Flor Cecilia Caraveo Cola ID: 212a2q 22 loz | 2022
Asignacion B= Adliculo: Extiaccion de cokigeno a
porliv de preles de tilapia
4- \dentificai la Metodolog fea
Este estodio realize ona ene de colecjeno a parliv de lo piel
cle Hapia. Raa elo se domaron muesticg de Piel de Tilapia la cual
Se verificd que compliera con les eslindares de calidad edolblecices
Raperiencics, clor, 1élura). Despues s¢ hicieron tecortes de piel de lem
Xoo. Posteriomentle se decolorervon las pieles con Npoclerito de so
dW al aszs-% (plv) dorente 30 minvlos a tempeiatora ambiente en
Meleci&a 1:10 ply con lea Murdo de piel. Sequido a exo se reulizun
Hes lewados Con cque devtilede puter cherry, por completo el hi-
feclorilo de sodio. Beyoués se vealizd una Nididlists enzimedicer pos
climinar poteinas distinla, al coldgeno. Posterior a a0, s¢ hizo anes
Sobolizactén de colégeno en medio deido PEMA Sepeuet por mao
el coldgeno de la piel. Se Mer c cabo one Filliacian del colkageno
en exlado Mquido, despues este xe precipild con clorore de soaio
a '0% po Vhows a temperatura ambiente, Pores tecoperor el cole-
Gene sblido, se FIO y Xe lb thes vere con agua devilide peucs
eliminer impuezas. For Ultimo el coldgeno + quaido en fiascos de
cristal edériles en eehigetacioa a 4 °C.
2>Diagiama de Flojo de proceso de hidglisis
Anadiv reciclivo
SIGMA PRINI-SOm| al 3%
Aateger Scloctin buffer
Trig -HCl_ en pH 8.6
[Reporor 7:70 hoes 0 18°C |
wor tres vecey con aqua dalilede
Escaneado con CamScanner Ar ldentificcn el metodo quimico © biolegico. =
E| mélode de exitacioa de! colageno y < dr ‘tuonhficucion de
profeinas Variables a comboler.
*Temperaluia : 7B- 40°C pew lu etraccion del colkigeno; 85°C
crt la hidiglisis enzimddicn
"eH: 3,864 4
‘Tiempo: 18,30 0 60 minctoy pau 4 blengueemiento ; 2-3
heros pura la hidedlisis -
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