ACETILCOLINA 10-2

Concentración Concentración
Volumen (mL) Log C GMS Promedio GMS %E Probabilidad
(M) (mg/mL)
0.19541
1 1E-09 1E-07 -7E+00 0.14656 0.2084
0.28334 1.61 0.016
10.806
1 1E-08 1E-06 -6E+00 10.982 2.5729
10.923 19.86 0.199
14.421
1 1E-07 1E-05 -5E+00 14.069 9.3828
14.187 72.43 0.724
14.597
1 1E-06 1E-04 -4E+00 14.431 12.3106
14.45 95.03 0.950
14.568
1 1E-05 1E-03 -3E+00 14.421 12.6960
14.753 98.00 0.980
14.577
1 1E-04 1E-02 -2E+00 14.646 12.9555
14.871 100.00 1.000
14.45
1 1E-03 1E-01 -8E-01 14.558 12.7670
14.704 98.55 0.985
15.007
1 1E-02 1E+00 2E-01 14.89 12.4700
14.707 96.26 0.963
14.91
1 1E-01 1E+01 1E+00 14.831 7.6697
14.48 59.20 0.592
0.5862
0.1 1E-10 1E-08 -8E+00 0.4201 0.4722
0.4103 3.64 0.036
0.2403
0.01 1E-11 1E-09 -9E+00 0.2051 0.1973
0.1465 1.52 0.015
0.07816
0.02 2E-11 3E-09 -9E+00 0.127 0.0814
0.039 0.63 0.006

ACETILCOLINA 10-4
Concentración Concentración
Volumen (mL) Log C GMS Promedio GMS %E Probabilidad
(M) (mg/mL)
3.3512
1 1E-09 1E-07 -7E+00 3.234 3.1363

2.8236 21.30 0.213

 10.552
1 1E-08 1E-06 -6E+00 10.777 10.6987

10.767 72.65 0.726

14.314
1 1E-07 1E-05 -5E+00 14.226 14.2063

14.079 96.46 0.965

14.235
1 1E-06 1E-04 -4E+00 14.48 14.3330

14.284 97.32 0.973

14.831
1 1E-05 1E-03 -3E+00 14.763 14.6520

14.362 99.49 0.995

14.841
1 1E-04 1E-02 -2E+00 14.7631 14.7270

14.577 100.00 1.000

14.538
1 1E-03 1E-01 -8E-01 14.792 14.5837

14.421 99.03 0.990

14.753
1 1E-02 1E+00 2E-01 14.45 14.6227

14.665 99.29 0.993

0.2831
0.1 1E-10 1E-08 -8E+00 0.4982 0.4005
0.4201 2.72 0.027
0.3126
0.01 1E-11 1E-09 -9E+00 0.1367 0.1986
0.1465 1.35 0.013
0.3517
0.02 2E-11 3E-09 -9E+00 0.127 0.1791
0.0586 1.22 0.012

ACETILCOLINA 10-6
Concentración
Volumen (mL) Concentración Log C GMS Promedio GMS %E Probabilidad
(mg/mL)
1 1E-09 1.4621E-07 -6.83502292 3.3024 3.1298 21.5714384 0.22
3.1168
2.9702
1 0.00000001 1.4621E-06 -5.83502292 10.425 10.6173333 73.18 0.7318
10.777
10.65
1 0.0000001 1.4621E-05 -4.83502292 14.06 14.037 96.75 0.9675
14.04
 14.011
1 0.000001 0.00014621 -3.83502292 14.548 14.3623333 98.99 0.9899
14.05
14.489
1 0.00001 0.0014621 -2.83502292 14.148 14.3953333 99.22 0.9922
14.529
14.509
14.587
1 0.0001 0.014621 -1.83502292 14.421 14.509 100.00 0.999
14.519
14.265
1 0.001 0.14621 -0.83502292 14.177 14.3266667 98.74 0.9874
14.538
14.626
1 0.01 1.4621 0.16497708 14.226 14.47 99.73 0.9974
14.558
0.6057
1.4621E-08 -7.83502292 0.5276 0.47221333
0.1 1.00E-10 0.28334 3.25462357 0.03254624
0.33209
1.4621E-09 -8.83502292 0.29311 0.23771
0.01 1E-11 0.08793 1.6383624 0.01638362
0.1661
1.4621E-10 -9.83502292 -0.039082 0.06189267
0.02 1E-12 0.05866 0.4265812 0.00426581

