20 min and there was 7/72 (h) 8 0.39 0.49 0.45 0.03
8 24 0.42 0.52 0.48 0.03
no measurable change in the internal temperature. The
9 24 0.42 0.54 0.47 0.03
ambient temperature at the indoors shooting range varied 10 24 0.43 0.52 0.47 0.02
between 19.8 and 22.6 8C. 11 24 0.43 0.54 0.49 0.03
1, 2, 3, 7/24 (h) 80 0.40 0.52 0.48 0.03
4.2. Basic statistics
Note: The group ‘‘1, 2, 3, 7/24h’’ is the base group for assessing
effects.
Table 2 shows the basic statistics of the results
100
4,5 mm steel BB penetration Lp
90
80
min
(mm)
med
70
max
60
50
40
90 140 190
Impact velocity Vi (m/s)
that the differences in penetrations were not significant i.e. preparation process or gelatine powder. The bloom number
block homogeneity is good. might be incorrect or the gelatine powder has been exposed
to formaldehyde, which makes it insoluble to water.
4.5. The effect of cooling time This test demonstrates not only the effects but also the
great number of variables involved in preparing ballistic
After 48 h no significant change in Lp could be observed. gelatine. The repeatability test was done using four blocks of
Mann–Whitney significance was 0.163. After 72 h, however, gelatine from four separate preparation processes and thus
the significance was only 0.025 denoting significant change. gives a reliable platform for comparisons. If in any test the
This result calls for further research. properties of a gelatine block would significantly differ from
this platform we can say that the change in the variable being
4.6. The effect of water temperature tested has a significant effect even if we did not repeat the
test with several gelatine blocks.
With solution temperature TF ¼ 58:5 8C no significant There also remains the question on how long the gelatine
effect could be observed (asymp. sig: ¼ 0:835). When TF is usable after removing from the refrigerator. It depends on
was raised to over 80 8C the change seems to be significant the block size and the ambient temperature of the shooting
(asymp. sig: ¼ 0:006 > 0:05). The slope of the penetration range. It is safe, however, to say that with the block size used
function (angular coefficient), however, seems to remain in this research the shooting should take place within 30 min.
unchanged (0.614 versus 0.594 of the standard Eq. (1)). It The size of the gelatine blocks might also be standardised to
seems therefore likely that the effect of high temperature can avoid inducing any possible error due to target size [2,20]. The
be compensated with adjusting the amount of gelatine. What current size used by the author is 150 mm 150 mm
this, however, does to tensile strength and elasticity and 400 mm. This is slightly thin for high power rifle ammunition
therefore measurements other than penetration resistance is and does not easily accommodate more than two to three pistol
unknown. shots. A better size would be 200 mm 200 mm 250 mm.
It can handle all calibres and also several shots with pistol
4.7. Water acidity ammunition. Its length also better resembles that of the length
of a real wound channel.
Neither low pH of 6.5 nor high pH of 9.5 seemed to have
any significant effect on penetration (asymp. sig: ¼ 0:142and
0.217, respectively). 6. Conclusions
4.8. Differences between gelatine batches The gelatine preparation process described in the begin-
ning seems to give good, consistent results. The following
No significant difference was observed (asymp. sig: ¼ recommendations should be observed:
0:104). As there was 3 years storage time between the two Purified water should always be used to stabilise its
batches one can conclude that the properties of gelatine quality and to get rid of most of the micro-organisms.
powder do not significantly change when stored in dark, dry Every gelatine batch should be calibrated with the pre-
and formaldehyde free room temperature. paration process to achieve the required penetration.
Temperature is not crucial, but extreme temperatures
4.9. Other results and observations should be avoided.
Water acidity variations within EU recommendations do
Despite vigorous stirring no air bubbles were trapped in not affect gelatine properties.
the gelatine blocks. This may be due to the fairly low Gelatine powder can be stored for a long time in dark, dry
gelatine concentration (10%) and the slow cooling of the and formaldehyde free conditions without measurable
solution. No significant change of penetration resistance has loss of the gelling properties.
been observed during the first 40 min at ambient temperature Penetration should be verified with two shots at different
of approximately 22–24 8C. velocities to ascertain both penetration and slope of the
penetration curve i.e. response to events of various speeds.
The preparation method presented produces homogenous
5. Discussion gelatine blocks of consistent quality.
The minimum and maximum acceptable penetrations [5] R.H. Lewis, M.A. Clark, K.J. O’Connell, Preparation of
(mm) for different pellet impact velocities (m/s) are given gelatine blocks containing tissue samples for use in ballistics
in Table 3. research, Am. J. Foren. Med. Path. 3 (2) (1982).
[6] E.N. Harvey, J.H. Butler, E.G. McMillen et al. Mechanism of
wounding. in: J.B. Coates (Ed.), Wound Ballistics, US Army
7.6. Using the gelatine
Surgeon General, Washington D.C., 1962, pp. 143–235.
[7] M.L. Fackler, A. Malinowski, The wound profile: a visual
A prepared gelatine block can be stored in þ4 8C for 2 method for quantifying gunshot wound components, J.
days. After that the penetration resistance may slightly Trauma 25 (6) (1985) 522–529.
increase, but will not make the gelatine blocks unusable [8] E. Thompson, Ordnance gelatine testing procedures, AFTE J.
provided that the penetration requirements are met. If longer 25 (2) (1993).
storage time is required, microbial growth inhibitor should [9] R.H. Berlin, B. Janzon, B. Rybeck, J. Sandegård, T. Seeman,
be used. Local effects of assault rifle bullets in live tissues. Part II.
The internal temperature of a 150 mm 150 mm Further studies in live tissues and relations to some simulant
400 mm block at the depth of 40 mm rises under 1 8C during media, National Defence Research Institute, Tumba and the
Department of Surgery II, University of Göteborg, Göteborg,
the first half hour in room temperature. The test shooting
Sweden, 1983.
shall take place within this period of time or when the [10] Firearms Tactical Institute, A simplified 10% ordnance
internal temperature of the gelatine block at the depth of gelatin preparation and test procedure, Firearms Tactical
40 mm is less than 5 8C. Institute, Tactical Briefs, April 2000.
Multiple shots can be fired into one gelatine block. The [11] Ballistic gelatine mixing procedures practised by the FBI,
radial fissures of the bullet channel must, however, neither extracted from the Internet address http://www.vyse.co-
intersect nor reach to the block surface. mon31.3.2003.
[12] H.P. White Laboratories, Test Procedure, Ballistic Gelatine
Blocks, HPW-TP-0601.02, May 1998.
Acknowledgements [13] N. Yoganandan, F.A. Pintar, Biomechanics of penetrating
trauma, Crit. Rev. Biomed. Eng. 25 (6) (1997) 485–501. Review.
The author wishes to express special thanks for all their [14] S.M. Post, T.D. Johnson, A survey and evaluation of variables
in the preparation of ballistic gelatine, Wound Ballistics Rev.
help to Dr. Bo Janzon of Swedish Defence Research Agency
2 (1) (1995) 9–20.
(FOI) and Mr. Shawn Dodson of Firearms Tactical Institute. [15] Gelita web-pages, http://www.gelita.com,extracted31.3.2003.
[16] European council directive 98/83/EC of 3 November 1998 on
the quality of water intended for human consumption, Off. J.
References Eur. Commun. 5 December 1998.
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