En Assets MAN0593 07 ES Zetasizer Advance Guía Básica Tcm50 95452

Machine Translated by Google
ZETASIZER ADVANCE
BASIC GUIDE
ZETASIZER ADVANCE
BASIC GUIDE
Publication date: Friday, November 19, 2021

MAN0593-07-ES
DISCLAIMER
While diligent care has been used to ensure that the information in this material is
accurate, nothing contained herein may be construed as a representation or warranty as
to the accuracy, correctness or completeness of this information and we shall not be
responsible for errors contained in this document or for damages related to the use of
this material. Malvern Panalytical reserves the right to modify the content of this material
at any time and without notice.
COPYRIGHT NOTICE
© 2021 Malvern Panalytical. Malvern Panalytical. Copying or transmission of this

publication or any portion thereof without our express written permission is prohibited.
Malvern Panalytical Ltd. Malvern Panalytical BV

Grovewood Road, Malvern, Lelyweg 1, 7602 EA Almelo,
Worcestershire, WR14 1XZ, The Netherlands
United Kingdom
Phone +44 1684 892456 Phone +31 546 534 444

Fax +44 1684 892789 Fax +31 546 534 598
info@malvernpanalytical.com
www.malvernpanalytical.com
Zetasizer® and MADLS® are a registered trademark in the UK and/or other countries,
and is owned by Malvern Panalytical.
Windows® and Word is a registered trademark of the Microsoft Corporation.
Adobe® and Acrobat® are registered trademarks of Adobe Systems Incorporated.

CONTENT
Chapter 1 Introduction one
About the Zetasizer Advance Series two
About this manual 3
Cover removal 5
Information assumed 5
Where to get help 6
Chapter 2 Health and Safety 9
General security issues 10
General maintenance warnings 17
Instrument Warnings 18
Sample Handling Warnings 19
gases twenty
Chapter 3 Location Requirements twenty-one
Introduction 22
Environmental conditions 22
Space required 23
Services 24
Purge Specification 25
Services of the Autotitrator MPT-3 27
computer specifications 27
I
Chapter 4 Hardware Overview 29
System Overview 30
Team 31
Accessory Options 38
Chapter 5 Quick Start 41
Introduction 42
Start the system 42
fill the cells 43
Loading of cells in the equipment 48
Setting up a measure 51
Execution of a method 57
Analyze the results 61
Chapter 6 Maintenance 65
About maintenance procedures 66
Cleaning of cells and accessories 66
Cleaning the covers 66
Appendix A Specifications 67
specs 68
chemical compatibility 71
Appendix B Regulatory 73
CE Declaration of Conformity 74
Canadian regulatory information (for Canada only) 74
VCCI Acceptance (Japan only) 74
FCC Notice (for USA only) 75
Disposal of electrical and electronic equipment 76
II
CHAPTER 1 INTRODUCTION
About the Zetasizer Advance Series two
About this manual 3
Cover removal 5
Information assumed 5
Where to get help 6
one
About the Zetasizer Advance Series

The Zetasizer Advance Series instruments allow you to measure particle size and
zeta potential of particles or molecules in a liquid medium. The Zetasizer Advance series has
with unique technology for measurement over a wide concentration range and precise
temperature control to provide reproducible, repeatable and accurate measurements.
Depending on the options and accessories used, the system also has the ability to perform
the following functions:
l Automatic titration measurements if an MPT-3 Autotitrator is connected.
Other key parameters such as conductivity, concentration of

particles and, with the MPT-3 Autotitrator, the pH. The Zetasizer Advance Series range has
been designed to minimize user interaction and deliver excellent results.
Below are the options available for the Zetasizer Advance series:
Table 1.1 Characteristics of the Zetasizer Advance series equipment
Zetasizer Advance Series Kits
Lab Blue Pro Blue Ultra Blue Lab Red Pro Red Ultra Red
main capacity
side scatter Y N Y Y N Y
backscatter N Y Y N Y Y
Forward scatter Zeta Y Y Y Y Y Y
optics Y Y Y Y Y Y
Laser power (mW) 4 4 4 10 10 10
Characteristic
NIBS N Y Y N Y Y
MADLS N N Y N N Y
zeta measurement Y Y Y Y Y Y
Particle concentration N N N N N Y
pH titration EITHER EITHER EITHER EITHER EITHER EITHER
DDS N Y Y N Y Y
fluorescence filter N Y Y N Y Y
two
"Y" indicates that the feature is available for this product type
"N" indicates that the feature is not available for this product type
"O" indicates an optional feature.
About this manual

This manual covers the following equipment:
Table 1.2 Equipment Information
Team part number

Zetasizer Lab Blue ZSU3100
Zetasizer Pro Blue ZSU3200 (formerly ZSU5800)
Zetasizer Ultra Blue ZSU3300
Zetasizer Lab Red ZSU3105
Zetasizer Pro Red ZSU3205
Zetasizer Ultra Red ZSU3305 (formerly ZSU5700)
For more information about the features available for each device in the series
Zetasizer Advance, see: About the Zetasizer Advance series on the previous page.
3
Product Documentation Structure

This manual provides essential information needed to ensure safe and efficient operation of the
Zetasizer Advance Series. This handbook:
l Provides essential health and safety information, which all users should
read.
l Provides information about system location requirements.

l Describes the hardware components and configuration procedures of the series
Zetasizer Advance.
l Provides a brief description of the measurement process. l

Describes basic maintenance procedures.
l Provides equipment specifications.
This manual conforms to the following information structure for this product:
l Getting Started Guide: (This manual): Provides the essentials to get started, hardware
information, and health and safety information. All users must read this manual before using
the system.
l User Guide: Provides detailed information on how to perform a measurement and

use the software.
l Help: Integrated with the ZS XPLORER software, it provides detailed information on the
use of the system, video tutorials and details on all the functions of the software. l
Accessories Guide – Provides detailed information on each cell and optional accessories,
including the MPT-3 Autotitrator.
CAVEAT!
The equipment and the measured samples can be dangerous if used incorrectly. Users
! should read the health and safety information included in this guide before operating this
system.
4
Cover removal
Only Malvern Panalytical personnel (service engineers and representatives, etc.) are authorized
to perform any procedures that may require removal of covers.
CAVEAT!
! If unauthorized personnel remove the main covers, the installation warranty will be void.
instrument.
CAVEAT!
! Failure to follow these guidelines may result in exposure to hazardous voltages and laser
radiation.
Information assumed
General
The Zetasizer Advance series can be used with a variety of accessories allowing you to measure
a wide variety of samples. Some of these accessories prepare and deliver the sample to the optical
drive for measurement. For more information, see the Zetasizer Advance Accessories Guide.
Naming Convention
In this manual:
l The Zetasizer Advance series kits are referred to as such or as "the Zetasizer" or "the
team".
l Accessories are referred to by name or as "the accessory".
l The combination of the equipment, one or more accessories, and the computer is referred to as "the system
topic"
5
Software Option Selection

