REACTION OF TRYPHTOPHAN

1 NINHYDRIN TEST:
To 5.0 ml of tryptophan solution A violet colouration was got. It is due to the formation of
added 5.0 ml of o.1 % Ninhydrin Ruhemann's purple by the amino
solution and heated for 1-2 acid containing α amino group.
minutes and allowed to cool.
 
2 HOPKIN'S COLE TEST:
To 1.0 ml of tryptophan solution, A violet colour ring got at the It is due to the condensation of
added 2.0 ml of Hopkins cole junction of two liquids. aldehyde with iodine group in the
reagent and mixed thoroughly presence of sulphuric acid.
added an excess of H2SO4 acid
along the slides of the test tube.
 
3 XANTHO PROTIC ACID TEST:
To 2.0 ml of tryptophan solution A yellow colour was noticed in the This is due to the nitration of
added an equal volume of acid solution which turns bright aromatic acid to form yellow
concentrated nitric acid boiled orange with alkaline. coloured nitro derivation on
and cooled and then added treating with nitric acid. The salts
sufficient amount of 40% sodium of this derivation are arrange in
hydroxide drops. colour.
 
4 VOISE NET-RHODE TEST:
Treated 1.0 ml with tryptophan A violet coloured ring was got at the It is due to the condensation of
solution with a pinch of junction of two liquids. aldehyde.
P-dimethyl amino benzaldehyde in
con sulphuric acid.
 
5 ALDEHYDE TEST:
To 1.0 ml of tryptophan solution A violet coloured ring was got at the It is due to the condensation of
added one drop of 0.1 n in 500 junction of two liquids. aldehyde group with indole group
formaline, one drop of 15 % in the presence of sulphuric acid.
mercuric sulphide in 10%
sulphuric acid and then 3-4 drops
of concentrated sulphuric acid are
added.
 
6 EHRLICH'S TEST:
Preparation of Ehrilich's Test
Para dimethyl amino benzaldehyde in concentrated hydrochloric acid.
Added 2.0 ml of ehrlich's reagent A red colourisation was got. It indicates the presence of
in 0.5 ml of tryptophan solution tryptophan.
and heated in direct flame for few
minutes.