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Datasheet for ABIN6574080

Insulin ELISA Kit (INS)

3  Images 14  Publications

Overview

Target: Insulin (INS)

Synonyms: ins1, xins, ins1-a, Insulin, IDDM2, ILPR, IRDN, MODY10, AA986540, Ins-2, InsII, Mody,

Mody4, proinsulin, zgc:109842

Reactivity: Mouse

Method Type: Competition ELISA

Detection Range: 123.5 pg/mL - 10000 pg/mL

Minimum Detection Limit: 123.5 pg/mL

Application: ELISA

Catalog no. Price Quantity Availability

ABIN6574080 Please check our website 96 tests Available

Insulin ELISA Kit (INS) for current price. Delivery in 10 to 12 Business Days
(Ships to: Asia)

Product Details

Purpose: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative

measurement of insulin in mouse serum, plasma, tissue homogenates, cell lysates, cell

culture supernates.

We offer validation data (WB) for the kit components. So you can be sure to order a

reliable ELISA kit product composed of high quality reagents.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Detection Method: Colorimetric

Datasheet for ABIN6574080 antibodies-online GmbH

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Product Details

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin (INS)

Cross-Reactivity (Details): No significant cross-reactivity or interference between Insulin (INS) and analogues was

observed.

Sensitivity: 48.5 pg/mL

Components: • Pre-coated, ready to use 96-well strip plate, flat buttom

• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Target Details

Target: Insulin (INS)

Abstract: INS Products

UniProt: P01326

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion,

Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus

Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy,

Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive

Regulation of fat Cell Differentiation

Application Details

Comment: Information on standard material:

The standard might be recombinant protein or natural protein, that will depend on the specific

Datasheet for ABIN6574080 antibodies-online GmbH

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Application Details

kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin

300 in the standard as preservative.

Information on reagents:

The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash

solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and

assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:

The provided antibodies and their host vary in different kits.

Sample Volume: 50 μL

Assay Time: 2h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards,

2. Add 50μL standard or sample to each well.
Then add 50μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Reagent Preparation:
1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit
will not be used up in one time, please only take out strips and reagents for present
experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 0.5 mL of Standard Diluent, kept for
10 minutes at room temperature, shake gently(not to foam). The concentration of the
standard in the stock solution is 10,000pg/mL. Please prepare 5 tubes containing
0.6 mL Standard Diluent and produce a triple dilution series according to the picture
shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of
diluted standard such as 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL,
123.5pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection

Datasheet for ABIN6574080 antibodies-online GmbH

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Application Details
Sample Preparation:
Datasheet for ABIN6574080
as of 31. Mar. 2022, Subject to change
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Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature,
shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and
Detection B before use. Dilute them to the working concentration 100-fold with Assay
Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of
deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not
dump the residual solution into the vial again.
Note:
1. Making serial dilution in the wells directly is not permitted.
2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents
at 37 °C directly.
3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the
volume errors.
4. Please carefully reconstitute Standards or working Detection Reagent A and B according to
the instruction, and avoid foaming and mix gently until the crystals are completely
dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that
pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used
only once.
6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature
and mix gently until the crystals are completely dissolved.
7. Contaminated water or container for reagent preparation will influence the detection result.
• It is recommended to use fresh samples without long storage, otherwise protein
degradation and denaturationmay occur in these samples, leading to false results. Samples
should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or
-80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the
frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to
determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a
possibilityof causing a deviation due to the introduced chemical substance.The
recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values
are not in therange of the standard curve, the optimal sample dilution for the particular
experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
antibodies-online GmbH
Schloss-Rahe-Str. 15
52072 Aachen
Germany
 Phone: +49 (0)241 95 163 153
Fax: +49 (0)241 95 163 155
E-Mail: info@antibodies-online.com
www.antibodies-online.com

Application Details

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of

target were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level

of target were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV < 10%

Inter-Assay: CV < 12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face,

and clothing protection when using this material.

Storage: 4 °C/-20 °C

Storage Comment: 1. For unopened kit: All reagents should be stored according to the labels on the vials. The
Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be
stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage
conditions. In addition, please return the unused wells to the foil pouch containing the
desiccant and seal the foil pouch with the zipper.

Expiry Date: 6 months

Publications

Product cited in: Niu, Yang, Yang: "Synthesis and anti-diabetic activity of new N,N-dimethylphenylenediamine-

derivatized nitrilotriacetic acid vanadyl complexes." in: Journal of inorganic biochemistry,

Vol. 177, pp. 291-299, 2018 (PubMed).

Chen, Yang, Nie, Song, Gu: "Effects of Bariatric Surgery on Change of Brown Adipocyte

Tissue and Energy Metabolism in Obese Mice." in: Obesity surgery, Vol. 28, Issue 3, pp.

820-830, 2018 (PubMed).

