GROWTH OF LIPID CONTENT IN MICROALGAE (Chlorella

vulgaris Beyerinick [Beijerinck], 1890) CULTURED USING BROILER

CHICKEN WASTE

By

IRMAYANTI
F1E1 17 026

ABSTRACT

The study aimed to find out the growth and lipid content of C. vulgaris cultured
using broiler chicken waste (LTAB). This study used a Complete RandomIzed
Design (RAL) consisting of 5 treatments of LTAB media additions namely P1 (0.2
ppm), P2 (2 ppm), P3 (20 ppm), P4 (200 ppm) and P5 (Walne) with 3 repeats.
Theinitial culture is 105 sel.mL-1 that are cultured for 7 days using a plastic bottle
(v = 600 mL) with a culture volume of 300 mL. Microalgae density is calculated
every 2 days using a neubaur haemocytometer under a 40x magnification
microscope with the help of a hand counter. Microalgae are cultured at a light
intensity of 35 μmoles.m-2.s-1,temperatures of28⁰C, pH 7-8 and salinity of 28-29
ppt. microalgae are harvested in the final stationary phase using Whatman
microfiber filter paper (Ɵ=25 mm), dried (1 hour, 100⁰C) and smoked (5 hours,
450⁰C) then weighed to calculate dry weight and dry weight free of ash. The lipid
content of microalgae was analyzed using the modified Bligh and Dyer methods.
Today, the concentration of chicken manure in the culture media and the interaction
between the two affects the growth of C. vulgaris (p=0.000). Cell density, specific
growth rate and biomass productivity of C. vulgaris are highest found in LTAB
media at P4 (200 ppm) (p=0.000) with a cell density value of 598.01×104 sel.mL-
1,
a specific growth rate of 0.4481 sel.day-1 and biomass productivity of 2.2521
g.L.day-1. The highest lipids are obtained in microalgae in LTAB P3 (20 ppm)
media by 4.2024%(p=0.001)whilein P1 (0.2 ppm), P2 (2 ppm) and P4 (200
ppm), content Lipids do not differ from 1-2%. This study suggests that lipid
production using broiler chicken liveris not recommended for biofuel feedstocks
due to their low lipid content but can be considered as a substitute feed material for
fish oil once its fatty acid profile is known.

Keywords: C. vulgaris,growth, lipids,cultures, organic

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