Mastercycler 5331 - 5334 Service Manual English

Mastercycler 5331 – 5334
Service Manual
English
Service Manual Mastercycler 5331 – 5334, English 5332 900.031-12
Safety precautions 1
PACT information sheets 2
Error lists / Troubleshooting 3
Disassembly / Assembly 4
Alignment / Adjustment 5
Diagrams 6
Spare parts list 7
Technical data 8
Maintenance 9
Check / Inspection 10
(blank) Descriptions 11
(blank) EDP | Printer 12

Service Manual Mastercycler 5331 – 5334, English 5332 900.031-12
Contents
1 Safety precautions
Read carefully before servicing the device!..................................................................... 1-1
2 PACT information sheets
3 Error lists / Troubleshooting

Error lists / Troubleshooting.............................................................................................. 3-1
Error message: "(Mid, left or right)-Block defective"...................................................... 3-11
4 Disassembly / Assembly
Mastercycler 5332............................................................................................................... 4-1
Preconditions for disassembling the Mastercycler 5332................................................ 4-2
Removing PCB Computer incl. display ............................................................................ 4-3
Disassembling PCB Display............................................................................................... 4-3
Removing the power supply module ................................................................................ 4-3
Removing PCB Power unit ................................................................................................ 4-3
Removing the thermoblock and the heated lid ............................................................... 4-4
Disassembling the heated lid .......................................................................................... 4-4
Mastercycler 5331 / 5333 / 5334........................................................................................ 4-5

Preconditions for disassembling the Mastercycler 5331 / 5333 / 5334 ......................... 4-6
Removing PCB Computer incl. display ............................................................................ 4-7
Disassembling PCB Display............................................................................................... 4-7
Removing the toroidal transformer .................................................................................. 4-7
Removing PCB Power unit ................................................................................................ 4-8
Removing the thermoblock together with the heated lid............................................... 4-8
Disassembling the heated lid .......................................................................................... 4-9
KS/1109 Contents 0-1

Contents
5 Alignment / Adjustment
Calling up service functions .............................................................................................. 5-1
Hidden functions................................................................................................................. 5-1
Calculating the Peltier current limitation (PW) ................................................................ 5-4
Storing the Peltier current limitation (PW)........................................................................ 5-5
Mastercycler 5331 – 5334................................................................................................... 5-6

Replacing the heated lid .................................................................................................. 5-6
Replacing the thermoblock ............................................................................................. 5-6
Checking the sensor functions ......................................................................................... 5-7

Procedure for determining the degree of aging of the Peltier module ......................... 5-8
Temperature validation system......................................................................................... 5-8
6 Diagrams
Page
General diagram of Mastercycler 5331 – 5334 ..................................................... 5331 900.020
General diagram of Mastercycler 5332................................................................. 5332 900.112
7 Spare parts list

Mastercycler 5332............................................................................................................... 7-1
Mastercycler 5331 – 5334................................................................................................... 7-4
Spare parts list ................................................................................................................... 7-7
Recommended spare parts ............................................................................................... 7-10
8 Technical data
Mastercycler® 5331 and 5333 ........................................................................................... 8-1
Mastercycler® 5332............................................................................................................ 8-2
Mastercycler® 5334............................................................................................................ 8-3
0-2 Contents KS/1109

Contents
9 Maintenance
Maintenance of PCR Systems........................................................................................... 9-2
Operational Qualification (OQ) including electrical test ................................................. 9-3
Extra Maintenance Service of PCR Systems ................................................................... 9-3
Description: Maintenance of PCR Systems ..................................................................... 9-4
Description: Extra Maintenance Services of PCR systems ............................................ 9-5
Electrical safety test........................................................................................................... 9-6
10 Check / Inspection
IQ – Installation Qualification
OQ – Operational Qualification PQ – Performance Qualification ................................. 10-1
IQ – Installation Qualification ........................................................................................... 10-2
OQ – Operational Qualification........................................................................................ 10-6
PQ – Performance Qualification ...................................................................................... 10-7
11 Descriptions (blank)
12 EDP / Printer (blank)
KS/ 1109 Contents 0-3

Contents
0-4 Contents KS/0603

Please read carefully before servicing the device!
• All servicing is performed at the technician’s own risk!

• It is necessary to participate in a technical training course by
Eppendorf before carrying out repair and maintenance work on
Eppendorf products!
• Disconnect power supply before opening the device!
• Please only use original accessories recommended by Eppendorf.
• Using spare parts and accessories which Eppendorf has not
recommended can reduce the precision, accuracy and life of the
device.
• Eppendorf does not honor any warranty or accept any responsibility
for damage resulting from the use of parts from other manu-
facturers or improper repair work.
• These service documents are for reference purposes only.
• This manual is not subject to the update service.
MSTM/MMWT/ 1301 Safety precautions 1-1

1-2 Safety precautions MSTM/MMWT/ 1301

MSTM/MMWT/ 1301 PACT information sheets 2-1

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PACT-System
Product News Application News Commercial Info Technical Info
New service spare parts for Mastercycler 5331 / 5332 / 5333
1.) The newest software state for old PCBs "Computer" can be reached via software updates, which consist
of a set of EPROMs and an operating manual in corresponding language versions.
Mastercycler gradient software update with operating manual in German/English 5331 863.035
Mastercycler gradient software update with operating manual for Eppendorf Scientific 5331 863.043
Mastercycler gradient software update with operating manual in French 5331 863.051
Mastercycler personal software update with operating manual in German/English 5332 863.004
Mastercycler personal software update with operating manual for Eppendorf Scientific 5332 863.020
Mastercycler personal software update with operating manual in French 5332 863.039
The software updates 5331 863.000 (gradient) and 5332 863.004 (personal) have been cancelled.
2.) Mastercycler gradient 5331 PCB "Computer" with software (without display) 5331 861.008
The old PCB "Computer" and EPROMs (without display) 5331 800.009 stay available for a limited time.
PCB "Computer" with software (without display) for the new Mastercycler 5333 5333 854.106
These two new PCB "Computer" have soldered program modules. So, software updates will be possible
only via download from the Eppendorf homepage. The PCBs are compatible.
3.) RAM module and corresponding socket with battery for the PCB "Computer" are available.
These spare parts can be used in case of memory problems.
Example: Program loss after switching off/on the Mastercycler.
For Mastercycler 5331/5332/5333 RAM module 0013 591.458

For Mastercycler 5331/5332/5333 Socket with battery 0013 604.592
4.) The following soldering fuses are available for the PCBs "Power unit".
5332 810.008 5331 013.007
F1 0.5A, slow 0013 606.072

F1 0.75 A, high speed 0013 609.039
F3 1.5 A, slow 0013 608.695
F4 F3/F4 2.0 A, slow 0013 608.709
F4 3.0 A, slow (heated lid, fast version) 0013 612.889
F2 F5/F6/F7 10 A, high speed 0013 608.300
F2 25 A, fast 0013 609.004
5.) For the frame on the heated lid, a set of Velcro tape is available.
For Mastercycler 5331 and 5333 Set of Velcro tape 5331 858.007
For Mastercycler 5332 Set of Velcro tape 5332 859.007
1999-07-23 / MMT / U.Krohss 53319T01.doc 1 (1)

PACT-System
Garantieregelung für Mastercycler 5331 / 5332 / 5333 / 5334
Da es immer wieder Anfragen zu Garantiezeiträumen bzw. unvollständige Angaben bei

Reklamationen gibt, hier eine Zusammenfassung.
Für alle oben genannten Geräte gilt ein Garantiezeitraum von 24 Monaten.
Auf Service-Ersatzteile und Austauschteile (AT) gilt eine Garantiezeit von 1 Jahr.
Sollte ein Thermoblock ausfallen, werden folgende Informationen auf der Service Info
Line (SIL) benötigt, um eine Garantieentscheidung treffen zu können.
1. Seriennummer des Gerätes, siehe Geräterückseite.

2. Seriennummer des Thermoblocks (Silberner Aufkleber am Thermoblock).
3. Temperaturwechsel.
Wie bekommt man diese Information?
OPTION – Taste gedrückt halten und Gerät einschalten, siehe Anzeige.
Nur mit diesen Angaben kann eine zügige Reklamationsbearbeitung erfolgen.
Wir hoffen, hiermit alle offenen Fragen geklärt zu haben.
Warranty procedure for Mastercycler 5331 / 5332 / 5333 / 5334
Due to the fact that we continue receiving inquiries regarding warranty periods and/or
incomplete information in connection with claims, we summarize the procedure as follows:
All the above-mentioned devices are covered by a warranty period of 24 months.
Service spare parts and pool parts are subject to warranty for a period of one year.
In the event of a thermoblock failure, please enter the following items of information in the
Service Info Line (SIL), so that we will than be able to make a warranty decision:
1. Serial number of the device - please see the rear panel on the device.
2. Serial number of the thermoblock (silver-colored sticker on the thermoblock).
3. Temperature change. Where do you obtain these items of information ?
Keep the OPTION key depressed while switching ON the device - watch the display.
Only if we have this information are we able to deal with your claim promptly.
We hope that we have herewith resolved all pending questions.
2001-08-03 / / 53311T02.doc 1 (1)

PACT-System
New Software V 2.30 for Mastercycler 5331, 5333 and 5334.
Important note:
Save your programs on memory card before updating the software.
This new software is valid for all mastercyclers with flashloader.

