Authorized Document

Only For Restricted

Circulation

NATURON HEALTHCARE LTD.

PLOT NO 233, DHEKU VILLEGE(KHOPOLI),TALUKA – KHALAPUR, DISTRICT – RAIGAD 410203
STANDARD OPERATING PROCEDURE
SOP No : MB/01/010 Issue Date :
PROCEDURE FOR
Revision No : 00 Effective Date :
DISPOSAL OF USED MEDIA
Department : Microbiology Review Date :

Supersedes : NA Page No : 1 of 5

CONTROLLED DOCUMENTS – NOT TO BE PHOTOCOPIED WITHOUT AUTHORISATION

1.0 OBJECTIVE:
To lay down the procedure for Standard Operating Procedure for disposal of used media.
2.0 SCOPE:
This procedure is applicable to, Microbiology department
3.0 RESPONSIBILITY:
Microbiologist.
4.0 PROCEDURE:
4.1 General instructions:
4.1.1 While handling of used Petri Plates, Pipettes and Flasks, wear
disposable surgical hand gloves.
4.2 For conical flasks, test tubes, stand, Stopper bottles
4.2.1 If the conical flask, test tube contain used media first autoclave it in
decontamination autoclave.
4.2.2 Immediately drain the media in the sink specified for washing with the
continuous flush of water till all the residues of the media are gone.
4.2.3 Dip the flasks and test tubes in a washtub filled with disinfectant
solution. 
4.2.4 Drain out the solution from glassware completely & wash these with
potable water to remove the solution. Scrub the interior & exterior
surfaces of the glassware with 2% soap solution or 0.5% labolene
using a long handle brush. 

Prepared by Checked by Authorized by

Name: Name: Name:

Date: Date: Date:
(Microbiologist) (Head Of Quality Control) (Quality Assurance)
 Authorized Document
Only For Restricted
Circulation

NATURON HEALTHCARE LTD.

PLOT NO 233, DHEKU VILLEGE(KHOPOLI),TALUKA – KHALAPUR, DISTRICT – RAIGAD 410203
STANDARD OPERATING PROCEDURE
SOP No : MB/01/010 Issue Date :
PROCEDURE FOR
Revision No : 00 Effective Date :
DISPOSAL OF USED MEDIA
Department : Microbiology Review Date :

Supersedes : NA Page No : 2 of 5

CONTROLLED DOCUMENTS – NOT TO BE PHOTOCOPIED WITHOUT AUTHORISATION

4.2.5 Drain out the soap solution and wash the glassware thoroughly with
potable water to remove the detergent completely and then rinse with
purified water. 
4.2.6 Dry the glassware in oven at 60 to 65°C. 
4.3 For Pipettes
4.3.1 Immediately after use dip the pipette in freshly prepared 2% liquid soap
or 0.5% labolene solution. 
4.3.2 Flush each pipettes with jet of potable water.
4.3.3 Remove all traces of liquid soap by flushing jet of potable water
thoroughly. 
4.3.4 Rinse the pipettes with purified water and distilled water and dry in
oven at 60 to 65°C. 
4.4 For Petri plates reusable 
4.4.1 Remove Agar from the Petri plates with a blunt knife. 
4.4.2 Collect the Agar in a stainless steel container and autoclave at 121°C, 15
lb pressure for 30 minutes. 
4.4.3 Immediately drain the media in the sink specified for washing with the
continuous flush of water till all the residues of the media are gone. 
4.4.4 Dip the plates in a washtub filled with disinfectant solution for 30
minutes. 

Prepared by Checked by Authorized by

Name: Name: Name:

Date: Date: Date:
(Microbiologist) (Head Of Quality Control) (Quality Assurance)
 Authorized Document
Only For Restricted
Circulation

NATURON HEALTHCARE LTD.

PLOT NO 233, DHEKU VILLEGE(KHOPOLI),TALUKA – KHALAPUR, DISTRICT – RAIGAD 410203
STANDARD OPERATING PROCEDURE
SOP No : MB/01/010 Issue Date :
PROCEDURE FOR
Revision No : 00 Effective Date :
DISPOSAL OF USED MEDIA
Department : Microbiology Review Date :

Supersedes : NA Page No : 3 of 5

CONTROLLED DOCUMENTS – NOT TO BE PHOTOCOPIED WITHOUT AUTHORISATION

4.4.5 Rinse the plates with tap water and dip them in a washtub containing
2% Soap solution or 0.5% labolene.
4.4.6 Scrub each plate with a brush or scrubber. 
4.4.7 Wash each plate thoroughly to remove soap with purified water. 
4.4.8 Finally rinse with purified water. Keep the plates for drying in oven at
60 to 65°C. 
4.4.9 Maintain the disposal record in register.
5.0 FREQUENCY:
Daily.

6.0 REFERENCES: Not Applicable

7.0 ABBREVIATIONS: Not Applicable

8.0 ANNEXURES: Register format for Media destruction record

Date Description of destructed media Autoclave load no. Pressure in Done by Checked
lbs/inch2 by

9.0 LIST OF DOCUMENT HOLDER:

Type of Document Department For Copy Holders

Master Copy Quality Assurance

Prepared by Checked by Authorized by

Name: Name: Name:

Date: Date: Date:
(Microbiologist) (Head Of Quality Control) (Quality Assurance)
 Authorized Document
Only For Restricted
Circulation

NATURON HEALTHCARE LTD.

PLOT NO 233, DHEKU VILLEGE(KHOPOLI),TALUKA – KHALAPUR, DISTRICT – RAIGAD 410203
STANDARD OPERATING PROCEDURE
SOP No : MB/01/010 Issue Date :
PROCEDURE FOR
Revision No : 00 Effective Date :
DISPOSAL OF USED MEDIA
Department : Microbiology Review Date :

Supersedes : NA Page No : 4 of 5

CONTROLLED DOCUMENTS – NOT TO BE PHOTOCOPIED WITHOUT AUTHORISATION

Control Copy Microbiology

10.0 HISTORY OF CHANGES

Effective Reviewed By
Revision No Reason for Review (sign & date)
Date

New SOP prepared.

00

Prepared by Checked by Authorized by

Name: Name: Name:

Date: Date: Date:
(Microbiologist) (Head Of Quality Control) (Quality Assurance)
 Authorized Document
Only For Restricted
Circulation

NATURON HEALTHCARE LTD.

PLOT NO 233, DHEKU VILLEGE(KHOPOLI),TALUKA – KHALAPUR, DISTRICT – RAIGAD 410203
STANDARD OPERATING PROCEDURE
SOP No : MB/01/010 Issue Date :
PROCEDURE FOR
Revision No : 00 Effective Date :
DISPOSAL OF USED MEDIA
Department : Microbiology Review Date :

Supersedes : NA Page No : 5 of 5

CONTROLLED DOCUMENTS – NOT TO BE PHOTOCOPIED WITHOUT AUTHORISATION

Prepared by Checked by Authorized by

Name: Name: Name:

Date: Date: Date:
(Microbiologist) (Head Of Quality Control) (Quality Assurance)