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    A Petri dish was inoculated with bacteria from a throat infection patient and incubated with four antibiotic discs. After incubation, clear zones appeared around some of the discs, indicating that the antibiotics in those discs prevented bacterial growth. The largest clear zone was around the ampicillin disc, suggesting that ampicillin would be the most effective antibiotic to treat the patient's throat infection.

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    A Petri dish was inoculated with bacteria from a throat infection patient and incubated with four antibiotic discs. After incubation, clear zones appeared around some of the discs, indicating that the antibiotics in those discs prevented bacterial growth. The largest clear zone was around the ampicillin disc, suggesting that ampicillin would be the most effective antibiotic to treat the patient's throat infection.

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    RP6 4

    Uploaded by

    김철수
    A Petri dish was inoculated with bacteria from a throat infection patient and incubated with four antibiotic discs. After incubation, clear zones appeared around some of the discs, indicating that the antibiotics in those discs prevented bacterial growth. The largest clear zone was around the ampicillin disc, suggesting that ampicillin would be the most effective antibiotic to treat the patient's throat infection.

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    of 1

    BYA3 p.

    1-16 19/11/04 10:45 am Page 6

    LEAVE
    6 MARGIN
    BLANK

    4 In a hospital laboratory, a sterile Petri dish of nutrient agar was inoculated with bacteria from
    a patient with a throat infection. Four discs, each of which had been soaked in a different
    antibiotic, were placed on top of the bacteria. The dish was incubated at 37 °C. Figure 1 shows
    the appearance of the dish after incubation.

    Disc containing Disc containing


    tetracycline penicillin

    Disc containing Disc containing


    ampicillin streptomycin

    Figure 1

    (a) Explain why there are clear zones around some of the discs containing antibiotic.

    .......................................................................................................................................................

    .......................................................................................................................................................

    .......................................................................................................................................................

    .......................................................................................................................................................
    (2 marks)

    (b) It was suggested that ampicillin might be the best antibiotic to treat the patient’s throat
    infection. Give the evidence from the laboratory test to support this suggestion.

    .......................................................................................................................................................

    .......................................................................................................................................................
    (1 mark)

    APW/0205/BYA3

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