Please check the type of Computer PCB in your mastercycler prior to the update:
Switch on mastercycler and note the software version displayed.
For instance, the 09 in V2.xx 31 – 09 indicates a Computer PCB with flash loader, which
can be updated via the interface.
Use data cable 5331 867.006 for this purpose.
This software can be downloaded from www.eppendorf-support.com.
The software update disk 5331 835.007 is no longer available.
Computer PCBs without flashloader can only be updated by replacing EPROMs.
Current software update as EPROM versions:

V 2.12 for Mastercycler 5331 for PCB Computer without flashloader 5331 865.003
V 2.22 for Mastercycler 5332 for PCB Computer without flashloader 5332 866.003
New 384-type sensor for the validation/calibration set = (VCS) (5331 222.005).
The Mastercycler 5334 and the 384-well thermoblock use a new temperature sensor.
This sensor is calibrated and works with the existing VCS. To assure accuracy, this
sensor must be adjusted to the VCS. This adjustment is carried out at 60°C. The offset
shift is described on page 19 of the Testo manual provided with the VCS.
Temperature sensor for 384-well thermoblock 5331 233.007 (new)

Includes the 384-type sensor, silicone mat, chip card 384 and instruction manual for 384-
well block.
Silicone mat 5331 234.003 (new)
Chip card 384 5331 235.000 (new)
Power unit PCB for 5331, 5333 and 5334 5331 866.000 (new)
Power unit PCB for 5332 5332 861.001 (new)
These PCBs have been modified to eliminate the „Power Error“.
Power unit PCB for 100, 115, 230 Volt 5331 013.007 (no longer available)
Power unit PCB for 100, 115, 230 Volt 5332 810.008 (no longer available)
2002-01-29 / / PACT53312T01.doc 2 (2)

PACT-System
Neues Temperatur-Validierungssystem
Es gibt ein neues Temperatur-Validierungssystem, mit dem die Mastercycler und Mastercycler ep
validiert bzw. justiert/kalibriert werden können.
Die Bestellnummer für dieses Temperatur-Validierungssystem lautet 0055 000.298.
Weitere Informationen erhalten Sie auf der eppendorf-support.com, in der Bedienungsanleitung
Temperatur-Validierungssystem.
Um einen Cycler mit einem 384er Thermoblock validieren bzw. justieren/kalibrieren zu können,
benötigen Sie zusätzlich den Sensor 384. Die Bestellnummer für den Sensor 384 lautet 5331 233.007.
Weitere Bestellnummern finden Sie im Kap. 7 des entsprechenden Servicemanuals. Sollten Sie ein
Validierungssystem mit der Bestellnummer 5331 222.005 haben, tauscht Eppendorf dieses System
kostenlos gegen ein Validierungssystem 0055 000.298 aus.
Der Austausch erfolgt nach Absprache über das SIL-Verfahren.
Für das Validierungssystem mit der Bestellnummer 5341 223.000, das als Zwischenlösung nur für die
Mastercycler ep eingesetzt wurde, erhalten Sie von uns kostenlos ein entsprechendes "Upgrade kit",
mit dem Sie ihr Validierungssystem auf das System mit der Bestellnummer 0055 000.298 aufrüsten
können.
New temperature validation system

There is a new temperature validation system with which the Mastercycler and Mastercycler ep can be
validated or adjusted/calibrated.
The order number for this temperature validation system is 0055 000.298.
You can find further information at eppendorf-support.com, in the Operating instructions for the
temperature validation system.
In order to validate or adjust/calibrate a cycler with a 384-well thermoblock, you also require the Sensor
384. The order number for the Sensor 384 is 5331 233.007.
You can find additional order numbers in Ch. 7 of the relevant service manual. If you have a validation
system with the order number 5331 222.005, Eppendorf will replace this system at no charge with a
validation system 0055 000.298.
The exchange is carried out after agreement with the SIL procedure.
For the validation system with the order number 5341 223.000, which was used as an interim solution
only for the Mastercycler ep, you will receive an appropriate "upgrade kit" from us free of charge, with
which you can update your validation system into the system with the order number 0055 000.298.
2005-05-26 / MST / UK Mastercycler_ep5T02 1 (1)

2-2 PACT information sheets MSTM/MMWT/ 1301

Troubleshooting
Programming errors or errors regarding the handling of the personal card can be eliminated after the
cause of the error has been established.
Technical errors can be caused by interference (e.g. power failure or power fluctuations). In some cases,
it is possible to eliminate the error by switching off the device for a short period. Wait ten seconds before
switching on the device again.
Error Cause Solution
BLOCK ChkSumErr Data error. Store calibration data and

programs (if necessary) as they
are lost when initializing occurs.
This can be done using
Function 5 (OPT+INS); see
Sec 5.
If this is unsuccessful, the sub-
assembly "Thermoblock" must
be replaced.
BLOCK DEFECTIVE Defective electronics, Note:

thermoblock or fuse. See Troubleshooting for
The time may have been Mastercycler 5332 and
exceeded. Mastercycler 5331 / 5333 /
5334.
Heated lid is on the
thermoblock.
The heated lid is open and there
is a draft.
MSTM/MMWT/1301 Error lists/Troubleshooting 3-1

BLOCK TOO HOT! Defective regulation or defective Switch off the device and switch
electronics. it on again.
This error occurs when the If the error recurs after the
thermoblock temperature device has been switched on
exceeds 110 °C. again, check the current to the
Peltier element (see Sec. 5,
"Calculating the Peltier current
limitation". If the current is
higher (approx. 8 amps) when
the service function has not
started, PCB Power Unit is
defective.
Card changed Card removed immediately Insert card correctly and repeat
before being inscribed. procedure.
Card content not o.k. Content of card is defective. Card must be formatted.
Automatic procedure when
program is saved.
Card not in slot Card removed during saving Insert card correctly and repeat
process. procedure.
EEProm ERR! EEPROM not found. Defective sub-assembly

"Thermoblock" or defective
connecting cable.
Enter Tube Not all entries have been made Enter tube type and sample
for a program which has been volume.
started.
3-2 Error lists/Troubleshooting MSTM/MMWT/1301

Err GOTO (1) Branching to a program line Check GOTO command and
number is impossible because modify or delete if necessary.
this program line number is in a Program line number for
program area which has already additional GOTO command
been selected for repeating must not be in a section which is
using another GOTO command. already addressed by a GOTO
command.
Err GOTO (2) More than three GOTO Three GOTO commands can be
commands used for one used in one program range
program range. when the areas which have
been addressed overlay each
other completely, i.e. the
program area of the second
command is greater than that of
the first command. The third
GOTO command must cover an
even greater area than that of
the second command.
Err Invalid Invalid command. Enter command completely or

Parameter has not been delete command.
entered.
Err Link (1) LINK command cannot be Only programs from the internal
... executed. memory can be called up by the
Line .. Program not found. LINK command. Programs on a
Program name and program personal card and the program
line of defective LINK command in the processing level ("Edit")
appear in the display. cannot be called up by the LINK
command.

Err Link (2) Too many programs are A maximum of five programs,
... connected by LINK commands. i.e. four LINK commands, can
Line .. Program name and program be processed in succession.
line of defective LINK command
appear in the display.
Err No Program No executable program Load program from memory or

available in memory. personal card, or define a
program in "Edit" menu and
restart.
FAN ERROR The small fan in the transformer Note: Error message for
area is defective. Mastercycler 5331 / 5333 /
5334 only.
Using the service function FAN,
check that the fan is functioning
properly; see Sec. 5.
FAT Full Number of programs which can Delete programs which are not
be administrated has been required.
exceeded.
Internal memory: 99 programs
personal card: 10 programs
Internal EE-Err Data error. Device must be reinitialized; see

"Special functions", Sec. 5.
LEFT BLOCK DEFEKTIV Defective electronics or Note:

defective thermoblock. See Troubleshooting for
Mastercycler 5332 and
Mastercycler 5331 / 5333 /
5334.

LID TOO HOT Defective electronics Using the service function LID,
This error occurs when the check the heated lid;
temperature of the heated lid is see Sec. 5.
> 120 °C. Set a temperature of 50 °C and
check whether this temperature
is attained and then maintained.
Information appears in the
display as to whether the device
is heating or not.
0 % = no, 100 % = yes.
If the temperature of the heated
lid rises even though the
regulator is at 0 %, PCB Power
Unit is defective.
Link not found Program branching is not Check the LINK command.
possible because the program Enter the missing program.
which was supposed to be
called up has been deleted.
Link Error Program branching is not Check the LINK command.

possible because the program Enter the missing program.
which was supposed to be
called up has been deleted.
MCard Error The memory of the personal Evaluate further messages.

card is defective.
Memory corrupted The internal memory is Load the programs.

defective or the battery is
defective or needs recharging.
The program memory has been
deleted.

Memory Error The internal memory for Evaluate further messages.

programs is defective.
Memory full! The program memory is full. Before storing, delete programs
which are not required.
MemoryCard Card is defective. Use a new card.

Defective!
MemoryCard HdChksumErr Error in check sum. Card must be formatted.

program is saved.
MemoryCard ManIDErr Incorrect identification. Card must be formatted.

program is saved.
MemoryCard Incorrect memory address. Card must be formatted.

MemOverErr! Automatic procedure when
program is saved.
MemoryCard Error in memory range. Card must be formatted.

MemRangeErr Automatic procedure when
program is saved.
MemoryCard Incorrect memory size. Card must be formatted.

MemSizeErr Automatic procedure when
program is saved.

MemoryCard MemUnderErr! Incorrect memory address. Card must be formatted.

program is saved.
MemoryCard No card has been inserted. Insert card.

Missing!
MemoryCard Card is invalid. Insert card correctly (check that

Not Valid card is facing in the correct
direction).
Card should be formatted

before saving begins.
MemoryCard Card cannot be read. Insert card correctly (check that

Read Err! card is facing in the correct
direction).
MemoryCard Card is marked as write- Card must be formatted.

Read-Only! protected. Automatic procedure when
program is saved.
MemoryCard Card has incorrect logical Card must be formatted.

StartIDErr identification. Automatic procedure when
program is saved.
MemoryCard Writing error on card. Insert card correctly (check that

Write Err! card is facing in correct
direction).
Do not touch/move card during
saving.

