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Photoacoustic imaging will be performed by pulsing light through two optical fiber
bundles integrated with both sides of a rectangular, linear array transducer. The
laser excitation (Q-switched Nd:YAG) used 5 ns to 9 ns pulses at 10 Hz to 20 Hz
(6 Hz to 8 Hz frame rate). The ultrasound resolution will be controlled by changing
between three transducers: LZ-201 (center frequency = 16 MHz), LZ-250 (center
frequency = 21 MHz), and LZ-550 (center frequency = 40 MHz). Typical gains are
15 dB for photoacoustic signal and 10 dB for ultrasound. Operator will put on near-
infrared protective laser goggles during experiments. A layer of ultrasound gel will
apply to the transducer, which will adjusted to 1 cm from the tooth. The 532 nm to
680 nm laser will initialize, and the stepper motor will scanned 17 mm (0.076 mm
step size) to obtain a 3D PA-US image via maximum intensity projection.