12.9555
 UP M = no. Mol/ Acetilcolina 146.21 g/mol
1 g = 1000 mg
1E-09 mol - Xg 1 L = 1000 mL
4.190 1 mol - 146.21

X .= 1.4621E-07 g/L
5.610 X .= 1.4621E-07 mg/mL

6.640 Gráfi co 1. Respuesta de aceti lcol

Promedio GM%E LogC vs %E
12.9550 100
120.00
6.960 X
100.00

80.00
7.070
60.00
%E
40.00
7.290
20.00

0.00
7.050 -2E+01 -1E+01 -5E+00 0E+00
Log C

8.090 Acetilcolina 10-2 Acetilcolina 10-4 Acetilcol

7.370

Gráfi co 2. Aceti lcolina. Log C vs Up

3.120 10.000
8.000
6.000
Up

4.000
2.670 2.000
0.000
-2E+01 -1E+01 -5E+00 0E+00 5E+00
Logc

Acetilcolina 10-2 Acetilcolino 10-4 Acetilcolina 10-6

UP M = no. Mol/ Acetilcolina 146.21 g/mol

1E-09 mol - Xg
4.19 1 mol - 146.21
 X .= 1.4621E-07
5.58 X .= 1.4621E-07

6.75
Promedio GM%E
14.7270 100
6.93 X

7.58

8.09

7.33

7.46

2.95

2.67

2.67

UP

4.23

M = no. Mol/ Pilocarpina 146.21g/mol

5.61 1 g = 1000 mg
1E-09 mol - Xg 1 L = 1000 mL
1 mol - 146.21
6.75
X .= 1.4621E-07 g/L
 X .= 1.4621E-07 mg/mL
7.29 %E
Promedio GM%E
14.509 100
7.41
X %

8.09

7.23

7.75

3.12

2.67

95.0221913
 puesta de aceti lcolina.
LogC vs %E
120.00

100.00

80.00

60.00

40.00

20.00

0.00
-5E+00 0E+00 5E+00
Log C

Acetilcolina 10-4 Acetilcolina 10-6

 ACETILCOLINA + ATROPINA (1E-2)
Concentración Concentración
Volumen (mL) Log C GMS Promedio GMS %E Probabilidad
(M) (mg/mL)
0.1074
1 1E-09 1E-07 -7E+00 0.1954 0.1302 0.91 0.01
0.0879
0.5086
1 1E-08 1E-06 -6E+00 0.2247 0.2998 2.10 0.02
0.1661
0.0488
1 1E-07 1E-05 -5E+00 0.2247 0.1302 0.91 0.01
0.1172
0.3224
1 1E-06 1E-04 -4E+00 0.1074 0.1726 1.21 0.01
0.0879
0.3028
1 1E-05 1E-03 -3E+00 0.2833 0.2344 1.65 0.02
0.1172
0.3712
1 1E-04 1E-02 -2E+00 0.4494 0.4657 3.27 0.03
0.5764
3.1851
1 1E-03 1E-01 -8E-01 3.2024 3.2658 22.93 0.23
3.4099
11.168
1 1E-02 1E+00 2E-01 11.021 10.8870 76.43 0.76
10.472
14.411
1 1E-01 1E+01 1E+00 14.03 14.2450 100.00 1.00
14.294
0.3615
0.1 1E-10 1E-08 -8E+00 0.39 0.3189 2.24 0.02
0.2051
0.3126
0.01 1E-11 1E-09 -9E+00 0.1954 0.2019 1.42 0.01
0.0977
0.3224
0.02 2E-11 3E-09 -9E+00 0.1856 0.2019 1.42 0.01
0.0977

ACETILCOLINA + ATROPINA (1E-4)

Concentración Concentración
Volumen (mL) Log C GMS Promedio GMS %E Probabilidad
(M) (mg/mL)
0.3224
1 1E-09 1E-07 -7E+00 0.0879 0.1791 1.22 0.01
 1 1E-09 1E-07 -7E+00 0.1791 1.22 0.01

0.127
0.2735
1 1E-08 1E-06 -6E+00 0.2051 0.1725 1.18 0.01

0.039
0.2247
1 1E-07 1E-05 -5E+00 0.127 0.2067 1.41 0.01

0.2683
0.6937
1 1E-06 1E-04 -4E+00 0.6546 0.6253 4.27 0.04

0.5276
3.2242
1 1E-05 1E-03 -3E+00 3.0777 3.1526 21.51 0.22

3.1558
10.532
1 1E-04 1E-02 -2E+00 10.298 10.4770 71.47 0.71

10.601
14.275
1 1E-03 1E-01 -8E-01 13.972 14.1347 96.43 0.96

14.157
14.929
1 1E-02 1E+00 2E-01 14.421 14.6587 100.00 1.00

14.626
0.3615
1E-06 1E-08 -8E+00 0.39 0.3189 2.18 0.02
0.2051
0.2931
0.1 1E-07 1E-09 -9E+00 0.254 0.1986 1.36 0.01
0.0488
0.0781
0.01 2E-07 3E-09 -9E+00 0.1172 0.0683 0.47 0.00
0.0097