MID BLOCK Defective electronics or Note:

DEFEKTIV defective thermoblock. See Troubleshooting for
5334.
Name Invalid Program name contains an Enter program name again.

invalid character.
POWER ERR The external voltage supply is Check the mains voltage supply.
too low.
Check the transformer, fuses,
One or more internal voltage
rectifier and PCB Power Unit.
supplies is missing.
Printer: OFF LINE Printer is not ready to receive. Select the "ON LINE" printer
setting.
Printer: no response No printer is available or the Select "OFF" in the menu

printer is not switched on. OPTION/Printer/Print Protocol.
Switch on the printer.
Program is running! A program is being processed. Lid temperature and time

cannot be modified at present.
Program too large! Program is too large. Max. 40 program lines possible.

Restarting Program! A power failure occurs or the The program continues

device is switched off briefly automatically.
when a program is running.
RIGHT BLOCK DEFEKTIV Defective electronics or Note:

defective thermoblock. See Troubleshooting for
5334.
Slowing Down! The heating rate or cooling rate Using the service program,
has slowed down. check that the Peltier element(s)
Protection function. is/are working; see Sec. 5.
Carry out the aging test; see
Sec. 5.
During which program step
does the error occur?
Steps free Display of free memory spaces Delete programs which are not
is < 100 steps. required or store them on the
1 step = 1 program line. personal card.
TSENSORxxERROR! Defective temperature sensor. It is essential to specify the

TSensor xx number to
determine the correct unit
(thermoblock or heated lid). See
Sec. 5, Checking the sensors.
Value out of range! Value is not within the permitted The permitted value is entered
value range. automatically.

Warn Ramp Max The ramp is limited to 3 °C/s. Check entries for ramp and
... ramp increment, and change
Line... entries if necessary.
Warn Ramp Min The ramp is limited to 0.3 °C/s. Check entries for ramp and
... ramp increment, and change
Line ... entries if necessary.
Warn Temp Max Temperature limits of 99 °C Check temperature commands

exceeded. If program is started, and reduce value of
temperature is limited to 99 °C. temperature increment so that
only values below 99 °C can be
reached.
Warn Temp Min Temperature limit of 4 °C not Check temperature commands

reached. If program is started, and modify values of
temperature is limited to 4 °C. temperature increment so that
only values above 4 °C can be
reached.
Warn Time Max Cycle time of 9:59:59 exceeded. Check temperature commands
If program is started, cycle time and modify values of time
is limited to 9:59:59. increment so that only values
below 9:59:59 can be reached.
Warn Time Min Cycle time of 0:00:01 not Check temperature commands
reached. and modify values of time
If program is started, cycle time increment so that only values
is limited to 0:00:01. above 0:00:01 can be reached.

Error message: "(Mid, left or right)-Block defective"
This error message may be caused by the following:

1. Ventilation slits are blocked. Not enough space to allow air to flow under the device for cooling
purposes. Objects under the device.
2. Is the large fan running perfectly?
3. What was the room temperature and the humidity when the error occurred?
4. At what time did the error occur? Print out programm.
5. Thermoblock or PCB Power Unit defective.
6. What is the value for PW? Please print out parameter list. Goto: Serv. – Div. – Inits.
☞ If the thermoblock is defective, the temperature changes are required.
Display of temperature changes: Switch off device, hold down OPTION key while switching
on the device.
No warranty claims can be processed without this information!
For item 2
– Call up service function. (Page 5-1, service manual).
– Call up DIV – Fans – Testing Fans.
– PeltFan (This is the large fan).
☞ Switching between OFF and ON is possible via the Sel key!
– Check the crimp contacts by wobbling the fan connections.
For item 4
– Printing out the program.
– Goto: Main Menu-Options-Printer
– With Printout Editor, the program can be printed.
– With Print Protocol, the individual steps of the current program can be printed.
Each temperature change is controlled via a time frame. The time frame (Tim) can be observed in the
service function Block-Outputs.
Procedure:
– Start program.
– When the program is running, call up the service functions.
– Block-Outputs.
– With Mastercyclers 5331 / 5333 / 5334, select the corresponding block segment (0, 1 or 2).

Display: Output
I= D = –2.0°s
BStm = 51.0 P = –184 –184
Out =–1097 MO =184/184
Tim = 53.2°
DTmp = –2.2
Explanation:
I= Integration share of regulation
BStm = Nominal temperature
Out = Calculated output of regulation
Tim = This time frame checks whether the thermoblock is able to reach a temperature change in a
given time. If the thermoblock is too slow or the temperature cannot be held for a given time, the
message "BLOCK DEFECTIVE" is displayed.
DTmp = Temperature difference between actual temperature and nominal temperature
D= Temperature change per second.
P= Actual PW.
MO = max. possible PW.
53.2 °C Actual temperature
Press the Exit key twice to exit the function Block Outputs. Call up function Sensor.
Observe sensor C: (Heat sink of thermoblock).

When cooling the thermoblock and at a room temperature of 25 °C, this sensor has a temperature of
approx. 42 °C. If the temperature greatly exceeds 50 °C (e.g. because the ambient temperature is too
high or because of insufficient air circulation in the Mastercycler), this may cause the message
"Thermoblock defective".
For item 5
The thermoblock of Mastercycler 5332 consists of one block segment.
The thermoblock of Mastercycler 5331 / 5333 / 5334 consists of three block segments.
Viewed from the rear to the connection side of the thermoblock:
Segment 0 is the left block segment,
segment 1 is the middle block segment and
Segment 2 is the right block segment.
Call up service functions.

Goto: DIV-PELT-Calculation of PW
This function allows switching of the current for the block segments.
Switch off the AutoRestart function before calling up this function!

Goto: Main-Menu-Options-Generals-Etc.-AutoRestart.
Switch OFF function using the SEL key.
With this function switched ON, a program which has not been terminated continues to run after the
device has been switched on. This results in the thermoblocks being under current.

By entering –180 (cooling) confirmed with the Enter key, the corresponding block segment can be called.
With the block segment and the controlling from PCB Power Unit being OK, a current of approx. – 5 A at
– 180 and 5 A at 180 can be measured. The temperature for the block segment is changing.
If this is not the case, there are two error possibilities:

The thermoblock itself or the output for this block segment of PCB Power Unit is defective.
This output can be checked directly.
– Remove upper part of housing.
– Disconnect plug connection between PCB and thermoblock.
Mastercycler 5332 has a connector X7.
Mastercycler 5331 / 5333 / 5334 each have a red and blue connection per block segment.
For Mastercycler 5332, the multimeter can be connected to the output of the PCB via adapter cable
5332 625.006.
With the other Mastercyclers, the multimeter is connected directly to the red and blue wire coming
from PCB Power Unit.
The current is limited to max. ± 5 A by pulse-width modulation (PW), thus preventing a short circuit.
By controlling with – 180 or 180 it is now possible to check whether the corresponding output of the
PCB is able to deliver 5 A.
– If this is OK, the thermoblock error message is caused not by PCB Power Unit, but by the
thermoblock itself.
The thermoblock or the Peltier elements can only be checked with an AC Ohm meter. If such a high-
grade measuring instrument is available, an internal resistance of approx. 1.2 Ohm (5331 – 5334) or
approx. 2.1 Ohm (5332) can be measured for an intact Peltier element.


Mastercycler 5332
Disassembly sequence:
Preconditions for disassembling the Mastercycler 5332
Removal of thermoblock with heated lid *
Removal of fan Disassembly of heated lid * Removal of PCB Computer
Removal of power supply module
Removal of PCB Power unit
* After the thermoblock or heated lid has been exchanged, parameters (see supplementary sheet) must
be entered; see Sec. 5.
* For complaint processing, please provide the serial number of the faulty thermoblock and the number of
temperature changes.
Assemble in reverse order.
MSTM/MMWT/ 1301 Disassembly/Assembly 4-1

Mastercycler 5332
Base of device
4 screws for
power supply module
8 slots
Foot- Foot
2 screws for
upper housing
2 screws for
mounting plate for
PCB Power unit
4 screws for
thermoblock
+ heated lid
Fan
2 screws for
upper housing
Foot Foot
Fig. 1 Slots (front)
Preconditions for disassembling the Mastercycler 5332
Utilities: Phillips screwdriver, size 1
– Unplug the device.
– Take off the frame of the heated lid.

– Place the device on its side.
– Remove the four Phillips screws (for the upper housing); see Fig. 1.
– Turn the device around, carefully lift off the upper housing and pull off the two ribbon cables.
4-2 Disassembly/Assembly MSTM/MMWT/ 1301

Mastercycler 5332
Removing PCB Computer, incl. display
– The PCB computer is fastened with four Phillips screws.
– Remove the ribbon cable for the keypad.
Disassembling PCB Display
– The PCB display is fastened with four Phillips screws.
– PCB is connected.
Removing the power supply module
Place the device on its side and remove the four rear Phillips screws (for the power supply sub-
assembly); see Fig. 1.
– Pull off the ground connection.
– Pull off the 2-pin plug.
– Pull off the two individual plugs and the 6-pin plug from PCB Power unit.
Removing PCB Power unit
Precondition: Removing the power supply module.
– Disengage the two flex foils of the heated lid and remove.
– Pull off the ribbon cable on the thermoblock.

– Remove the two Phillips screws (for the mounting plate/power unit);
see Fig. 1.
– Carefully lift out PCB Power unit and place it next to the device.
– Pull off the two plugs for the fan.

Mastercycler 5332
Removing the thermoblock and the heated lid
– Remove the four Phillips screws (for the module "Thermoblock/heated lid" ); see Fig. 1
– Pull off the 2-pin plug.
– It is not necessary to remove the fan cable.
– Lift out the entire unit.
☞ Important!
After the thermoblock has been exchanged, parameters (see supplementary sheet) must be
entered.
Disassembling the heated lid

Phillips screwdriver, size 1
– Disengage the two flex foils of the heated lid and remove.
– Loosen the fan.

– Pull the flex foil shield from the clamp for the heat sink and the metal plate for the fan.
– Remove the two cover caps from the heated lid and press out the bar using a Phillips screwdriver.
– Remove the heated lid.
☞ Important:
After the heated lid has been exchanged, parameters (see supplementary sheet) must be
entered.

