GENERAL LABORATORY INSTRUCTIONS

1. Undertake no unauthorized experiments.

2. Wear safety glasses.

3. Wear shoes that fully cover the feet; sandals are inadequate.

4. Wear protective clothing; lab aprons or lab coats but old clothes are satisfactory.

5. Should know the location of following equipments and how to operate them.

(a) Fire extinguisher

(b) Safety shower

(c) Eye fountain or spray

6. Observe the following simple laboratory practices.

(a) Never taste a chemical.

(b) Never pipet a liquid by mouth; always use a rubber bulb.

(c) Never smell the vapors of a chemical except by gently fanning some of the vapors
toward your face; do not breathe deeply.

(d) Never generate poisonous or strongly irritating gases except under a hood that has
strong ventilation.

(e) Never pour water into a concentrated acid; always slowly pour the acid into the water
with stirring.

(f) Never heat the materials in a test tube unless the mouth of the test tube is pointed away
from both you and your neighbors; even so, heat gently on the side rather than the bottom.

(g) Never insert glass tubing into a cork or rubber stopper unless the end of the tube is
fire-polished, the glass and rubber are lubricated with water or glycerin, and your hands
are protected by toweling and held away from the end of the hole through which the tube will
emerge.
 DNA isolation stocks (100 ml)

SI Components Stock Concentration/ Volume Working concentration

No
.
1 Extraction buffer 100 mM Tris HCl, pH 8.0
(CTAB) 20 mM EDTA, pH 8.0
1.4 M NaCl
2 Potassium acetate
3 Sodium acetate
4 PVP (write fullform)
5 T10E1
6 RNase
7 Ethanol

Gel electrophoresis components (100 ml)

SI Components Concentration Amount to be

No. added
1 TAE buffer
a. Tris base
b. Glacial acetic acid
c. EDTA
2 Agarose
3 Loading dye
a. Bromophenol blue
b. Xylene (optional)
c. Glycerol
4 EtBr
5 100 bp ladder
Water: dye: ladder