1. What is singlet, doublet and triplet state ?

Elaborate the factors affecting fluorescence.

2. Explain the instrumentation and application of flame photometry.

3. What is the role of electronic transitions in U.V. spectroscopy ? Discuss its

application in pharmaceutical industry.

4. Describe the any two types of detectors used in Spectroscopic technique.

5. What is ion exchange chromatography ? How ion exchange process takes

place ? Explain.

6. What types of injector, pumps and detector systems are used in HPLC ?
Enlist its pharmaceutical importance.

7. Discuss the fundamental modes of vibration in polyatomic molecules.

Explain the types of solvents and sample handling techniques used in IR
spectroscopy.

8. How thin layer chromatography is different from paper chromatography ?

Compare the techniques along with their applications.

9. Explain the following :

(a) Gel chromatography
(b) Adsorption chromatography

10. Write short notes on applications of any one of

the following :
(a) Gas chromatography
(b) Electrophoresis

Section—B
(Long Answer Type Questions)
Note : Attempt any four questions in detail. Each question carries 10 marks.
(4×10=40)

1. Discuss the instrumentation of UV visible spectrophotometer including

sources of radiation, wavelength selectors, sample cells.
2. Discuss the single component and multi component analysis by of UV visible
spectroscopy.

3. Discuss the instrumentation of IR

spectrophotometer including Sources of radiation, wavelength selectors.

4. Explain the principle, interferences involved in Atomic absorption

spectroscopy.

5. Discuss the principle, instrumentation involved in Nephelo-turbidometry.

6. Explain the workflow of the instrumentation of Gas chromatography

technique in detail

7. Give the comparison between any two of the following :

(a) Electrophoresis and Gel Chromatography,
(b) HPLC and Column Chromatography

8. Discuss the applications of any one of the following :

(a) Ion exchange chromatography
(b) Gel chromatography
(c) Affinity chromatography