i alent Nombre del Alomno + Anette Jac! Diaz Gonrol Grupo 43. IG (QFE) cos NutveqencdaS oe: 7 Biogpimjco meto! Seal oko i Proey BASES NVTROGENADRS and RNA Choose the correct option (s) and Quiz: structure, function and metabolism DNA Justify your answer MELE oaptora eo la ARN eins 1. The transcribing enzyme is:= polymerase te Se oe ‘franscriggdén , \a ARN los prante ot Ov PRionTeR hose ena copia de un gen det AEN ot ARM TITER imerasa 3. Necesario. Dorante la tremscripciéa, el sitio det AON end ge be une la ARN denomina promoter: La transcripcién comienta. Cuan uno. & mds eroteinas del Faces ciqmn ¢. onén a la Folotnima de. ARN golimerasa ; Lo qele peenite, Unirse al ADN promotor. La ARN golimerasa Crea. Ono borlaja de transcigsify Qet S6para las cles helras de ta. heltoe del AON . Esto de hate rompiendo les enlances de hidrdgtno entre nudeétidos de AON. Complementares. 2. Which of the following statements is true regarding introns?) ‘A. Introns are the parts of mRNA that are translated. B. Introns have no function. In general. human genes have fewer introns than genes of other organisms. Exon8S POD D. Introns may be involved in exon shuffling éste Im portant descp bd miento oF et GAlbcct [66 Gi6 toga JErornaS Putran| mis Aomplejes {uersificades, . Lalbaraja de Ie: ds kavdlde elidenintel de iran ratclida (responsable fi fora £4 Adéinds , lob intvores pudden intervompir el morce dey lect insérdads Q gum Secuencia entre dob dodenes condecohuas! (tv ©) entre el primer 4 Sekyndo hodedhde (intvones de Fosd 4) 6 equdo u dereer nockefhida de, tn Codth (intrones dd Fase |2)- ja nt | | 3. Which of the following statements is true regarding transposons? A. They are sequences of mRNA that can move around in the genome. B. They exist in corn, but are not found in the human genome. C. They are the most abundant type of repeat in the genome.” ab D, All of the above are true regarding transposons. i ONL ciby Gené Keg gera. emnoueese, dentro de lo: " A S Bergmos Las Secvendas Pep colonel ok 26 al a5 7% dha tenrendo la chase mobs abundante des secwenda rop hloteando de éstes tna Be coead, Korean Cromebo! aly eae wal vio desea goes 8 alee mma ee a Gorm 6h genera rene aa4. The niteo edt from all of the following ogen atoms of a purine molecule are devin all of the nine aelds EXCE una malhwla ot & i ro de one tr oe ferns Los Gtomes de niteeae oe BRON oe nctcliies excepve Sern ee caiman & te Sahsa Greteioa 2 Micenhes qe los purinas BON ere: ve teen (ond Siren Ar Aselel ailts wir eho eles exes : Cae tebe bata peyton Se Sales Wate serene oak ee eS ges i eesares en ln i athe #8 | Lis PLAnS Sch Neo chmpintirng Eretosss emai Me betty deieretar dell caerieey & trum Pep L® Orca. $- In tissues that do not carey out sete de novo nyntie maintenance of 19 —=———$—$——— adequate i ‘ i realiuan athte- . A peo NO. e salvage ving APRT Eo oi * NONO , raters eke A. occur’ ps elecaide phiosphorytare, Gn GOS deepen eh rescoke, usondo HE-PRT, G. D. is accomy complished entirely by the action of adenylate kinase. ee, involves hypoxanthine salvage using HG-PRT, eokoriice is eee neni a ceucas peerete de: poring , asf como la ee ra geese gelnaente: te sintestS . whi of the following is used during the conversion of uracil to thymine? To AML Gl THF CtetvahideoRirore) 3¢ um domante, Ia converter Sé-crabils Gi Gomer A timina pore ere: ARNEL Undlo x Urea la hae 6s Oe Seach