Software options are shown in bold and take the form of parent option-child option. For
example, Home-Measure size refers to the need to select Home and then choose a size
measure.
Where to get help

This section provides information on how to get help with your system.
help support
Please direct all queries regarding the system to your local Malvern Panalytical representative,
providing the following information:
l Model and serial number of the instrument. The serial number is displayed when
hovering over the instrument status at the bottom right of the software screen and
also on the rear panel of the instrument.
l Software version. To find it, click in the upper left corner next to Home and select
About. The software version will be displayed.
Please contact international help support if your local Malvern Pana lytical representative is
not available:
Telephone: +44 (0) 1684 891800 or email: helpdesk@malvernpanalytical.com.
If you are in the UK, call the following phone number:
+44 (0) 1684 891800 or email: cus

tomersupport.uk@malvernpanalytical.com.
If you are located in the United States, please contact the United States Help Desk if your local
Malvern Panalytical representative is not available:
Phone: +1 800 279 7297 or email: support.us@malvernpanalytical.com.
Note:
Support lines are primarily in English.
6
Website: www.malvernpanalytical.com
The Malvern Panalytical website offers a wide range of resources for customer use 24
hours a day, 7 days a week.
7
CHAPTER 2 HEALTH AND SAFETY
General security issues 10
General maintenance warnings 17
Instrument Warnings 18
Sample Handling Warnings 19
gases twenty
9
General security issues

CAVEAT!
! Use of the system in a manner not specified by Malvern Panalytical may impair the
protection provided by the system.
location requirements
The system has specific location requirements that must be met to ensure safe operation of the
instrument. See Location Requirements on page 21.
equipment location
CAVEAT!
! Do not position the equipment in such a way that the power cable, from where it comes out,
is inaccessible for disconnection.
CAVEAT!
Do not block the ventilation slots on the bottom of the computer or the fans on the
! rear panel. Restricting airflow can damage the instrument or cause overheating.
Purge Warnings (MPT-3 Autotitrator)
CAVEAT!
! If a nitrogen supply is used, the system must be located in a well-ventilated
environment. Turn off supply when not in use.
10
CAVEAT!
! If a nitrogen supply is used, local health and safety requirements must be followed.
temperature warnings
CAVEAT!
Warning triangles on the cuvette lid and heated lid indicate potentially
dangerous temperatures within the cell area. The cell area temperature range is 0°C
to 120°C.
CAVEAT!
If the temperature is above 50°C and the lid is opened, three successive beeps are
repeated with a pause between them until the temperature has dropped below 50°C.
laser safety warnings

The lasers installed in the equipment are detailed below:
l Blue labeled kits (Lab Blue, Pro Blue, and Ultra Blue) have a 4mW He-Ne 632.8nm laser.
l Red labeled equipment (Lab Red, Pro Red and Ultra Red have a 10 mW He laser
Ne of 632.8nm.
The Zetasizer optical drive is a Class 1 laser product and as such there is no exposure to laser
radiation in normal operation. The laser passes through the cell area, which is enclosed when
the cell is installed and the lid is closed. When the cell is not in place and the lid is open, a
mechanical laser shutter prevents exposure to laser radiation.
CAVEAT!
! Use of controls or adjustments, or performance of procedures other than
those specified herein, may result in hazardous radiation exposure.
eleven
laser warning labels

The following diagram shows the location of the laser warning labels; for more technical details, see
Specifications on page 68.
one two
1. CLASS 1 LASER PRODUCT

2. CAUTION -CLASS 3R LASER RADIATION WHEN OPEN. AVOID EXPOSURE TO ALHAZ.
Figure 2.1 Laser Warning Labels
Note:
Lab Red, Pro Red and Ultra Red equipment use a class 3B laser. Lab Blue, Pro Blue and
Ultra Blue devices use a class 3R laser.
12
electrical safety warnings

Take care not to spill liquid on the system covers when measuring samples.
The conduction of materials or liquids can damage the insulation and cause dangerous
conditions. If a spill occurs, turn off the power and clean before turning power back on to the
system. Users who suspect that dust or liquid has entered the
decks should call a Malvern Panalytical representative for assistance
technique.
CAVEAT!
! Never try to remove the covers; always contact a Malvern engineer
Analytical.
PAT test
If a PAT test is required, connect the ground lead to the proper ground. Malvern
Panalytical recommends that the product be PAT tested annually, or if it is suspected that
its electrical safety has been compromised.
Power cables and electrical safety

Power Cord Assembly Requirements
Power cords must meet the requirements of the country where the product is used. For more
information, contact your Malvern Panalytical representative.
General requirements
The requirements below apply to all countries:
13
l The power cord must be approved by an accredited agency responsible for

evaluation in the country where the power cord is installed. l The power cord must
have a minimum current capacity of 10 A (7 A in Japan only) and a rated voltage of 125
or 250 V AC, as required by the electrical system of each country.
l The wire area must be a minimum of 0.75mm2 or 18AWG, and the wire length
must be less than 3m.
l The power cord should be coiled to avoid; let him step on, pinch himself for him
ments placed on top, or gets wet. Pay special attention to the plug, the electrical outlet,
and the point where the cord exits the product.
CAVEAT!
! Do not use this product if the power cord is damaged. If the power cable assembly
is damaged in any way, replace it immediately.
CAVEAT!
! Do not use the power cord that you received with this product in any other product.
electrical safety information

The following notes provide guidelines for connecting the Malvern Panalytical power supply
using single and multiple extension plugs, connecting via AC adapters, and using Uninterruptible
Power Supplies (UPS).
CAVEAT!
To avoid electrical shock, plug the instrument or accessory into a properly
! grounded outlet. Never use the system without a properly connected protective
earth conductor.
The supplied power cord is equipped with a ground connection to ensure that the integrity of
the ground connection is maintained.
14
Recommendations on the use of extension cords

Follow this advice when using single or multi-prong extension plugs. These are also called
extension cords.
l Make sure the extension cord is plugged into a wall outlet and
not to another extension cord. The extension plug must be designed for grounding plugs
and plugged into a grounded wall outlet.
l Make sure the total ampere rating of the products to be plugged into the extension
cord does not exceed the ampere rating of the extension cord.
l Be careful when connecting a power cord to an extension cord. Some extension cords
may allow a plug to be inserted incorrectly.
l Improper insertion of the plug may cause permanent damage to the equipment or
accessory, as well as the risk of electric shock or fire. Make sure that the ground
connection (the plug or the pin) of the power cord is inserted into the corresponding
ground contact of the extension cord.
Recommendations on the use of AC adapters
CAVEAT!
! Do not use adapter plugs that bypass the grounding function or remove the grounding
function from the plug or adapter.
l Place the AC adapter in a ventilated place, such as a table or on the floor.