Mastercycler 5331 / 5333 | 5334
Disassebly sequence:
Preconditions for disassembling the Mastercycler 5331 / 5333 | 5334
Removal of thermoblock with heated lid *
Removal of fan Disassembly of heated lid * Removal of PCB Computer
Removal of power supply module
Removal of PCB Power unit
* After the thermoblock or heated lid has been exchanged, parameters (see supplementary sheet) must
be entered; see Sec. 5.
* For complaint processing, please provide the serial number of the faulty thermoblock and the number of
temperature changes.
Assemble in reverse order.

Mastercycler 5331 / 5333 | 5334
Base of device
Power supply module
2 screws for
upper housing
Foot Foot
Long hole Small fan
Large fan
2 screws for
upper housing
Foot Foot
Fig. 2
Preconditions for disassembling the Mastercycler 5331 / 5333 | 5334
– Unplug the device.

– Take off the frame of the heated lid.

– Remove the four Phillips screws (for the upper housing); see Fig 2.
– Turn around the device, carefully lift off the upper housing and pull off the two ribbon cables.
– Take off the two rubber air guides.

Mastercycler 5331 / 5333 | 5334
Removing PCB Computer, incl. display
– The PCB Computer is fastened with four Phillips screws.
– Pull off the ribbon cable for the keypad.
Disassembling PCB Display
– The PCB Computer is fastened with four Phillips screws.
– The PCB is connected.
Removing the toroidal transformer
Utilities: Allen wrench, 3 mm

– Remove the toroidal transformer together with the mounting plate. Remove the three screws for the
power supply module.
– Place the device in an upright position.

– Pull off the two connections to the bridge rectifier.
– Pull off the two connections to PCB Power unit.

– Disengage the 6-pole plug on PCB Power unit and remove.
– Lift the transformer out of the device.

Mastercycler 5331 / 5333 | 5334
Removing PCB Power unit
Utilities: Screwdriver, size 2
Precondition: Removal of the power supply module
– Remove the three slotted screws from the heat sink/PCB.

– Loosen the ground connection of the heated lid (middle screw).
– Disengage and pull off the flex foil of the heated lid.
– Pull off the six connecting pins.
– Pull off the flex foil.
– Remove the mains input module and pull off the three connections (black - G.G. - white).
– Separate the ground connection from PCB Power unit.
– Pull off both plugs for the fan.
– Reassign the connections of the bridge rectifier.
Removing the thermoblock together with the heated lid
– Remove the two Phillips screws (at the front of the heat sink).
– Loosen the two outer slotted screws from the heat sink/PCB.
– Unscrew the middle screw in order to loosen the ground connection from the heated lid.
– Pull off the six contact pins and the ribbon cable on PCB MUX.
– Pull off the flex foil leading from the heated lid to PCB Power unit.
– Lift out the entire unit.
☞ Important!
After the thermoblock has been exchanged, parameters (see supplementary sheet) must be
entered.

Mastercycler 5331 / 5333 | 5334
Disassembling the heated lid

Phillips screwdriver, size 1
– Remove the two Phillips screws and press out the bar using a Phillips screwdriver.
– Remove the heated lid.
☞ Important!
After the heated lid has been exchanged, parameters (see supplementary sheet) must be
entered; see Sec. 5.


Calling up service functions
– Switch on the device.

After a short period, the Main menu is loaded.
– Call up OPTIONS .... and confirm by pressing Enter.
– Call up GENERALS .... and confirm by pressing Enter.
– Enter 5 (6 from version 2.0 onwards) and confirm by pressing Enter.
– Enter 9, 8, 7, 6; the Service mode is switched on.
Hidden functions
Key Function
OPT Response: Two beeps.
Purpose: To display the temperature-change counter without entering into the service
menu.
DEL+ Response: Two beeps.

Following initializing: Device restart.
Purpose: Obligatory initializing of the non-volatile program memory.

All programs are deleted. However, the calibration data is not deleted!
This should be carried out when unusual messages / error messages (Internal
Err xxx) appear in the display.
DEL+ Response: Two beeps.

"Errors Off" appears in the display when the error messages are deactivated.
Purpose: To switch on/off all error messages. This allows defective devices to be
operated for test purposes (Temp sensors).
OPT+INS Response: Four beeps.

Hold down OPT+INS for at least two seconds until the device beeps again
twice!
Purpose: Obligatory initializing of the non-volatile program memory and of the non-
volatile calibration memory:
All programs and calibration data are reset.
This should be carried out when unusual messages / error messages (Internal
Err xxx) appear in the display and when there are doubts about the calibration
data.
The device must then be recalibrated!
MSTM/MMWT/ 1301 Alignment/Adjustment 5-1

Main menu Software version < 2.0:
Clock Remote Sound Etc Serv
Service menu:
Block Lid Sensors Div
Display of all
temperature sensors
Block menu:
Params Outputs Calibr TChanges
Input of Output Temperature Temperature-

control parameters of Block sensor change
Input of PW control parameters Calibration counter
Input of block
parameters
LID menu:
LidIn Calibr
Input and Temperature

output of control Lid sensor
parameters Calibration
Input of LID
parameters
DIV menu:
Keydb* Fans Inits Pelt Mcard***
Display Printout of Direct control of Memory

of Fan tests initializing of the Peltier card
keycodes internal parameters Calculation of PW test
* For Mastercycler 5331 / 5333 / 5334.
5-2 Alignment/Adjustment MSTM/MMWT/ 1301

Main menu from Software version 2.0 onwards:
Clock Remote Sound Etc Serv
Service menu:
Block Lid Sensors Div Errors
Display of all Error Log

temperature sensors The Error Log
can be printed
by using the
Opt. key
Block menu:
Params Outputs Calibr TChanges
Input of Output Temperature Temperature-

control parameters of Block sensor change
Input of PW control Calibration counter
parameters Input of block
parameters
LID menu:
LidIn Calibr
Input and Temperature

output of control Lid sensor
parameters Calibration
Input of LID
parameters
DIV menu:
Fans Inits Peltier Mcard*
Printout of Direct control of Memory

Fan tests initializing of the Peltier card
internal parameters Calculation of PW test
* For Mastercycler 5331 / 5333 / 5334.
MSTM/MMWT/1301 Alignment/Adjustment 5-3

Calculating the Peltier current limitation (PW)
Utilities: Multimeter 3 1/2-digit, range DC 10 A

Cable for measuring adapter 5332 625.006 (for personal only)
It is essential to switch off the AutoRestart function prior to calling up this function!
Go to: Main-Menu-Options-Generals-Etc.-AutoRestart.
Switch function OFF using the SEL key.
With this function switched ON, a program which has not been terminated continues to run after
the device has been switched on. This means that the thermoblocks are under current.
With the Mastercycler 5332, a PW must be calculated. With the Mastercycler 5331 / 5333 / 5334, a PW
must be calculated for the left-hand, middle and right-hand block respectively. The PW (impulse width)
must be calculated each time a thermoblock or PCB Power Unit is attached. The PW depends on the
power unit used. This function can be used to set the Peltier current limitation (PW) to 5,000 A ±10 mA.
PW limits the maximum control output and thus restricts the Peltier current.
Initial experiences have shown that the PW values is between 173 and 195.
The initial PW should be –180, so that the cycler cools down instead of heating up.
– Switch the multimeter to DC and range 10 A.
– Switch the measuring adapter cable or the multimeter between the Peltier element.
– Call up the service function.
– Call up DIV ... and confirm by pressing Enter.
– Call up the Pelt function.
Important!
This function must be monitored by the user at all times.
– The power supply can be interrupted immediately by pressing the Exit key.
The polarity (direction of the current through the Peltier element) can be modified using the ± key:
+ signifies heating
– signifies cooling.
---------------Peltier--------------------------
PeltOut= +0/ +0
24.9 °C (room temperature)
Example:
The function shows a room temperature of 24.9 °C.
– Enter –180 to enable the Mastercycler to cool down.
– If the Enter key is pressed, a current (see multimeter) of approximately 5 amps starts, and the
temperature drops (see display of Mastercycler).
– Wait until the temperature has dropped to approximately 15 °C and then press the ± key followed by
the Enter key. The polarity is reversed and the Peltier element begins heating.

– Carefully monitor the temperature curve: When a temperature of 25 °C has been reached, the
current should be exactly 5,000 ±10 mA.
– If this is not the case, the PW must be modified.

This can be carried out when the device is in operation. Modify the PW and confirm by pressing
Enter.
If the current is too low, increase the PW; if it is too high, reduce the PW.
Summary:
°C
Press the Plus/
40 ± Minus key to
switch from
heating to cooling
25
t
ar
St
15
In the case of a temperature curve of 15 °C to
± 40 °C and vice-versa, a current of 5,000 ±10 mA
Press the Plus/
Minus key to must be set when a temperature of 25 °C is
switch from
reached.
heating to cooling
0 If this does not occur, the PW must be modified.
5.000 A ±10 mA
If a temperature of 110 °C is exceeded, an error message appears and the power supply is cut off for
safety reasons.
The setting can be reactivated by pressing the Exit key and calling up the function again.
Storing the Peltier current limitation (PW)
The PW which has been calculated must be entered in the BLOCK Params function and is stored when
the function is exited (by pressing the Exit key).
MSTM/MMWT/1301 Alignment/Adjustment 5-5

When replacing the thermoblock and/or the heated lid, please observe the following:
Replacing the heated lid
The parameter list does not need to be printed out in this case, as all heated lids have fixed parameters.
For the gradient heated lid is valid for lid segment no.: 0 Stemp1 = 502 (50.2)
Stemp2 = 1060 (106.0)
For the personal heated lid is valid for lid segment no.: 0 Stemp1 = 503 (50.3)
Stemp2 = 1057 (105.7)
and for lid segment no.: 1 Stemp1 = 500 (50.0)

Stemp2 = 1040 (104.0)
These parameters are entered via service function Serv – Lid – Calibr.
Replacing the thermoblock
The "Temp. Changes of Block" and the serial number of the faulty thermoblock must be noted on
the SIL.
First of all, after the thermoblock has been replaced, the PW must be determined.
This is done via service function: Serv – Div – Pelt – Calculation of PW.
The PW needs to be determined 1x for 5332 and 3x for 5331 / 5333 / 5334!
PW(s) can be entered via service function: Serv – Block – Params – Input of PW.
The corresponding heated lid-parameters are then entered (see above).
The parameters for the new thermoblock and the heated lid are stored in the EEPROM on the small PCB
on the thermoblock.

