Hung et ob of maltouwernove (1.0 mgm) ypon treatment with 4 HC) at 120 °C fr dierent parts of time (10,15, and Figure 20 rin) va Row chemtry npstem. The reaction was monwtored by MALDS TOFS mensoremant ‘of maltotriose with 4M HCI at 25 °C for 10 min im » flow: system, 15% off glucose and 30% of maltose were obtained, while 55% of maltotriose remained. The rate of byetrolysis increased as the reaction increased. After the ‘acid treatment at 120 °C for 10 min, 99% of mahouriose was hydrolyzed t give 65% glucose and 30% mahose. ‘Compared with Supplemental Figure S2, it seemed that the saccharide of higher size would slow down the bydroty- at 120 °C for 10 min in a flow system. The hydrolysis of ‘maltotose apparently decreased in lower concentrations of HCL Thus, the hydrolysis of higher oligosaccharides, ‘such as mahtotetraose, was best conducted with 4 M HCI at 120°C (Fig. 6). After 10 min of reaction time, a mixture of ‘maltotetraose (5%), maltotriose (15%), mahose (0%), and glucose (30%) was obtained according wo the MALDI- ‘TOF-MS analysis. Further degradation occurred after too- ‘ger hydrolysis time (15 and 20 min}; only glucose and maltose were observed a the sedated tons at m/: 202 and 365, respectively, ‘We then investigated the degradanon of a dracchande that contained two different monosacchande components. in the flow chemistry system. Common sacchanides (e.g. amon exchange chromatography with pulsed amperomet- ic detechon (HPAEC-PAD) 1s often used for direct sepa- Fanon and detecice of the sacchande components by ‘elution with 2 strong base (NaOH)">* In comparison, conventional reverse-phase high-pressure quid chroma. tography (HPLC) is more easily accessed to separate the proper derivanves of sugar components, such as sugar- NAIM compounds.” In addition, HPLC can be linked eth MS for the analysis of oligosacchandes with prot dervanizanion."”"™ For glycan compositional analysis, those monoxacchandes obtamed from glycan hydrolysis, ‘were recycled into the flow system to generate the sugar- NAIM derivatives, even at low sample loads. The pre- pared sugar-NAIM derivatives were concentrated by rotary c\aporanon under reduced pressure and analyzed iby LC-MS without further punficatson. Taking lactose as an example, the glucose and galactose components were obtamed by hydrotyses un a flow chemrs- Se seaian Aes RADA Sieting Oe COS MARE sal Ge tee re ete cae The residue ‘capillary cobume and identified by. [70-775 clepyd Pos 2) Oy eneeg Oe NAIM ce smophore, Gle-NAIM and Gal-NAIM occurring at the reten- ‘Hon times of 13.3 and 13.9 mun were easily detected using a UV detector at a wavelength of 330 nm. The NAIM deriva- ves exhibated higher hydrophobicity than their parentGamba & Hcdrctaes matonrsons (1.0 mgm.) Paci perahan Gengan MM) pate 120°C unas panos wabau yang barbed (10, 15, dan 20 man) datar seter tare stra Rew Sparta Senger pergpinren WAL OS TOF AAS, manowrona deegan 4M HC parte 26 °C satan 10 mere dolar sister sean 15% ghbona dan 20% maton Sper. edarghan 55% matt ona tetap Lap. Redon mereaghat dengan mereparrnys suths ‘eek Sete perakan aan ada tutes 120°C setama 10 ment. 99% maitomrons Ahern menanshan 5% gona dan 0% mama Dbaringhan dergar Gamber $2 Tambshan. car eatys akar da dengan winter kbsh weg shan meerpertar ts taps drone Gambar Tambahan $4 mer-banrgkan ener nero matowrons pace pertahuar dengan 4 stm? MHC (pecia 120 °C eter 19 mers tar sessem afar. eres (Manat Ona tarroaknya menu pada Konstan HO yang lebih rendah Oengan dersiian, hcrobss oagonakande yar feb ong seper® ranctetraota Sang bik Stak tar dengan 4M HC: pacta 120 °C (Gb. 6). Senatahs 10 ment Waktu reakss carpvran manctet asa (5%), mat ose (15%). mations (SO%), dan gukosa (30%) dpercten merunt satin ALDI TOF-MS. Oegratan eon iarp.t tera setetah wats Iadrotais yang tbh lama (15 dan 20 man. hays Gumons dan matona yang Caran sebaga on tera” puma mw? (Man, dan Gal) Maron Sah Sapat Sedaka Gorgan MS fn arnt bacat metas yarG sama Petcema top eamnag at cernaaran aren dengan deta: angeromnat endows HPAEC PAD) seerg Sgunahan ut pemaahan Manns dan perndetetsaan barge arta Sega eka dengan bass haat (NAOM) 33-36 Sebage perbandngan, Caran tamennoret bertaran ing tase terbabh ctvome Oy hy APLC) lebih ntah cakes tL meerasahian wen yang pat dan haroenen guia sepen veryawa la NARA 22.37 Seam HPLC dapat demurghan dengan MS, Re arate cngmatante dengan Geman seteternya 3738 Ura gikan anata komposst mena yar Spercieh dan tedrotees ghar cine Sarg he dita eaten airan wrt mengharitan turunan gute NAM, babtan paca teban savpe! yarg rendah Turunan gut NAR yang iat Ghapas sebekenrya Spehathan dengan Pepa mat 3 bimah Wetanan terete dan dar ates Gergee (CUS tance pemamar lab ary Mergent aicons sebage cortoh kamgonen giukosa dae gata Spercteh Senger Medecine Gaiam sister aban soe. Sete petabatar NADA terran GCMAM dan Gat NAM Garainm organ CAS Rees Gapat dorsahkan (Pate kotor hapher C18 dan dedentthaw dengan LOFTS, (Bupet Gbr SS) Oengan menana NAM cho maphore, Gl NARS can GaiMABE yang tends parte watts retort 13.3 dan 123 mart mach ddetets mergpewtan detei.o’ UV paca pares getombarg 330 ren Turunen NAIM merry ochoman rary Mbt W97 Garoeda rehanyE