D. Biotin mala sircete iad E. COs 110.C meetvada de. Lainie 4 me cL THE pore Quose pada The thajor control of de nove pyrimidine nucleotide synthesis in man is: D blood. . feedback inhibition of aspartate transcarbamylase, C. availability of N-acetyl glutamate substrate availability. unpetitive inhibition of carbamoyl phosphat ehtiva dé barbamoi ‘ ATP én oft. ne en +e set tg ae pletion | 8 ‘Thioredoxin is involved in the: La 4iorredo,ina esta A. conversion of AMP to ATP. conversion of JUMP to dTMP. €n la comersi€in de un ie oxidase ay a treatment for gout inhibition of xantt E, degradation of nucleoprotein. TereeseS Ce AS RRS Kaela castesna-——§ ae A es Be encuentran en la parte emelnagd, es eco eaotoee eligi isele ee en \a-inlnctded ‘co la COR mola On Por ETP ParQey PeQV CG ime O Ame. PRP, yt dela coroay EP Bho contol imprtante fa la eneaga GO la cama a ee Ul aseatkals jen tena 18, Mays o rote i all of the Following EXCER Te un papel en Jos siquentel: cx, Jeotide pool by a salvage mechanism, ta \a Com ery) Bry i UTP O CTO Povtre GARE act Amindo4 © al Oy ais Gblitiadd ¢5/ la. amide des lo ais Mrs Ler ech SSoartnte araoiy clin el at “ci 4 wt Daily de. puta, tet La melédolo Ab ablediterdte (jal mens ide, cc ower, Sh witeaen) Cosa a Farm gartp fel anil ge) oc amntcival. \hibitor of Nant xidase, Administration of allopurinol to a=—————— ormal HG-PR levels would be expected to lead to all of the El aloporinel es cn inhibidor de la dasa . Se espaana Go \n patient with gout a x rm hess of IMP Ran cnelon + sraceietnthetions SRT SE OC AON cs eee E- increased xanthine in the blood. Mere bet o tedojlo st excepto (op muds clevades oe We HG—PRT) el avmento de: xantin e, pore mieahas este dis Rigenantna consomird PROD esto cpabuste, conta sttuacan del Sindrome: de Lasch —Nghan - 20. GTP inhibits which of the following reactions in purine biosynthesis?: A.PRPP >PRNH2 1 yeacsifn d& IMP7 MP @n la biosintests de BAMP SOME inhibe al ert? parqe enta. requlade cor ee eee oF rehoalimentacth de les nuctestides oo one The, Gm Py Ae Ta néveng lad 6n ide Ente doh (a) antesty ac AMP cor Lome Be ihibe la sinteins der Gait: 21, Carbamoyl phosphate synthetase I (CPS TI) is: $$ A. Activated by PRPP Ua. corloa men ReS tenes Sintetasa it (CPS es inhibido i E. All of the above are correct oes oe Be cS ps0, clay | Forenadén de UPes Facto § la efsar 2 en cateltvo Las i grec earbamat | Sous | cito5ol (a. diferencia det TL, Qu Ancona 2s Las ent tocondvas) 5 actinda pcr ALP al formar 4h carloamett Foafaks quo) ts Caer to 65 stage ob aronts g scearele a Sone FORE Ao ty u \ a lj © Be dco lore tea - BE ee oeet “2, Nucleic Acid Structure Explain why the absorption of UV light by double. stranded DNA increases (he hyperchromic effect) when the DNA is denatured,merbown HS 4 nitrepers 4 te glokanaicn : nikréaane & ~alicina: 9 Se Oe eye | a = rede eS rag bade par THY ees y = To restes CT Tacs)? conennn© =e On netlersidg Pos te bat, 14. A auctouside phosphors tase’ , ‘ f escinde ps poclehst and avelounide Con Ua rede deci ome t There hb 1 ie respond for the cquilbeation sf nucleowids iphosphare TAR WEA) ab eet Fiona, bs ae ee hea 7 easy Pees Sed penedan Gab. Pe £2. En iui Xe deo Coo on wate: sey AAlpdcke LS gorge: pee A Se isthe SS bea dal mend rence ne a ele rks te iar Weaken ot bol Fretatoy lee nips Bes. Wider fester Ger] einhe’ 4 Pap ex egollitad 4 heoia|t te Ven a