Checking the sensor functions
Service function "Sensors" allows to display all sensors:
Mastercycler 5332 Mastercycler 5331 / 5333 / 5334
Sensor Sensor
0 – B – Thermoblock 0 – L – Thermoblock
1 – L – Lid (top) 1 – M – Thermoblock
2 – l – Lid (lid frame) 2 – R – Thermoblock
3 – C – Thermoblock (cooler) 3 – l – Lid (top)
4 – C – Thermoblock (cooler)
If a sensor is defective and the display shows – – – instead of a temperature of e.g. 25.0 °C, the
corresponding sub-assembly must be replaced.
Procedure for establishing the degree of aging of the Peltier module
In the course of its service life, the thermal effectivity of the Peltier module decreases. This causes a
general slowing-down of the device, which is most noticeable by the deterioration of the cooling
performance. The degree of aging can be determined with the aid of a simple program:
Lid = 105 °C, Nowait

1 T = 95 °C, 5 min
2 T = 95 °C, 10 sec
3 T = 20 °C, 10 sec
4 GO TO2, REP 1
5 Hold 95°C enter
6 T = 95 °C, 10 sec
7 T = 20 °C, 10 sec
8 GO TO6, REP 4
9 Sound 5
end
The program lasts for approximately 25 minutes. Steps 1 to 5 set the device to a defined thermal state, if
it is not already warm from operation. The evaluation criteria for the state of the Peltier module are the
duration of steps 6 and 7 of the cycle as well as the synchronization of the three block segments with
5331 / 5333 / 5334.
The temperature curves of the three blocks must be synchronized, i.e. they must be virtually identical at
all times. Some temporary deviations can be expected due to the controller action when the temperature
is within 1 °C of the nominal temperature. This can be seen in the Service program "Sensors" during the
initial two switches between step 6 and step 7.
MSTM/MMWT/ 1301 Alignment/Adjustment 5-7

The last two periods should be used as a basis for determination of the duration of the period. This is
best achieved by observing the program sequence in the display and stopping the time between two
cycles which successively follow two identical program points. One way of doing this is by using the end
of the lag-time at 20 °C or 95 °C. Times > 180 seconds indicate wear and tear on the module. The
specifications are valid at room temperature; higher ambient temperatures prolong the times marginally.
The cycle for testing the aging of the device is approximately 120 seconds long with the
Mastercycler 5332.
There is a new temperature validation system.

You can find further information in the "Operating instructions for the temperature validation system", you can
download as pdf-file on the www.eppendorf-support.com.You can find additional order numbers in Chapter 7 of
this service manual.

8 7 6 5 4 3 2 1
BL BL
5331
F NETZ CARD READER KUEHLUNG ELEKTRONIK
MAIN LP"TASTENFELD" 24V/8W 24V/2,4W
X6
6 3 CARD
T 5.0 A 230V
T 6,3 A 115V
MT 8,0 A 100V
VIO
4 1
M M
VIO
X4 PRINTER X15 RS 232
NETZMODUL
X7 X5 X6
GN GN 10 pol TB 11p. STIFT 25p. SUBD-BU 9p. SUBD-ST
TRAFO
8p. AMI X3
230V GN RESERVE 16 pol. ODU
E SW
NEUTRAL
X22 LCD-ANZEIGE
LINE
W1
8,5/2A LED A GRAHPIC DISPLAY
GND C
GN LP"RECHNER2-G,KPL." X23
VIO
GEH. LEISTUNG_BL.
W4 RT1
GEH. TRAFO-BL.
13,5V/0,5A
KONTRAST
VIO
LP"RECHNER2-G"
115V GE Bl
W2
D 22,3A/2A POWERUNIT
100V VIO BL X21
rt
sw
rt
sw
34p. TB
RT
CONFIDENTIAL
X16
VERTRAULICH
100/115V
ZEP X21
ZEP W3 6p. MMNL 34p. TB 2pol.AMI
TEMP-SICH.
230V
26V/19A
1 F3 T2,0A X2+3 X19 X20
WS RT 4 5V VERSORGUNG X22
X4+5
UNIVERSAL-TRAFO
2
5
F1 FF0,75A
15V VERSORGUNG X23 LP"LEISTUNGSEINHEIT-M"
N1
N L X6+7
L1
L2
SL TRAFO (ca.562 VA) 3 F4 T3,0A X24

HEIZUNG+LUEFTER
6
C NETZFILTER
B80/70-25
F5 FF10A F6 FF10A F7 FF10A
MAIN POWER FILTER X17 F2
CORCUM 6EXP PELTIER VERS. PELTIER_L PELTIER_M PELTIER_R
T25A (PICOFUSE)
X18 X8 X9 X10 X11 X12 X13
SG59 (230V) BORD HEAT
SG38 (115/100V) X14
KUEHLKOEPER
STROM- X27
BEGRENZUNG 16p. TB 10p. FLC HEIZFOLIE
1 2 1 2 1 2 FOLIE 1
X14
16p. TB X8 X9 X12 X13
LP"THERMOBLOCK" X10 X11 Heizung
P-out_L
P+out_L
P-out_M
P+out_M
P-out_R
P+out_R
MASSE
B 2p.MNL
O1
6,3 mm Faston
P51
P52
P61
P62
P31
P32
P41
P42
P21
P22
P11
P12
Temp.Sensor
V1
S1
G1
V2
S2
G2
V3
S3
G3
- + - + - + - + - + - +
rt
rt
rt
rt
rt
rt
SMT160
SENSOR AUF
sw
sw
sw
sw
sw
sw
VCC
GND
VCC
GND
VCC
GND
TEMP
TEMP
TEMP
3 2 1
KUEHLBLOCK
SMT-160 MONTIERT GND VCC OUT
A
UEBERSICHTSPLAN-MASTER
SMT-160
SMT-160
SMT-160
For service purposes only

PELTIER 1 PELTIER 1 PELTIER 1 GENERAL CIRCUIT DIAGRAMM
Blatt 1 von 1
BLOCK L BLOCK M BLOCK R eppendorf 5331_900.020-02

8 7 6 5 4 3 2 1
5332
F PELT-FAN
CARD READER 24V/7W INT-FAN
LP"TASTENFELD" 24V/2,6W
CARD
M M
PRINTER RS 232
X7 X5 X6
TRAFO (ca.249 VA) X4 X15
10 pol TB 11p. STIFT 25p. SUBD 9p. SUBD
8p. AMI X3
230V L1 230V GN RESERVE 16 pol. ODU
E SW
BEGRENZUNG
W1 X22 LCD-ANZEIGE
STROM-
L2 115V GE
LP"NETZFILTER 115V/230V"
LED A
8,5/2A
LP"RECHNER-P,KPL."
OPTION
L3 100V GND C
VIO GN 4 X20 Zeichen
100/115V X23
VIO
MAIN POWER FILTER
N2
W4 RT1
NETZFILTER
N1 13,5V/0,5A LP"RECHNER-P"
CORCUM
3EYP
KONTRAST
K2 VIO
K1 Bl
W2
D 22,3A/2A POWERUNIT
L N SL
BL X21
rt
sw
rt
sw
34p. TB
RT
CONFIDENTIAL
X1
VERTRAULICH
X10
W3 6p. MMNL 34p. TB 2,8 mm Faston
TEMP-SICH. 26V/19A
1 F3 T1,5A X19 X22 X16 X17
WS RT 4 5V VERSORGUNG
2
5
F1 F0,5A
14V VERSORGUNG
LP"LEISTUNGSEINHEIT-P"
UNIVERSAL-TRAFO 3 F4 T2,0A
PELTIER-VERS.
TRAFO (ca.249 VA) 6
C Masse
X13 F2 PELTIER 15V-VERS. PELTIER
Strombegr. X14 FF10A X6 X7
Masse X8 20p. TB
HEIZFOLIE-P
X0 FOLIE 1 2 1
20p. TB X5 X7
10p. FLC 2pol.MNL
BORD HEAT
Heizung - +
NEUTRAL
LINE
ZEP BL BL HEIZFOLIE-M
LP"SENSOR + MULTI."
B
NETZMODUL
O1 FOLIE 2
rt
sw
sw
X6 Temp.Sensor
T 3,15 A 115V
T 3,15 A 100V
X6
LID HEAT
T 2,0 A 230V
6 3 10p. FLC Heizung

S1
S2
S3
VIO
4 1 SMT160
O1
VIO SENSOR AUF 3 2 1
VCC
GND
TEMP
KUEHLBLOCK Temp.Sensor
GND VCC OUT
MONTIERT
GN GN
MAIN
A NETZ
UEBERSICHTSPLAN-PERS.
SMT-160-HE
For service purposes only

GENERAL CIRCUIT DIAGRAMM
Blatt 1 von 1
eppendorf 5332 900.112-02
7 Spare parts list
Mastercycler 5332
Keypad, new design
1-5 1-4 2-1 1-2 Fig. 1
MSTM/MMWT/4711 Spare parts list 7-1