l The AC adapter may become hot during normal operation of the equipment or accessory.
Take care when handling the adapter during or immediately after use.
use.
l Only use the Malvern Panalytical supplied AC adapter approved for use with the
equipment or accessory. Using another AC adapter may affect the safety and
performance of the unit.
fifteen
Recommendations on the use of uninterruptible power

supplies (UPS)
To help protect the instrument or accessory from sudden and transient sags and swells in electrical
power, use a surge stabilizer, line conditioner, or UPS.
System transfer
Follow the guidelines in this section if the system needs to be moved.
team relocation
l Make sure the unit has been turned off for 5 minutes: If measurements were made below
room temperature, the base area below the cell area may be hot to the touch.
l Disconnect the computer and power supply before attempting to move the system.
l Always lift the computer by holding it on both short sides, with a firm grip on the metal
base plate. Never lift equipment by holding it by the covers.
CAVEAT!
! The optical drive weighs 19 kg. Use proper lifting techniques to avoid back injury.
l If you move the equipment long distances, Malvern Panalytical recommends repackaging
Pack it in its original box.
Transfer of the MPT-3 Autotitrator and the automatic

degasser
If it is necessary to move the unit, follow these guidelines:
16
l Disconnect the power supply before attempting to move the unit.
l Disconnect and drain or vent any piping that carries liquid or compressed air,
including the sample tube, before moving the unit.
l Lift the unit by holding it under the base.
CAVEAT!
! The MPT-3 Autotitrator weighs 5.3 kg and the automatic degasser weighs 2.75 kg. Adopt
proper lifting techniques to prevent back injuries.
l If you move the unit long distances, Malvern Panalytical recommends repackaging
Put it in its original box.
General maintenance warnings

CAVEAT!
! Before carrying out any maintenance, read and follow all the safety warnings indicated in
this chapter.
CAVEAT!
The system contains no internal serviceable parts. Never try to remove the covers of
! the optical bench, an accessory or a dispersion unit. Removing the covers voids the
warranty and may expose the user to hazardous laser radiation.
CAVEAT!
Failure to follow these guidelines may result in the emission of laser radiation or
! exposure to dangerous voltages. Laser radiation can be harmful to the body and can
cause permanent damage to the eyes.
17
Instrument Warnings
CAVEAT!
Before cleaning, always disconnect the unit from the power supply and the computer
! and unplug all electrical cables. Make sure the unit is completely dry before turning
it back on.
CAUTION
! After turning off the Zetasizer, you must wait at least 5 minutes before turning
the system back on.
18
Sample Handling Warnings
l Always handle all substances in accordance with COSHH (Control Of

Substances Hazardous to Health) of the United Kingdom or any local regulations regarding the
safety in the handling of samples.
l Before using any substance, review the Safety Data Sheets for
safe handling information.
l Use the instrument in a well-ventilated room, or preferably in a fume hood, if the

sample gases or dispersant are toxic or noxious.
l Use personal protective equipment as recommended in the Product Data Sheets.
Safety if toxic or hazardous samples are handled, especially during sample preparation and measurement.
l Wear suitable protective gloves when handling hazardous materials that may cause
skin irritation or damage, or may be absorbed through the skin.
l Do not smoke during measurement procedures, especially when using or storing
flammable samples.
l Do not eat or drink during measurement procedures, especially when hazardous

samples are used or stored.
l Be careful when handling glass (for example, glass cuvettes or capillaries). The
Hazardous materials can enter an injury caused by broken glass.
l Always test a new sample or dispersant to check chemical compatibility

mica before use.
l If hazardous samples are spilled, clean the system to remove all contaminants.
contaminants before performing another sample.
l Always label samples for analysis using the standard labeling of the
industry, especially if handled by a number of personnel or stored
for long periods. Clearly mark any operator hazards and related safety precautions required for material
handling
dangerous.
l Keep a record of all hazardous substances used in the system to pro

protect service and maintenance personnel.
19
l Always adopt responsible procedures for the disposal of residual samples. Most local
laws prohibit many chemicals from being disposed of by means that allow entry into
the water system. The user is advised to
seek local advice as to the means available for disposal of
chemical residues in the area of use. Consult the Safety Data Sheets.
l System surfaces can be permanently damaged if samples are spilled
about him. If a spill occurs, disconnect the system from the power source before
thoroughly cleaning up the spill. system surfaces
They can be cleaned with soapy water and a clean, lint-free cloth.
gases
CAVEAT!
Use the system in a fume hood if you use dispersants that emit hazardous gases.
! Consult Malvern Panalytical before using gas-emitting dispersants
flammable.
twenty
CHAPTER 3 REQUIREMENTS OF
LOCATION
Introduction 22
Environmental conditions 22
Space required 23
Services 24
Purge Specification 25
Services of the Autotitrator MPT-3 27
computer specifications 27
twenty-one
CHAPTER 3 LOCATION REQUIREMENTS
Introduction
This section describes the location requirements for the Zetasizer Advance Series. Make sure these requirements
are met before the Malvern Panalytical engineer arrives to install and commission the system.
Environmental conditions
The location must be:
l Away from strong light sources (such as windows)
l Away from powerful heat sources (such as radiators)
l Well ventilated (for noxious samples)
l On a horizontal, vibration-free bench designed to support the weight of the system (see Clearance
Requirement on opposite page)
Table 3.1 Unit Weight
Unit Weight
Zetasizer 19kg
MPT-3 autotitrator 5.3kg
automatic degasser 2.75kg
Store and operate the system under the following conditions:
Table 3.2 Environmental conditions
Condition Requirement
IP Rating Designed to meet IP41B

Funcionament condition
10°C to 35°C (50°F to 95°F)
Storage conditions
-20°C to 50°C (-4°F to 122°F)
Maximum humidity 80% for temperatures up to 31°C, decreasing linearly to 50%

Humidity
relative humidity at 40°C.
22
Condition Requirement
Altitude up to 2000m
Voltage fluctuations of the

Up to ±10% of nominal voltage at 100-240VAC, 50/60Hz, 4.0A
mains supply
overvoltage category II (IEC 60664)

Pollution degree 2 (BS EN 60664-1:2003)
Installation Category II (BS EN 60664-1:2003)
Besides:
l Do not obstruct power plugs as they may need to be unplugged

