TABLE OF CONTENTS

Title Page No.

Abstract 1
Chapter One: Plant profile of Litsea glutinosa 2-4
1.1.Genus of Litsea glutinosa 3
1.2.Botany of Litsea glutinosa 3
1.3.Local name 4
1.4.General description 4
1.5.Useful parts 4
1.6.Chemical constituent 4
1.7.Medicinal uses 4
Chapter Two: Rationale of The Current Project 5-6
Chapter Three: Materials & Methods 7-19
3.1.Plant materials 8
3.2.Place and Period of study 9
3.3.Preparation of Litsea glutinosa powder 9
3.3.A.Extracting materials 9
3.3.B.Procedure of extraction 10
3.4.Materials and method for the experiment of hypoglycemic effect 11
3.4.A.Experimental animals 11
3.4.B.Appliances 11
3.4.C.Materials and Chemicals used for gavaging 12
3.5.Experimental design 12
3.6.Blood glucose meter (Glucometer): Design analysis: 14
3.7.How it works: sensors and amperometry: 14
3.8. Materials and method for the experiment of analgesic effect: 16
3.9. Principles: 16
3.10.Reagents, chemicals, and equipment for screening of analgesic activity 16
3.11. Experimental Design: 17
3.12. Procedures of vehicle, extract and standard drug sample preparation 17
3.12. A. Preparing plant extract 17
 3.12. B. Preparing dispirin solution: 17
3.12. C. Preparing 1% acetic acid solution 18
3.12. D. Routes of administration 18
3.12. E. Procedures: 19
Chapter Four: Results & Discussion 20-29
4.1.Result of hypoglycemic effect of Litsea glutinosa. MeOH extract 21
4.2.Result of analgesic effect of Litsea glutinosa MeOH extract: 25
4.3.Discussion 29
Chapter Five: Conclusion 30-31
Chapter Seven: References & Bibliography 32-34
Appendix 35-36
Abbreviation 37-40
 LIST OF FIGURES

Figure No. Title Page No.

1. Litsea Glutinosa. 3
2. Animal housing facility at UODA 9
3. Preparation of extract 10
4. Experimental animals 11
5. Glucometer 13
6. Preparation of glucose solution 13
7. Gavaging of mice 13
8. Preparation of Glibenclamide 13
9. Electrodes reaction 14
10. Test strip basic block diagram 15
11. Stop Watch 16
12. Preparing aspirin solution 18
13. Injection of 1% acetic acid in mice 18
14. Writhing of mice. 19
 LIST OF TABLE

Table No. Table name Page No.

1. Scientific Classification of Litsea glutinosa. 3
2. Pharmacological study on hypoglycemic effect of Litsea glutinosa (both 22
non-boiled and boiled fruit) in Swiss Albino mice
3. Effect of MELG on blood glucose level in hyperglycemic mice following 23
120 minutes of glucose loading.
4. Acetic acid induced analgesic effect of methanol extract of Litsea glutinosa 26
(bark) on Swiss Albino mice
5 Antinociceptive effect of MELG (bark) in the acetic acid-induced pain 27
model in mice.

LIST OF BAR DIAGRAM

Diagram No. Diagram name Page No.

1. Effect of MELG on blood glucose level in hyperglycemic mice 24
following 120 minutes of glucose loading.

2. Antinociceptive effect of crude methanol extract of Litsea 28

glutinosa bark in the acetic acid-induced pain model in mice.