7 Spare parts list
Mastercycler 5332
3-2
3-1
3-6 Fig. 2
7-2 Spare parts list MSTM/MMWT/ 4711

7 Spare parts list
Mastercycler 5332
1-5 2-4 5-1
1-6 2-7 1-1 Fig. 3

7 Spare parts list
Keypad 5331, new design
1-5 1-4 2-1 1-2 Fig. 4

7 Spare parts list
Heated lid
5331 / 5333
3-2
Thermoblock
5331 / 5333
3-1
Fig. 5

7 Spare parts list
5-1 3-6 2-4 1-7
Fig. 6 1-1 1-5 3-7 1-6
7-6 Spare parts list MSTM/KW/4711

MSTM/MMWT/
7 Spare parts list
Fig. Pos. Designation New part Pool part
31
32
33
34
1 Housing ---------------
6 1-1 Lower part of housing, incl. feet x x x 5331 853.005
3 1-1 Lower part of housing, incl. feet x 5332 853.009
4 1-2 Upper part of housing x x 5331 875.009
1 1-2 Upper part of housing x 5332 869.002
1 1-5 Keypad x 5332 870.000
4 1-5 Keypad x 5331 876.005
4 1-5 Keypad x 5333 856.001
1-3 Device feet (set of 4) x x x x 5332 852.002
4 1-4 Display x x x 5331 860.001
1 1-4 Display x 5332 860.005
6 1-5 Universal toroidal transformer x x x 5331 852.009

for 100, 115, 230 Volt
3 1-5 Universal toroidal transformer x 5332 854.005

for 100, 115, 230 Volt
6 1-6 Mains input module x x x 0013 601.259
3 1-6 Mains input module x 0013 607.958
6 1-7 Bridge rectifier x x x x 0013 530.106
2 PCBs ---------------
4 2-1 PCB Computer with flashloader x x x 5331 870.007

Universal 31 / 33 / 34 + Software
(without display)
1 2-1 PCB Computer with EPROM  x 5332 800.002

(without display)
2-2 Software update (EPROM version) x 5332 867.000
2-3 Zero modem for update/CycleManager x x x x 5331 867.006
6 2-4 PCB Power Unit x x x 5331 866.000

for 100, 115 and 230 Volt
3 2-4 PCB Power unit x 5332 861.001

for 100, 115 and 230 Volt
2-5 RAM module x x x x 0013 591.458
2-6 Socket with battery x x x x 0013 604.592
MSTM/KW/4711
MSTM/MMWT/ Spare parts list 7-7
7 Spare parts list
31
32
33
34
3 2-7 PCB Line filter for 100,115, 230 Volt x 5332 808.003
2-8 PCB Interference choke x 5332 815.000
3 Thermoblock / Heated lid ---------------
5 3-1 Thermoblock for 5331 / 5333 x x 5331 891.004 2223 031.504
2 3-1 Thermoblock for 5332 x 5332 851.006 2223 032.004
5 3-2 Heated lid for 5331 / 5333 x x 5331 864.007
2 3-2 Heated lid for 5332 x 5332 855.001
3-3 Lid frame incl. Velcro x x x 5331 856.004
3-3 Lid frame incl. Velcro x 5332 856.008
3-4 Velcro fastenings (set) x x x 5331 858.007
3-4 Velcro fastenings (set) x 5332 859.007
3-5 Air guide (left and right) x x x 5331 859.003
6 3-6 Fan, large x x x 5331 602.002
2 3-6 Fan, large x 5332 604.009
6 3-7 Fan, small x x x 5331 601.006
3-8 Repair set of knob for Heated lid x x x x 5331 868.002
3-9 Mux-cable x x x 5331 874.002
4 Fuses ---------------
4-1 Fuse for 230 Volt, slow 2 A x 0013 608.130
4-2 Fuse for 100/115 Volt, slow 3.15 A x 0013 609.098
4-3 Fuse for 230 Volt, slow 5.0 A x x x 0013 609.349
4-4 Fuse for 115 Volt, slow 6.3 A x x x 0013 565.333
4-5 Fuse for 100 Volt, semi-lag 8 A x x x 0013 611.432
4-6 Soldered fuse 0.5 A, slow x 0013 606.072
4-7 Soldered fuse 0.75 A, high-speed x x x 0013 609.039
4-8 Soldered fuse 1.5 A, slow x 0013 608.695
4-9 Soldered fuse 2.0 A, slow x x 0013 608.709
4-10 Soldered fuse 3.0 A, slow x x x 0013 612.889

(Heated lid, fast version)
4-11 Soldered fuse 10 A, high-speed x x x x 0013 608.300

7 Spare parts list
31
32
33
34
4-12 Soldered fuse 25 A, fast x x x 0013 609.004
5 Auxiliary aids ---------------
3/6 5-1 Chip-card reader x x x x 0013 607.800
5-2 Set of labels x x x x 5332 858.000

(2x Hot Surface / 1x Caution)
5-3 Measuring-adapter cable  x 5332 625.006

(for PW determination)
5-4 Temperature Verification System x x x 0055 000.298
5-5 Temperature sensor for 0.2 ml x x x 5341 897.000
5-6 Adapter galvanic separation with power x x x x 5341 898.007

supply
5-7 Power supply for testodevice (230 only) x x x x 5341 899.003
5-8 Zero Modemadapter (for the tempera- x x x x 5341 222.003

ture validation measuring device)
5-9 Connecting cable x x x 5331 229.026

for temperature sensor
5-10 RS 232 C connecting cable x x x x 5331 229.018
5-11 Personal card x x x x 5331 238.009
5-13 Sensor 348 well x 5331 233.007
5-14 Silicone mat for 384er sensor only x 5331 234.003
5-15 Chip card 384 x 5331 235.000
5-16 Packaging x 5332 868.006
5-17 Packaging x x x 5331 869.009
Accessories: See operating manual for Mastercycler 5332 5332 900.015

Mastercycler 5331 / 5333 5331 900.011
Mastercycler 5334 5334 900.101
MSTM/KW/4711
MSTM/MMWT/ Spare parts list 7-9
7 Spare parts list
Recommended spare parts – Initial delivery
To: Eppendorf AG From:
Fax no.: 040 53801758 Fax no.:
Name:
Tel.:
Order no.:
Qnty Fig. Pos. Designation New part
31
32
33
34
4 1-4 Display x x x 5331 860.001
1 1-4 Display x 5332 860.005
4 2-1 PCB Computer with flashloader Universal x x x 5331 870.007

31 / 33 /34 + Software (without display)
6 2-3 PCB Power unit for 100, 115 and 230 Volt x x x 5331 866.000
3 2-3 PCB Power unit for 100, 115 and 230 Volt x 5332 861.001
5 3-1 Thermoblock for 5331 / 5333 x x 5331 891.004
2 3-1 Thermoblock for 5332 x 5332 851.006
5 3-2 Heated lid for 5331 / 5333 x x 5331 864.007
2 3-2 Heated lid for 5332 x 5332 855.001
6 3-6 Fan, large x x x 5331 602.002
2 3-6 Fan, large x 5332 604.009
6 3-7 Fan, small x x x 5331 601.006
3/6 5-1 Chip-card reader x x x x 0013 607.800

8 Technical data
Mastercycler® 5331 and 5333
Sample capacity: 1 PCR plate

or 96 0.2 ml PCR tubes
or 77 0.5 ml PCR tubes, thin-walled
Temperature range: 4 to 99 °C
Temperature uniformity across the block: 20 to 72 °C ± 0.6 K
95 °C ± 1.0 K
Block homogeneity (S95): 20 to 72 °C ± 0.4 K
95 °C ± 0.5 K
Control accuracy: ± 0.2 K
Temperature-control speeds: Heating rate: approx.3 K/s, measured on the block

Cooling rate: approx. 2 K/s, measured on the block
Number of programs: max. 99
max. 10 on a personal card
Maximum number of cycles: 99

Dimensions: Width: 26 cm
Depth: 41 cm
Height: 27 cm
Weight: approx. 12.4 kg
Voltage/frequency: 230 V, 50–60 Hz 115 V, 50–60 Hz 100 V, 50–60 Hz
Power requirement: 500 W 500 W 500 W
Operating current: 2.6 A 5A 5A
Fuses: 2x 5 A slow, 2x 6.3 A slow, 2x 8 A semi-lag,
250 V 250 V 250 V
Overvoltage category: II
Pollution degree: 2
Contamination level: I
Operating conditions: 15 to 35 °C, up to 70 % rel. humidity
Storage conditions: –20 to 70 °C, up to 85 % rel. humidity
The device is -approved, fullfills UL 3101-1 und CSA C 22.2 Nr. 1010.1.
Technical specifications subject to change!
MSTM/MMWT/ 1301 Technical data 8-1

8 Technical data
Mastercycler® 5332
Sample capacity: 1 microtiter plate

or 25 0.2 ml PCR tubes
or 16 0.5 ml PCR tubes, thin-walled
Temperature uniformity across the block: 95 °C ± 1.0 K
20 to 72 °C ± 0.6 K
Temperature-control speeds: Heating rate: approx. 3 K/s, measured on the block
Depth: 35 cm
Height: 25 cm

Voltage/frequency: 230 V, 50–60 Hz 115 V, 50–60 Hz 100 V, 50–60 Hz
Power requirement: 150 W 150 W 170
Operating current: 0.8 A 1.6 A 2.1 A
Fuses: 2x 5 A slow, 2x 3.15 A slow, 2x 3.15 A slow,
250 V 250 V 250 V
Pollution degree: 1
The device is -approved, fulfills UL 3101-1 und CSA C 22.2 Nr. 1010.1.
8-2 Technical data MSTM/MMWT/ 1301

8 Technical data
Mastercycler® 5334
Sample capacity: 1 PCR plate 384

Temperature uniformity across the block: 20 to 72 °C ± 0.5 K
95 °C ± 0.9 K
Block homogeneity (S95): 20 to 72 °C ± 0.3 K

95 °C ± 0.4 K
Temperature-control speeds: Heating rate: approx. 3 K/s, measured on the block

Depth: 41 cm
Height: 27 cm
Voltage/frequency: 230 V, 50–60 Hz 115 V, 50–60 Hz
Power requirement: 500 W 500 W
Operating current: 2.6 A 5A
Fuses: 2x 5 A slow, 2x 6.3 A slow,
250 V 250 V
Pollution degree: 2