during an emergency.
l Avoid running electrical cables through areas where liquids may be spilled.
Space required
Provide enough space to allow easy access to all components and connections. Allow at
least 800mm above the table surface to access the storage area.
cells and accessories. The following table lists the dimensions of the components.
Table 3.3 Dimensions of each system unit
Component Wide Depth Height
Zetasizer 322mm 565mm 245mm
MPT-3 autotitrator 170mm 390mm 260mm
Automatic degasser 75 mm 250mm 130mm
computer and printer Consult the manufacturer's documentation
The following diagram indicates the recommended minimum required space for a system
typical.
23
75mm
150mm
150mm
Figure 3.1 Space Required
Services
This section describes the required services.
General information
The following services are required for each component:
Table 3.4 Receptacles Required for Each System Unit
Component outlets
Zetasizer one
MPT-3 autotitrator one
automatic degasser one
computer and printer 1 or more. Consult the manufacturer's documentation.
24
Power Specification
The main electrical supply must be clean and filtered. If necessary, use a system
Uninterruptible Power Supply (PSU) to eliminate spikes or noise.
Here are the power requirements:
Table 3.5 Power requirements for each unit in the system
Component Power Requirements
Zetasizer: Plug to PSU ~100-240VAC, 50-60Hz, 4.0A
Zetasizer: From PSU to Instrument ~24 V DC, 9.2 A, 221 W Max.
MPT-3 autotitrator ~100-240V, 50-60Hz
automatic degasser ~100-240V, 50-60Hz
CAUTION
Use only the included power supply unit and cables. The use of a
! different power supply unit renders any warranty void and may be inse
guro.
laser safety
Zetasizer instruments are Class 1 laser products and as such do not require special laser safety
considerations during normal operation. Nevertheless,
during maintenance (which must be performed by a qualified service engineer of
Malvern Panalytical), the maintenance engineer may be exposed to laser radiation
of class 3B. Therefore, Malvern Panalytical recommends that the administrative controls
recommendations of the Laser Safety Regulations be implemented.
(ISO60825-1:2014).
Purge Specification
Measuring samples at low temperatures there is a risk of condensation in the cell; this occurs when the
measurement temperature is below the dew point of ambient air
surrounding the cell being measured. This is particularly likely in humid climates.
25
The purge inlet port can be used to connect a dry air supply to the instrument, for example,
a supply with a dew point below the target temperature. This removes moisture from the air
in the immediate environment of the cell and prevents condensation. The air supply must
meet the following specification:
l Compressed air according to ISO 8573-1

l Oil = Class 1
l Water = Class 3
l Particulate matter = Class 3 l
Pressure = 100 kPa g.
The bleeder connection accepts a push-in connection for a 4mm nominal pressure hose.
CAUTION
The purge air piping supply must comply with the above specification. Failure to
! comply with this specification may cause permanent damage to the equipment and
void the warranty.
An optional purge connection kit (part number AVN2142) is available from Malvern Panalytical
if this installation is to be used.
When making a measurement with the purge option, keep in mind the following rules:
l The flow rate will be preset to 1.5 l/min at 1 bar.
l The instrument should be operated at a maximum temperature of 30°C and 70% relative humidity
when the requested temperature is below the dew point.
l When operating at relative humidity above 50%, the measurement must allow a minimum
equilibration time of four minutes to allow the temperature to stabilize.
26
Services of the Autotitrator MPT-3
Nitrogen purge specification

CAVEAT!
! A nitrogen supply should be used in a well-ventilated environment.
CAVEAT!
! If a nitrogen supply is used, local health and safety requirements must be
followed.
The MPT-3 Autotitrator has a purge fitting for connection of a nitrogen purge supply. This can
be used to cover the area directly above the sample and prevent any unwanted interaction
with the atmosphere.
If a nitrogen supply is required, it must meet the following specifications:
l The nitrogen supply must be dry, oil-free and filtered to remove any
any contaminants that may affect the sample.
l The flow rate should be set between 2 and 20 mL/min.
See the Zetasizer Advance Series Accessories Guide for more information on the MPT-3
Autotitrator.
computer specifications
Please contact Malvern Panalytical support or check the website for the recommended
computer specification. This is also provided in the Software Update Notification.
27
CHAPTER 4 OVERVIEW
HARDWARE
System Overview 30
Team 31
Accessory Options 38
29
CHAPTER 4 HARDWARE OVERVIEW
System Overview
Note:
Initially the Zetasizer is checked by trained personnel from Malvern Panalytical. In
This section describes how to reinstall the system; Usually required when
the instrument is moved or the computer is changed.
The image below shows a typical Zetasizer kit and its components. a cell is filled
with the sample and is loaded into the cell area at the top of the instrument. A computer with
Zetasizer software (ZS XPLORER) installed controls the equipment.
one two 3 4
1. Zetasizer Instrument 3. Cells

2. Cell area cover 4. Computer with ZS XPLORER
Figure 4.1 System Overview
30
Team
The following image and the corresponding table describe the components of the Zetasizer kit.
4
one
two
5
3
1.
Equipment 2. Cell Access Button – Press to open cell chamber cover 3. Status
Indicator Light – See below for more information 4. Back Panel – All equipment
connections, see Back Panel on page 33 5. Cell Chamber Lid – Opens to reveal the cell
chamber, see Cell Chamber on
page 34
6. Tubing Channel – Allows tubing from an optional accessory to enter the cell chamber.
Figure 4.2 Equipment Overview
status indicator
The status indicator light is located under the cell access button and shows the operational status
of the equipment.
The following table lists the indicator color and status and function.
31
Table 4.1 Colors and meanings of the status indicator
Indicator color and status Function

Amber: stable Indicates that the startup routine is running.
Amber: flash every 5 Indicates that the machine is in standby The machine is working
properly, but it is not connected to the computer or the software has not
seconds been started.
Green Indicates that the equipment is working properly and is ready to start a
measurement.
It occurs when the equipment is performing a measurement or a dispersion

Blue: slow pulse
standard.
A method is running, but no action is being performed. For example, this
Blue: stable light will indicate when the temperature is changing or equilibrating.
Red: fast flash followed by Indicates if the device has detected an error. The measurement will stop.
steady red Contact Malvern Panalytical Help Desk.
Green-blue: pulses The drive requires user interaction to continue.
32
back panel
On the back panel are all the connections. The following image and corresponding table
describe the rear panel components.
CAVEAT!
! Do not block the ventilation slots on the bottom of the computer or the cooling fans
on the rear panel.
4
5
6
one
two
7 8
1. Power switch 5. Ethernet connection

2. Power input plug: from source (diagnostics)
external DC power 6. USB connection (diagnostics)
3. Purge connection 7. Laser warning label
4. USB connection to computer 8. Cooling fan
Figure 4.3 Rear panel of the equipment
33
cell chamber
The cell chamber is where all the cells are inserted to carry out a measurement. The
cell chamber controls the temperature of the sample in the range of 0 °C to 120 °C.
CAVEAT!
The system can heat the cell to high temperatures. Take care when removing cells if a
measurement was performed at high temperatures. Let the cell chamber
cool before removing the cell.
Note:
If the lid is opened with a cell area temperature above 50°C, the instrument beeps
three times every few seconds to warn of high temperatures.
When the Zetasizer is initially turned on, the cell area is brought to a predetermined temperature of
25°C. This also happens if the software is closed, but the instrument is not
It has been turned off.
Press the cell access button to access the cell chamber.
The following image shows the components inside the cell chamber of the instrument.
3. 4
one
4
15mm
MAX
10m
ME
m
N
6
two
3 7
1. Cell chamber cover 5. Thermal Contact Plate Holder - Used with