Tang, Tang, Wang, Ge, Zhu, Bi: "Insulin restores UCP3 activity and decreases energy surfeit

Datasheet for ABIN6574080 antibodies-online GmbH

as of 31. Mar. 2022, Subject to change Schloss-Rahe-Str. 15
52072 Aachen
Page 5/8 Germany
 Phone: +49 (0)241 95 163 153
Fax: +49 (0)241 95 163 155
E-Mail: info@antibodies-online.com
www.antibodies-online.com

Publications

to alleviate lipotoxicity in skeletal muscle." in: International journal of molecular medicine,

Vol. 40, Issue 6, pp. 2000-2010, 2018 (PubMed).

Fang, Zhang, Yu, Sheng, Shi, Zhu, Zhang, Bo: "Activiated galanin receptor 2 attenuates

insulin resistance in skeletal muscle of obese mice." in: Peptides, Vol. 99, pp. 92-98, 2018 (

PubMed).

Han, Zhang, Shao, Mu, Jiao, Hou, Sun: "C1q/TNF-related protein 9 improves the anti-

contractile effects of perivascular adipose tissue via the AMPK-eNOS pathway in diet-induced

obese mice." in: Clinical and experimental pharmacology & physiology, Vol. 45, Issue 1,

pp. 50-57, 2018 (PubMed).

Fang, Yu, Min, Han, Shi, Zhang, Bo: "Beneficial effect of baicalin on insulin sensitivity in

adipocytes of diet-induced obese mice." in: Diabetes research and clinical practice, Vol.

139, pp. 262-271, 2018 (PubMed).

Xiao, Lou, Lu, Yang, Liu, Liu, Qi, Li: "Ginsenoside Rg5 attenuates hepatic glucagon response

via suppression of succinate-associated HIF-1α induction in HFD-fed mice." in: Diabetologia,

Vol. 60, Issue 6, pp. 1084-1093, 2017 (PubMed).

Qi, Chen, Wang, Liu, Liu, Ke, Zheng, Lin, Zhou, Wu, Liu: "Subcutaneous liraglutide

ameliorates methylglyoxal-induced Alzheimer-like tau pathology and cognitive impairment by

modulating tau hyperphosphorylation and glycogen synthase kinase-3β." in: American

journal of translational research, Vol. 9, Issue 2, pp. 247-260, 2017 (PubMed).

Fang, Yu, Zhang, Wan, Shi, Zhu, Bo, Zhang: "Baicalin against obesity and insulin resistance

through activation of AKT/AS160/GLUT4 pathway." in: Molecular and cellular

endocrinology, Vol. 448, pp. 77-86, 2017 (PubMed).

Hatem-Vaquero, Griera, García-Jerez, Luengo, Álvarez, Rubio, Calleros, Rodríguez-Puyol,

Rodríguez-Puyol, De Frutos: "Peripheral insulin resistance in ILK-depleted mice by reduction

of GLUT4 expression." in: The Journal of endocrinology, Vol. 234, Issue 2, pp. 115-128,

2017 (PubMed).

Hou, Du, Han, Zhang, Jiao, Sun: "Irisin Regulates Heme Oxygenase-1/Adiponectin Axis in

Perivascular Adipose Tissue and Improves Endothelial Dysfunction in Diet-Induced Obese

Mice." in: Cellular physiology and biochemistry : international journal of experimental

Datasheet for ABIN6574080 antibodies-online GmbH

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52072 Aachen
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 Phone: +49 (0)241 95 163 153
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E-Mail: info@antibodies-online.com
www.antibodies-online.com

Publications

cellular physiology, biochemistry, and pharmacology, Vol. 42, Issue 2, pp. 603-614, 2017

(PubMed).

Qi, Ke, Liu, Liao, Ke, Liu, Wang, Lin, Zhou, Wu, Chen, Liu et al.: "Subcutaneous

administration of liraglutide ameliorates learning and memory impairment by modulating tau

hyperphosphorylation via the glycogen synthase kinase-3β pathway in an amyloid β protein

..." in: European journal of pharmacology, Vol. 783, pp. 23-32, 2016 (PubMed).

Hou, Liu, Han, Wang, Hou, Hou, Sun: "Irisin improves perivascular adipose tissue dysfunction

via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice." in:

Journal of molecular and cellular cardiology, Vol. 99, pp. 188-196, 2016 (PubMed).

Guo, Yu, Li, Liu, Feng, Wang, Meng, Li, Ju, Wang: "Impaired neural stem/progenitor cell

proliferation in streptozotocin-induced and spontaneous diabetic mice." in: Neuroscience

research, Vol. 68, Issue 4, pp. 329-36, 2010 (PubMed).

Images

Image 1. Typical standard curve

Datasheet for ABIN6574080 antibodies-online GmbH

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 Phone: +49 (0)241 95 163 153
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www.antibodies-online.com

Images

Image 2. Rabbit Capture antibody from the kit in ELISA

with Positive Control: Mouse serum.

Image 3. SDS-PAGE of Protein Standard from the Kit

(Highly purified E. coli-expressed recombinant mouse

INS).

Datasheet for ABIN6574080 antibodies-online GmbH

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Fax: +49 (0)241 95 163 155
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www.antibodies-online.com

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