The device is -approved, fulfills UL 3101-1 and CSA C 22.2 No.. 1010.1.
MSTM/MMWT/ 1301 Technical data 8-3

8 Technical data
8-4 Technical data MSTM/MMWT/ 1301

9 Maintenance
KS/1109 Maintenance 9-1

Group 1 Group 2 Group 3
Classic Cycler Mastercycler ep and pro Mastercycler ep realplex
Maintenance of PCR Systems 5331 5332 5333 5334 5341 5344 5345 6300 6302
6321 6324 6325
Nr. Completion recommended every months: 12 12 12 12 12 12 12 12 12
M 01 Cleaning the device according to instruction manual x x x x x x x x x
M 02 Analyze device data and write down if necessary x x x x x x x x x
M 03 Keyboard x x x x x x x
M 04 Visual inspection of display x x x x x x x
M 05 Stability x x x x x x x x x
M 06 Cleaning the internal assemblies x x x x x x x x x
M 07 Housing x x x x x x x x x
M 08 Printed circuit board/current supply x x x x x x x x x
M 09 Heated lid x x x x x x x
M 10 Heated lid for Mastercycler pro x x x
M 11 Thermoblock x x x x x x x x x
M 12 Motor fan/device fan x x x x x x x x x
M 13 realplex module x x
M 14 Software update x x x x x x x x x
M 15 Conduct system test x x x x x x x x x
Average time expenditure 2.5 to 3 hours 2 to 2.5 hours 3 to 3.5 hours
The indicated times are only valid for devices that are not more than four years old.
For older devices, the time expenditure can be higher.
Spare parts which may be required are not included in the expenditure.
For the models 5331/32/33/34, a thermal validation is calculated with software version 2.1 or higher.
For the models 5331/33/34, a software update is only calculated with empty internal memory and for systems without EPROM exchange.
KS/1109
Operational Qualification (OQ) Classic Cycler Mastercycler ep and pro Mastercycler ep realplex
including electrical test 5331 5332 5333 5334 5341 5344 5345 6300 6302
6321 6324 6325
Completion recommended every months: 12 12 12 12 12 12 12 12 12
Operation Qualification (OQ) including electrical test x x x x x x x x x
Average time expenditure 2 hours 2 hours 2 hours

Extra Maintenance Service Classic Cycler Mastercycler ep and pro Mastercycler ep realplex
of PCR Systems 5331 5332 5333 5334 5341 5344 5345 6300 6302
6321 6324 6325
ME 01 Software upgrade x x x x x x x x x
Average time expenditure 15 minutes 10 minutes 30 minutes

ME 02 Electrical test x x x x x x x x x

ME 03 Thermal validation x x x x x x x x x

ME 04 Background Calibration x x
Average time expenditure 15 minutes

ME 05 Color Calibration (incl. Background Calibration) x x
Average time expenditure 60 minutes
The indicated times are only valid for devices that are not more than four years old.
For older devices, the time expenditure can be higher.
Spare parts which may be required are not included in the expenditure.
For the models 5331/32/33/34, a thermal validation is calculated with software version 2.1 or higher.
For the models 5331/33/34, a software update is only calculated with empty internal memory and for systems without EPROM exchange.
KS/1109
9 Maintenance
Description: Maintenance of PCR Systems
M 01 Cleaning the device according to instruction manual

Clean PCR systems as described in the respective operating manual and supplement sheets.
M 02 Analyze device data and write down/delete if necessary

(Note: The individual parameters vary, or are not available, based on the PCR system.)
Analyze the following parameters with the service program:
– Error statistics
– Number of cycle counts
– Number of pulse counts
– Number of program counts
M 03 Keyboard
Check the keyboard by pressing all keys.
M 04 Visual inspection of display

Check the display with the corresponding service program ( if available).
When doing so, pay special attention to the following points:
– Defective pixels
– Background lighting
M 05 Stability
Check the stability of the device feet.
They must be replaced if fissures or damages are present.
M 06 Cleaning the internal assemblies

Disassemble/assemble the PCR systems as described in the respective service manual and
supplement sheets.
M 07 Housing
Remove dust and soiling with a cleaning brush, or, if necessary, compressed air.
Pay attention to damages that compromise safety.
With existing fissures, there is the possibility of unintentional infiltration of liquids. If this occurs, the
housing must be replaced.
M 08 Printed circuit board / current supply:

M 09 Heated lid
Clean dust and soiling with a cleaning brush, or, if necessary, compressed air.
Clean the slide rails for the heated lid with a lint-free cloth.
M 10 Heated lid for MC pro

Inspect the flexible pressure element of the vapo protect heated lid for damages.
M 11 Thermoblock
When cleaning a realplex thermoblock, the cleaning procedure described in the instruction manual
must be observed.
M 12 Motor/device fan:
Pay attention to operating noise.
With the service program, select various voltages for testing. ( if available )
9-4 Maintenance KS/ 1109

9 Maintenance
M 13 realplex module
Clean the frame with heating foil, insulating plate, and lower lens array as described in the
respective service manual.
M 14 Software update
Install the latest software in order to utilize the product improvements offered by new functions.
The latest software is available for download at www.eppendorf-support.com.
M 15 Conduct system test

After the maintenance, we recommend conducting an OQ, including the test for electrical safety.
Description: Extra Maintenance Services of PCR systems
ME 01 Software upgrade
Install the latest software (available for a fee) in order to utilize the product improvements offered by
new functions.
The latest software is available for download at www.eppendorf-support.com.
ME 02 Electrical test
See SOP for PCR systems.
ME 03 Thermal validation
For the temperature test, use the Eppendorf temperature validation set 0055 000.298.
ME 04 Background Calibration
See Operating manual Mastercycler ep realplex.
ME 05 Color Calibration (incl. Background Calibration)

See Operating manual Mastercycler ep realplex.
KS/ 1109 Maintenance 9-5

9 Maintenance
Electrical safety test
Effective standard
IEC 61010-1 (complies with EN 61010-1)
Measuring device
The specifications of the above-mentioned standard must be able to be measured using the measuring device.
Consult the manufacturer of your measuring device.
Only a properly instructed and duly qualified electrician may carry out electrical safety tests.
Notes concerning the measuring procedure

Measurement is carried out in accordance with EN 61010-1.
Measurement pursuant to any other, country-specific standard (e.g. VDE 701/702) will be allowed only if this
standard specifies the same measuring procedures and limiting values and is subordinated to EN 61010-1.
If the measurement is carried out in accordance with any other standard, attention must be paid to the fact that the
measuring arrangement meets the requirements of EN 61010-1.
In the US, the PE conductor current is measured in accordance with NFPA 99.
Handling of the different measuring devices may vary.
Consult the operating manual of the measuring device for information about the measuring procedure.
Measurement is carried out on a grounded, metallic point that is not insulated and can be touched from the
outside (e.g. housing screws).
Never use an optionally provided interface for measurement. You might damage the electronic system of the
device.
9-6 Maintenance KS/ 1109

IQ – Installation Qualification OQ – Operational Qualification PQ – Performance Qualification
for Eppendorf Mastercycler gradient 5331, Mastercycler personal 5332, Mastercycler 5333 and
Mastercycler 384 5334.
• This document is Eppendorf AG’s proposal for IQ/OQ/PQ.

• Only a trained service technician may install and commission the instrument and conduct the tests.
• Only the latest updated version of the Service Manual is applicable.
• The current operating instructions enclosed should be kept handy and used to operate the instru-
ment.
KS/1109 Check/Inspection 10-1

IQ – Installation Qualification
Installing and commissioning Mastercycler gradient 5331, Mastercycler personal 5332,

Mastercycler 5333 and Mastercycler 384 5334.
1. Check condition of transport packaging

Yes No
Transport packaging is free of damage
2. Check scope of delivery

The label on the transport packaging shows information on the content.
Check whether the information on the label corresponds with the ordered version .
Was the right model delivered Yes No
Mastercycler gradient 5331
Mastercycler personal 5332
Mastercycler 5333
Mastercycler 384 5334
Which voltage version was delivered Yes No

230 Volt, 50 – 60 Hz
115 Volt, 50 – 60 Hz
100 Volt, 50 – 60 Hz
Yes No
The information on the label correspond with the ordered version
3. Check stability of work surface before installing the device
Note:
Weight of Mastercycler gradient 5331, Mastercycler 5333, Mastercycler 384 5334:12.4 kg.
Weight of Mastercycler personal 5332: 6.8 kg.
Yes No
Stability of work surface is sufficient
10-2 Check/Inspection KS/1109

4. Unpacking the Mastercycler

Proce- Description Yes No
dure
4.1 Open transport packaging.
4.2 With Mastercycler gradient 5331, Mastercycler 5333 and
Mastercycler 384 5334, remove upper part of inner foam parts.
4.3 Take out Mastercycler and put on work surface.
4.4 Take out Mastercycler personal 5332 together with inner packaging and put on
work surface.
4.5 Remove both foam parts.
4.6 Remove adhesive strips from heating lid.
4.7 Disengage and open heating lid. Remove bubble-wrap from below heated lid.
Note.
Keep the delivery box and transport packaging in case any transport is required.
Use the original transport packaging for shippings to the manufacturer.
5. Check scope of supply

Quantity Description Yes No
1 Mastercycler:
1 Mastercycler gradient 5331
1 Mastercycler 5333
1 Mastercycler 384 5334
1 Mastercycler personal 5332
1 Was the correct version of mains cable delivered
1 Operating instructions
1 Personal card
Set of fuses for Mastercycler gradient 5331, Mastercycler 5333, Mastercycler 384 5334:
1 230 Volt, 2x time lag 5 A, 250 V
1 115 Volt, 2x time lag 6.3 A, 250 V
1 100 Volt, 2x semi time lag 8 A, 250 V
Set of fuses for Mastercycler personal 5332:

