Honey Analysis 09.04.2022 Class 12 9 Ruba Usman bites tans Scanned with CamScannerCertificate Acknowledgment Aim Requirements Theory Procedure Test for minerals TEST FOR CARBOHYDRATES Observation table Result Bibliography Content 2 3 4 4 5 6 6 7 8 9 9 Scanned with CamScannereee] aolill deallell dyn: all dull 9 INTERNATIONAL INDIAN SCHQDL 20. BOX 5665, AJMAN POL Bo: AJMAN, UAE |T: +971 67408333 | info@iisajman.org | wwrw.lisajman.org CHEMISTRY INVESTIGATORY PROJECT CLASS XII CBSE REGISTER NUMBER: 2764/7418 This is to certify that the Chemistry project titled Honey Analysis has been successfully completed by Ruba Usman of class XII in practical fulfilment of curriculum of CENTRAL BOARD OF SECONDARY EDUCATION (CBSE) leading to the award of annual examination of the year 2021-2022 Qa See” Teacher-in-charge Submitted for the Practi n held at International Indian School, Ajman. w oN cal Examination on 48/4 /2022 External Examiner: ye & Scanned with CamScannerAim qo analyze the available honey for the presence of different minerals and carbohydrates. Requirements Apparatus . Test tube Test tube stand Burner Water bath awe Chemicals . Fehling solution A 2. Fehling solution B 3, Ammonium chloride solution 4, Ammonium oxalate solution 5, Ammonium oxalate 6. Conc. nitric acid 7. Potassium sulphocyanide solution Scanned with CamScannerPersaturated Nd for subsist Bee honey is Composed of fructose, Blucose, and water, in varyi proportions. It also contains several enzymes and oils, The Sic flavour depend on the age of the honey and the source of the nectar. Its coloured honey is usually of higher Quality than dark-coloured honey. Other high-grade kinds of honey are made by bees from orange Blossom, Clover, and Alfalfa, Well-known Poorer grade honey is produced from buckwheat, : Sugar i pees to feed their larvae a 8ar solution manuf. factured by ence during winter. Honey has a fuel value of about 3307 cal/kg [1520 cal/ Ibs}. tt readily picks up moisture from the air and is consequently used as a din baking. Glucose crystallizes out of ature, leaving an uncrystallized layer of MARKETED is usually heated by a 50.01 F] to dissolve the Crystals and is in. The fructose in crystallized honey honey standing at room temper: dissolved fructose, Honey to be special process to about 66C ul sealed to prevent crystallizatior ferments readily at about 16C. Scanned with CamScannerProcedure Test for minerals- 4. Test for Potassium:- 2ml of honey is taken in a test tube a ean er nd picric acid solution i Yellow precipitate indicates the presence of K+. solution is added. 2. Test for Calcium:- 2ml of honey is taken in a test tube and NH4CI solution and NH40H solution are added to it. The solution is filtered and to the filtrate 2ml of ammonium oxalate solution is added. White ppt. or milkiness indicates the presence of Ca2+ ions. 3. Test for Magnesium:- 2 ml of honey is taken in a test tube and NHACI solution is added to it and then the excess Ammonium phosphate solution is added. The side of the test tube is scratched with a glass rod. White precipitate indicates the presence of Mg2+ ions. 4. Test for Magnesium: 2 ml of honey is taken in a test tube and NHACI solution is added to it and then an excess Ammonium phosphate solution is added. The side of the test tube is scratched with a glass rod. White precipitate indicates the presence of Mg2* ions. Scanned with CamScanneree, TEST FOR CARBOHYDRATES- 4. Fehling's test: 2ml of honey is taken in a test tube and 1ml each of Fehling's solution A and Fehling's solution B are added to it and boiled. Red precipitate indicates the presence of reducing sugars. 2. Tollen's test: 2-3 ml of an aqueous solution of honey is taken in a test tube. 2-3ml of Tollen's reagent is added. The test tube is kept in a boiling water bath for about ten minutes. A shining silver mirror indicates the presence of reducing carbohydrates. Scanned with CamScannerObservation table Substance taken: honey slno Tests Observations Inference 1 Test for Yellow ppt is Potassium is potassium- observed present Honey + picric acid solution 2 Test for White ppt or Calcium is calcium- milkiness is not absent Honey + NH4cl observed soln+ NH40H soln. Filtered + (NH4)2C204 3. Test for White ppt is not Magnesium is magnesium- observed absent Honey + NH4OH (till solution becomes alkaline) + (NH4)3P04 4 Test for irons- Blood red color | Iron is present Honey + conc is observed HNO3, heated and cooled potassium sulphocyanide 5 Fehling's test- Red ppt is Reducing sugar Honey + 1 ml observed is present wnt, Scanned with CamScannereach of Fehling’s solution A and Fehling’s solution B 6 Tollens test- Honey + 2-3ml tollens reagent , test tube in water bath for 10 min. Shining silver Reducing mirror is carbohydrate is observed present Result 4. Potassium is present. 2. Iron is present. 3, Calcium is absent. 4, Magnesium is absent. 5, Honey contains reducing sugar. Bibliography www,projects.icbse.com\chemistry www.projectsyapa. comchemistry Comprehensive Practical Chemistry Google Images Scanned with CamScanner