BT 301 Biophysics

Tutorial Sheet-V

1. If you shine linearly polarized light (obtained by superposing the left and right circularly
polarized lights) at a material, what would be the polarization of the light that emerges out if the
material is
(a) circularly dichroic but not circularly birefringent?
(b) circularly birefringent but not circularly dichroic?
(c) both circular dichroic and circularly birefringent?

2. Figure out the molecular formula(s) from the following data:

m/z = 106 (M; 100%) m/z = 107 (8.91%) m/z = 108 (0.05 %).

3. An unknown compound shows the molecular ion (M) peak at m/z = 170 with a relative
abundance of 50. The M + 1 peak has an intensity of 6.6, and the M + 2 peak has an intensity of
0.50. Predict the molecular formula of the compound?

4. An unknown hydrocarbon shows the peak at m/z = 84 (relative intensity of 31.3). The M + 1
peak has an intensity of 2.06, and the M + 2 peak has an intensity of 0.08. What is the molecular
formula of the unknown?

5. An unknown substance has a molecular ion peak at m/z = 107, with a relative intensity of 100.
The relative intensity of the M + 1 peak is 8.00, and the relative intensity of the M + 2 peak is
0.30. What is the molecular formula for this unknown substance?

6. Determine the molecular formula of the compound from the data given below.

7. Propose the molecular formula for the compound that gave this spectrum:
8. Propose the molecular formula for the compound that gave this spectrum:

9. Propose the molecular formula for the compound that gave the following spectra:
10. Consider that the mass spectrum shown below is obtained for a protein through the positive
ion mode ESI-MS. Calculate the mass of the protein from the data given