Product Information GSure® Viral RNA Isolation Kit from Blood Avaiz ack size Catto, ck sie a covRtores: s0Prep a ‘Storage: Trizin Reagent slore at 4°C & dark and rest store at room temperature, Procedure: 4, Sample lysis and phase separation NB. Ifthe blood sample containing nucleated RBC as well a8 volume less than 200, add the appropriate volume of PBS to maintain the volume 200 + Inbal at rom temperate fe 5 minutes. and cone the ste for § miles at 12000 » jl 10°, ten arse the clear supernatant 8 new tube + Incubate fr 2:3 mutes at com eerie ‘Fert mL TRIN, ed 2004 chefom, secur he cap tat and vere or 18 sands. “+ Inebate or 23 mines at oom terperaue. + Cantuge a 12.00 xg for5 ints al 4. ‘+The mitre separates nin 2 ler pink prenckchlorfom phase, an interphase and an upper cours aqueous pase NB. All subsequent contrfugation steps should be performed at room temperature 2. Pre-purification of blood cell lysate "> The colouriess upper aqueous phase & carefully aspirated. + careshoue tte prevert dtuticg te nepase stove For i TRITA Reape: ose. $00 plo aguas huss ope be reeves wih ose Q “eau pe tes ah A SO conn a 20x 30 sects. + Colette ow-tough nates miooige ube long of RHA to membrane or in TRIN used, 2060 of noproparol othe elected owtrough, 2 txt yen SLced-60 oko RBC OOPS NK oun). NNB.Donatoed more than 700 po the column, « «+ Canrige at 120090 sexs aan emprtre. {ad ow tanh sete RNA Bn can i he sae cocton en Lot teeing soln nd eae at 1200950 soda ‘com aro + Dec for set he RNA Beg ol oe sane len te Washing of RNA + Ra lot menivare wah fe? oo he RNA Br coum (Be) [NB Dilute with absolute ethanol (Molecular Bology Grade) as indicated before use Cerra 12000 xg 50 stots trom pera Discarded 60D pf marae wash buf no be RNA Big clue + Gente a 12000x or 30 sears atom tempers ad fo co Cente a 1200 or nus aroom rare ot of ny real wash be 'NB. This step is extremely important for optimal downstream performance ofthe puted RNA lution of RNA ‘sz te calcio tbe and place fe RNA nrg slum Naseem icra be ‘+ Ad! 80 plot provided nuclease fee water athe cen of he RNA inn clam Lette fort mint. Centrivge at £2000 x gor? mints at oom temperate Dissardthe cre column, Te Sl mile tbe cata be purifles oel RNA, ‘Ste paified RNA on eo be used win afew hours, Fr long. term storage, soe the pred ANA at-60°C + highly gue RNA without genomic DNA contanaton required, ‘pero DNase | veanent afr puricston, aly te quay and aunty fhe obted RNA Kit Content soPrep Gm Membrane Wash Bute om Pre uifaon Couns White) 50 8 RNA Bring Columns (ve) 50 pos Nuclease sae Wale Smt Additior lute ethanol into provided Membrane We uffer ] Absohte EORTobe See ad for 5 prep —Serbrane Hath But 45 Note: ‘Reconstitution of Membrane Wash Buffer: Before sr he it ofa tee, ad abso ear (lool tog grace) wih ‘he provid bar, Mx hacugly y shaking. Once ehancl ade, ben te cap ropa afer each use For Technical Support Contact : oychandipur, Bakrahet, 24pgs.(S),PIN-743377, (WB), INDIA Emeil: tech.support@cccbiotech.co.in Ph: 033-24951044 / 24950004, www.gccbiotech.co.i @ccc\\Biotecw’