1 230 Volt, 2x time lag 2 A, 250 V
1 115 Volt 2 x time lag 3.15 A, 250 V
1 100 Volt, 2x time lag 3.15 A, 250 V
For Mastercycler gradient 5331, Mastercycler 5333 and Mastercycler 384:

1 Pouch PCR tubes 0.2 ml (100 pcs.)
1 Pouch PCR tubes 0.5 ml thin-walled (100 pcs.)
2 twin.tec PCR plate 96 (red / blue skirted)
For Mastercycler personal 5332:

1 Pouch PCR tubes 0.2 ml (100 pcs.)
1 Pouch PCR tubes 0.5 ml thin-walled (100 pcs.)

6. Set up instrument
Note:
In order to avoid damage caused by condensation, the device may be switched on 12 hours after setting
up. This also applies to a change of location.
Space required for Mastercycler personal 5332: width 18 cm, depth 35 cm, height 25 cm.
Check that there is a safety distance of 30 cm around the Mastercycler.
Do not expose the Mastercycler to direct sunlight.
dure
6.1 Visual inspection, Mastercycler has not suffered any transport damage.
6.2 Check horizontal position of laboratory bench.
6.3 Check mains voltage in the lab (± 10 %) and compare with ID plate of
Mastercycler.
6.4 Frequency values ± 1 Hz in the lab, compare with ID plate of Mastercycler.
6.5 Connect mains cable, press ON switch.
7. Entries pertaining to the instrument
Location of Mastercycler gradient 5331, Mastercycler personal 5332, Mastercycler 5333 and
Mastercycler 384: (building, floor, laboratory etc.):......................................................................................
.....................................................................................................................................................................
Model: .................................................................. Serial number:......................................................
Power supply in volts: .......................................... Frequency in Hz: ..................................................
Customer’s inventory number:.....................................................................................................................
Date of installation, day/month/year: ...........................................................................................................
Location of operating instructions: ..............................................................................................................

8. Operating the instrument
This form can be used to show the customer the operation of Mastercycler gradient 5331,
Mastercycler personal 5332, Mastercycler 5333 und Mastercycler 384 5334.
Note:
Perform the following operating steps following the corresponding operating instructions.
For Mastercycler 5331, see operating instructions 5331 900.062
For Mastercycler 384 5334, see operating instructions 5334 900.101

dure
8.1 Switch on device.
8.2 Device type and software version is displayed.
8.3 Check keypad.
8.4 For Mastercycler gradient 5331, Mastercycler 5333 and
Mastercycler 384 5334:
set brightness of display.
8.5 Distribute at least 4 – 6 PCR test tubes over the complete block and check
whether the the heated lid locks in the corresponding position (0.2ml or 0.5
ml).
8.6 Check locking of heated lid with twin.tec plates as well.
8.7 Set temperature of heated lid to approx. 105 °C.
8.8 Connect printer if neccessary.
8.9 Activate printer.
8.10 Call up "Standard" program.
8.11 Show programming (editor function). Show block and tube control.
8.12 Start program.
8.13 Check printer protocol.
8.14 Stop program after approx. 10 min.
8.15 Discuss section troubleshooting.
8.16 Format personal card.
8.17 Check personal card.
8.18 Save program to personal card.
8.19 Switch off device.
Customer ................................................................................... ................................ has been instructed.
Remarks:......................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
Signature of technician ........................................ Date: ................................................................... .
Signature of customer ......................................... Date: ....................................................................

OQ – Operational Qualification
Checking the performance data of the Mastercycler.
9. Performance data of
Mastercycler 5333
Mastercycler 384 5334
Serial number:
Values in
specification
dure
9.1 Accessible current < 3.5 mA
9.2 Accessible current < 0.5 mA (applicable only to USA as per NFPA 99)
9.3 PE <= 0.1 E according to IEC 61010-1 (<= 0.3 E according to VDE 702-1)
9.4 Insulation resistance > 0,5 ME
9.5 Checking the temperature
For Mastercycler gradient 5331, Mastercycler personal 5332,
Mastercycler 5333, Eppendorf offers the Temperature Validating System
0055 000.298
For Mastercycler 384 5334, Eppendorf offers the Temperature Sensor
5331 233.007 for Temperature Validating System 0055 000.298.
9.7 Noise level < 70 dB (A)
Remarks: ......................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
Signature of technician ........................................ Date: ....................................................................
Signature of customer.......................................... Date: ....................................................................

PQ – Performance Qualification
Periodic check on performance data of Mastercyclers.
After maintenance or repair work, the operating check needs to be repeated to ensure compliance with
the manufacturer’s specifications. Recertification is required following repairs or modifications.
10. Performance data of

Mastercycler 5333
Mastercycler 384
Serial number:
Values in
specification
dure
10.1 Accessible current < 3.5 mA
10.2 Accessible current < 0.5 mA (applicable only to USA as per NFPA 99)
10.3 PE <= 0,1 E according to IEC 61010-1 (<= 0,3 E according to VDE 702-1)
10.4 Insulation resistance > 0,5 ME
10.5 Carry out procedure for establishing the degree of aging of the peltier ele-
ments, see Sec. 5
For Mastercycler gradient 5331, Mastercycler personal 5332,
Mastercycler 5333, Eppendorf offers the Temperature Validating System 0055
000.298.
For Mastercycler 384 5334, Eppendorf offers the Temperature Sensor
5331 233.007 for Temperature Validating System 0055 000.298.
10.8 Noise level < 70 dB (A)
Remarks:......................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
Signature of customer ......................................... Date: ....................................................................

11. Maintenance protocol

Mastercycler 5333
Mastercycler 384
Serial number:
For Mastercycler 5331, see operating instructions 5331 900.062, Sec.11

For Mastercycler 384 5334, see operating instructions 5334 900.101, Sec.11
The device was operated according to existing lab safety regulations.
Remarks: .......................................................................................................................................................

dure
11.1 Disconnect mains plug.
11.2 Allow device (thermoblock and heated lid) to cool down to room temperature.
11.3 Clean the outside of the device with water and a mild cleaning solution using a
damp cloth.
11.4 Disengage the heated lid and clean the inner surface using a damp cloth.
11.5 Clean the sample tube position in the thermoblock using a moisted Q-Tip.
Remove broken test tubes if neccessary.
Remarks: .......................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
.....................................................................................................................................................................
Maintenance Repair
Date of next PQ:...................................................

Appendix
Date Service or change performed, copy of paperwork attached


Mastercycler 5331 - 5334 Service Manual English

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Mastercycler 5331 – 5334

PACT information sheets 2

Error lists / Troubleshooting 3

Spare parts list 7

(blank) EDP | Printer 12

2 PACT information sheets

3 Error lists / Troubleshooting

Mastercycler 5331 / 5333 / 5334........................................................................................ 4-5

KS/1109 Contents 0-1

Mastercycler 5331 – 5334................................................................................................... 5-6

Checking the sensor functions ......................................................................................... 5-7

7 Spare parts list

0-2 Contents KS/1109

12 EDP / Printer (blank)

KS/ 1109 Contents 0-3

0-4 Contents KS/0603

Please read carefully before servicing the device!

• All servicing is performed at the technician’s own risk!

MSTM/MMWT/ 1301 Safety precautions 1-1

1-2 Safety precautions MSTM/MMWT/ 1301

MSTM/MMWT/ 1301 PACT information sheets 2-1

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New service spare parts for Mastercycler 5331 / 5332 / 5333

For Mastercycler 5331/5332/5333 RAM module 0013 591.458

5332 810.008 5331 013.007

F1 0.5A, slow 0013 606.072

1999-07-23 / MMT / U.Krohss 53319T01.doc 1 (1)

Garantieregelung für Mastercycler 5331 / 5332 / 5333 / 5334

Da es immer wieder Anfragen zu Garantiezeiträumen bzw. unvollständige Angaben bei

1. Seriennummer des Gerätes, siehe Geräterückseite.

Nur mit diesen Angaben kann eine zügige Reklamationsbearbeitung erfolgen.

Wir hoffen, hiermit alle offenen Fragen geklärt zu haben.

Warranty procedure for Mastercycler 5331 / 5332 / 5333 / 5334

All the above-mentioned devices are covered by a warranty period of 24 months.

We hope that we have herewith resolved all pending questions.

2001-08-03 / / 53311T02.doc 1 (1)

New Software V 2.30 for Mastercycler 5331, 5333 and 5334.

This new software is valid for all mastercyclers with flashloader.

The software update disk 5331 835.007 is no longer available.

Computer PCBs without flashloader can only be updated by replacing EPROMs.

Current software update as EPROM versions:

Temperature sensor for 384-well thermoblock 5331 233.007 (new)

These PCBs have been modified to eliminate the „Power Error“.

2002-01-29 / / PACT53312T01.doc 2 (2)

New temperature validation system

2005-05-26 / MST / UK Mastercycler_ep5T02 1 (1)

2-2 PACT information sheets MSTM/MMWT/ 1301

Error Cause Solution

BLOCK ChkSumErr Data error. Store calibration data and

BLOCK DEFECTIVE Defective electronics, Note:

MSTM/MMWT/1301 Error lists/Troubleshooting 3-1

Error Cause Solution

EEProm ERR! EEPROM not found. Defective sub-assembly

3-2 Error lists/Troubleshooting MSTM/MMWT/1301

Error Cause Solution

Err Invalid Invalid command. Enter command completely or

MSTM/MMWT/1301 Error lists/Troubleshooting 3-3

7KHKHDWHGOLGIRUJUDGLHQW /LGVHJPHQWQR 6WHPS

7KHKHDWHGOLGIRUSHUVRQDO /LGVHJPHQWQR 6WHPS

DQG/LGVHJPHQWQR 6WHPS

6RPHRUGHUQXPEHUVDUHQHZ 1HZSDUW 3RROSDUW

7KHUPREORFNSHUVRQDO

7KHUPREORFNJUDGLHQW

3&%SRZHUXQLWJUDGLHQW DQGDUHFDQFHOHG