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ELISA (enzyme-linked immunosorbent assays) originally described by Engvall and Perlmann (1971) have
been the mainstay of detection of a wide variety of proteins, including cytokines (see Stenken and
Pschenrieder, 2015), lymphokines, phosphoproteins, and many recombinant molecules
doi: 10.1002/0471142735.im0201s110