2. Base of cells DTS1070 cells
3. Cell holder – contains the cell clamp, 6. Thermal lid (storage area)
electrodes and drain port 4. Cuvette fill 7. Channel for pipes 8.
level tips Drainage channel
Figure 4.4 Cell Chamber - Open
The following table describes some of the cell chamber components in more detail.
35
Table 4.2 Description of cell chamber components
Component Description
Press the cell access button to open the lid. The lid will open and allow access to
the cell chamber. When it is open, two safety interlocks are activated:
l Laser Safety Interlock: Prevents laser light from entering the laser area
lid of the
cell chamber the cell.
[one]
l Electrode voltage lockout: disables any voltage on the electrodes
of cell.
To close the lid, push it down until it locks. Measurements cannot be made unless the
lid is completely closed.
The cell base is made of an insulating material that provides protection
of the hot cell holder and, in conjunction with the heated cap, provide
base of cells temperature stability when heating and cooling the sample.
[two]
A warning label indicates that high temperatures may exist in the

cell area.
The electrodes provide voltage (voltage) for zeta potential measurements.
The voltage is immediately disconnected when the cell cover is opened.
Cell holder [3]:
electrodes Note:
The maximum working voltage on the electrodes is ±150 V (category I
measurement)
To keep the cell in the optimal position during a measurement, a

a cell clamp mechanism on the wall of the cell holder. The clamp will force against the
edge of the cell when inserted.
Cell support Note:

[3]: cell clamp
When using glass or quartz cuvettes, insert a quartz cuvette first.
disposable polystyrene in the cell holder. This ensures that the clamp
moves freely and that no sample spillage has caused the mechanism to
stick. Be sure to remove the cuvette
polystyrene before inserting the glass or quartz cuvette.
Cell support In the event of a spill within the cell area, there is a built-in drain in the
[3]: port cell holder base. Any spillage will exit into the bank area
drain under the Zetasizer.
36
Component Description
The heated lid provides increased temperature stability for size measurements when
the sample is heated and cooled. This is important when measuring at either end of the
operational temperature range. Place the thermal cap on the cell.
CAVEAT!
After removing the thermal cap, both the metal lining of the cap and the
top of the cell holder will be exposed. Care should be taken when cells are
! removed after measurement at high temperatures. It is recommended that
the cell area cool down before removal. A warning triangle is provided at the
Thermal cap [6] top of the cap.
The cell chamber has a square hole for storage of the thermal caps.
Note:
The heated lid is not compatible with the capillary cell, immersion cell, high
concentration cell, flow cell, or low volume disposable sizing cell.
access channel
A built-in channel in the cell area allows sample tubes to connect to the measurement
for flow cell tubes cell. This makes it easy to use the MPT-3 autotitrator. Refer to the MPT-3 Autotitrator
and autotitrator Connection section in the Zetasizer Advance Series Accessories Guide for precise details
on inserting cells with tubes.
MPT-3 [7]
In the event of a spill on the roof, there is a drainage channel around the outside of the cell
des channel chamber. This is hidden from view below the main deck. Any spillage will flow along the
drain to a hole at the rear of the cell area. Any spillage will exit into the bench area below
water [8]
the Zetasizer.
37
Accessory Options
A range of accessories and options, listed below, are available for the most advanced measurement
strategies. Specifications for each product are provided in
the Zetasizer Advance Series Accessories Guide.
Table 4.3 List of Zetasizer Accessories
Option part number Description
Cost-effective accessory designed to automate the

MPT-3 autotitrator ZSU1001 measure of size and zeta potential as a function of pH.
Together with the MPT-3 Autotitrator, the automatic

degassing unit continuously degasses the titrants,
improving accuracy.
of dispensing small volumes of
Automatic degasser DEG0003
valorant. This is particularly important
when titrated around pH 7, at which the
titrant will have a greater effect on pH in
compared to more extreme pH levels.
It is used for zeta potential measurements on
which users do not require the benefits
of the DTS1070 disposable cells or in which
immersion cell ZEN1002 better chemical compatibility is required.
It can also be used to measure in dispersants
with very low dielectric constants,
for example, nonpolar organic solvents.
To perform zeta potential measurements on a
High concentration cell ZEN1010 concentrated aqueous sample. The cell is ade
suitable for a wide range of conductivities.
A maintenance-free, disposable capillary cell
for size (backscatter and forward scatter) and
zeta potential measurements.
DTS1070 This cell also allows measurement with Malvern
Disposable capillary cell
Panalytical's patented diffusion barrier technique for
volumes up to a
minimum of 20 ÿl, where samples so sensitive
like proteins, they can be measured.
This cell is used for size measurements of
Size measurement cell
ZSU1002 very low volume. can hold a volume
ultra low volume
measurement up to a minimum of 3 ÿl. Offers
38
Option part number Description

an extended superior particle size range without
the need to modify conditions
of sample dispersion.
A wide range of cuvettes, including
buckets visit website disposable polystyrene, glass and quartz, are
available to the team. Check the website
of Malvern Panalytical for more details.
39
CHAPTER 5 QUICK START
Introduction 42
Start the system 42
fill the cells 43
Loading of cells in the equipment 48
Setting up a measure 51
Execution of a method 57
Analyze the results 61
41
Introduction
This section describes the entire process of taking a measurement, from turning on the system to analyzing the
results. The example given is that of the execution of a measurement of
size. Zeta potential measurements generally follow the same principles.
Note:
This section does not address how to perform titration or concentration measures
User'son
of particles. See the help system or the for more information guide
these measurement types, or for more
information on the measurement types.
standard measure.
Start the system
Using the system for the first time

Windows 10 computers ship with the operating system inactive. Follow the instructions provided by Windows at
startup to register and activate your copy of Windows.
Note:
You will need to connect to the Internet or call Microsoft to complete the process.
Turn on and start ZS XPLORER

If it is not on, perform the following steps to turn on the Zetasizer and start the
ZS XPLORER software:
1. Close the lid, turn on power at the outlet, and move the power switch to the on position on the rear of the unit.
The status indicator is
will illuminate a steady amber.
42
2. If it is not already on, turn on the computer.
3. Double click the ZS XPLORER software icon on the desktop:
Figure 5.1 ZS XPLORER desktop icon
4. Then the initialization routine will start. When the status indicator shows a solid green color,
the instrument has completed its initialization procedure.
Note:
Allow your Zetasizer to heat stabilize for 30 minutes before use. If the instrument has
been stored outside of its rated operating temperature range (10°C to 35°C), a longer
stabilization time may be required.
Note:
You can leave the Zetasizer on if the system is expected to be in regular use. We
recommend that the Zetasizer be turned off if it is not going to be used for periods of more
than 2 days.
CAUTION
! After turning off the Zetasizer, you must wait at least 5 minutes before turning the
system back on.
fill the cells

This section provides information on how to correctly fill cells with the
sample.
43
Cell preparation (folded capillary cells)

For folded capillary cells it is recommended to rinse the cell to ensure cleanliness and reduce
the risk of bubble formation. The recommended procedure requires two syringes; filtered
deionized (DI) water, and ethanol or methanol.
1. Rinse cell with ethanol to facilitate wetting.