Pe Be. Paring 6 « taeda | Globe Tnatere e3 W tend pamas st desamips ¢ Twosiaat ration of —— 15. Methotrexate is an inhibitor of dihydrofolate reductase. Administ : Inhibidor de lo Wide = D f seeded ode sone ge sue \ U ne | la cones is some a 1g de Noe - os ie eke 6, oe ca dete eet gehart metilo durante la tans ferencia. Be eee jee ek ae den ota parte la sf wees Sef ees ee Sa cea ae erie ima el elt {olato 8 BiekEAteS ts fora = defen a para eb Ara ae le eer a | 16. Phosphoribosy! pyrophosphate is: A. Formed during a regulated step in purine synthesis B Formed by the action of phoyphoribosy! synthetase Set rowlefalde (fas fovea sleet —— methotrexate would inhibit: A. de novo synthesis of UMP. “tion of inorganic pyrophosphate phosphatase a Aakcsvt oe iets lob Koeledivelas |e, Ae lal atabesis. de) npc D. Hydrolyzed by the Seheale inpliccidd 6 2p B ren laa on ce log oft Pe) Nowe) porna pinmidina, 17. Major controls of de novo AMP synthesis include ‘ oe ee do la sinte iKelegea \ fi alos mee imbibici6n’ tates on inbidoces oe a \O) WEED crime renewing NOT wineu in wberenvertOncy Siroe v deoxyribose? = 9. Which ofthe flo ae ce ubiliaa en lo. concersiGn 40 FORO B. FADH2 desoxirriboeo pore todem los Doses se 3atetitag D. Thiored Ch foceea. de RCOBG y 70 URN sre lorett eee oon Apes conuer ie en lo Forma : E Ribonucteotie rectane 9 ronson en, 9° eee eet ae So FAs para | co: Gn| donde (a Hore @ hina ge, Bae yf inkercamioio, conuersion deh “VeeMcies ida bRdo- ne En eh céakoleal kag SS cP BL Whar oe ioe tat Grama Ha seas i 1 will be oxidized. D. the final product will have the same type of nitroge IE. hipoxanthine will be an intermediate Phaka €) HUHgeAo) Ae Lo qpdeAa lated od 1/68h lors ‘ Ferd, la Macon cel nibtaero Se Fens Ta Avorechipi oa ri€ads ON gran Gere & Enero eorgee le Tedkcibe de Ng pore Forwer Nis implica (a roptural det tiple evlare! agglar deh pee aa abrio’ FE Are a LL. The formation of dATP for DNA synthesis occurs primarily by: ——_, La formadbn de dROP Gan. sy-containing ring as CTP. A. de novo synthesis beginning with PRPP. B. salvaging adenine using A-PRT. la sintesiS de AON ocorre CC. salvaging adenine usi phosphorylase and dR 1-P. Orncipa’ por conardie n ore WOR en AAD LSAMdo E. converting dIMP to dAMP using 5.10-methylene THF. Rorredoxine Pore Cl mmrcitante. UN NUCleSSido ADP vesultante se convertirla en trifesfato difesfato quinasa- 1 expected to contribute to hyperuricemt xorhoa 12, Which of the following would NOT b (gout)? La inhibicibs ola ax A. Unusually high levels of PRPP. contri byye o la hi perofceriae el Grice conte, areal ‘ sobre la 3% C. Unusually high turnover of nucleic acids. Grico 66 lads enili\idad Oe D. High activity of adenosine deaminase enalquier cot dre o E. Deficiency of HG-PRT. en la comentad6iy de soa Gases oak Ke pecotidemia » PREP [suces, la abi Sal Reet Sindests: eo Fekroalimen kad gn |. Tato © came 9 Condon fade ne PRT eS Ln contumilor Bate ide, PREP Pet an sing 86 necbeeradOh é/las Inoctebhiscs oe Ona dee tub |condouna alin alta sinbesié do made de, Bade Brice - Un wirilol dex & Tal xandina oxi doa |r fas iniveles 36 Grita - synthesis of IMP include: Las Fuentes di “13. Direct sources of purine ring atoms in the de novo_ ee Faire nornens dt Aime, MS Aer 22 € ROH se. ats Ras ca tecabbsrrertycky ment meree Shed. -Sonaxide como « tate: los Iaopen in prokar yon s hastcally the new: de, BRN ‘ha. del RR seg Aras ral Roping be caditeodoy oe is th. » Forge: eh me ine AN rorecateraae EE sfhtent BS eet Pears Cronhlta de HON” de ‘ deans Srecess te Recent Portada del mensaje a 24, How does transcription begi A. It doesnt, transcriptic © Some dude bn, D. The non-t Present and ready for E. The RNA polymerase binds 1 a costing strand located doveatram a Dering LSS Gg de Gelet daha tian (a. oe Pea Guordo ket [Gntireh ees ARAL one coder Ann welde: d cemienta a dofalriar (a Lie ee, NADL Bin que ta | nFormadthh Gene ce Gel ei) ee eh Ula pera. 90 25. Nucleotide Structure Which positions in the purine ring of a purine nucleotide in DNA have the potential to form hydrogen bonds but are not involved in Watson-Crick base pairing? : = los nik 1 anillo NA) N-3, NF I oat eet Stk Soe el Gallo Pe race a ‘ " at Sin emborap, N~1 esta frvoloccado er eke de ed tate Crick con tna PMmidina , g nea esta ia eceds ef Sten Seesott ectheta y ken! una. capcuddad dey enlace cae lmitada Pero sélo el N-3 4 NF Exton appa elas para. formar enamel Widedgerc ade ‘car doles. | aera i | | ia 1 {. 1 T3 EE — 26. Invotve(s) reduction and cleavage of “tn —— > A. Catabolism of gumine EA catawol Sone radlo umeltoce tm rececctmn g BiCatanoiemoruaeil!) —eqcid del anllo que At eres rhrhey are. Otreh) eo Sanina 8 cna puna y Ch Cnkle de Corre te 1. A only an ieia torre dtd Gitte: Pomerat sale Sey ertinde 2. Bonty eh aniline OWraidiva 3. Both A and B 4. Neither A or B lope optte cid would be an intermediates i = c = FO i eecanine Ny ef eat@laalione & la gartra wi del veil ‘peer stabolinsm of Ue RA ecmedcang® Fel dcldia oreo, gorges iette es ier eaterredtaw en ler ambons de los plvratawos pony Garo Wo de. L0S calaboltemes, mecctonides 2B only 3 3 Both A and B cuntedarrnan AorBy 28. What is the enzyme required to convert IMP to AMP from de novo prin ——— = Lin enLima Mteebana erro cower tie LOA an AMP ‘Ke de la Ka. ce sintest3 de B. GMP synthetase a ross €& la Adenia socks ae. © Nucleoside monophosphate Kinases Si nbe fas. ELC! # D. Uracil synthetase ce o~ - R Beers | ee. Pya = ot : a ou hake eer ime 29. Which of the following are inhibitors of enzyme that reduces Ribonucleotides to Deoxyribonucleotides A ART? ehidrorivrea Son lcs nhibidoreside ta en2i ma recixe) \OS vileonodiec6tidcs a.desonr— AATP & hy Bidar & hydroxyurea) yribnolestides Io vlburede btide: C. dATP & methotrexate es una. ereine fea cata jo Forrrachn Se D. Methotrexate & hydroxyurea Qesoxi vn o partir de nlarodestides, E, PABA & Methotrexate 730, Which of the following are inhibitors of enzyme that reduces Ribonucleotides to | Deoxyribonucleotides La OPW te lhidvoxi ved Met chores, _A. ATP & hydroxyurea eALIMA GO vechod, les Roneweee gates I ponxtebd& \cG alles conce: C. dATP & methotrexate gS AAP inklon la vi Scho Ke docked, witAbes: D. Methotrexate & hydroxyurea Ja Widroxt Urea clisranue lo prechucelimn ce i eho dran de> Alaceacho ble _E. PABA & Methotrexate mikonuxicohdns en a4 ley Wapttcadtod ay Tal Re oe 31. Which ofthe following molecules below IS NOT a part of the synthesis of a puriné——— La licina NO Forma parte, dy la Sinteds dew anillo de, A, Aspartate pone B.CO: BA \oS |) C. Glutamine D. cag emonophsp PRIY =o UAE £PUnidine monophosphate (UNE 3 Rete timiting step of de nove purine synthesis $= > S°Phosphonboss! 