Griffith

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Griffith's Hershey Chase -

Experiment Experiment
I t
Transformation Transduction
 DNA -

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RAINIERPREE
It was not known that DNA is the

material
genetic .

It was called as
transforming
Principle because -

The material from

•

genetic
could transform another
one
organism material
organism receiving the genetic .
Griffith 's Experiment :

Streptococcus pneumoniae
L S

R strain
-
5- strain

Non virulent
-
Virulent
(Does not cause (causes disease)
disease)
T T
Hasa CAPSULE
No capsule lmuco polysaccharide)
T
Gives a shiny
appearance
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.

Smooth
strain

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**¥¥¥i¥j;Mh¥**¥¥¥¥ .

Rough
strain
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pm
l
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1
Smooth strain
capsule

f-vfh.mil
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•
•

•
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u Amman

WBC
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Rough No
Capsule
strain

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00

Ammu

WBC
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.
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Smooth strain
,,
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pm
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Rough
Strain

LIVED
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to 1*1
④
•

l l l r

LIVED
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B is

1*4
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f* ,•
,

DIED
something was transferred from heat
killed S strain to live R strain .

It converted R strain to
s strain
 Alfred Martha

Hershey f Chase Exp .

1952

Is hereditary material

DNA ? PROTEIN ? OTHER?

888¥
 Knows how

✓
to make new
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(⇐'*⇐
virus

q .ae#F-E-.*i.HYHHiF*
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qq.qig-EE-q.IE#---
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IN qq.qIEET-q.a.EE
qgq ÷¥
'T *Yg¥-g

What was the INJECTED

MATERIAL ?
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%t¥%qzr§g••µg*AIu¥
µq;☒gE☒ tPhha
i%eaqwmai.FI#g.
FAIRER .

Gg Gg →
Proteins are made of

polypeptides linked with

Disulphide bonds

→
DNA is made of

Phosphate groups
 INSIDE ?

0
°
0
①
k-Aoaw.s:*i:7 OR
+ bacteria
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OUTSIDE ?

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tphogy.ly
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INSIDE ?

qq.gr#q.+obacteria OR

OUTSIDE ?
0
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.

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,

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→±;,µÉ§%BEoa←→→
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it or
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fq e d a . M B A E . A # q R E -
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T­
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i@okamoai.E+.i g.Obacteria i@oqg.8o.aiE.ag 000
.

bacteria +
HYpÑFa O O O Ñ%ÑF* 000

Protein labelled DNA labelled

surfactant §o%a*m↳a±E÷* §oñawa*aE÷*

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First-hand
in qoqgao.a.q.iq ⇐•%kw•aE÷* ⇐•%kw•aE÷*
NO
. VIRUS REPLICATES
cell p÷g ← E.gr:& ⇐ik*←
radioactivity

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✓Radioactivity qoqqwa.a.q.iq ⇐e%aw•aE¥¥
E.gr:-& E.gr:*
p÷g☒÷

DNA is INHERITED ! !