0.02

Concentración
Concentración Log C GMS Promedio GMS %E Probabilidad
(mg/mL)
0.04885
ACETILCOLINA + ATROPINA (1.0E-6) 0.32242 0.22797333
Volumen (mL)
1E-09 1.4621E-07 -6.835022923 0.31265 1.5977946 0.01597795
0.43967
0.71324 0.60576667
1 0.00000001 1.4621E-06 -5.835022923 4.24563125 0.04245631
 0.60576667
1 0.00000001 1.4621E-06 -5.835022923 0.66439 4.24563125 0.04245631
3.234
3.3708 3.31866667
1 0.0000001 1.4621E-05 -4.835022923 3.3512 23.2595085 0.23259508
10.855
10.777 10.7963333
1 0.000001 0.00014621 -3.835022923 10.757 75.6681619 0.75668162
13.747
14.04 13.8316667
1 0.00001 0.0014621 -2.835022923 13.708 96.9418746 0.96941875
14.509
13.913 14.2093333
1 0.0001 0.014621 -1.835022923 14.206 99.5888235 0.99588823
14.587
14.128 14.2353333
1 0.001 0.14621 -0.835022923 13.991 99.7710494 0.99771049
14.314
14.255 14.268
1 0.01 1.4621 0.164977077 14.235 100 1
0.39082
0.0977 0.25728667
1 1E-10 1.4621E-08 -7.835022923 0.28334 1.80324269 0.01803243
-0.06839
-0.00977 0
0.1 1E-11 1.4621E-09 -8.835022923 0.07816 0 0
0.19541
0.29311 0.22146333
0.01 1E-12 1.4621E-10 -9.835022923 0.17587 1.55216802 0.01552168

0.02
 UP
Promedio GM%E
14.2450 100
X
2.670

2.950
Gráfi ca 3. Aceti lcolina + Atropina. Log C vs %E
120.00
2.670 100.00

80.00

60.00
%E

2.670
40.00

20.00
2.950 0.00
-2E+01 -1E+01 -5E+00 0E+00
Log C

3.120
Acetilcolina + Atropina 10-2 Acetilcolina + Atropina 10-4
Acetilcolina + Atropina 10-6 Acetilcolina

4.260

5.710

8.090
Gráfi co 4. Respuesta de Aceti lcolina + Atropina.
Log C vs Up 9.000
8.000
7.000
2.950 6.000
5.000
Up

4.000
3.000
2.670 2.000
1.000
0.000
-2E+01 -1E+01 -5E+00 0E+00 5E+00
2.670 Log C
Acetilcolina + Atropina 10-2 Acetilcolina + Atropina 10-4 Acetilcolina + Atropina 10-6

UP
Promedio GM%E
14.6587 100
2.67 X
 2.67

2.67

2.67

3.25

4.23

5.55

6.75

8.09

2.9500

2.6700

2.6700

UP

2.67

Promedio GM%E
3.25
3.25 14.268 100
X

3.87

5.67

6.75

7.58

7.75

8.09

2.67

2.67

2.67
pina. Log C vs %E
120.00

100.00

80.00

60.00

40.00

20.00

0.00
0E+00 5E+00

olina + Atropina 10-4

olina

Atropina.

00 5E+00

etilcolina + Atropina 10-6

 Acetilcolina
Log Con U.P.
1 -9 2.67
2 -8 4.12
3 -7.301 5.23
4 -7 5.58 Gráfico 1. Respuesta de a
5 -6 6.64
6 -5 6.88
7 -4 6.55
8 -3 7.17
9 -2 7.12

Unidades Probit (UP)

Acetilcolina + Atropina
1E-2
Log Con U.P.
1 -9 2.67
2 -8 2.67
3 -7 2.67
4 -6 2.67
5 -5 2.95 -9.5 -9 -8.5 -8 -7.5 -7 -6.5 -6 -5.5

6 -4 4.19 Log Concentracion

7 -3 5.39
8 -2 6.48

Acetilcolina + Atropina Acetilcolina + Atropina

1E-4 1E-6
Log Con U.P. Log Con U.P.
1 -9 2.67 1 -9 2.67
2 -8 2.67 2 -8 2.67
3 -7 2.67 3 -7 2.95
4 -6 3.25 4 -6 4.08
5 -5 4.33 5 -5 5.58
6 -4 5.1 6 -4 6.64
7 -3 6.8 7 -3 7.12
8 -2 6.48 8 -2 6.48
fico 1. Respuesta de acetilcolina. LogC vs UP
8

0
8 -7.5 -7 -6.5 -6 -5.5 -5 -4.5 -4 -3.5 -3 -2.5 -2 -1.5

Log Concentracion Acetilcolina

Acetilcolina + Atropina 1E-2
Acetilcolina + Atropina 1E-4
Acetilcolina + Atropina 1E-6