2. Fill one of the syringes with the DI water and place it into one of the sample ports on the
the cell; the empty syringe is placed in the other port.
3. Rinse the contents of the full syringe, through the capillary, into the empty syringe, and then
rinse again.
4. Repeat this process four more times before removing the syringes and performing a rinse
end with the dispersant used for the measurement.
5. After this, the cell is ready for use.
Never attempt to clean the optical surface of the folded capillary cell as this will cause minor
scratches on the surface leading to inaccurate results.
44
Disposable capillary cell filling
Figure 5.2 Filling a disposable capillary cell
1. Invert the cell.

2. Using a syringe or pipette, slowly inject your sample into the cell and fill to a little
more than half.
3. Check that there are no bubbles trapped in the cell. If there are bubbles, gently tap
the cell to remove them.
4. Stand the cell upright and continue to inject the sample slowly until the cell
sample is on top of the electrodes.
5. Remove the syringe and insert a cell plug into each port.
6. Hold the cell near the top, away from the bottom measurement area, and enter
lower it into the cell holder until it stops. Make sure the Malvern logo is facing the front
of the computer.
Four. Five
Filling of buckets
1. Fill the PCS8501, PCS1115, or DTS0012 cuvette with sample using a pipette or syringe
as follows:
2. Tilt the bucket and allow it to fill slowly, as indicated.
Figure 5.3 Filling the bucket
Advice:
To stop bubble formation, allow the sample to flow down the inside wall of the
the bucket.
3. Make sure the sample depth is between 10mm and 15mm by placing the cuvette against
the diagram on the inside of the cell area lid, as shown below:
Max: 15mm
Min: 10mm
46
Figure 5.4 Correct Sample Depth
4. Press the lid firmly onto the cuvette as shown:
Figure 5.5 Placing the Lid on the Cuvette
47
Note:
Most cuvettes have a dot or triangle mark, as shown below. This mark should
face the front of the computer.
Figure 5.6 Front Face of the Cuvette
Loading of cells in the equipment

This section provides information on how to load the cells into the equipment correctly. See
Cell Camera on page 34 for more information on the cell camera.
Pay special attention to the type of cell you are charging. Each cell has a specific direction
in which it must be inserted into the cell chamber. The process is the following:
1. Press the cell access button to open the cell chamber lid.
2. Insert the cell. Make sure the orientation is correct.
3. If applicable, place the thermal cap over the cell.
4. Close the cell chamber lid after insertion.
48
cell size
Insert a cuvette into the instrument in the direction shown in the following illustration:
15mm
MAX
10mm
MIN
Figure 5.7 Correct Cell Insertion - Cell Size
49
Zeta cells
Insert a disposable capillary cell in the direction shown in the following illustration:
Figure 5.8 Correct cell insertion: zeta cell
Note:
The Malvern Hills logo on the disposable capillary cell should be facing the front of the
kit when inserted.
fifty
Setting up a measure
Here are the steps required to set up a measure:
l Create a new method.

l Specify individual Step or Group settings.
About methods
To execute a measure you must create a method.
The method builder allows you to create methods. A method consists of a series of steps that
together create a complete measurement process. The steps can be done as follows:
l Measurement steps: size, zeta, particle concentration and pH titration

l Group: a set of steps grouped together l
Basic steps: pause or show instruction
l Trend steps: run steps while varying another parameter, such as pH
Once you have defined all the step settings for the method, you can run it once or save it to use
again later.
Note:
If you forget to save the method at execution time, it can be extracted from the results
later.
51
Create a method
Creating a size or zeta method is fundamentally the same process, but the types
of measurement settings for each will be different.
Note:
This section shows the example of running a size measurement; for
get more detailed information, including details on how to run the valuation
pH or particle concentration measurements, refer to the help system or the Guide
of the series user Zetasizer Advance.
1. Click Measure.
Figure 5.9 Selecting the Measure tab
2. Assign an appropriate name to your sample.
Figure 5.10 Sample basic information window
3. Select the cell type from the dropdown menu, as this influences which steps are available to use.
52
Figure 5.11 Selecting a cell type
4. Select the appropriate material and dispersant for the sample. Or, to add the
yours, see the User's Guide.
5. Select an existing project to save the measurement results or create a
new project.
Note:
The data is not necessarily saved to the open project within the Analyze view. Be sure to
note which project your data is being saved to.
6. To create the method, drag or click to add steps from the side panel.
53
Figure 5.12 Selecting a step in the side panel
Note:
Some steps may be grayed out depending on the settings selected. For example,
The cell selected in the image above cannot be used for zeta potential measurements, so
the zeta measurement step is not available.
7. Define the necessary settings in the Properties area .
Advice:
You may find it useful to expand the (Properties)
Properties
area to get a
clearer vision. To do this, click the expand icon <1/> in the header of the
<2>Properties</2> area.
54
Figure 5.13 Properties window expanded
Setting the order of execution of steps

The steps are executed in the order they are listed in the method, from top to bottom. To
reorder the steps, click on the circle gripper in the image below and drag it up or down as
required:
Figure 5.14 Reorder steps
55
Repetition of steps or groups

Steps can be specified to repeat as many times as necessary; also groups. Set the number of repeats by
clicking the repeat icon on the
He passed.
In this example, the step will be executed three times.
Figure 5.15 Repeating a step 3 times
Note:
If you repeat a zeta measurement three times, the system will optimize only once. However, if
you perform three different zeta measurements, or repeat a group containing a single zeta
measurement three times, the system will optimize before each measurement, that is, three times.
56
Execution of a method
This section guides you through the process of running a method.
1. After setting up a method (see Setting up a measurement on page 51), click Start Method at the top of the
window:
Figure 5.16 Executing a method
2. The software will display the progress of the measurement.
Figure 5.17 Measurement in progress
57
3. Each step is executed in sequence. When a measurement is made, the graphs and Method List are
updated in real time.
Tip: You
can continue working in other areas of the software during the measurement; it is not necessary
to observe the measurement being performed.
Note:
Abort the measurement at any time by clicking one of the red Stop buttons. The system will
request confirmation before stopping the measurement.
4. To set up a new measurement while another is running, click the + button at the top of the window.
Figure 5.18 Setting Other Measurement
5. When the method is complete, it is displayed on the Complete tab of the Method List. It is also available
in the general method list to edit and rerun.
58
Figure 5.19 Measurements completed
Advice:
The currently executing method step is shown with a flashing icon as in sample 2 () above, in
which there is a measurement stepSample
of 2
current particle concentration.
Advice:
If you click Clear List, the sample information will be removed from the list.
window method list (List of methods). Click in this window on a measure to
toggle between their respective methods.
59
Note:
If you click Clear List, only the sample data in the list will be deleted. Ready
of methods . You can still view the measurement data on the Analyze tab.
60
Analyze the results

This section provides information on how to analyze the results after a measurement
has been run.
1. Select the Analyze tab in the top menu.

