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    Detection Of Tumor Cells Contamination in Peripheral Blood

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    16 views6 pages

    Detection of Tumor Cells Contamination in Peripheral Blood

    Uploaded by

    kaka_huzefa

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 6
    5 1999; 14: 6238, Copyright ©The Korean Academy of Medial Sciences Detection of Tumor Cell Contamination in Peripheral Blood by RT-PCR in Gastrointestinal Cancer Patients We analyzed the peripheral biood of patients with gastrointestinl tract canoer at diferent stages to assess the presence of carcinoembryonic antigen (CEA) mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), which we Used as an indicator for micreretastatic malignant cals. A total of 35 gastric, 24 colorectal, 4 esophageal and 4 biliary tract cancer patients and rine normal heaithy subjects were stuxied. No CEA mRNA was detected in the nine normal healthy volunteers. CEA mRNA was detected in 100% (10/10) of metastatic, 38.3% (3/9) of early gastric cancer (EGC), and 18.8% (3/16) resectable gastric cancer patients, respectively. In colorectal cancer, 55.6% /9) of metastatic cancers were positive for CEA mRNA, and 26.7% (4/15) Duke stage BIC showed Positive, One patient with stage Il gastric cancer who was negative CEA mRNA, initaly and turned postive during follow-up, developed multiple bone metastasis ‘one month later. Another stage Il patient, who wes posive for CEA mANA, preoperatively revealed early relapse in two months. These resuits suggest that the identification of circulating turnor cells using RT-PCR for the detection of CEA mRNA is feasible and this analysis may be a promising tool for early

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