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Staphylococcus aureus ( poster)

Poster · March 2022

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Humam Hamada Muslih

University of Anbar
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Laboratory Diagnosis
►Hematological investigation Author
●TLC ( Total Leukocytes Count )
Normal = 4000-10000 cells/mm 3 Humam Hamada Muslih
In case of infection = >10000 cells/mm 3
Osteomyelitis Periostitis
●DLC ( Differential Leukocyte Count)
Normal Neutrophil = 80%
In case of infection = >80% Features:
►Bacteriological Investigation ● Natural habitat:-Nostril and skin
Specimens: - Pus: from wound or abscess Follculitis Carbuncle ● Gram-positive
or burns ● cocci, 0.5-1.5um in diameter
●Nasal Swab: from suspected carrier ● Form irregular grapelike clusters
●Food: to diagnose staphylococcal ● Non-motile, non- sporing
intoxication ● Few strains are capsulated
●Blood: to diagnose endocarditis and Furuncle Impetigo
bacteremia Culture :
●Sputum: to diagnose lower respiratory ● on Mannitol sult agar
tract infection
►Culture and isolation
Staphylococcus aureus ● Resist drying heat9% NaCl
● Opt. Temp. For growth= 37°C
►Tube coagulase test ● Opt. pH for growth= 7.5
● Pigment 25 C On Nutrient agar
● Golden yellow
Virulence Factors
►Cellwall associated structures : Biochemical Properties:
● Capsule ● Catalase positive
● proteinA ● oxidase negative
● coagulase ● Ferment glucose, lactose, maltose, sucrose
►Extracellular toxins : and mannitol
● Enterotoxin
● TSST Drug resistance
● Exfoliatin toxin ►beta-lactam antibiotic
►Resistance to nafcillin
►Coagulase:
►S. aureus sensitive to vancomycin if :
● DNAase
•MIC less 2 micrgram/ml
● Lipase •Intermediate 4-8 micrgram/ml
● Phosphotase •Resistance more 16 micrgram/ml
● Protease ►Resist to tetracycline and erthromycin

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