Assay of Ascorbic Acid

Analyte – 200mg Triturate Ascorbic acid

Titrant – Iodine
Indicator – Starch TS
Endpoint – Yellow à Deep blue/ Bluish Black

Initial volume – 25mL

Final volume – 17.5mL
Final volume reading – 7.5mL

mL ( Titrant ) x N ( Titrant ) x mEq( analyte)

Assay%= x 100
weight∨volume of Sx .

Molecular weight
mEq =
Factor x 1000

7.5 ml x 0.1 x 0.088

Assay%= x 100
0.2 g

Assay%= 33% USP: 99.5-100.5%

176 g /mol
mEq = = 0.088
2 x 1000