Title:

RF

• Temperature: 18-25 ْ
• Specimen number:
• Introduction :
Huma Tex RF is a latex agglutination test for the qualitative and semi-quantitative
determination of Rheumatoid factors (RF). in human serum.
The assay is intended for professional use and should be used by trained personnel only.

• Principal:
HumaTex RF is based upon the agglutination reaction bet ween Rheumatoid factors (RF) of a
patient specimen or control serum and human immunoglobulin G (IgG) coated onto polystyrene
latex particles. The positive reaction is indicated by distinctly visible agglutination of the
latex particles in the test cell of the slide.

• Kit content:
LR 40 or 100 RF latex reagent (white cap)
- Suspension of white polystyrene latex particles , coated with human immunoglobulin G (IgG)
1.0%
PC 1.0 ml Control Serum Positive (red cap)
-Ready for use sheep serum control, producing a distinct agglutination Anti-human IgG (goat)
NC 1.0 ml Control Serum Negative (green cap)
-Ready for use control, non-reactive with LR 1 Slide with 6 cells
REF 40037:
GBS 100 ml Glycine-NaCI Buffer pH 8.2 -+ 0.2
Glycine 100 mmol/l
NaCl 1g/l

• Kit information:
-company name: Human Gesellschaft für Biochemica und Diagnostica mbH
-expiration : 2017-8
-lot number: 16002

• Dilution: doesn’t need to do the dilution because the result is negative.

• Results:negative for (RF)
• Discussion:
The clinical signi cance of RF determinations consists in differentiating bet ween rheumatoid
arthritis, in which the rheumatoid factor has been demonstrated in the serum of
approximately 80% of the cases examined, and rheumatic fever, in which the rheumatoid
factor is almost always absent.
The RF test is more frequently positive in long term active processes than in diseases which
are less active or are still in early stages. RF are occasionally found in the serum of patients
with polyarthritis nodosa, systemic lupus erythematosus, hepatitis and certain other
diseases.


• Procedure:

Qualitative determination

Bring LR ,PC,NC and serum samples to room temperature.

Mix LR carefully prior to use to suspend the latex particles

completely.

Pipette / drop onto separate cells of the slide:

Serum sample 40 ul

PC, red cap 1 drop

NC, green cap 1 drop

LR, white cap, onto all sample and control cells.

1 drop each

Mix with separate sticks and spread the uid over the

entire area of the particular cell.

Tilt the slide back and forth for 2 minutes so that the

mixture rotates slowly inside the cells or place the slide on

an automated rotator at 100 r.p.m..

At the end of the 2 minutes read the results under bright arti cial
light.

• Procedure:

Semi- quantitative test