EXPERIMENT –

AIM:- TO SEPARATE THE COLOURED COMPONENT’S

PRESENT IN THE MIXTURE OF RED AND BLUE INK BY
DOING PAPER CHROMATOGRAPHY AND FIND THEIR R.F.
RATIO.
MATERIALS REQUIRED:- GAS JAR, FILTER PAPER STRIP
(WHATMAN FILTER PAPER 1), JAR COVER, FINE
CAPILLARY TUBE, ALCOHOL, DISTILLED WATER,
MIXTURE OF RED AND BLUE INK.
PROCEDURE:-
1. TAKE A WHATMAN FILTER PAPER STRIP AND DRAW A
LINE WITH PENCIL ABOVE 4CM FROM ONE END.
DRAW ANOTHER LINE LENGTHWISE FROM THE
CENTER OF PAPER.
2. WITH CAPILLARY TUBE PUT A DROP OF INK MIXTURE
AT RHE POINT P. LET IT DRY IN AIR AND PUT
ANOTHER DROP OF INK ON THE SPOT AND DRY
AGAIN. SO THAT THE SPOT IS RICH IN MIXTURE.
3. SUSPEND THE FILTTER PAPER VERTICALLY IN JAR
CONTAINING THE SOLVENT (ELUENT) WITH THE HELP
OF GLASS ROD IN SUCH A WAY THAT THE PENCIL
LINE REMAINS ABOUT 2CM ABOVE FROM THE
SOLVENT LEVEL (50% ALCOHOL +DISTILLED WATER).
4. COVER THE JAR AND KEEP IT UNDISTURBED. NOTICE
THE RISING SOLVENT ALONG WITH THE MIXTURE OF
RED AND BLUE INKS. AFTER THE SOLVENT HAS RISEN
ABOUT 15CM YOU WILL NOTICE TWO DIFFERENT
SPOTS OF BLUE AND RED COLOUR ON THE FILTER
PAPER.
 5. TAKE OUT THE PAPER AND MARK THE DISTANCE
THAT THE SOLVENT HAS RISEN ON THE PAPER. THIS
IS CALLED SOLVENT FRONT.
6. DRY THE PAPER. PUT PENCIL MARKS IN CENTRE OF
THE BLUE AND RED SPOTS.
7. MEASURE THE DISTANCE OF 2 SPOTS FROM ORIGINAL
LINE AND DISTANCE OF SOLVENT FROM THE
ORIGINAL LINE.
8. CALCULATE THE Rf VALUE OF THE BLUE AND RED
INKS BY USING THE FORMULA :
Rf =DISTANCE TRAVELLED BY BLUE/RED INK FROM POINT

DISTANCE TRAVELLED BY SOLVENT FROM ORIGINAL

SOLUTION
S.NO SUBSTANCE DISTANCE DISTANCE Rf VALUE
TRAVELLED TRAVELLED
BY BY
DIFFERENT SOLVENT
COMPONENT
S
1. RED INK + A CM (RED X CM
BLUE INK INK ) A/X
2. RED INK + B CM ( BLUE X CM B/X
BLUE INK INK)

RESULT :-
1. RF VALUE OF RED INK =
2. RF VALUE OF BLUE INK =

PRECAUTIONS:-
1. KEEP THE STRIP ERECT. DO NOT LET IT BE CURLED.
2. DO NOT ALLOW THE SPOT TO DIP IN THE SOLVENT.
3. A SPOT SHOULD BE SMALL AND RICH IN MIXTURE.
4. KEEP THE JAR UNDISTURBED AND COVERED DURING
THE EXPERIMENT.