ETHODS OF PURIFICATION OF ORGANIC COMPOUNDS ‘Sublimation: Solid State —» Vapran giete (direst) * Cammpher, naphthalene, fn tra ume goxtiresion Gengeic acid. ek Ss vapnn sil opi Alayer of wet filter paper or ‘loth sublimate fest in separating sublimable compounds rom nowsblimatie [crystallisation ; THA beyed om Me diftenemee in and ie tmparcty i a Suibable Selvemt seep © preparation of the Selukion ye. - @® Filtration of pe Soluhim(uer) If a @ Gygtalligahon 7 @ dying of Cyshle cay on © Remeval of Calan wilh ackvaked — chancood chencoal absecky alk Caleuned Impani Distillation : 4 cool ‘ a Li, > (eps) Sin br otf voy high = +e te gee FG utifction of Lauids ~Distiation PIT eye eeeFractional Distillation: 3 6p diff isto - TCC Eee FE Se etyso @ cig=! eh w+ eae > Fyothonad clishllabion ob Gude oil AtS Cpodedemn) HK ticity LD kgendy om Leng of He. calumn engo-ke nunels sReeennerf) 9 Smp Troastey tuto ER gti, ma Bare gok gree Gant Sas) Gye 9 HEBE TU 1a eG (sean) pres BD) Gre te Suge Gore in Super tndustey Distillation Under Reduced Pressure/Vacuum © — hin 8? Ly key chimnpase ot ik — Tome we Gen tepancle Cite aak clatempogiim of Gepoundy by educthy preg: > BP Pu tue Pra C4 mm Hy) | ed” eb prow Pups F< He mm oy faces of acum Distiiaon BIC? LEME. obbatned ee _ eara A soltd_[ie 72 a Grsdble tr He iy meleaaban mys = hish Vepen pruduce ok $93 = meyt be Sleam velahile= fA sein pede hese Water inlet vit be Sleamm valahile we 4 Denpant ty — men val He Distilled liquid a-pleed) Guy Process of Seam istlation uy who 8 P, = WOH 1g MUAH See oor pomp ME Mino Gp Differential Extraction: a ov, ded hag SNH SALAbIK, Sn enti ond gee evs. Sein itl Swit anise £ era tate el Ear Differential Extraction Chromatography: phys; cal Method Chroma —+ Colour Gyaghg > serihing At Ue Used for Separation of Cdeuned Subptemey found Im plomts Tre Technique of Separating He Compments of & mithahe tn athich Separation tx achieved by He differential moveramt of indiviclual Components Hervnagh & Statimany phase undn He influence of @ rmobile phege ” cele Chrramakgraphy LL, pranaemege chvomatogvephy GHEE) © rasan iemaiogeaey > Papen Crvematogrep iy Taw Tops @ nepa ‘Adsorption Chromatography “oy Is based on the princi thatthe constituent re adsorbed an an adsorbent in | varying depres. The adsorbents used are general ei rai. When tmobile phase moves over the ted phase, ferent constuens of the mre at odsorbedat varios dstnces over the fed phase.aa = , Amixture is separated over a column of either silica gel or alumina, packed ye fates aera mcinaioeneehaemnscetnie an an ie, ese ree eae a cluant. The solvent is then evaporated to get the constituent Thin Layer Chromatography (tLe J ‘Assheet of alumina is taken (0.2:mm thick) over which a small ‘pot of the mixture is placed and it's kept ina suitable solvent. Adsorbent coated The solvent rises due to capillary action and the constituents also sree pla, rise with the solvent depending on their differential adsorption, and thereby, they are separated aa Ales a ple RuHve Mlyerpken ob Comparing Cm te Jar ‘Sample spot clu Basel hee ane Solvent Ue Uke CHebkI by RE" T. vepen chide Hemntled by Subse | 2 Ha chong FD Releakn| Pabardation FY = disdeme raved 6) Celia Ninkyde ee” aes +) Partition Chromatography Principle-Paper Chromatography ‘The separation of the components from the sample mixture scarred ies ‘uty the process of partition ofthe components betwean 2 phases SH a Both phases are i liquid form In this process, the immiscible slid surface coated with the liquid surface on the stationary phase is in the fen tmobile phase. The liquid surface is immoblized by a stationary phase ‘which results in making ts a stationary phase. The mobile phase moves | om from the stationary phase and components get separated. The — Jon separation depends on different partion coefficien Partition Chromatography Applications: aoe sees =a + To separate and identify amino acids ve ment + To separate and identity carbohydrates T— See Uve ten