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Amylase, Urine
TEST:
003293
CPT:
82150

Test Includes Amylase on random or timed urine collections

Special The test request form must state date and time collection started, date and time
Instructions collection finished, and urine volume.

Expected 1 - 3 days
Turnaround
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for
testing to when
Time the result is released to the ordering provider. In some cases, additional time should be
allowed for additional
confirmatory or additional reflex tests. Testing schedules may vary.

Related Urine Specimens

Information

Related Sample Report

Documents

SPECIMEN REQUIREMENTS

Specimen Urine (timed)

Volume 10 mL aliquot

Minimum 0.5 mL aliquot (Note: This volume does not allow for repeat testing.)
Volume

Container Plastic urine container without preservative

Collection Collect timed specimen. Instruct the patient to void at the beginning of the collection
period and discard the specimen. Collect all urine including the final specimen
 voided at the end of collection period.

Storage Room temperature

Instructions

Stability
Temperature Period
Requirements
Room temperature 14 days

Refrigerated 14 days

Frozen 3 days

Freeze/thaw cycles Stable x3

Causes for Presence of acid preservative (pH <3); improper labeling

Rejection

TEST DETAILS

Use Work-up for abdominal pain, epigastric tenderness, nausea, and vomiting. An enzyme
with molecular weight of 45 to 55 kilodaltons, urinary amylase is used in the
differential diagnosis of pancreatitis. It is very useful in diagnosis of pseudocyst of the
pancreas, where the urine amylase may remain elevated for weeks after the serum
amylase has returned to normal, after a bout of acute pancreatitis.

Methodology Enzymatic

Reference
Age Male (U/hr) Female (U/hr)
Interval
0 to 17 y Not established Not established

>17 y 0−18 0−17

Additional Macroamylasemia is characterized by high serum amylase but normal urine amylase.
Information The amylase:creatinine ratio remains useful for the diagnosis of macroamylasemia,
but its nonspecificity has otherwise left it with few other applications. In
macroamylasemia the clearance is very low.1
 Unlike serum amylase, urine amylase levels are normal with renal failure. While
serum amylase usually returns to normal within three to five days, without
complications urine amylase is increased longer than serum amylase in acute
pancreatitis. Two-hour collections are more practical and provide results sooner than
longer collections.

The major test for pancreatitis additional to serum and urine amylase is serum lipase.
It has good specificity and its laboratory analysis is greatly improved from the 1960s.
Also, some patients with pancreatitis have very high triglyceride levels.
Immunoreactive trypsin is not available stat and is not widely available at all.

Footnotes 1. Eckfeldt JH, Levitt MD. Diagnostic enzymes for pancreatic disease. Clin Lab Med.
1989 Dec; 9(4):731-743. PubMed 2480201

References Street HV, Close JR. Determination of amylase activity in biological fluids. Clin Chim
Acta. 1956 May-Jun; 1(3):256-268. PubMed 13343527

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