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ANALYSIS REPORT ACCESSION #
FOR CLINICAL ASSESSMENT BY A MEDICAL SPECIALIST APR 15 2021
ONLY
IIIIIIIHIIIIIIII
OTHERS DO NOT TRY TO INFER A CUNICAL STATE FROM LAB DATA 17:00

STATUS: EMAIL ZPaid-NAG

NAME RAJAT JAYVEER [C846] 25Y-M GEN 16/04/2021 06:51 1 SEcnoN(s) V

REFDBY UID-7988 1280 3761 TAT 16/04/2021 17.00 DOMICILE SUB-
BOS

Parameter Assayed Result - Protocol Specific Lab Reference Interval -Comments

INFECTIOUS PANEL Section 1

1 Specimen OP/NP URT Swab in VTM
2 PCR SARS-CoV-2 Negative RT-PCR (E,RdRp,ORF1/N2 Genes)
Ref:
O SARS-CoV-2: Is the causative agent for COVID-19 (Corona Virus Disease-2019) in humans. A Beta Corona Virus, enveloped, non-segmerited, positive sense RNA virus belonging to the family Coronaviridae; order
Nidovi rales.

• Ideal Time to Test: During the Acute Phase of Infection Onlv

Researchers found that the probability of a false negative result decreases from 100% on Day 1 of being infected to 67% on Day 4. The false negative rate decreased to 20% on Day 8 i.e., 3
days after a person begins experiencing symptoms. They also found that on the day a person started experiencing actual symptoms of illness, the average false negative rate was 38%. In
addition, the false negative rate began to increase again from 21% on Day 9 to 66% on Day 21. - Annals of Int Med, May,2020; DOI: 10.7326/M20-1495
• Post-Recovery Re-Test Not Recommended Left over RNA fragments in resp. epithelial cells may continue to be amplified by PCR for some months causing a False Positive result.

SARS-CoV-2 Qualitative RT-PCR Test: Real Time Reverse Transcription Polymerase Chain Reaction protocol employed is based on * The ICMR has issued a Negative Recommendation against using Cycle
Taqman chemistry for detection of SARS-CoV-2 RNA in human NP/OP/BAL specimen from the Upper Respiratory Tract. Viral RNA is Threshold value for determining infectiousness & deciding patient
first extracted and converted into complementary DNA (cDNA) by the RT enzyme followed by thermal cycling. Positive amplification management protocols citing:
causes the dual labeled fluorescent probe to release fluorophores in an exponential manner which is captured on a real time basis. The
Cycle threshold (Ct) is the number of amplification cycles required for the fluorescent signal to cross the threshold. An internal positive • Although, theoretically the CT value is inversely proportional to the
control (IPC), validates each test through a full process control that checks all steps - from extraction to amplification thereby validating amount of RNA in the source URT swab of this QUALITATIVE
the test from quality of sample to intregrity of result. test; there is no reliable evidence of any direct correlation
between disease severity or infectiousness & CT value
1. A POSITIVE result indicates presence of SARS-CoV-2 RNA in the specimen tested • Studies have shown that guiding clinical decision based on CT
° Clinical correlation with patient history & other diagnostic information is necessary to determine infection ststus values of RT-PCR test has several limitations eg: the amount of
° Positive results do not rule out co-infection other bactaeria or viruses genetic material collected in URT swab unlike blood is quite
2. A NEGATIVE result DOES NOT exclude SARS-CoV-2 infection particularly if tested before onset of symptoms. Result must variable. CT will thus not reflect true viral load. Besides, observed
be combined with clinical observations, patient-history, & epidemiological information value will depend on multiple factors - Quality & Type of swab,
3. INCONCLUSIVE result may be encountered occasionally Type of VTM, Target gene used by different PCR methods, host
° Indicating that the test detected only one nucleic acid target common to SARS but not the specific targets to immune factors etc.
confirm SARS-CoV-2. A repeat test with a fresh sample will be required as soon as possible i.e. during the
acute phase

Approved by ICMR (ID-SENDPLP) & Health Dept Bihar

If you test POSITIVE for COVID-19, you undertake to strictly follow the guidelines issued by the State Govt, and HOME QUARANTINE yourself for 14 days. You are also required to regularly share ๐ status of your
health & that of your close contacts with the State Surveillance Team 0104
O COVID-19 «iTtl gl<l 3n5(5ld Mim CJJ'W ^4 14 dcfi 'HOME QUARANTINE1 I 3IM4I ^4
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** First Private PCR Lab in Eastern India - Est. 1996 **

 ANISABAD BORING RD DANAPUR GARDANIBAGH KANKARBAGH KHAGAUL PATALIPUTRA PATNA SAHEB PHULWARISHARI RAJABAZAR RAJENDRA
93344 85079 99552 97982 966112306 70700 98528 88098 F
99343 NGR
22597 30022 23333 95227 0 09990 43255 01784 98358 32736 37755 98526
ESTABUSHED BY:
Dr.Dilip Sen THE Dr Pratibha Srivastava Dr Priyanka Narain Dr Indroneel Sen Dr Abhiniti Srivastava Dr Sandeep Sen
PIONEER OF LABORATORY MBBS (#31818) MD (Path) MBBS (#33003) MD (Micro) MBBS (#45977) MD (R) MBBS (#18208), MD (๓ MBBS (#23776), FRSTM&H (Lon), FIAP-ID, FIC.Path
MEDICINE IN BIHAR Consultant & Chief of Consultant & Chief of Pathology Immuno-Hematology & TM MD, Diplomate of the American Board (Path,Hemat)
Histo-Cyto Division Microbiology Division Lab Medicine Patient-Care Director, Laboratory Medicine

o ASSAYEDWTTH ACCREDITED PROTOCOLS & SYSTEMS FROM ABBOTT, BD, BECKMAN-COULTER, BIO-RAD, OLYMPUS O CROSS-PROTOCOL REFERENCING NOT POSSIBLE * PLEASE CONTACT FOR ANY CLARIFICATION
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