2. Select a measurement in the Record selector on the left.
3. Choose the work area Summary, Size, Zeta, Particle Con
centration, Titration, or Custom.
Different charts are displayed in each work area by default. For
For example, the default Size work area displays a size distribution graph by
intensity, correlogram, and statistics table.
Figure 5.20 Default Size Work Area
61
Advice:
You can click <1/> on a graph and then <2/> to select other graphs.
data options to include in that chart. For example, the correlogram includes the
option to include data Transient Y Without filter . This is described later in the help
from ZS XPLORER and in the User's Guide.
Advice:
To customize the work area layout, click in the upper right corner and select a layout from the
pop-up menu.
record picker
The record selector to the left of the Analyze window displays a table of results. In this table, the results
can be sorted by parameter values, edited, or
select for display in the work area.
Figure 5.21 Record Selector
To sort by a parameter value, click the corresponding column header in the record selector. To
change the displayed parameters, click
to open the parameter selection window:
62
Figure 5.22 Changing the parameters in the table
The data displayed in the record selector depends on the project you have selected. Click the explorer
arrow icon to display the Project Explorer.
Figure 5.23 Switch between projects
63
Click on the required project to display it in the Record Selector.
Note:
Every draft has its own table. You can choose in which project the result will appear
when setting up the measure.
64
CHAPTER 6 MAINTENANCE
About maintenance procedures 66
Cleaning of cells and accessories 66
Cleaning the covers 66
65
CHAPTER 6 MAINTENANCE
About maintenance procedures

Note:
Maintenance procedures and details of spare parts and consumable kits for any
accessory are listed in the accessories guide Zetasizer.
An operator can perform all of the maintenance procedures described in this section.
Cleaning of cells and accessories

Thoroughly clean the cells between each measurement, especially between different sample types.
Cross-contamination between samples can considerably affect the results.
See the Zetasizer Accessories Guide for information on cleaning and maintaining cells and
accessories related to Zetasizer equipment.
Cleaning the covers

CAUTION
System surfaces can be permanently damaged if samples are spilled
! or dispersants on them. If a spill occurs, disconnect the system from the source
before carefully cleaning.
Do the following to maintain the covers:
l Periodically and carefully clean the covers with a mild soap solution.
l Never use an excessive amount of liquid for cleaning and always avoid com-
electrical components (such as connectors) and cell windows.
l Never use a solvent-based solution for cleaning; can damage the super
ficie.
66
APPENDIX A SPECIFICATIONS
specs 68
chemical compatibility 71
67
CHAPTER A SPECIFICATIONS
specs
The following table lists the specifications of the Zetasizer. All specifications are correct at
time of publication, but may be subject to change.
Table A.1 Zetasizer Specifications
Size Measurement Specifications
Parameter Specification
0.3nm - 10ÿm* (Pro)

Range (maximum diameter)
0.3nm - 10ÿm* (15ÿmÿ) (Lab/Ultra)
12ÿL (Pro)
Minimum sample volume
3ÿL (Lab/Ultra)
Minimum Concentration - Non
Invasive Back Scatter (NIBS) (173 0.1 mg/mL protein 15 KDa (Pro/Ultra)
degrees)
Minimum Concentration: Forward Scatter 10 mg/mL 15 KDa protein (Lab/Pro/Ultra Blue) 5 mg/mL 15
(13 degrees) KDa protein (Lab/Pro/Ultra Red) 1 mg/mL 15 KDa protein
(Lab/Ultra Blue)
Minimum concentration: side scatter (90
degrees)
0.3 mg/mL protein 15 kDa (Lab/Ultra Red)
Minimum Concentration: Multi-Angle Dynamic

1 mg/mL 15 KDa protein (Ultra Blue and Red)
Light Scattering (MADLS)
Peak Concentration - Retro
non-invasive scatter (NIBS) (173 40% w/v † (bile acid) (Pro and Ultra)
degrees)
Maximum Concentration: Side Scatter (90

40% w/v †‡ (bile acid) (Lab and Ultra)
degrees)
90° and 13° (Lab)
measure angles 173° (water as sample dispersant) and 13° (Pro)
173° (water as sample dispersant), 90° and 13° (Ultra)
Dynamic Light Scattering (NIBS®), Multi-Angle Dynamic Light Scattering

measurement technique (MADLS®), Dynamic Light Scattering (13/90/173 degrees)
68
Zeta Potential Measurement Specifications
Size range suitable for

3.8nm minimum, 100µm maximum†
measure (diameter)
Zeta potential range No effective limitations
mobility range Zero minimum, no effective maximum
10 mg/mL protein 15 KDa (Pro)

Minimum sample concentration
1mg/mL protein 15KDa (Ultra)
Maximum sample concentration 40% w/v †
Minimum sample volume 20 µL (via Barrier Diffusion Technique: Proprietary)

Maximum sample conductivity 260 mS/cm
Conductivity Accuracy ±10%
Mixed mode measurement with light scattering analysis

measurement technique
phases (M3-PALS)
laser specifications
Model Specification
l Type: HeNe gas laser
l Max. Output power: 4mW
l Beam diameter: 0.63mm (1/e²)

Zetasizer Blue Label Systems
l Beam divergence: 1.5mrad
l Beam wavelength: 632.8nm
l Attenuation, transmission from 100% to 0.0003%
l Type: HeNe gas laser
l Max. Output power: 10mW
l Beam diameter: 0.63mm (1/e²)

Zetasizer Red Label Systems
l Beam divergence: 1.5mrad
l Beam wavelength: 632.8nm
l Attenuation, transmission from 100% to 0.0003%
69
General specifications
Detector Avalanche photodiode QE >50% at 633nm (Lab, Pro, Ultra)

condensation control Ease of purging using dry air
temperature control range 0°C to 120°C**
0°C to 120°C: glass and quartz cells

0°C to 70°C - low volume sizing cell
men
Compatible cell temperatures 0°C to 70°C - folded capillary cells, plastic cells
disposable
From 0 °C to 70 °C - immersion cell
From 0 °C to 100 °C - high concentration cell
Class 1 product laser, EN 60825-1:2014 and CDRH, LVD,

product compliance
EMC,RoHS
Dimensions 322mm, 565mm, 245mm (W, D, H)
Weight 19kg
Power requirements AC 100-240 V, 50-60 Hz, 4.0 A
Energy consumption Max. 220W
+10°C to +35°C (+50°F to +95°F) 10% to 90% of

Operating environmental conditions
RH (non-condensing)
computer interface USB2.0
Contact the help desk or go to the website of

Malvern Panalytical for the recommended specification of the
Recommended Computer Specification
computer. You can also check the
software update notification document provided on the software CD.
* Maximum mode range (diameter) from 0.6 nm to 10 ÿm, sample and sample preparation
dependent
** Temperature accuracy, 0.1°C to 25°C, 0.2°C to 0°C, 0.5°C to 90°C, 2.5°C to

120°C
† Depending on the sample
‡ With ZSU1002 ultra low volume sizing cell
ÿ Indicative data available for particle sizes greater than 10 ÿm when using
an extended size range analysis
70
MADLS® Multi-Angle Dynamic Light Scattering
chemical compatibility
Zetasizer components that may come into contact with the sample have been manufactured
from materials that are considered to give the greatest protection against degradation.
However, it is important to check that any sample or titrator used is chemically compatible
with the materials mentioned.
CAVEAT!
Malvern Panalytical advises that prior to inserting a sample you should check its
! chemical compatibility with the materials identified below. It is also recommended
to perform a test of the material with the sample before continuing with a use.
more permanent.
For details on cleaning and maintenance procedures, see Maintenance on page 65.
cell chamber
The only time the cell area can come into contact with the sample is
when a bucket or cell spill occurs. The list of materials below details all the components that
may come into contact in the event that this
happen.
Table A.2 Cell Chamber Materials
Component Materials
high impact polystyrene

set of the
cell bucket The exterior of the cell bowl and the top of the cell lid are
(lid, bucket and coated with a solvent resistant paint. This paint shows strength properties
Drainage channel) similar to polypropylene. The interior of the cell cuvette
and the drainage channel are not lined.
Drain pipe
Tygon, F-4040-A.
electrodes Beryllium/gold plated copper

Cell holder Aluminum (anodized)
71
Cells and cuvettes

See the descriptions in the Zetasizer Accessories Guide to see the materials used in each
of the available cells.
72
REGULATORY APPENDIX B
CE Declaration of Conformity 74
Canadian regulatory information (for Canada only) 74
VCCI Acceptance (Japan only) 74
FCC Notice (for USA only) 75
Disposal of electrical and electronic equipment 76
73
CHAPTER B REGULATORY
CE Declaration of Conformity
The CE label on this product complies with the relevant European directives. To get
For more information, see the Declaration of Conformity certificate for the product.
Canadian regulatory information (only for

Canada)
This digital apparatus does not exceed the Class A limits for radio noise emissions from digital apparatus
set out in the Radio Interference Regulations of the Department of
Canada Communications.
Please note that Canadian Department of Communications (DOC) regulations provide that changes or
modifications not expressly approved by Malvern Pana lytical could void your authority to operate this
equipment.
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numerique de la classe A is in accordance with the norme NMB-003 du Canada.
VCCI Acceptance (Japan only)

The "Voluntary Control Council for Interference" (VCCI) mark on this product signifies the
compliance with Japanese EMC regulations as specified by VCCI.
Translation:
This is a Class A product based on the "Voluntary Control Council for Inter ference" standard by the
Information Technology Team (VCCI). If this equipment is used in a
domestic environment, radio interference may occur, in which case the user may be required to take
corrective action.
74
FCC Notice (for USA only)

The "Federal Communications Commission" (FCC) mark on this product indicates that it is in
compliance with the FCC rules relating to radio frequency devices.
The product was tested and found to comply with the standard:
FCC CFR 47 Part 15: October 2011 (Class A digital device)
The device complies with part 15 of the FCC Rules. Operation is subject to the following two
conditions:
1. This device may not cause harmful interference, and

2. This device must accept any interference received, including interference that may
may cause undesired operation.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference
in which case the user will be required to correct the interference at his own expense.
Note:
Changes or modifications not expressly approved by Malvern Panalytical could void
the user's authority to operate the equipment.
75
Disposal of electrical and electronic equipment

When the need arises to dispose of the system, it must be done responsibly.
Follow these guidelines:
l Check local regulations on disposal of equipment; in Europe see below for the following
information.
l For more information, ask a local Malvern Panalytical representative.
l Decontaminate the instrument if hazardous materials have been used.
The following applies to the European Union and other countries in Europe with separate
collection systems.
This symbol on the product or on its packaging indicates that when

the last user wishes to dispose of this product, it should not be treated
as general waste. Instead, it should be delivered to the appropriate
facility for the recovery and recycling of electrical and electronic
equipment.
If this product is not disposed of with other household waste, the

volume of waste sent to incinerators or landfills will be reduced and
natural resources will be conserved.
For more detailed information on the recycling of this product, please

contact your local city office, waste disposal service or your Malvern
Panalytical representative.
76
MALVERN PANALYTICAL
Malvern Panalytical Ltd. Malvern Panalytical B.V.
Grovewood Road, Malvern, Lelyweg 1,
Worcestershire, WR14 1XZ, 7602 EAAlmelo,
United Kingdom The Netherlands
Phone +44 1684 892456 Phone +31 546 534 444

Fax. +44 1684 892789 Fax. +31 546 534 598
MAN0593-07-ES www.malvernpanalytical.com

En Assets MAN0593 07 ES Zetasizer Advance Guía Básica Tcm50 95452

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Chapter 1 Introduction one

About the Zetasizer Advance Series two

About this manual 3

Where to get help 6

Chapter 2 Health and Safety 9

General security issues 10

General maintenance warnings 17

Sample Handling Warnings 19

Chapter 3 Location Requirements twenty-one

Services of the Autotitrator MPT-3 27

Chapter 4 Hardware Overview 29

Chapter 5 Quick Start 41

Start the system 42

fill the cells 43

Loading of cells in the equipment 48

Analyze the results 61

About maintenance procedures 66

Cleaning of cells and accessories 66

Cleaning the covers 66

Canadian regulatory information (for Canada only) 74

VCCI Acceptance (Japan only) 74

FCC Notice (for USA only) 75

Disposal of electrical and electronic equipment 76

About the Zetasizer Advance Series two

About this manual 3

Where to get help 6

About the Zetasizer Advance Series

l Automatic titration measurements if an MPT-3 Autotitrator is connected.

Other key parameters such as conductivity, concentration of

Table 1.1 Characteristics of the Zetasizer Advance series equipment

Zetasizer Advance Series Kits

Forward scatter Zeta Y Y Y Y Y Y

Laser power (mW) 4 4 4 10 10 10

pH titration EITHER EITHER EITHER EITHER EITHER EITHER

"O" indicates an optional feature.

About this manual

Table 1.2 Equipment Information

Team part number

Product Documentation Structure

l Provides information about system location requirements.

l Provides a brief description of the measurement process. l

l User Guide: Provides detailed information on how to perform a measurement and

Software Option Selection

Where to get help

Telephone: +44 (0) 1684 891800 or email: helpdesk@malvernpanalytical.com.

If you are in the UK, call the following phone number:

+44 (0) 1684 891800 or email: cus

Phone: +1 800 279 7297 or email: support.us@malvernpanalytical.com.

CHAPTER 2 HEALTH AND SAFETY

General security issues 10

General maintenance warnings 17

Sample Handling Warnings 19

CHAPTER 2 HEALTH AND SAFETY

General security issues

Purge Warnings (MPT-3 Autotitrator)

CHAPTER 2 HEALTH AND SAFETY