1p (PREP) 10 5° Phosphoriasylamine: by” BARON ntbente bast prominin snide dojla eben tet, S°Phospl phate (PREP) 10 5! Phosphoribosylamine by Ribose La Stntemts ae, Phasphate pst Peerewe, os © Ribose §- phosphate to Phoribosy!- L-pyropsphate (PRPP) by Ribose phosphate fer ee Foe fer tbesit Pyrophospohokin Anplirofenkintey D. There is no rate limiting step for de-novo purine symilesis a &! fen on Wotan: Foo bos Giornale Gmidatrarnfeesa Ocrast 41 Griecer es Sard sinks ce LoS PunaS dete ae la veleddad con ta PARC eatenpiaten tae’ s f 34. The end product of pycimtidine synthesis is: — — . A. Adeosine §- monophosphate AMP). nitarnyry cde, \ad FES, cetoreoas BL eee Fira Seria Drosinted 1s oe B. Inosine S- monophosphate (IMP) Pinientdinnd eB el UTR C. Uridine $- monophosphate (UMP) 3S. To make GMP as the END PRODUCT from de novo synthesis, you would require of following except: Roya hacer que SMP gs 8 el Podocto Anal a. ot ATP lasiintesis de Nevo no recesita de Gmp gereac este @ este 8 el predacto FINAL Y Gur lo tanto mo <9 reoQerclo 36. Ribose-s-phosphaste comes from whieh pathway for de novo purine synthesis prourene de la ula ple hyn | A. de novo purine synthesis Lex vibebass — hostate B. Shou oe Re cintens de guna dey rove: WG Laden enhe, lS a-G~Feaberto yy A! —S- as * D. TCA Kreb's Cycle | E. Urea Cycle | 37, How many molecules are ATP are required for de novo purine synthesis: A. O ATP, energy comes from b-oxidation requieren at) 4 mold las ck, AIP Pow lo sMtests de, ee 80) ocopa ARTE de actvacan 4 B.1 ATP | C.2 ATP tra de no Parque, | D.3 ATP Gres SATO CovallNS renastenes de ly, ¥ntears - | 38. CTP synthase catalyse which reaction Tgigu LTP Stvesa eataltun \EPTat — A CTP Cen glotamina come Nis | AUDP to CTP with glutamine as the NH af cm i Hs Dor gy 3 UMP to CTP lutamine as the NHx Donor He al 319 eI . with glutamate as the NHj acceptor = = a=stow down de nove pu wi hy > , ES ’ "ABA A analogs (slfonamides) because t won ‘competitively inhibit bacterial synthesis” PS.) \ CoC eS (ben SP, ee B a Fe itato) c jpacia mhpetitvely inhibit bacterial symthesis of folic GE, Mer Ae heras A STEERS Ge PUINA des PAL EH ce a Stnleats inctelona de, delete Atos er ea aise son, Prbeichcos Enact edicom Sunken cae Sr AMM ieee mee ect do B= PAT aaneclort ce th didrapreredte 1 EO Ml Aas necesitan Gama sinteklar Folecto gen Glbraa > Potnas y Want ee of the following isn't required for pyrimidine synthesis — rane | Gt Geilo FEI co NO eS re CEsato lagmress Ht Folic TAMIGiOS Pate Th Geido a ey masiOn oy Folate gore lo Peeeeaas Glutamine 41. Which of the following statements about the nomenclature of nucleosides is not La citosiinn 32 UO, S tclo redo noche 68 ti Aa true? is a nucleoside made up of the | ee (a ake © coside wale up ci base adenine on Datta elaine ck aay eo: No 4own al La. nomrenc de de, noche bsiclos ta e up of th dt L-ribo Porqwe bal crtocina bene 4 S desonimbesa Y bau seer enlace, “les Aude &tcles, one-carbon transfers reactions in purine Hienidive enlma cen na gluwxdtre es 0: 42. Which of the following statements about and pyrimidine nucleotide synthesis are corre 4? Please select all that apply. 1 transfer in purine nucleotide synthesis. ye synthesis of TMP. ide symhesis. "¢) Thymidylate synthase uses FH, (tetrahydrofolate) for th 0 d) N°-methy! PHs is used for the ore cubes ae purine nucleoti Lad Pankheasas c Sladek eH tle bcalrdroftfeto) ¢ (Aincorora late lini Viorica oh Val sine) de Rodo at pers a 5 reir De: