BC-3200

Auto Hematology Analyzer

Service Manual
 Copyright

© 2006 Shenzhen Mindray Bio-medical Electronics Co., Ltd. All rights Reserved.

Intellectual Property Statement

SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS CO., LTD. (hereinafter called
Mindray) owns the intellectual property rights to this Mindray product and this manual. This
manual may refer to information protected by copyrights or patents and does not convey any
license under the patent rights of Mindray, nor the rights of others. Mindray does not assume
any liability arising out of any infringements of patents or other rights of third parties.

Mindray intends to maintain the contents of this manual as confidential information.

Disclosure of the information in this manual in any manner whatsoever without the written
permission of Mindray is strictly forbidden.

Release, amendment, reproduction, distribution, rent, adaption and translation of this manual
in any manner whatsoever without the written permission of Mindray is strictly forbidden.

, are the registered trademarks or trademarks owned by Mindray in China

and other countries. All other trademarks that appear in this manual are used only for editorial
purposes without the intention of improperly using them. They are the property of their
respective owners.

Responsibility on the Manufacturer Party

Contents of this manual are subject to changes without prior notice.

All information contained in this manual is believed to be correct. Mindray shall not be liable
for errors contained herein nor for incidental or consequential damages in connection with the
furnishing, performance, or use of this manual.

Mindray is responsible for safety, reliability and performance of this product only in the
condition that:

„ all installation operations, expansions, changes, modifications and repairs of this product
are conducted by Mindray authorized personnel;

„ the electrical installation of the relevant room complies with the applicable national and
local requirements;

„ the product is used in accordance with the instructions for use.

I
z This equipment must be operated by skilled/trained medical professionals.

z It is important for the hospital or organization that employs this equipment

to carry out a reasonable service/maintenance plan. Neglect of this may
result in machine breakdown or injury of human health.

z Be sure to operate the analyzer under the situation specified in this manual;
otherwise, the analyzer will not work normally and the analysis results will
be unreliable, which would damage the analyzer components and cause
personal injury.

II
Warranty

THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES,

EXPRESSED OR IMPLIED, INCLUDING WARRANTIES OF MERCHANTABILITY OR
FITNESS FOR ANY PARTICULAR PURPOSE.

Exemptions
Mindray's obligation or liability under this warranty does not include any transportation or
other charges or liability for direct, indirect or consequential damages or delay resulting from
the improper use or application of the product or the use of parts or accessories not approved
by Mindray or repairs by people other than Mindray authorized personnel.

This warranty shall not extend to:

„ any Mindray product which has been subjected to misuse, negligence or accident;

„ any Mindray product from which Mindray's original serial number tag or product
identification markings have been altered or removed;

„ any product of any other manufacturer.

Return Policy

Return Procedure
In the event that it becomes necessary to return this product or part of this product to Mindray,
the following procedure should be followed:

1. Obtain return authorization: Contact the Mindray Service Department and obtain a
Customer Service Authorization (Mindray) number. The Mindray number must appear on
the outside of the shipping container. Returned shipments will not be accepted if the
Mindray number is not clearly visible. Please provide the model number, serial number, and
a brief description of the reason for return;

2. Freight policy: The customer is responsible for freight charges when this product is
shipped to Mindray for service (this includes customs charges);

3. Return address: Please send the part(s) or equipment to the address offered by
Customer Service department.

III
Company Contact
Manufacturer: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Address: Mindray Building, Keji 12th Road South, Hi-tech Industrial Park,
Nanshan,ShenZhen 518057, P.R.China,
Phone: +86 755 26582479 26582888

Fax: +86 755 26582934 26582500

IV
Product name: Auto Hematology Analyzer

For this Service Manual, the issued Date is 2006-08 (Version: 1.0).
 Table of Contents

1 Using This Manual ................................................................................... 1-1

1.1 Introduction ............................................................................................ 1-1
1.2 Who Should Read This Manual ............................................................. 1-2
1.3 How to Find Information......................................................................... 1-3
1.4 Conventions Used in This Manual ......................................................... 1-4
1.5 Special Terms Used in This Manual....................................................... 1-5
1.6 Safety Precautions................................................................................. 1-6

2 System Discription .................................................................................. 2-1

2.1 System Overview ................................................................................... 2-1
2.2 Fluidic System........................................................................................ 2-5
2.2.1 Sample Aspiration/Dispensing Module ....................................... 2-5
2.2.2 Reagent Aspiration/Dispensing Module ...................................... 2-6
2.2.3 Hydraulic Module ........................................................................ 2-7
2.2.4 Washing Module.......................................................................... 2-7
2.2.5 Waste Discharging Module ......................................................... 2-9
2.2.6 Counting Module ....................................................................... 2-10
2.2.7 Introduction of Basic Sequence ................................................ 2-10
2.3 Hardware Introduction.......................................................................... 2-23
2.3.1 Circuit Unit................................................................................. 2-23
2.3.2 Volumetric Unit .......................................................................... 2-26
2.3.3 Power Supply Assembly............................................................ 2-27
2.3.4 Panel Assembly......................................................................... 2-27
2.4 Software Introduction ........................................................................... 2-30
2.4.1 Menu Tree ................................................................................. 2-30
2.4.2 Password................................................................................... 2-31

3 Updating the Software............................................................................. 3-1

3.1 Preparation for Update........................................................................... 3-1
3.2 Making Update Floppy Disk ................................................................... 3-1
3.3 Update Procedure.................................................................................. 3-2
3.4 Precautions ............................................................................................ 3-2

4 Repair Procedures................................................................................... 4-1

4.1 Introduction ............................................................................................ 4-1
4.2 Disassembling the Panels...................................................................... 4-2

1
 Table of Contents

4.2.1 Left Door...................................................................................... 4-2

4.2.2 Right Door ................................................................................... 4-2
4.2.3 Front Door ................................................................................... 4-3
4.2.4 Top Cover .................................................................................... 4-5
4.2.5 Back Cover.................................................................................. 4-6
4.3 Replacing the Boards............................................................................. 4-9
4.3.1 Analog Board .............................................................................. 4-9
4.3.2 CPU Board ................................................................................ 4-11
4.3.3 Drive Board ............................................................................... 4-12
4.3.4 Keypad ...................................................................................... 4-14
4.3.5 Indicator Board.......................................................................... 4-16
4.4 Replacing the Power Supply Assembly ............................................... 4-18
4.5 Replacing the LCD............................................................................... 4-20
4.6 Replacing the Recorder ....................................................................... 4-23
4.7 Replacing the Fluidic Components ...................................................... 4-26
4.7.1 Valves........................................................................................ 4-27
4.7.2 Pumps ....................................................................................... 4-29
4.7.3 Syringes .................................................................................... 4-32
4.7.4 Vacuum Chamber ..................................................................... 4-36
4.7.5 Pressure Chamber .................................................................... 4-38
4.7.6 Metering Tube ........................................................................... 4-39
4.8 Replacing the Bath Assembly .............................................................. 4-42
4.8.1 HGB Unit and Aperture of the WBC Unit .................................. 4-42
4.8.2 Aperture of the RBC Unit .......................................................... 4-45
4.9 Replacing the Probe Assembly ............................................................ 4-48
4.9.1 Probe Assembly ........................................................................ 4-48
4.10 Rotation Sensor ................................................................................... 4-52
4.10.1 Elevator Sensor......................................................................... 4-53
4.10.2 Disassembling the Probe Assembly.......................................... 4-54
4.11 Replacing the Sample Compartment Assembly .................................. 4-65
4.11.1 Sample Compartment Assembly and Door............................... 4-65
4.11.2 Electromagnet Assembly........................................................... 4-66
4.11.3 Disassembling the Sample Compartment Assembly ................ 4-69
4.12 Adjusting the Sample Probe Position .................................................. 4-75
4.12.1 Start Position ............................................................................. 4-75
4.12.2 Pre-sample Position .................................................................. 4-75
4.13 Adjusting the Rotation Position of the Sample Probe .......................... 4-77
4.14 Adjusting the Gain................................................................................ 4-79

5 Troubleshooting....................................................................................... 5-1
5.1 Error Codes............................................................................................ 5-1
5.2 Troubleshooting ..................................................................................... 5-2
5.2.1 Pressure errors ........................................................................... 5-2
5.2.2 Reagent errors ............................................................................ 5-3

2
 Table of Contents

5.2.3 Hardware errors .......................................................................... 5-3

5.2.4 Power supply errors .................................................................... 5-6
5.2.5 Measurement errors.................................................................... 5-6
5.2.6 External connection errors .......................................................... 5-8
5.2.7 Ambient temperature error .......................................................... 5-9
5.2.8 Other errors ................................................................................. 5-9
5.3 Using Pulse Graphs to Troubleshoot the Analyzer .............................. 5-10
5.3.1 Normal pulse graph................................................................... 5-10
5.3.2 Abnormal pulse graph ............................................................... 5-11
5.4 Common troubleshooting procedures.................................................. 5-15

6 List of Spare Parts ................................................................................... 6-1

6.1 Spare PCBA ........................................................................................... 6-1
6.2 Spare Parts ............................................................................................ 6-2
6.3 Consumables ......................................................................................... 6-3
6.4 Tubing .................................................................................................... 6-4
6.4.1 Main fluidic tubing connection table ............................................ 6-4
6.4.2 Assembly tubing connection table............................................... 6-6
6.4.3 Accessory tubing connection table ............................................. 6-8
6.4.4 Tube type table............................................................................ 6-9
6.4.5 Connecter table ........................................................................... 6-9
6.5 Cable.................................................................................................... 6-10

7 Appendices ..............................................................................................A-1
A Fluidic Diagram ......................................................................................A-1
B Electric Connection Diagram .................................................................B-1
C Test Point of Hardware Board ................................................................C-1
C.1 CPU board test point...................................................................C-1
C.2 Analog test point .........................................................................C-3
C.3 Drive board test point ..................................................................C-4
D Valve Test ...............................................................................................D-1

3
 1 Using This Manual

1.1 Introduction

This chapter explains how to use your BC-3200 service manual, which provides the reference
information and procedures needed for servicing your analyzer. Read this manual carefully
before servicing your analyzer and service your analyzer strictly as instructed in this manual.

This manual is to be used in conjunction with the BC-3200 operation manual and does not
contain information and procedures already covered in the operation manual.

z Be sure to service your analyzer strictly as instructed in this manual and the
operation manual.

1-1
 Using This Manual

1.2 Who Should Read This Manual

This service manual is written for people who

„ have a thorough understanding of electronic and fluidic principles.

„ have a thorough understanding of reagent systems.

„ have a thorough understanding of quality control.

„ have a thorough understanding of troubleshooting concepts.

„ have an operator’s knowledge of the analyzer.

„ have the ability to use basic mechanical tools and understand related terminology.

„ have the ability to use a digital voltmeter (DVM) and an oscilloscope.

„ have the ability to read electronic and fluidic schematics and understand related
terminology.

1-2
 Using This Manual

1.3 How to Find Information

This service manual comprises 6 chapters and 4 appendices. Refer to the table below to find
the information you need.

If you want to … See …

learn about the sub-systems of the BC-3200 analyzer and how Chapter 2 System Discription

they function.
learn about how to install the BC-3200 and update the Chapter 3 Installation and
Updating
software.
learn about the procedures to repair the BC-3200. Chapter 4 Repair Procedures

learn about the procedure to troubleshoot the BC-3200. Chapter 5 Troubleshooting

learn about the main parts of the BC-3200. Chapter 6 Part List

learn about the fluidic diagram of the BC-3200. Appendix A

learn about the hardware boards of the BC-3200. Appendix B

learn about the jumpers and testing points of the BC-3200. Appendix C

learn about the valves of the BC-3200. Appendix D

1-3
 Using This Manual

1.4 Conventions Used in This Manual

This manual uses certain typographical conventions to clarify meaning in the text:

„ All capital letters enclosed in [ ] indicate a key name (either on the built-in keypad or the
external keyboard), such as [ENTER].

„ All capital, bold and italic letters indicate a special operation defined in the following
section, such as SELECT.

„ Bold letters included in “ ” indicate text you can find on the screen, such as “Prepare to
ship”.

„ Bold letters indicate defined screen areas/fields, such as System Status area, or
chapter titles, such as Chapter 1 Using This Manual.

All illustrations in this manual are provided as examples only. They may not necessarily
reflect your analyzer setup or data displayed.

1-4
 Using This Manual

1.5 Special Terms Used in This Manual

When you read … It means …

to press the arrow keys ([←][→] [↑][↓]) as needed to move the

CLICK cursor to a certain software button on screen and press
[ENTER].
to press the arrow keys ([←][→] [↑][↓]) as needed to move
cursor to the desired edit box and use the built-in keypad or
the external keyboard to enter the desired characters or digits.
ENTER
Note that besides the numeric keys you may also use the
[PgUp] or [PgDn] keys to enter digits; or
to scan the number in using the bar-code scanner.
to press the arrow keys ([←][→] [↑][↓]) as needed to move the
cursor to the character or digit to the left of the one you want to
delete and press [DEL]; or
DELETE
to press the arrow keys ([←][→][↑][↓]) as needed to move the
cursor to the character or digit to the right of the one you want
to delete and press [BackSpace] on the external keyboard.

to move the cursor to the character or digit you want to change

MODIFY and re-enter the desired one using either the built-in keypad or
the external keyboard or the bar-code scanner.
to press the arrow keys ([←][→] [↑][↓]) as needed to move the
SELECT from “ ** ”
cursor to the desired edit box and press [ENTER] to display
pull-down list
the pull-down list and press [↑] or [↓] to move the cursor to the
desired item and press [ENTER] to select it.
to press the arrow keys ([←][→] [↑][↓]) as needed to move the
SELECT
cursor to the desired item and press [ENTER].

z This analyzer adopts a fixed decimal point. You can enter the digits without
bothering to look for the [.] on the external keyboard.

1-5
 Using This Manual

1.6 Safety Precautions

You will find the following symbols in this manual.

When you see… Then…

read the statement below the symbol. The statement is

alerting you to an operating hazard that can cause
personnel injury.
read the statement below the symbol. The statement is
alerting you to a possibility of analyzer damage or unreliable
analysis results.
read the statement below the symbol. The statement is
alerting you to information that requires your attention.

read the statement below the symbol . The statement is

alerting you to a potentially biohazardous condition.

You may find the following symbols on the analyzer or the reagents.

When you see… It means…

EQUIPOTENTIALITY

CAUTION, CONSULT ACCOMPANYING

DOCUMENTS.

BIOLOGICAL RISK

HIGH VOLTAGE

ALTERNATING CURRENT

FOR IN VITRO DIAGNOSTIC USE

1-6
 Using This Manual

BATCH CODE

USE BY

SERIAL NUMBER

DATE OF MANUFACTURE

TEMPERATURE LIMITATION

CONSULT INSTRUCTIONS FOR USE

MANUFACTURER

IRRITATING SUBSTANCE

Safety precautions:
Please pay attention to the following precautions to insure the personal safety.

z It is important for the hospital or organization that employs this equipment

to carry out a reasonable service/maintenance plan. Neglect of this may
result in machine breakdown or injury of human health.

z Power off the device when servicing it. Otherwise electric shock or damage
to electronic parts may occur.

z Connect the device to the socket which has a separate fuse and protection
switch. Otherwise the device may be tripped in case of failure, over current
or impact current at startup.

z To avoid personal injury, be sure to keep your clothes, hair and hand away
from such moving parts as the sample probe.

z The warning parts will move mechanically and may cause injury during
normal operation or disassembly or servicing.

1-7
 Using This Manual

z Be sure to dispose of reagents, waste, samples, consumables, etc.

according to government regulations.

z The reagents are irritating to eyes, skin and diaphragm. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe
laboratory procedures when handling them in the laboratory.

z If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.

z Improper servicing may damage the device. Service it following the

instructions and check the device thoroughly after servicing.

z In the event of conditions not indicated in the service manual, contact

Mindray customer service department for professional suggestions.

z When maintaining the electronic parts of the analyzer, be sure to remove the
metallic decorations you are wearing so as to avoid damage to you and the
analyzer.

z Static discharging may damage the electronic parts, be sure to wear

antistatic hand rings while maintaining or perform maintenance on the
antistatic platform.

z Close the sample compartment when opening the front door, otherwise you
may destroy them.

z This equipment must be operated by skilled/trained medical professionals.

z The probe tip is sharp and may contain biohazardous materials. Exercise
caution to avoid contact with the probe when working around it.

z Samples, controls, calibrators and waste are potentially infectious. Wear

proper personal protective equipment (e.g. gloves, lab coat, etc.) and follow
safe laboratory procedures when handling them in the laboratory.

z All the analyzer components and surfaces are potentially infectious, take
proper protective measures for operation or maintenance.

1-8
 2 System Discription

2.1 System Overview

„ Hardware

The hardware system controls/drives the valves, pumps and motors, converts the detected
blood cell signals into input signals as required, counts the number of cells, and provides
control/drive and monitoring signals.

„ Interface

The BC-3200 aspirates blood sample in an enclosed way. Press the [OPEN] key, as shown in
Figure 2-1, to open the sample compartment and close it after putting in the sample tube.
Press the [ASPIRATE] key. Then the device starts analyzing the sample and displays the
results (including graphs/tables and data) on the screen. The recorder prints analysis reports
and interested information in a couple of formats. Moreover, the keypad is used to input
operating instructions and parameters.

There are several external interfaces on the rear panel of the device, as shown in Figure 2-2,
which include two RS-232 serial ports, a parallel port, a keypad interface and a power
interface for floppy disk drive. The two RS-232 serial ports are used for communication with
an external PC and a bar code reader respectively. The parallel port is to connect a printer or
a floppy disk drive when updating the system.

2-1
 System Discription

Figure 2-1 Front view

1 --- LCD 2 --- Keypad

3 --- Recorder 4 --- Power indicator
5 --- [OPEN] key 6 --- [ASPIRATE] key
7 --- Sample compartment

2-2
 System Discription

Figure 2-2 Back view

1 --- Parallel port 2 --- RS-232 Port1

3 --- RS-232 port2 4 --- Keyboard interface
5 --- Power Interface of floppy disk drive 6 --- Safety labeling
7 --- Diluent inlet 8 --- Diluent sensor connector
9 --- Rinse sensor connector 10 --- Waste outlet
11 --- Waster sensor connector 12--- Rinse inlet
13--- Power switch 14--- Equipotentiality

„ Software Running Environment

The analyzer is equipped with a 32Bit Motorola ColdFire as its primary processor. The BIOS
and FPGA data and the boot program of the system software are stored in a 1MB NOR Flash.
The system software and sample information are stored in the Disk-on-Drive (DOM). During
startup of the analyzer, the hardware automatically loads the boot program which runs in the
flash and configures the FPGA, drives the keypad, turns on the LCD backlight, loads the
system software and finally transfers the CPU control to the system software. The system
software runs in a 16MB SDRAM, of which 1M is for the software code and the other 15M is
used as a memory during running.

2-3
 System Discription

„ Fluidic System

The fluidic system is used for reagents, samples and air to flow inside the device. The fluidic
components are interconnected according to certain relationship and controlled by the
software and hardware based on the given timing, so that the distribution and flow direction of
the liquid are controlled.

2-4
 System Discription

2.2 Fluidic System

2.2.1 Sample Aspiration/Dispensing Module

V1 V4 V5 V6 V3

RB
C WBC
Bat Bath 2.5mL 10mL
h Syringe Syringe
50uL DILUENT
Syringe

LYSE

Figure 2-3 Sample Aspiration/Dispensing Module

Sample is dispensed in two modes: whole blood and predilute. 13µL venous blood is
aspirated in whole blood mode and 0.3mL prediluted capillary blood in predilute mode.

In whole blood mode, the sample is aspirated by the 50µL syringe and then stored in the
probe.

In predilute mode, the sample is aspirated by the 10mL syringe. The probe lowers down to
the 1.5mL centrifugal tube which holds the prediluted sample. V3 and V4 are opened. The
10mL syringe then starts aspirating the sample, which enters the probe and then the tube
connected to it under the action of pressure. When enough sample volume is aspirated, the
syringe stops and V4 is closed. The aspirated sample is stored in the probe and the tube
connected to it. To prevent the sample from entering V4 and the syringe, a tube with larger
capacity than the aspiration volume can be used.

The sample is dispensed from the probe to the bath. Blood is viscous and may adhere to
interior of the probe and tube. The sample needs to be diluted with defined volume of diluent,
which is dispensed through the probe to rinse the sample remained on the probe/tube wall
while the sample is dispensed. The 10mL syringe is used for diluent dispensing. Since
resistance may occur due to smaller inner diameter of the probe, the syringe dispenses fluid
slowly, thus preventing the tube from disconnecting owing to excessive compressed air and
reducing bubbles.

The probe is a moving part and can move vertically and horizontally. The tube connected to it
also changes in shape (bending or straightening) while it moves. The shape change may

2-5
 System Discription

influence the tube capacity and even the sample aspiration. To minish the tube shape change
without increasing the flow resistance, a rigid EVA tube is used between the probe and the
50uL syringe and a relatively flexible Teflon tube is used between the 50uL syringe and valve
4.

2.2.2 Reagent Aspiration/Dispensing Module

Reagent dispensing involves in diluent/lyse in common status and probe/E-Z cleanser in
maintenance status.

Usually, the diluent volume is 0.5mL - 6mL and aspirated by the 10mL syringe through the
diluent inlet. As shown in Figure 2-3, since only V3 is connected between the 10mL syringe
and the diluent inlet, small resistance may occur and diluent can be aspirated in not-too-fast
speed to avoid bubbles. To better decrease the resistance, a tube with large inner diameter
(R-3603 ID1/4”) can be used to connect to the diluent inlet. The diluent may be dispensed to
the probe, probe wipe, bath or tubes. When the 10mL syringe aspirates enough diluent,
V1/V3 are opened, then the syringe dispenses certain diluent volume to the probe wipe to
wash the exterior of the probe. When the probe rotates to a bath, V3/V4 are opened while the
syringe is dispensing, then the diluent is dispensed to the bath. During this process, the
exterior of the probe is washed. Moreover, by opening only the V3 or both V3 and V4, the
diluent can be dispensed to a bath through the tube connected to it.

The lyse volume is usually 0.3-1mL and aspirated/dispensed through the cooperation of the
2.5mL syringe and V6. When certain volume of lyse is aspirated through the lyse inlet, V6 is
opened and the aspirated lyse is dispensed to the WBC bath through the lyse tube connected
to the bath.

The probe/E-Z cleanser volume is 1-2mL and aspirated through the probe and dispensed to
the bath through the 10mL syringe. Cleanser is aspirated/dispensed in the same way as
sample in predilute mode. The tube connected to the probe should have a larger capacity
than the aspiration volume, so that the cleanser will not be absorbed into valves and syringes.

2-6
 System Discription

2.2.3 Hydraulic Module

Pressure Pressure
sensor sensor

Pressur Vacuum chamber

e
chamber
C
V9
V10
NO
NC
Pneumatic
pump

Pump

Waste

Figure 2-4 Hydraulic module

The fluid flow of the BC-3200 is implemented by not only the syringes, but also drive from the
air and compressed air. To establish the air, vacuum is taken by using the vacuum chamber
along with a pump and V10. The compressed air is established by using the pressure
chamber and the pneumatic pump. The above-mentioned two chambers are connected
respectively to a pressure sensor, which detects the vacuum and pressure status, as shown
in Figure 2-4.

The pneumatic pump is used to establish compressed air. The air flows from atmosphere to
the pressure chamber. When V10 and the pump are opened, the air flows from vacuum
chamber to the waste outlet, and then the air is established. When compressed air needs to
be stored in the vacuum chamber, the normally-closed terminal of V9 is opened after
compressed air’s establishment in the pressure chamber, and then the air flows from the
pressure chamber to the vacuum chamber.

2.2.4 Washing Module

The washing module deals with washing the interior/exterior of the probe, front/back baths
and the tubes, and flushing the apertures.

Probe Washing Module

To wash the interior of the probe, the probe moves to the bath, V3/V4 are opened while the
10mL syringe is dispensing diluent, which enters in the bath through the probe. To wash the
exterior of the probe, V3/V1 are opened while the 10mL syringe is slowly dispensing diluent,
which enters from the wipe inlet. The wipe outlet is connected to a pump through a valve.

2-7
 System Discription

When the syringe starts dispensing diluent to the wipe, the pump and V2 are opened to
discharge the waste of the probe wipe. The probe, including its tip, is washed by relative
movement of the probe and the probe wipe. When diluent dispensing is stopped, the waste
outlet is still taking out air so that the exterior of the probe is dried, as shown in Figure 2-5.

Figure 2-5 Probe washing module

Front/Back Bath Washing Module

Figure 2-6 Front/back bath washing module

To wash the front bath, when the 10mL syringe aspirates enough diluent, V3 is opened alone

2-8
 System Discription

to dispense the diluent to the WBC front bath; or both V3 and V5 are opened to drive the
syringe to dispense the diluent to the RBC front bath. The front bath should be emptied
before dispensing diluent to it. The diluent volume should be greater than the maximum
sample volume in the bath during analysis. The front bath is connected to a pump through an
isolation chamber and a valve, which is closed when diluent is dispensed in the front bath,
and opened when the waste in the bath is being discharged by the pump, as shown in Figure
2-6.

The back bath is washed with rinse and has an inlet and outlet. The inlet is connected to the
rinse inlet through V13/V14, while the outlet shares a tube with the analyzing channel, as
shown in Figure 2-6. When V8 and V18 are opened simultaneously, the rinse inlet is
interconnected to the vacuum chamber, and the rinse enters the vacuum chamber under the
action of compressed air to wash the back bath.

Since the outlet of the front bath needs to be isolated to avoid interference signals, an
isolation chamber is added at the outlet.

2.2.5 Waste Discharging Module

V7
V8

V17
V18

RBC WBC
Bath Bath

V14
Rinse

Vacumm
Chamber
Prob Isolation Isolation V13
e Chambe Chambe
r r

Probe Wipe
Housing

V2 V10 V11 V12

Waste
Pump

Figure 2-7 Waste discharging module

The waste discharging module deals with discharging the waste from the probe wipe, baths
and the vacuum chamber.

The waste of the front/back baths and the vacuum chamber is discharged through a pump.
To discharge the waste of the WBC/RBC front bath, V11 or V12 is opened while the pump is
opened. It is relatively complicated to wash the back bath. Certain vacuum should first be

2-9
 System Discription

established in the vacuum chamber, and then V13/V14/V8/V18 are opened. The rinse enters
in the vacuum chamber through the back bath under the action of pressure. The back bath is
rinsed. During this process, the pump and V10 are opened to discharge the waste of the
vacuum chamber. To discharge waste in the probe wipe, V2 and the pump are opened.
The waste discharging module is shown in Figure 2-7.

2.2.6 Counting Module

The counting module deals with cells counting and volumetric metering. This module includes
the front/back baths, with an aperture between them. A washer is applied to the aperture for
encapsulation. The front bath is connected to the front electrode (positive) and the back bath
to the back electrode (ground). The back bath connects to the vacuum chamber through a
valve, which is opened during sample analysis. Liquid is driven by the air to flow to the back
bath through the front bath. See Figure 2-8.

Figure 2-8 Counting module

As shown in Figure 2-8, in normal status, tube “a” is for liquid (rinse) and tube “b” is for air.
Liquid must not enter V7/V17 and the filters connected to them. Tube “c” is filled with liquid
(rinse) in non-measuring status. Before analysis begins, V7 and V17 are opened to discharge
the liquid in tube “c” by taking in air. Meanwhile, the photo-coupler on the metering tube is
isolated. When analysis begins, V8 and V18 are opened, and liquid flows towards the
vacuum chamber through tube “a” and tube “c”. Corresponding photo-couplers are opened
when the liquid flows to them.

2.2.7 Introduction of Basic Sequence

1. Movement of the sample probe: The sequence commands the hardware to control the
elevation and rotation of the sample probe. The hardware first tells the right position of the

2-10
 System Discription

tube and then controls the elevation of the sample probe.

2. To clean the sample probe: The probe is cleaned when it goes up and down. V1 valve
and V3 valve are opened and the 10mL syringe dispenses fluid. V2 valve is opened and
the waste pump starts working. Usually the washing is finished in 2s.

3. To establish vacuum: To establish vacuum, V10 valve is opened and the waste pump
starts working. The vacuum pressure value can be controlled in two ways: 1) To be
controlled by the software (vac: start time/end time, upper/lower limit of the vacuum); 2) To
be controlled directly with V10 valve opened and the waste pump starts working. When
counting, the vacuum pressure must be -24kPa.

4. To establish pressure: To establish pressure, the pressure pump starts working. The
pressure value is controlled by software (press: start time/end time, upper/lower limit of the
pressure). It is used for two purposes: 1) To pump bubbles into baths for mixing the sample
(controlled by press); 2) To increase the pressure in vacuum chamber to flush the aperture,
which is implemented by starting the pressure pump and opening V9 valve.

5. To aspirate diluent: Diluent is aspirated via the 10mL syringe.

6. To dispense diluent: With the 10mL syringe and V3 valve opened, diluent is dispensed
into the WBC bath, RBC bath or to the probe wipe.

7. To dispense diluent to the probe wipe: When both V3 valve and V1 valve are opened
simultaneously, diluent is dispensed to the probe wipe.

8. To dispense diluent to the sample probe: When V3 valve, V1 valve and V4 valve are
opened simultaneously, diluent is dispensed into the sample probe.

9. To dispense diluent to the RBC bath: When V3 valve, V1 valve, V4 valve and V5 valve
are opened simultaneously, diluent is dispensed into the RBC bath.

10. To aspirate lyse: Lyse is aspirated via the 2.5mL syringe.

11. To dispense lyse: With the 2.5mL syringe and V6 valve opened, lyse is dispensed into
the WBC bath.

12. To empty the RBC bath: With the waste pump started and V11 valve opened, the RBC
bath is emptied. Usually it finished in 2s.

13. To empty the WBC bath: With the waste pump started and V12 valve opened, the WBC
bath is emptied. Usually it finished in 2s.

14. To pump air into the RBC bath: Certain pressure is first established in the pressure
chamber through press command and then V15 valve is opened, thus the air in the
pressure chamber is pumped into the RBC bath.

15. To pump air into the WBC bath: Certain pressure is first established in the pressure
chamber through press command and then V16 valve is opened, thus the air in the
pressure chamber is pumped into the WBC bath.

16. To aspirate the sample: Sample is aspirated via the 50µL syringe.

17. To dispense the sample: With the 10mL syringe and V4 valve opened, sample is
dispensed from the sample probe. Meanwhile, a little diluent is also dispensed to rinse the
interior of the sample probe.

2-11
 System Discription

18. To empty the RBC metering tube: After vacuum is established, V17 valve is opened to
empty the RBC metering tube.

19. RBC counting: After the RBC metering tube is emptied and vacuum is established, V18
valve is opened to start counting.

20. To empty the WBC metering tube: After vacuum is established, V7 valve is opened to
empty the WBC metering tube.

21. WBC counting: After the WBC metering tube is emptied and vacuum is established, V8
valve is opened to start counting.

22. To clean the RBC back bath and the RBC metering tube: After vacuum is established,
V13 valve and V18 valve are opened so that rinse can pass through the RBC back bath
and the RBC metering tube and then to the vacuum chamber.

23. To clean the WBC back bath and the WBC metering tube: After vacuum is established,
V14 valve and V8 valve are opened so that rinse can pass through the WBC back bath and
the WBC metering tube and then to the vacuum chamber.

Sequence of dispensing diluent

Fluidic Sequence Description

2.9mL of diluent is The sample To aspirate the accurate sample volume, the
aspirated and the M1 probe is sample probe aspirates diluent to empty the
motor is replaced. lowered and its probe at level 5.
exterior is
dried.
0.9mL of diluent is dispensed into the 0.9mL of diluent is dispensed into the collection
collection tube by the sample probe. tube to ensure enough sample volume for the
second aspiration.
The sample probe is raised and its exterior The M2 motor controls the dispensesing at level
is cleaned with 2mL of diluent. 1 to avoid the probe wipe leaking. V2 valve is
closed 0.5s later than V1 valve and V3 valve to
dry the probe tip.
50µL syringe re-aspirates The sample When the analysis is finished, 50µL syringe
2µL of diluent. compartment re-aspirates 2µL of diluent to avoid liquid
door is accumulating at the probe hole and dripping.
opened.

2-12
System Discription

2-13

Sequence of preparation for whole blood counting
Fluidic Sequence
1.3mL of diluent is aspirated.
The exterior of the sample
probe is cleaned.
6mL of diluent is aspirated.
4mL of diluent is dispensed
into the WBC bath and 2
bubbles are pumped into the
bath. The vacuum chamber is
emptied.
The sample probe is raised and its exterior is cleaned slowly
with 2mL of diluent.
The sample probe aspirates
6mL of diluent and then is
turned left.
The sample probe is lowered
and then dispenses 1.6mL of
diluent into the WBC bath.
2mL of prediluted sample is
dispensed via the sample
probe movement.
1.5mL of diluent is aspirated.
50µL syringe is replaced.
4mL of diluent is added into
the RBC bath. Bubbles
pumped helps to mix the
sample.
5.6 mL of diluent is aspirated.
System Discription
The sample probe is lowered.
The WBC bath is emptied.
50µL syringe aspirates 13µL
of sample.
The WBC bath and the
metering tube are emptied.
The RBC bath is emptied.
-8/ -24kPa vacuum is
established.
15.6 µL of the second sample
is aspirated.
2-14
Description
The sample probe is lowered
twice to pierce the rubber cap
of the collection tube, which
helps to better release the
vacuum inside.
The diluent, aspirated at level
5, is used to clean the
exterior of the sample probe
and the bath.
The syringe aspirates the
sample slowly. Bubbles
pumped into the WBC bath
helps to better clean it.
The 10mL syringe dispenses
diluent at level 1 and the
probe is dried for 0.5s.
It takes 2.2s to empty WBC
bath. The metering tube is
emptied, to ensure a normal
analysis cycle.
1.6mL of diluent is dispensed
into the bath so that the
probe hole can be immerged
into the diluent to avoid spills
and bubbles.
The whole blood sample is
flushed out at level 2 with
2mL of diluent to flush the
interior of the sample probe.
50µL syringe is replaced
slowly to avoid bubbles in the
bath and to prepare for the
second aspiration. Bubbles
pumped into the RBC bath
helps to clean it better.
15.6 µL of sample is
aspirated slowly for RBC
counting.
 System Discription

The sample probe is raised and the exterior of the probe is The exterior of the probe is
cleaned with 1.6mL of diluent. cleaned with 1.6mL of diluent
for 2s and dried for 0.7s.
1.6mL of diluent is added into The sample probe is lowered. 1.6mL of diluent is added into
the RBC bath. the bath to avoid spills and
bubbles when the probe is
dispensing sample.
1mL of prediluted 0.5mL of lyse is -22.5/ -24kPa Diluent and sample are
sample is dispensed into the vacuum is dispensed into the RBC bath
dispensed into the WBC bath and 10 established. through the sample probe at
WBC bath via the bubbles are level 2. The interior of the
sample probe pumped into the probe is cleaned at the same
moving. bath. time. Bubbles pumped into
the bath help to well mix the
sample and established
vacuum for counting.

Sequence of preparation for predilute counting

Fluidic Sequence Description

4mL of diluent is aspirated.
The WBC bath is emptied.
4mL of diluent is added into
the WBC bath.
The sample probe aspirates
0.3mL of sample.
2mL of diluent is aspirated.
The sample probe is raised and its exterior is cleaned with
2mL of diluent.
4.3mL of diluent is aspirated.
The sample probe is turned
right.
1.6mL of diluent is added into
50µL syringe re-aspirates 2µL
of diluent. The sample probe
is lowered.
2 bubbles are pumped into
the WBC bath.
The WBC bath is emptied.
The metering tube is emptied.
The RBC bath is emptied.
The sample probe is lowered.
The sample probe is lowered
900 steps per second, which
takes 2s.
When diluent is dispensed
into the WBC bath, bubbles
pumped into the bath helps to
clean.
0.9mL of diluent is dispensed
into the collection tube to
ensure enough sample
volume for the second
aspiration. To aspirate
accurate sample volume,
10mL syringe aspirates
sample slowly.
To avoid the probe wipe
leaking, the syringe
dispenses diluent at level 1.
Diluent is aspirated at level 5.
To ensure a normal
measurement cycle, the
metering tube is emptied.
1.6mL of diluent is added into

2-15
 System Discription

the WBC bath. the bath so that the probe

3mL of diluent is aspirated. hole is well into the diluent to
avoid spills and bubbles.
1.6mL of prediluted sample is dispensed into the WBC bath The prediluted sample is
via the sample probe moving. flushed out and the interior of
the sample probe is cleaned
with 1.3mL of diluent slowly.
The diluent is flushed at level
3 here.
3mL of diluent is added into 2 bubbles are pumped into 50µL syringe is replaced
the RBC bath. The M1 motor the RBC bath and 2 into the slowly to avoid bubbles in the
is replaced. WBC bath. bath and prepare for the
second aspiration. Bubbles
pumped into the RBC bath
helps to clean, while into the
WBC bath helps to well mix
the prediluted sample before
the second aspiration.
4mL of diluent is aspirated. Meanwhile, the 50µL syringe Prediluted sample aspirated
aspirates 30µL of prediluted here is for RBC counting. The
sample. motor aspirates the sample
slowly. 30µL of prediluted
sample meets the basic
dilution requirement.
The exterior of the sample The sample probe is raised. The sample probe is cleaned
probe is cleaned with 1.6mL with 1.6mL of diluent at level
of diluent. 1 for 2s and dried for 0.5s.
0.6mL of diluent is aspirated. The sample probe is turned The sample probe is placed
right. over the RBC bath.
1.6mL of diluent is added into the RBC -22.5 / -24kPa 1.6mL of diluent is added into
bath. And The sample probe is lowered. vacuum is the bath to avoid spills and
established. bubbles when the probe is
dispensing sample.
1.2mL of 1.2mL of The dispensation of
prediluted sample prediluted sample prediluted sample helps to
is dispensed into is dispensed into clean the interior of the
the RBC bath via the RBC bath via sample probe and the motor
the sample probe the sample probe dispenses sample at level 2.
moving. moving. Bubbles pumped into the
WBC bath helps to well mix
the sample with the lyse and
establish the vacuum for the
counting.
The sample probe is raised The M1 motor is replaced. The sample probe is cleaned

2-16
 System Discription

and its exterior is cleaned with 1.6mL of diluent at level

with 1.6mL of diluent. 3 1 and dried for 0.8s. Bubbles
bubbles are pumped into the pumped into the RBC bath
RBC bath to mix the sample. helps to pump the sample in
the tubing back into the bath.
A positive pressure of 1.5/2.5kPa is kept established.

2-17
 System Discription

Counting Sequence

Fluidic Sequence Description

The RBC counting The HGB Valve 18 is opened and the RBC counting
starts. measurement starts. starts. The HGB measurement starts.
The WBC counting starts. Valve 8 is opened 6s later than valve 18 and
the WBC counting starts.

Sequence of post-cleaning

Fluidic Sequence Description

4.5mL of diluent is The WBC bath is The WBC bath is cleaned.
aspirated. emptied.
The sample compartment door opens When the analysis is finished, the result will
automatically. be displayed on the screen. The sample
compartment door will automatically open
and the sample tube can be removed.
4.5mL of diluent is The RBC back bath is Since 4.5mL of diluent added is higher than
added into the flushed. the original liquid in the bath, it helps to
WBC bath. wash away the rest sample in the WBC
bath. The RBC back bath is flushed directly
with rinse pumped by the liquid pump.
4mL of diluent is The RBC bath is emptied The zapping here is done for the aperture of
aspirated. and the aperture is the WBC bath.
zapped.
4mL of diluent is The WBC The Since 4mL of diluent added is higher than
added into the RBC back bath aperture is the original fluid in the bath, it helps to wash
bath. is flushed. zapped away the rest sample in the RBC bath. The
The sample probe WBC back bath is flushed directly with rinse
is lowered into the pumped by the liquid pump.
RBC bath.
50µL syringe The sample 1s after diluent is added into the RBC bath,
re-aspirates 2µL of probe is the zapping starts and lasts for 4S. When
diluent. raised and the sample probe is raised, V2 valve and
the exterior fluidic pump open to dry the exterior of the
of sample sample probe. 50µL syringe re-aspirates
probe is 2µL of diluent to avoid liquid accumulating at
dried. the probe hole and dripping.
The sample probe is replaced to the middle The sample probe is replaced to the
position. pre-sampling position for the next analysis.

2-18
System Discription

2-19
 System Discription

Startup Sequence after Normal Shutdown

Fluidic Sequence Description

4mL of diluent is The sample WBC bath 10mL syringe aspirates diluent at
aspirated probe is is emptied. level 5; the sample probe is lowered
lowered. above the tube position.
The exterior of the The sample probe is raised. To avoid probe wipe leaking, 10mL
sample probe is syringe aspirates diluent at level 1.
cleaned with 2mL of
diluent
4mL of diluent is The sample probe is turned 10mL syringe aspirates diluent at
aspirated. left. level 5. The sample probe is placed
above the WBC bath.
4mL of diluent is The sample probe is Diluent is added into the WBC bath
added into the WBC lowered. through dispensing tube. The sample
bath. probe is lowered into the WBC bath.
4mL of diluent is aspirated. WBC bath 10mL syringe aspirates diluent at
is emptied. level 5.
2mL of diluent is added into the WBC RBC bath is Diluent is added into the WBC bath
bath. emptied. through dispensing tube.
The interior of the sample probe is -12 / -8 kPa Diluent is added into the WBC bath
cleaned with 2mL of diluent. vacuum is through sample probe and the probe
established. is cleaned at the same time. 10mL
syringe aspirates diluent at level 4.
4mL of diluent is aspirated. 10mL syringe aspirates diluent at
level 5.
4mL of diluent is 4 bubbles are Diluent is added into the RBC bath
added into the RBC pumped into through dispensing tube. Bubbles
bath. the RBC pumped helps to clean the RBC bath.
bath.
3.6mL of diluent is RBC bath is emptied. 10mL syringe aspirates diluent at
aspirated. level 5.
4mL of diluent is added into the RBC bath. Diluent is added into the RBC bath
through dispensing tube.
The exterior of the The sample probe is raised. To protect the probe wipe from
sample probe is leaking, 10mL syringe aspirates
cleaned with 1.6mL of diluent at level 1.
diluent.
The two back baths The sample probe is Rinse pumped by the fluidic pump
are cleaned. replaced to the middle flushes the back baths. The sample
position. probe is replaced to the sampling
position.
4mL of diluent is WBC bath is emptied. 10mL syringe aspirates diluent at

2-20
 System Discription

aspirated. level 5.

4mL of diluent is 4 bubbles are WBC bath Diluent is added into the WBC bath
added into the WBC pumped into is emptied. through dispensing tube. Bubbles
bath. the WBC pumped helps to clean the WBC
bath. bath.
4mL of diluent is aspirated. 10mL syringe aspirates diluent at
level 5
4mL of diluent is added into the RBC bath. Diluent is added into the RBC bath
through dispensing tube.
The back bath is rinsed. Rinse pumped by the fluidic pump
flushes the back bath.
5mL of diluent is 0.5mL lyse is WBC bath 10mL syringe aspirates diluent at
aspirated. aspirated. is emptied. level 5. 50µL syringe aspirates lyse
quickly.
5mL of diluent is added into the WBC bath. Diluent is added into the WBC bath
through dispensing tube.
5mL of diluent is aspirated. WBC bath 10mL syringe aspirates diluent at
is emptied. level 5.
5mL of diluent is added into the WBC bath. Diluent is added into the WBC bath
through dispensing tube.
0.5mL of lyse is added 4 bubbles are RBC bath is 50µL syringe adds lyse quickly into
into the WBC bath. pumped into emptied. the WBC bath through the dispensing
the WBC tube. Bubbles pumped helps to mix
bath. diluent and lyse.
5mL of diluent is WBC bath is emptied. 10mL syringe aspirates diluent at
aspirated. level 5.
5mL of diluent is Vacuum chamber is Diluent is added into the WBC bath
added into the WBC emptied. through dispensing tube.
bath.
5mL of diluent is aspirated. Pressure in 10mL syringe aspirates diluent at
the vacuum level 5.
5mL diluent is added The exterior chamber is Diluent is added into the RBC bath
into the RBC bath. of the sample resumed. through dispensing tube. 50µL
50µL syringe probe is syringe re-aspirates 2µL of diluent to
re-aspirates 2µL of dried. The avoid liquid accumulating at the
diluent. aperture is probe hole and dripping. 110V
zapped. voltage is added at ends of the
aperture to zap the aperture. The
vacuum chamber and pressure
chamber are connected to release
the vacuum.

2-21
System Discription

2-22
 System Discription

2.3 Hardware Introduction

According to the mechanical design, the BC-3200 is divided into circuit unit, volumetric unit,
power supply and panel assemblies.

2.3.1 Circuit Unit

The circuit unit includes CPU board, analog board and drive board, which function as follows:
CPU board: Key part of the circuit unit. Receives instructions input from the keypad; calls the
fluidic timing; sends instructions to the drive board; receives analog signals; monitors the
system parameters; and displays the test results on the screen.

Analog board: Converts the signals output from each sensor into required digital signals;
provides control/drive and monitoring signals.

Drive board: Drives the valves, pumps and motors of the system; and carries out the
instructions sent by the CPU.

CPU Board
„ Working Principles

CPU, FPGA and Super I/O are the major parts on this board. The CPU carries out
instructions and functions as the core of the board. The FPGA acts as a logical interface
between the CPU and the Super I/O. The super I/O includes various interfaces that can be
accessed by the CPU through the LPC of FPGA. SDRAM is the system memory. FLASH is
where the boot software and FPGA configuration files are stored. The DOM (Disk-on-Module)
holds the system software and test data. The RTC is a real-time clock. The system
configurations are stored in the EEPROM. VRAM is the memory for video display. See Figure
2-9.

2-23
 System Discription

Figure 2-9 Working principles of the CPU board

„ Basic Functions

To receive analog signals as WBC/RBC/PLT counts, HGB measurement, aperture voltage

and vacuum/pressure signals, etc.

To monitor such system parameters as the +56V, +12V and -12V supplies of the analog
board, the +3.3V and +12V supplies of the CPU board itself and the temperature of the
analyzer.

To receive the keypad signal and control the keypad buzzer and LCD backlight.

To generate control signals to control the valves, pumps, aperture zapping, HGB LED, current
source and digital pot.

To drive/light the LCD and adjust the contrast.

To drive the keypad, printer and floppy drive.

Analog Board
„ Working Principles

The analog board converts the signals output from each sensor into required digital signals;
provides control/drive and monitoring signals.

„ Basic Functions

The analog board includes 6 functional units: interface unit, power supply unit (DC-DC),
power supply monitoring unit, volumetric signal unit, HGB unit and vacuum/pressure unit.

2-24
 System Discription

Drive Board
„ Working Principles

The drive board drives the valves, pumps and motors. It carries out the following instructions
sent by the CPU: to open/close the pumps and solenoid valves; to control the syringe motors;
to control the movement of the probe; and to hold the motor in the standby mode.

„ Basic Functions

The drive board drives the valves, pumps and motors. It carries out the following instructions
sent by the CPU: to open/close the pumps and solenoid valves; to control the syringe motors;
to control the movement of the probe; and to hold the motor in the standby mode.

Power block

The power block includes the 5V, 12V and 30V DC supplies. The 12V powers the valve/pump
and rotation motor drive circuits (max. current: 2A). The 30V powers the drive circuits of
elevator motors and 10mL&50uL&2.5mL syringe motors (max. current: 4A). The 5V powers
the drive chips of valve/pump and rotation motors (max. current: 50mA) and the chip control
system (max. current: 200mA).

Switch control block

The switch control block consists of the photo-coupling circuit and valve/pump drive circuit, as
shown in Figure 2-10.

Figure 2-10 Switch control block

„ Photo-coupling Circuit

The photo-coupling circuit mainly consists of the photo-coupler and resistors. It provides 20
TTL outputs to the valves and pumps. The photo-coupler, TLP521-2, isolates the digital
ground from the driving ground.

„ Valve/Pump Drive Circuit

The drive circuit controls the valves and pumps by using the digital signals from the main
control board. The technical parameters include the following:
Number of channels: 20 (2 pumps and 18 valves);
Output: TTL, active low.
Power: 12V/1A (max.)
The input is isolated from the output (the digital ground is isolated from the power ground).

2-25
 System Discription

According to the drive voltage and max drive current for pumps/valves, the ULN2068B chip,
which can drive 1.5A current, is applied to drive the pumps and valves.

Motor control block

The motor control block is composed of the serial communication circuit, probe control/drive
circuit, syringe motor control/drive circuit, position sensor drive/detection circuit.

„ Serial Communication Circuit

Since the CPU board requires a 3.3V power supply while the drive board requires a 5V power
supply, a photo-coupler (H11L1) is needed for the purposes of conversion and isolation.

„ Probe Control/Drive Circuit

The probe control/drive circuit includes the elevator/rotation motor control/drive circuits. The
control system of the probe motor consists of an AT89S52 MCU and ADM705 WDT. The
AT89S52 also detects signals coming from the position sensors and manages the serial
ports.

2.3.2 Volumetric Unit

The volumetric unit is located above the vacuum chamber assembly. The upper end of the
metering tube is connected to the solenoid valves by T-pieces, while the lower end to the
vacuum chamber by tubes. The metering tube itself is fixed on the volumetric unit by 2
brackets. Together with the metering tube, the pot on the metering tube can be adjusted to
ensure correct level signals.

„ Working Principles

The volumetric unit measures the volume of the samples during counting and outputs the
start and end signals of counting. The principle diagram of the volumetric unit is shown in
Figure 2-11.

Figure 2-11 Principle diagram of the volumetric unit

2-26
 System Discription

The volumetric unit consists of a sensor, consistent-current circuit and output circuit.

2.3.3 Power Supply Assembly

As shown in Figure 2-12, the power supply assembly consists of the power board, filter,
equipotentiality terminal, etc.

Filter

Power board

Equipotentiality
terminal

Figure 2-12 Power supply assembly

2.3.4 Panel Assembly

The panel assembly consists of such user interfaces as recorder (recorder drive board),
keypad, indicator board, screen unit (LCD, inverter and LCD adapter), as shown in Figure
2-13 and Figure 2-14.

Indicator board

Keypad board

Figure 2-13 Panel disassembly view (1)

2-27
 System Discription

LCD Inverter

LCD adapter

Recorder

Figure 2-14 Panel disassembly view (2)

The serial signal lines, +5V and +12V power cords, the 5V ground line and the power ground
are directed from the same connector of the CPU board. They are connected to the front
panel by one cable and then split respectively to the recorder and the keypad. The LCD
signal line is isolated. The inverter, powered by keypad power supply, drives the backlight of
LCD. The backlight brightness can be adjusted via the keypad. An LCD adapter is added
here.

Keypad
„ Working Principles

The keypad adapter mainly consists of an MCU, keypad matrix, backlight/power indicator
control and buzzer, as shown in Figure 2-15.

Figure 2-15 Hardware structure of the keypad

„ Basic Functions

To scan the keypad: The keypad adapter scans the keypad and reports the scanned key

2-28
 System Discription

code to the main control board.

To control the LCD brightness: The keypad adapter receives instructions from the main
control board to turn on/off the backlight and power indicators of the LCD and to adjust the
brightness of the backlight.

To control the buzzer: The keypad adapter receives instructions from the main control board
to turn on/off the buzzer.

LCD Adapter
„ Basic Functions

The LCD adapter connects the LCD to the CPU board, as shown in Figure 2-16.

Figure 2-16 LCD adapter connection

2-29
 System Discription

2.4 Software Introduction

The software system includes the boot software and system software. The boot software,
loaded automatically by the hardware, runs in the FLASH. It configures the FPGA, drives the
keypad, turns on the LCD backlight, loads the system software, and finally transfers the CPU
control to the system software. The system software, loaded by the boot software, runs in the
SDRAM. It gets resource data from the external DOM, and provides such functions as
counting, system setup, quality control, maintenance, etc. The system software sends
instructions to corresponding fluidic unit at given time according to the basic timing.

2.4.1 Menu Tree

The system menu includes 9 options. The option with a “ ” has submenus. The expanded
menu is shown in Figure 2-17 .

2-30
 System Discription

Figure 2-17 Menu tree

2.4.2 Password

There are three levels of password: user, administrator and service engineer. Authorities for

2-31
 System Discription
each level are described in Table 2-1.
Table 2-1 Password level and its authorities
If the … Is at …
User Count
Sample Mode
Sample Table Review
Sample Histogram Review
L-J AnalysisÆL-J Edit
L-J AnalysisÆL-J Count
L-J AnalysisÆL-J Graph
“L-J AnalysisÆL-J Table
X-B AnalysisÆLimit
X-B AnalysisÆSamples/Batch
X-B AnalysisÆStart/Stop
X-B AnalysisÆ X-B Graph
X-B AnalysisÆX-B Table
SetupÆPrint
SetupÆCount Time
SetupÆPassword
SetupÆRef. Range
SetupÆTransmission
SetupÆDate & Time
SetupÆ Gain
SetupÆAuto Clean Time
SetupÆReagent Exp. Date
SetupÆReport Title
SetupÆParameter Units
SetupÆOther Settings
ServiceÆMaintenance
ServiceÆSystem Status
ServiceÆValve Test
ServiceÆSystem Test
2-32
He/She can …
Start analysis.
Switch between Whole Blood and Predilute.
Go to/find/select/print/transmit/search
sample result. Selecting the sample results,
press [7] to view the reproducibility.
Go to/browse/print sample result.
View L-J settings.
Run controls.
View L-J graph.
View L-J table.
View X-B settings.
View number of samples for each X-B
analysis.
View availability of X-B analysis.
View X-B graph.
View X-B table.
Set print format, printer and auto print.
View count time of WBC and RBC.
Change the password.
View reference ranges of general
parameters.
Modify the transmission settings.
Set the system date and time.
View the gains of WBC(Whole Blood),
WBC(Predilute), RBC, HGB.
Set the auto clean time.
Set the expiration date of diluent, rinse and
lyse.
Set the title of report to be printed.
View the current units of parameters
Set the contrast of LCD.
Set alarm time for error messages.
Set reminder of predilute mode.
Maintain the analyzer.
View the system status.
Check the valves.
Check the circuits, mechanics and fluidic
components of the system.
 System Discription
ServiceÆPrepare to Ship
ServiceÆError Message
CalibrationÆManual
Help
Shutdown
Administrator Count
Sample Histogram Review
Sample Table Review
System Test
Calibrator
Manual
SetupÆGain
SetupÆCount Time
SetupÆParameter Units
SetupÆRef. Range
L-J AnalysisÆL-J Edit
L-J AnalysisÆL-J Count
L-J AnalysisÆL-J Table
X-B AnalysisÆLimit
X-B AnalysisÆSamples/Batch
X-B AnalysisÆStart/Stop
X-B AnalysisÆX-B Table
Service Count
Engineer
Sample Histogram Review
Sample Table Review
SetupÆOther Settings
2-33
Prepare the analyzer for a prolonged period
of non-use or for shipping.
View the error messages.
View the manual calibration factors.
View the help document.
Shut down the analyzer.
Adjust the sample histogram.
Adjust the sample histogram.
Delete sample results when deletion is
enabled.
Adjust the motors
Perform auto calibration
Calculate the calibration factors manually.
Modify the gain for WBC whole blood, WBC
predilute, RBC and HGB.
Modify the count time of WBC and RBC.
Modify the parameter units.
Modify the reference ranges of general
parameters.
Edit L-J settings, delete parameter limits and
QC data.
Run controls and delete QC results.
View and delete L-J table.
Edit X-B settings, delete parameter limits
and X-B data.
Change the number of samples for each X-B
analysis.
Enable/Disable X-B analysis.
View and delete X-B table.
Press [1], [2], [3] to view the sampling pulses
for WBC, RBC and PLT respectively.
Press [↑] to upgrade the system software.
Press [F5] to view special sample
information.
Delete sample results.
Enable/Disable sample result deletion.
Change the language.
Change the WBC and RBC metering tube
settings.
Modify the serial No. of the analyzer.
Modify the threshold for probe cleanser
cleaning.
Press [DEL] to view the configurations.
 System Discription

Press [DEL] to view the configurations of the

analyzer. Press [F1] to export the
configurations.
Error Message Press [DEL] to delete the error messages.
System Test Enter the elevator motor test screen. Press
[ASPIRATE] to adjust probe settings.
Enter the rotation motor test screen. Press
[ASPIRATE] to adjust rotation motor
settings.
Maintenance Check the lyse.
Empty the baths.
SetupÆGain View the PLT gain.

2-34
 3 Updating the Software

3.1 Preparation for Update

1. Update program comes from the installation CD. The CD contains many folders, which
also include a number of subfolders. Structure of the CD is shown in Table 3-1. Note that
the actual CD may be different from this one;

Table 3-1 Structure of the installation CD

Folder Description Subfolder Comment

1#_LOGO LOGO Startup LOGO 3005-30-44798 English
2#_FPGA FPGA configuration 3005-30-34947 /
file
3#_SEQUENCE Timing library 3006-30-74803 /
4#_CONFIG Software 3006-30-74800 English
configurations
5#_BIOS Boot software 3005-30-35024 /
6#_LANGUAGE Language library 3006-30-74801 English
7#_SYSTEM System software 3006-30-74799 /

2. There are two classes of files for updating: information file (UPDATE.CFG) and data file.
They can be put in one or more floppy disks;

3. Only one module can be upgraded at one time. Update disks of different modules must
not be used simultaneously, since some files in the update disks have the same name
which would lead to update failure, i.e. all the information files use the same filename
“Update.cfg”.

3.2 Making Update Floppy Disk

The upgrading files come from the installation CD.

1. Prepare one or more formatted floppy disks (1.44MB);

2. Start up the PC;

3. Insert the installation CD into the CD-ROM;

4. Find the subfolder where the desired upgrading file locates. Copy all information files and
data files in the subfolder to the floppy disk, and then cancel the Read Only property of all
files.

3-1
 Updating the Software

3.3 Update Procedure

1. The analyzer enters the “Count” screen after startup;

2. Ensure printing and data transmission is finished;

3. Select “SetupÆPassword”. Enter the password of service engineer;

4. At the “Count” screen, press [↑] (up arrow). The following prompt is displayed;

“Insert disk of config, then press [ENTER] key.”

5. Insert the floppy disk with information files into the floppy drive, press [ENTER].The
following prompt is displayed;

“Reading config file ...”

6. After the analyzer reads and analyzes the upgrading files, the following prompt is
displayed;

“Insert disk of data. Then press [ENTER] key.”

7. Insert the floppy disk with data files into the floppy drive, press [ENTER]. The system
writes the data files to the temporary folder of the DOM;

8. When the system reads the files successfully, the following prompt is displayed.

“Writing files to disk or flash ...”;

9. After the files are written to the working folder of the DOM and the temporary folder is
cleared, the following prompt is displayed;

“Update succeeded. Waiting for restarting ...”

10. Few seconds later, the analyzer is restarted. The update is finished.

3.4 Precautions
1. During update, the operator must not go away and should watch each prompt displayed;

2. During update, the analyzer must not be powered off;

3. During update, if “Printing. Try again later.” is displayed, don’t operate until the printing
is finished;

4. During update, if the following prompts repeat;

“Insert disk of config, then press [ENTER] key.” or “Insert disk of data, then press
[ENTER] key.”

5. Check if you insert the correct disk. If the disk reading fails 3 times, the analyzer returns
to the “Count” screen;

6. If “Updating failed” is displayed, the analyzer will return to the “Count” screen in few
seconds. If the system software can not be normally loaded in later use, you can only
restore it by reinstalling the DOM.

3-2
 4 Repair Procedures

4.1 Introduction

Refer to section 4.2 - 4.13 for detailed procedures. The service engineer must verify the
repair strictly as instructed by the enclosed steps, if any.

4-1
 Repair Procedures

4.2 Disassembling the Panels

4.2.1 Left Door

„ Purpose

The left door needs to be opened when you replace lyse or service the analyzer.

„ Opening

1. Shut down the analyzer and disconnect it from the power supply;

2. Push the black latch afterwards, as shown in Figure 4-1;

3. While pushing the latch, rotate the left door in the direction indicated in Figure 4-1.

Figure 4-1 Opening the left door

1--- Black latch 2--- Left door

„ Installation

Install the left door according to the steps above in reverse order.

„ Verification

Move the left door and check if it is fixed.

4.2.2 Right Door

4-2
 Repair Procedures

„ Purpose

The right door needs to be opened when you replace the fluidic components or open the front
door.

„ Opening

1. Shut down the analyzer and disconnect it from the power supply;

2. Push the black latch afterwards, as shown in Figure 4-2;

3. While pushing the latch, rotate the right door in the direction indicated in Figure 4-2.

Figure 4-2 Opening the right door

1--- Black latch 2--- Right door

„ Installation

Install the right door according to the steps above in reverse order.

„ Verification

Move the left door and check if it is fixed.

4.2.3 Front Door

„ Purpose

The front door needs to be opened when you replace the probe, bath, LCD and recorder.

„ Opening

1. Shut down the analyzer and disconnect it from the power supply;

4-3
 Repair Procedures

2. Restore the sample compartment to working position;

3. Open the right door as instructed by section 4.2.2;

4. Pull upwards the latch on the front sheet metal, as shown in Figure 4-3;

5. Rotate the front door in the direction indicated in Figure 4-3.

z Close the sample compartment when opening the front door, otherwise you
may destroy them.

Figure 4-3 Opening the front door

1--- Sample compartment 2--- Left door

3--- Latch 4--- Pin
5--- Front door

„ Installation

1. Close the front door;

4-4
 Repair Procedures

2. Press down the latch;

3. Close the right door.

„ Verification

1. Move the front door and check if it is fixed;

2. Power on the analyzer and check if it can start up normally.

4.2.4 Top Cover

„ Purpose

The top cover needs to be removed when you replace the analog board, drive board, power
supply assembly, connecting cables and tubes, etc.

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the three M4X8 screws with the screwdriver, as instructed by Figure 4-4;

3. Pull the top cover backwards and then remove it, as instructed by Figure 4-4.

Figure 4-4 Removing the top cover

4-5
 Repair Procedures

1--- Top cover 2--- Front sheet metal

3--- M4X8 screw

„ Installation

Install the top cover according to the steps above in reverse order.

„ Verification

Check if the top cover and the screws are fixed.

4.2.5 Back Cover

„ Purpose

The back cover needs to be removed when you replace the boards, cables and fluidic
components.

„ Tool

2# cross screwdriver

„ Removal

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Remove the power supply assembly as instructed by section 4.4;

4. Open the left door as instructed by section 4.2.1;

5. Unscrew the five M3X4 screws fixing the lyse support and then remove the support, as
shown in Figure 4-5;

6. Unplug the tubes from the diluent inlet, rinse inlet and waste outlet. Unplug the diluent
and rinse sensor connectors;

7. Unscrew the eight M4X8 screws and remove the back cover (together with the left and
right doors, as shown in Figure 4-5).

z Reagents and waste are potentially infectious. Wear proper personal

protective equipment (e.g. gloves, lab coat, etc.) and follow safe laboratory
procedures when handling them in the laboratory.

4-6
 Repair Procedures

z The reagents are irritating to eyes, skin and diaphragm. Wear proper
personal protective equipment (e.g. gloves, lab coat, etc.) and follow safe
laboratory procedures when handling them in the laboratory.

z Be sure to dispose of reagents, waste, samples, consumables, etc.

according to government regulations.

z If the reagents accidentally spill on your skin, wash them off with plenty of
water and if necessary, go see a doctor; if the reagents accidentally spill into
your eyes, wash them off with plenty of water and immediately go see a
doctor.

Figure 4-5 Removing the back cover

1--- Lyse support 2--- M3X4 screws

3--- Lyse inlet 4--- Rinse inlet
5--- Diluent sensor connector 6--- Rinse sensor connector
7--- Waste outlet 8--- Back cover (together with the left and
right doors)

„ Installation

Install the back cover according to the steps above in reverse order.

4-7
 Repair Procedures

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4-8
 Repair Procedures

4.3 Replacing the Boards

z When maintaining the electronic parts of the analyzer, be sure to remove the
metallic decorations you are wearing so as to avoid damage to you and the
analyzer.

z Static discharging may damage the electronic parts, be sure to wear

antistatic hand rings while maintaining or perform maintenance on the
antistatic platform.

The analog board, CPU board and drive board are fixed on the mounting plate, as shown in
Figure 4-6.

Figure 4-6 Boards layout

1--- Analog board 2--- Drive board

3--- CPU board 4--- Pressure chamber assembly

4.3.1 Analog Board

„ Purpose

The analog board collects original signals and provides control/drive and monitoring signals.
It needs to be replaced when going wrong.

4-9
 Repair Procedures

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Unscrew the #6-32X6 knurled screw fixing the shielding box and then remove the box
cover, as shown in Figure 4-7;

4. Unplug the J1-J5 connectors and the two tubes from the analog board;

5. Unscrew the four M3X8 screws and remove the analog board, as shown in Figure 4-7.

Figure 4-7 Removing the analog board

1--- Shielding box 2--- # 6-32X6 knurled screw

3--- Tube 4--- M3X8 screw

„ Installation

4-10
 Repair Procedures

Install the analog board according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. Re-adjust the gains and conduct repeatability test as instructed by section 4.13.

4.3.2 CPU Board

„ Purpose

The CPU board, the key part of the analyzer, receives signals and monitors/drives peripheral
devices. It needs to be replaced when going wrong.

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Unplug the J1, J3, J4, J5, J6, J7, J8, J10, J11 and J16 connectors from the CPU board,
as shown in Figure 4-8;

4. Remove the DOM from the CPU board, as shown in Figure 4-8;

5. Unscrew the four M3X8 screws and remove the CPU board, as shown in Figure 4-8.

4-11
 Repair Procedures

Figure 4-8 Removing the CPU board

1--- M3X8 screw 2--- CPU board

„ Installation

Install the CPU board according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4.3.3 Drive Board

„ Purpose

The drive board drives the valves, pumps and motors. It needs to be replaced when going
wrong.

4-12
 Repair Procedures

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Unplug the J1-J10 connectors from the drive board, as shown in Figure 4-9;

4. Unscrew the four M3X8 screws and remove the drive board, as shown in Figure 4-9.

Figure 4-9 Removing the drive board

1--- Drive board 2--- M3X8 screw

„ Installation

Install the drive board according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

4-13
 Repair Procedures

4.3.4 Keypad
„ Purpose

The keypad needs to be replaced when going wrong.

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Open the front door as instructed by section 4.2.3;

3. Unscrew the six M3X8 screws fixing the LCD, as shown in Figure 4-10;

4. Unplug the J2, J3 connectors from the keypad and remove the LCD;

5. Unscrew the seven M3X8 screws fixing the keypad and then remove the keypad, as
shown in Figure 4-10.

4-14
 Repair Procedures

Figure 4-10 Removing the keypad

1--- Font door 2--- M3X8 screw

3--- LCD 4--- Keypad
5--- M3X8 screw

„ Installation

Install the keypad according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ

4-15
 Repair Procedures

fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4.3.5 Indicator Board

„ Purpose

The indicator board needs to be replaced when going wrong.

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Open the front door as instructed by section 4.2.3;

3. Unplug the J1, J2 connectors from the indicator board, as shown in Figure 4-11;

4. Unscrew the four M3X8 screws and then remove the indicator board, as shown in Figure
4-11;

5. Remove the key caps from the keys on the indicator board.

4-16
 Repair Procedures

Figure 4-11 Removing the indicator board

1--- Front door 2--- M3X8 screw

3--- Indicator board

„ Installation

Install the indicator board according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4-17
 Repair Procedures

4.4 Replacing the Power Supply Assembly

„ Purpose

The power supply assembly needs to be replaced when it goes wrong or when you remove
the back cover.

„ Tool

2# cross screwdriver.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Unplug the J1, J11 and J7 from the analog board, CPU board and drive board
respectively, as shown in Figure 4-12;

4. Unscrew the four M4X8 screws and then remove the power supply assembly in the
direction indicated in Figure 4-12.

Figure 4-12 Removing the power supply assembly

1--- Power supply assembly 2--- M4X8 screw

4-18
 Repair Procedures

„ Installation

Install the power supply assembly according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4-19
 Repair Procedures

4.5 Replacing the LCD

„ Purpose

The LCD needs to be replaced when going wrong.

„ Tool

1#, 2# cross screwdrivers.

„ Removal

The LCD is removed in two procedures.

Procedure 1: To remove the LCD from the front door.

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Open the front door as instructed by section 4.2.3;

4. Remove the back cover as instructed by section 4.2.5;

5. Unplug the J8 connector from the CPU board, and unscrew the M3X8 screw fixing the
LCD ground wire, as shown in Figure 4-13;

6. Unscrew the six M3X8 screws fixing the LCD to the front door, as shown in Figure 4-13;

7. Unplug the J2, J3 connectors from the keypad and then remove the LCD in the direction
indicated in Figure 4-13.

4-20
 Repair Procedures

Figure 4-13 Removing the LCD

1--- Black latch 2--- M3X8 screw

3--- Front door 4--- M3X8 screw
5--- LCD

Procedure 2: To further disassemble the LCD, as Figure 4-14 Disassembling the LCD shows.

4-21
 Repair Procedures

Figure 4-14 Disassembling the LCD

1--- LCD mounting plate 2--- Hexagon nut M2

3--- Cross counter sunk screw M2X8 4--- Insulation plate
5--- Inverter 6--- Inverter shield
7--- LCD 8--- Washer
9--- Cross pan head screw M2X8 10--- FPC cable
11--- LCD adapter 12--- M3X8 screw
13--- Shielding box 14--- Clasp
15--- M3X8 screw 16--- LCD connecting cable

„ Installation

Install the LCD according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4-22
 Repair Procedures

4.6 Replacing the Recorder

„ Purpose

The recorder needs to be replaced when going wrong.

„ Tool

1#, 2# cross screwdrivers.

„ Removal

The recorder is removed in two procedures.

Procedure 1: To remove the recorder from the front door, as shown in Figure 4-15.

1. Shut down the analyzer and disconnect it from the power supply;

2. Press the bulge of the recorder door;

3. Rotate the recorder door in the direction indicated in Figure 4-15;

4. Unscrew the two M3X12 screws fixing the recorder to the shielding box;

5. Open the front door as instructed by section 4.2.2;

6. Unscrew the four M3X8 screws fixing the shielding box to the front door;

7. Unplug the JP1 and JP3 connectors from the recorder drive board;

8. Squeeze the bulges on the left/right sides of the recorder and then remove the recorder
in the direction indicated in Figure 4-15.

4-23
 Repair Procedures

Figure 4-15 Removing the recorder

1--- Bulge of the recorder door 2--- Recorder door

3--- M3X12 screw 4--- Shielding box
5--- Bulges on left/right sides of the recorder

Procedure 2: To further disassemble the recorder, as shown in Figure 4-16.

1. Toggle the bulge on the recorder box, remove the drive board and unplug the circuit
board connected to it. Replace the drive board if it goes wrong;

2. Press the bulge of the recorder door;

3. Rotate the recorder door in the direction indicated in Figure 4-16;

4. Toggle upwards the paper tension lever on the thermal head. Remove the paper, if any;

5. Unscrew the two PT2X6 screws fixing the thermal head and then remove the thermal
head.

4-24
 Repair Procedures

Figure 4-16 Disassembling the recorder

1--- Bulge 2--- Circuit board

3--- Recorder drive board 4--- Bulge of the recorder door
5--- Paper tension lever 6--- Thermal head
7--- PT2X6 screw

„ Installation

Install the recorder according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization.

4-25
 Repair Procedures

4.7 Replacing the Fluidic Components

The fluidic components, which perform tasks according to the given sequencing, include:
solenoid valve, syringe, volumetric metering tube, pump, vacuum chamber and pressure
chamber, as shown in Figure 4-17. They need to be maintained or replaced when going
wrong.

Figure 4-17 Fluidic components layout

1--- Vacuum chamber 2--- Metering tube

3---SV8 4---SV18
5---SV7 6---SV17
7---SV16 8---SV15
9---SV5 10---SV1
11---SV6 12---SV3
13---SV4 14---Syringes assembly
15---SV9 16---SV2
17---SV10 18---SV11
19---SV12 20---SV14

4-26
 Repair Procedures

21---SV13

4.7.1 Valves
„ Purpose

The solenoid valves control the fluid flow or direct it to different tubes. Many kinds of fluid may
flow through the valves during analysis. The valves will probably go wrong when getting
blocked or eroded.

„ Tool

2# cross screwdriver.

„ Removal

There are 18 valves on the BC-3200. SV5 is taken for example.

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Open the right door as instructed by section 4.2.2;

4. Unplug the connector on the back of the SV5, as shown in Figure 4-18;

5. Unplug the tubes connecting the SV5 to the SV4 and bath, as shown in Figure 4-18;

6. Unscrew the two M3X8 screws fixing SV5 and then remove the SV5, as shown in Figure
4-18.

4-27
 Repair Procedures

Figure 4-18 Removing the valves

1--- Connector 2--- Tube

3--- SV5 4--- M3X8 screw

„ Installation

Install the valve according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware
Æfluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. Click [MENU] on the “Count” screen. The system menu is displayed. SELECT
“ServiceÆValve Test” to enter the “Valve Test” screen. SELECT a valve to check the
valve you’ve selected.

4-28
 Repair Procedures

4.7.2 Pumps
„ Purpose

The pumps, consisting of the pneumatic pump, fluid pump and supports, drive the whole
system during analysis. They need to be maintained or replaced when going wrong.

„ Tool

Diagonal pliers, 2# cross screwdriver

„ Removal

The pumps are removed in two procedures.

Procedure 1: To remove the pumps from the support.

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Open the left door as instructed by section 4.2.1;

3. Unscrew the five M3X4 screws fixing the lyse support and then remove the support, as
shown in Figure 4-19;

4. As shown in Figure 4-19, unplug the tubes connected to the air vent of the pneumatic
pump and to the inlet/outlet of the fluid pump. Unplug the connectors from the two pumps.
Remove the two 82－32UNC-2B nuts;

5. Unscrew the M4X8 screws fixing the pumps and then remove the pumps, as shown in
Figure 4-19.

4-29
 Repair Procedures

Figure 4-19 Removing the pumps

1--- M3X4 screw 2--- Lyse support

3--- Connector 4--- Pumps
5--- Connector 6--- Tube
7--- 82－32UNC-2B nut 8--- M4X8 screw

Procedure 2: To further disassemble the pumps.

Usually, only the fluid pump and pneumatic pump need to be replaced.

„ Disassembling the fluid pump

Unscrew the four M3X8 screws fixing the fluid pump and then remove the pump, as shown in
Figure 4-20

4-30
 Repair Procedures

Figure 4-20 Disassembling the fluid pump

1--- Fluid pump 2--- M3X8 screw

„ Disassembling the pneumatic pump

Cut the belts binding the pneumatic pump to the support and then remove the pump, as
shown in Figure 4-21.

Figure 4-21 Disassembling the pneumatic pump

1--- Pneumatic pump

„ Installation

Install the pumps according to the steps above in reverse order.

4-31
 Repair Procedures

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. The status of the vacuum chamber and the pressure chamber can indicate how the two
pumps are going;

4. Press [MENU] at the “Count” screen. The system menu is displayed. SELECT “Service
Æ System Test” to enter the “System Test” screen. SELECT “Vacuum” to check the fluid
pump; or SELECT “PC Pressure” to check the pneumatic pump.

4.7.3 Syringes
„ Purpose

Syringes aspirate specified volume of fluid. They often need to be replaced when going
wrong.

„ Tool

2# cross screwdriver, slotted screwdriver, 2mm hexagon wrench.

„ Removal

The syringes are removed in two procedures.

Procedure 1: To remove the syringes from the support.

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Open the right door as instructed by section 4.2.2;

4. Unplug the connectors of position sensors, motors and cables, as shown in Figure 4-22.

5. Unplug the tubes connecting the syringes to the SV6, SV4, SV3 and the probe, as shown
in Figure 4-22;

6. Unscrew the four M4X8 screws fixing the syringes to the support and then remove the
syringes, as shown in Figure 4-22.

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 Repair Procedures

Figure 4-22 Removing the syringes

1--- Position sensor connector 2--- Motor connector

3--- Tube 4--- SV6
5--- SV4 6--- SV3
7--- Syringes 8--- M4X8 screw

Procedure 2: To further disassemble the syringes.

The fragile parts include the 2.5 mL / 10mL, 50µL syringes, two motors and position sensors.

1. Removing the 2.5mL / 50µL syringes, as shown in Figure 4-23;

2. The two syringes are removed in completely the same way. The 50µL one is taken for
example;

3. Unscrew the three M3X12 screws and then remove the presser bar;

4. Unscrew the screws fixing the plunger rod and then remove the 50µL syringe.

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 Repair Procedures

Figure 4-23 Removing the 2.5 mL and 50µL syringes

1--- Presser bar 2--- M3X12 screw and washer

3---50µL syringe 4--- Screw

„ Removing the 10ml syringe, as shown in Figure 4-24.

1. Unscrew the two M3X12 screws and then remove the presser bar;

2. Unscrew the screw fixing the plunger rod and then remove the 10mL syringe.

Figure 4-24 Removing the 10mL syringe

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 Repair Procedures

1--- Presser bar 2--- M3X12 screw and washer

3---10mL syringe 4--- Screw

„ Removing the motors, as shown in Figure 4-25.

The two motors are removed in completely the same way.

1. Loosen or remove the screws fixing the lead screw;

2. Unscrew the two M3X8 screws fixing the motor and then remove the motor.

Figure 4-25 Removing the motors

1--- Presser bar 2--- M3X12 screw and washer

3---10mL syringe 4--- Screw

„ Removing the position sensors

Unscrew the two M3X8 screws fixing the photo-coupler switch and then remove the sensors,
as shown in Figure 4-26.

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 Repair Procedures

Figure 4-26 Removing the position sensors

1--- M3X8 screw 2--- Photo-coupler switch

„ Installation

Install the syringes according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. If hardware test is finished without error, fluidic system test starts. If the syringes go
normally, the analyzer will run normally, otherwise the fluidic system test is skipped and the
analyzer directly enters the “Count” screen.

4.7.4 Vacuum Chamber

„ Purpose

The vacuum chamber stores compressed air to drive the fluid flow and also stores the air
when the analyzer flushes the aperture, therefore it may be broken in some cases because it
always suffers from external pressure.

„ Tool

2# cross screwdriver.

„ Removal

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 Repair Procedures

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Open the right door as instructed by section 4.2.2;

3. Unplug the tubes connecting the vacuum chamber to the metering tube and SV10, as
shown in Figure 4-27;

4. Unscrew the two M3X8 screws fixing the vacuum chamber and then remove the vacuum
chamber, as shown in Figure 4-27.

Figure 4-27 Removing the vacuum chamber

1--- Right door 2--- Tube

3--- Vacuum chamber 4--- Tube
5--- M3X8 screw

„ Installation

Install the vacuum chamber according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of systemÆhardwareÆ

fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. Press [MENU] at the “Count” screen. The system menu is displayed. SELECT “Service”
Æ”System Test” to enter the “System Test” screen. SELECT “Vacuum” to check the fluid

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 Repair Procedures

pump.

4.7.5 Pressure Chamber

„ Purpose

The pressure chamber stores the air to drive the air flow inside the analyzer. It may be broken
in some cases because it always suffers from external pressure.

„ Tool

2# cross screwdriver.

„ Removal

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Open the left door as instructed by section 4.2.1;

3. Unscrew the five M3X4 screws fixing the lyse support and then remove the support, as
shown in Figure 4-28;

4. Unplug the tubes connecting the pressure chamber to the pneumatic pump, the analog
board and the bath, as shown in Figure 4-28;

5. Unscrew the M3X8 screws fixing the pressure chamber and then remove the pressure
chamber, as shown in Figure 4-28.

Figure 4-28 Removing the preesure chamber

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 Repair Procedures

1--- Left door 2--- Lyse support

3--- M3X4 screw 4--- Pressure chamber
5--- Tube 6--- M3X8 screw

„ Installation

Install the pressure chamber according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware
Æfluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. Press [MENU] at the “Count” screen. The system menu is displayed. SELECT “Service
Æ System Test” to enter the “System Test” screen. SELECT “PC Pressure” to check the
pneumatic pump.

4.7.6 Metering Tube

„ Purpose

The metering tube measures the volume of the samples during counting. It may bring
erroneous results if foreign matters exist in it. The failure of the photo-coupler sensor may
also affect the analysis.

„ Tool

2# cross screwdriver.

„ Removal

The metering tube is removed in two procedures.

Procedure 1: To remove the metering tube from the support, as shown in Figure 4-29.

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Open the right door as instructed by section 4.2.2;

4. Unplug the J1 connector from the back of the volumetric unit;

5. Unscrew the two M3X8 screws fixing the shielding box and then remove the box;

6. Unplug the tubes connecting the metering tube to the vacuum chamber, the SV7, SV8,
SV17 and SV18;

7. Unscrew the two M3X8 screws and the two M3X12 screws and then remove the

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 Repair Procedures

metering tube.

Figure 4-29 Removing the metering tube

1--- J1 connector 2--- Shielding box

3--- M3X8 screw 4--- Tube
5--- Tube 6--- SV17
7--- SV7 8--- SV18
9--- SV8 10--- M3X8 screw
11--- M3X12 screw

Procedure 2: To further disassemble the metering tube, as shown in Figure 4-30.

Only the volumetric unit and the metering tube need to be replaced during maintenance.

1. Separate the volumetric unit, the metering tube and other parts;

2. Remove the metering tube with rubber sleeves from the holder;

3. Remove the rubber sleeves from the metering tube.

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 Repair Procedures

Figure 4-30 Disassembling the metering tube

1--- Volumetric unit 2--- Holder

3--- Rubber sleeve 4--- Metering tube (with T-piece connector)

„ Installation

Install the metering tube according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. After the analyzer enters the “Count” screen, remove the shielding box over the
metering tube and start background counting. Check the fluid flow in the tube and the count
time. The analyzer returns to the “Count” screen after counting if the photo-coupler works
normally.

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 Repair Procedures

4.8 Replacing the Bath Assembly

The bath assembly consists of the WBC/RBC units, isolation chamber and shielding box. The
parts to be replaced include the HGB unit/aperture of the WBC unit and aperture of the RBC
unit.

4.8.1 HGB Unit and Aperture of the WBC Unit

„ Purpose

The HGB unit, installed on the WBC bath, measures the Hemoglobin Concentration of
samples. It may lead to erroneous results when installed incorrectly.

The aperture is the key part of analyzer. It may often be blocked due to its small inner
diameter.

„ Tool

1#, 2# cross screwdriver.

„ Removal

The HGB unit and aperture of the WBC unit are removed in three procedures.
Procedure 1: To remove the WBC unit from the analyzer, as shown in Figure 4-31.

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Open the right door as instructed by section 4.2.2;

4. Open the front door as instructed by section 4.2.3;

5. Unplug the WBC connector (J5) from inside the shielding box of the analog board as
instructed by section 4.3.1;

6. Unscrew the two M3X6 screws fixing the outer WBC shielding box and then remove it in
the direction indicated in Figure 4-31;

7. Loosen the two M3X6 screws fixing the WBC shielding box and then remove it in the
direction indicated in Figure 4-31;

8. Unplug the tubes connecting the WBC unit to the SV8, the shielding plate and the
isolation chamber;

9. Unplug the WBC unit manually.

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 Repair Procedures

Figure 4-31 Removing the WBC unit

1---Outer WBC shielding box 2---M3X6 screw with washer

3---M3X6 screw with washer 4---WBC shielding box
5---Tube 6---Tube
7---Tube 8---WBC unit

Procedure 2: To remove the HGB unit from the WBC unit, as shown in Figure 4-32
Unscrew the M2X10 screws and then remove the HGB unit from the font/back bath.

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 Repair Procedures

Figure 4-32 Removing the HGB unit

1---M2X10 screw 2---Font/back bath

3---Right bracket 4---Left bracket

Procedure 3: To remove the aperture from the WBC unit, as shown in Figure 4-33.
Unscrew the two M3X10 screws and then remove the aperture from the front/back bath.

Figure 4-33 Removing the aperture of the WBC bath

1--- Aperture 2--- Front bath

3--- M3X10 screw

„ Installation

Install the HGB unit and aperture according to the steps above in reverse order.

4-44
 Repair Procedures

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. When the analyzer enters the “Count” screen, open the front door and check if blue light
exists in the WBC bath. If it does, the HGB unit is properly connected;

4. Start background counting on the “Count” screen. If the aperture works normally, the
WBC counting time is within the normal range, otherwise bubbles will appear or the
aperture will be blocked.

4.8.2 Aperture of the RBC Unit

„ Purpose

The aperture is the key part of analyzer. It may often be blocked due to its small inner
diameter.

„ Tool

1#, 2# cross screwdriver.

„ Removal

The aperture of the RBC unit is removed in two procedures.

Procedure 1: To remove the RBC unit from the analyzer, as shown in Figure 4-34

1. Start up the system and empty the tubes. Shut down it and disconnect it from the power
supply;

2. Remove the top cover as instructed by section 4.2.4;

3. Open the right door as instructed by section 4.2.2;

4. Open the front door as instructed by section 4.2.3;

5. Unplug the RBC connector (J4) from inside the shielding box of the analog board as
instructed by section 4.3.1;

6. Unscrew the two M3X6 screws fixing the outer RBC shielding box and then remove it in
the direction indicated in Figure 4-34;

7. Loosen the two M3X6 screws fixing the RBC shielding box and then remove it in the
direction indicated in Figure 4-34;

8. Unplug the tubes connecting the RBC unit to the SV8, the shielding plate and the
isolation chamber. Unscrew the M3X8 screw fixing the ground wire of the RBC unit;

9. Unplug the RBC unit manually;

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 Repair Procedures

Figure 4-34 Removing the RBC unit

1--- Outer RBC shielding box 2--- M3X6 screw

3--- RBC shielding box 4--- M3X6 screw
5--- M3X8 screw 6--- Ground wire
7--- Tube 8--- Tube
9--- Tube 10--- RBC unit

Procedure 2: To remove the aperture from the RBC unit, as shown in Figure 4-35

Unscrew the two M3X10 screws and then remove the aperture from the front/back baths.

4-46
 Repair Procedures

Figure 4-35 Removing the aperture of the RBC bath

1--- Aperture 2--- Front bath

3--- M3X10 screw

„ Installation

Install the aperture according to the steps above in reverse order.

„ Verification

1. Check if all parts are fixed in place;

2. Power on the analyzer. It starts initialization test in the order of system Æ hardware Æ
fluidic system. If the cables and tubes are connected normally, the analyzer enters the
“Count” screen without alarm after initialization;

3. Start background counting on the “Count” screen. If the aperture works normally, the
RBC counting time is within the normal range, otherwise bubbles will appear or the
aperture will be blocked.

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 Repair Procedures

4.9 Replacing the Probe Assembly

4.9.1 Probe Assembly
„ Purpose

The probe assembly can be removed or replaced as instructed by this section.

„ Tool

2# cross screwdriver, 2.5mm hex wrench, 150mm monkey wrench and diagonal pliers.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4. Open the right door and front door
as instructed by section 4.2.2./ 4.2.3;

3. Remove the probe from the probe assembly, as shown in Figure 4-36;

a) Unplug the tube from the probe.

b) Unscrew the hexagon screw fixing the probe.
c) Hold the upper end of the probe and then remove it upwards.

Figure 4-36 Removing the probe

1 --- Probe 2 --- Hexagon screw

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 Repair Procedures

z The probe tip is very sharp. Be sure to treat it carefully to avoid personal
injuries during analysis or maintenance.

z The probe tip is sharp and may contain biohazardous materials. Exercise
caution to avoid contact with the probe when working around it.

4. Remove the probe wipe from the probe assembly, as shown in Figure 4-37;

a) Unplug the clip;

b) Separate the two tubes on the probe wipe from the tube clamp and then remove the
probe wipe in the direction indicated in Figure 4-37.

Figure 4-37 Removing the probe wipe

1 --- Clip 2 --- Probe wipe

3 --- Lower rotation plate 4 --- Tube clamp
5 --- Tube

5. Unplug the 4 sensor connectors;

6. Unplug the 2 motor sensors;

7. Unscrew the 3 socket head screws fixing the sample compartment and then hold and put

4-49
 Repair Procedures

it on the platform, as shown in Figure 4-38;

Figure 4-38 Removing the sample compartment

8. Remember the probe tube layout and then remove the belts binding the tube on the
bracket;

9. Unscrew the three M4 screws fixing the probe assembly and then remove it slowly, as
shown in Figure 4-39.

Figure 4-39 Removing the probe assembly

1 --- Probe tube 2 --- Probe assembly

4-50
 Repair Procedures

„ Installation

1. Install the probe assembly to the corresponding place on the front plate;

2. Tighten the three M4 screws to fix the probe assembly;

3. Install the sample compartment on the probe assembly and tighten the three socket
head screws;

4. Connect the motor connectors (MOTOR Y/C34937-A for upper motor and MOTOR
X/C34936-A for lower motor);

5. Connect the sensor connectors;

a) The external sensor on the top left corner of the probe assembly indicates “XL” and
internal one indicates “XR”;
b) The upper sensor on the right of the probe assembly indicates “YU” and the lower one
indicates “YD”.

6. Install the probe wipe in the aligning hole on the lower rotation plate, insert the clip and
direct the two tubes in the tube clamp;

7. Install the probe;

a) Pull the guide block of the probe assembly upward to the upper position;
b) Insert the probe down into the aligning hole. When the tip reaches to the probe wipe,
rotate the probe to have its tip against the outlet of the probe wipe;
c) Fix the probe with a hexagon screw by using a monkey wrench.

8. Direct the probe tube according to its original layout and fix it with belts. Connect the tube
to the upper end of the probe.

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 Repair Procedures

4.10 Rotation Sensor

„ Purpose

How to replace the rotation sensor is described.

„ Tool

2# cross screwdriver and 2.5mm hex wrench.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4. Open the right door and front door
as instructed by section 4.2.2./4.2.3;

3. Unscrew the screw fixing the rotation sensor, as shown in Figure 4-40;

4. Remove the rotation sensor assembly;

5. Unplug the connector of the sensor to be replaced;

6. Unscrew the screw fixing the to-be-replaced sensor to the mounting plate.

Figure 4-40 Removing the rotation sensor

1 --- Fluidic plate 2 --- Front plate

3 --- Screw fixing the rotation sensor 4 --- Mounting plate
5 --- XL 6 --- XR

4-52
 Repair Procedures

„ Installation

To install a new rotation sensor, refer to steps 1-6 above.

4.10.1 Elevator Sensor

„ Purpose

How to replace the elevator sensor is described.

„ Tool

2# cross screwdriver and 2.5mm hex wrench.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Open the right door and front door as instructed by section 4.2.2./4.2.3;

3. Unscrew the two M3 screws fixing the elevator motor, as shown in Figure 4-41;

4. Remove the elevator motor assembly on the front plate from the right of the analyzer;

5. Unplug the connector of the sensor to be replaced;

6. Unscrew the screw fixing the to-be-replaced sensor to the mounting plate.

Figure 4-41 Removing the elevator sensor

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 Repair Procedures

1 --- Screw fixing the elevator sensor 2 --- Front plate

3 --- YU 4 --- Front plate
5 --- YD

„ Installation

To install a new elevator sensor, refer to steps 1-6 above.

4.10.2 Disassembling the Probe Assembly

„ Purpose

You can refer to this section when removing or replacing the entire probe assembly or its
special parts.

„ Tool

2# cross screwdriver, 2.5mm hex wrench, 1.5mm hex wrench, 150mm monkey wrench,
slotted head screwdriver and diagonal pliers.

„ Removal

Remove the entire probe assembly as instructed by section 4.9.1.

Elevator motor and reduction synchronous belt

1. Unscrew the four M3X8 screws fixing the elevator motor;

2. Separate the elevator motor assembly from the probe assembly and then remove the
reduction synchronous belt;

3. Unscrew the four M3X6 screws on the elevator motor assembly and then remove the
motor;

4. Loosen the two M3X5 (Figure 4-42) screws between the synchronous belt pulley and the
rotor, and then remove the synchronous belt pulley.

4-54
 Repair Procedures

Figure 4-42 Removing the elevator motor and the reduction synchronous belt

1 --- Elevator motor 2 --- Motor support

3 --- Active synchronous belt pulley 4 --- M3X5 screw
5 --- Reduction synchronous belt

Synchronous belt and lower belt pulley

1. Unscrew the two M3X8 screws fixing the synchronous belt on the guide block assembly
and then remove the clamping plate;

2. Press the middle of the belt for several times till sense its tension, as shown in Figure
4-43;

4-55
 Repair Procedures

Figure 4-43 Sensing the tension of the synchronous belt

3. Loosen the two M3X5 screws (Figure 4-44) on the lower belt pulley;

4. Unscrew the two M3X8 screws fixing the synchronous belt and then remove it from the
belt pulleys;

5. Take out theφ4slotted washer and then remove the belt roller from the 2 flanged
bearings.

4-56
 Repair Procedures

Figure 4-44 Removing the synchronous belt and the lower belt pulley

1 --- Clamping plate 2 --- Synchronous belt

3 --- M3X5 screw 4 --- Belt pulley
5 --- Belt roller 6 --- Flanged bearing
7 --- Bearing support 8 --- φ4 slotted washer

Reduction synchronous belt pulley assembly

1. Unscrew the two M3X8 screws and then remove the reduction synchronous belt pulley
assembly;

2. Loosen the two M3X5 screws on the bigger belt pulley and then remove the belt pulley;

3. Loosen the two M3X5 screws on the smaller belt pulley and then remove the belt pulley;

4. Take out the φ4 slotted washer and then remove the belt roller (Figure 4-45) from the 2
flanged bearings.

4-57
 Repair Procedures

Figure 4-45 Removing the reduction synchronous belt pulley assembly

1 ---φ4 slotted washer 2 --- Reduction synchronous belt pulley

3 --- M3X5 screw 4 --- Flanged bearing
5 --- Bearing support 6 --- Belt roller
7 --- Synchronous belt pulley

Guide block assembly and rotation motor

1. Unscrew the socket head screw and then remove the rotation sensor, as shown in Figure
4-46;

2. Unscrew the M3X6 screw fixing the tube bracket and then remove the bracket, as shown
in Figure 4-46;

3. Unscrew the two M3X5 screws fixing the rotation motor;

4. Unscrew the 4 socket head screws fixing the rotation motor and then remove the motor,
guide block assembly and the flanged bearing, as shown in Figure 4-46;

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 Repair Procedures

Figure 4-46 Removing the guide block assembly and the rotation motor

1 --- Socket head screw 2 --- Rotation sensor

3 --- Tube bracket 4 --- M3X6 screw
5 --- Flanged bearing 6 --- Guide block assembly
7 --- M3X5 screw 8 --- Rotation motor

5. Loosen the M3X5 screw (Figure 4-47) on the control sleeve and then remove the sleeve;

6. Unscrew the M3X6 screw fixing the induction plate and then remove the plate;

7. Hold the guide rod, unscrew the M3X8 screw fixing the rod and then remove the rod;

8. Unscrew the two M3X5 screws fixing the guide rotor and then remove the lower rotation
plate;

9. Remove the guide block assembly from the guide rotor;

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 Repair Procedures

10. Unscrew the four M3X16 screws on the guide block assembly.

Figure 4-47 Disassembling the block assembly

1 --- Control sleeve 2 --- M3X5 screw

3 --- M3X6 screw 4 --- Induction plate
5 --- Guide rod 6 --- Linear bearing
7 --- Probe support 8 --- Guide sleeve
9 --- Guide block assembly 10 --- M3X5 screw
11 --- Lower rotation plate

Linear guide pair

1. Unscrew the four M3X8 screws on the upper/lower blocks (Figure 4-48) and then remove
the two blocks;

2. Unscrew the 4 socket head screws fixing the linear guide pair and then remove the guide
pair.

Note: When removing the guide pair, ensure the block on the guide pair does not exceed its

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 Repair Procedures

two ends.

Figure 4-48 Removing the linear guide pair

1 --- Upper/Lower blocks 2 --- Linear guide pair

„ Installation

To install the linear guide pair and the upper/lower blocks

1. Install the guide pair in the corresponding slot on the mounting plate, and then tighten the
four M3X8 screws;

2. Install the upper/lower blocks tightly on the block of the guide pair, and then tighten the
four M3X8 screws.

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 Repair Procedures

Figure 4-49 Installing the upper/lower block

To install the guide block assembly and rotation motor

1. Put the probe support on the screw side of the guide sleeve, with the screw thread side
upward. String them with the linear bearing and then tighten the four M3X16 screws;

2. Insert the guide rotor into the guide block assembly, with the linear bearing side upward;

3. Install the lower rotation plate on the guide rotor. Insert the guide rod through the upper
rotation plate, probe support and then the lower rotation plate. Then install the M3X6 screw
on the upper end of the guide rod;

4. Put the guide block assembly on the corner of a flat table or on a pad, as shown inFigure
4-50, with the guide rod and guide rotor close to the table or pad. Then tighten the two
M3X5 screws to fix the rotor of the rotation motor;

5. Do the remaining installation according to steps 1-6 in section Guide block assembly
and rotation motor.

Figure 4-50 Installing the guide block assembly

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 Repair Procedures

To install the reduction synchronous belt pulley assembly:

Install the reduction synchronous belt pulley assembly according to steps 1- 4 in section
Reduction synchronous belt pulley assembly.

To install the synchronous belt and lower belt pulley assembly:

Install the synchronous belt and lower belt pulley assembly according to steps 1- 5 in section
Synchronous belt and lower belt pulley assembly.

To sense the tension of the synchronous belt:

1. First loosely install the two M3X8 screws to fix the synchronous belt assembly, as shown
in Figure 4-51;

2. Adjust the tension of the belt with the adjusting screw;

3. Press the middle of the belt in the direction indicated in Figure 4-51 till sense the same
tension as before removing;

4. Then tighten the two M3X8 screws.

Figure 4-51 Sensing the tension of the synchronous belt

1 --- Tension adjuster 2 --- Adjusting screw

3 --- Synchronous belt 4 --- M3X8 screw

5. To install the elevator motor and reduction synchronous belt:

6. Install the elevator motor and reduction synchronous belt according to steps 1- 4 in
section Elevator motor and reduction synchronous belt.

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 Repair Procedures

z The reduction synchronous belt shall be tensed to the extent that it is just
straightened.

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 Repair Procedures

4.11 Replacing the Sample Compartment Assembly

4.11.1 Sample Compartment Assembly and Door

„ Purpose

The sample compartment assembly can be removed or replaced as instructed by this

section.

„ Tool

2.5mm hex wrench.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4. Open the right door and front door
as instructed by section 4.2.2./4.2.3;

3. Unplug the 5 connectors from the top of the analyzer: K6, K5, K1, K3 and K7;

4. Unscrew the 3 socket head screws fixing the sample compartment and then take out the
sample compartment.

Figure 4-52 Removing the sample compartment

z Exercise caution on the cable connectors when taking out the sample
compartment.

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 Repair Procedures

„ Installation

A new sample compartment can be installed according to the above-mentioned steps 1-4.

Note: Connect the upper switch of tube position to K6 and the lower switch to K5.

„ Position correction

Adjust the compartment door according to the panel opening, as shown in Figure 4-53.
Loosen the retaining screw and adjust the compartment door in the arrow direction. Ensure
the space between one side of the compartment door and the panel is about 1.5mm.

Figure 4-53 Adjusting the compartment door position

1 --- Retaining screw 2 --- Sample compartment door

3 --- Front door

4.11.2 Electromagnet Assembly

„ Purpose

How to replace the electromagnet and other parts is described.

„ Tool

2# cross screwdriver and nipper pliers.

„ Removal

1. Shut down the analyzer and disconnect it from the power supply;

2. Remove the top cover as instructed by section 4.2.4. Open the right door and front door

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 Repair Procedures

as instructed by section 4.2.2./4.2.3;

3. Unplug the connector marked “K7” from the top of the analyzer;

4. Unscrew the 3 retaining screws fixing the electromagnet assembly, as shown in Figure
4-54;

Figure 4-54 Disassembling the electromagnet assembly 1

1 --- Sample compartment assembly 2 --- Electromagnet assembly

3 --- Retaining screw

5. Take out the φ2 slotted washer with nipper pliers and then remove the spring, as shown
in Figure 4-55;

Figure 4-55 Disassembling the electromagnet assembly 2

1 --- Spring 2 ---φ2 slotted washer

6. Unscrew the retaining screws to remove the electromagnet, support, paper washer and
magnetic core, as shown in Figure 4-56;

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 Repair Procedures

Figure 4-56 Disassembling the electromagnet assembly 3

1 --- Retaining screw 2 --- Support

3 --- Magnetic core 4 --- Paper washer
5 --- Electromagnet

7. Remove other parts such as washer, cotter pin and fitting pin, as shown in Figure 4-57.

Figure 4-57 Disassembling the electromagnet assembly 3

1 --- Washer 2 --- Cotter pin

3 --- Fitting pin

„ Installation

A new electromagnet assembly can be installed according to the above-mentioned steps 1-7.

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 Repair Procedures

4.11.3 Disassembling the Sample Compartment Assembly

„ Purpose

You can refer to this section when removing or replacing the entire sample compartment
assembly or its special parts.

„ Tool

2# cross screwdriver, 2.5/1.5/3mm hex wrench, nipper pliers, slotted head screwdriver and
diagonal pliers.

„ Removal

Remove the entire sample compartment assembly as instructed by section 4.10.1.

Switching elements
1. Cut the belts on the switch cable support, as shown in Figure 4-58;

Figure 4-58 Cutting the belts

1 --- Support 2 --- Belt

3 --- Switch cable

2. Remove the switching elements, as shown in Figure 4-59.

4-69
 Repair Procedures

Figure 4-59 Removing the switching elements

1 --- M3X4 screw 2 --- K1 switch

3 --- K6 switch 4 --- Switch plate
5 --- K5 switch 6 --- Retaining screw
7 --- K3 switch

Gear and revolution axis

1. Unscrew the two M3X6 screws on the gear and then remove the gear;

2. Loosen the M3X5 screw on the switch inductor and then remove the inductor;

3. Loosen the two M3X5 screws on the gear and then remove the gear and bush;

4. Loosen the two M3X5 screws on the tube holder. Remove the slotted washer on the
electromagnet side and then remove the revolution axis;

5. Remove the tube holder assembly.

4-70
 Repair Procedures

Figure 4-60 Removing the gear and revolution axis

1 --- M3X6 screw 2 --- φ3 washer

3 --- Gear 4 --- Switch inductor
5 --- M3X5 screw 6 --- Gear
7 --- Bush 8 --- Tube holder
9 --- Revolution axis 10 --- Slotted washer

Tube holder assembly

1. Unscrew the two M5X6 screws on the tube holder assembly and then remove the 2
springs and φ4 steel balls, as shown in Figure 4-61;

4-71
 Repair Procedures

Figure 4-61 Removing the steel balls

1 ---φ4 steel ball 2 --- Spring

3 --- M5X6 screw

2. Unscrew the M4X20 (Figure 4-62) screw in the center of the rotation body, and then
remove the tube compartment from the rotation body;

Figure 4-62 Removing the tube compartment

1 --- Tube compartment 2 --- Tube holder

3 --- Rotation body 4 --- M4X20 screw

3. Unscrew the 2 retaining screws fixing the lock plate and then remove the lock plate.

4-72
 Repair Procedures

Figure 4-63 Removing the lock plate

1 --- Tube holder 2 --- M3X6 screw

3 --- Lock plate

„ Installation

To install the tube holder:

Install the tube holder assembly according to the above-mentioned steps 1-3.
Note: When tightening the two M5X6 screws on the tube holder, ensure the edge of the screw
is level to or 0.3mm lower than the surface of the tube holder.

To install the gear and revolution axis:

Install the gear and revolution axis according to the above-mentioned steps 1-5.
Note: Before tightening the two M3X5 screws on the tube holder, adjust the tube holder
according to the support position and make the tube holder in the middle of the two
supporting plates with a spacer of 2mm. Then tighten the 2 screws on the surface
corresponding to the revolution axis.

4-73
 Repair Procedures

Figure 4-64 Installing the tube holder

1 --- Supporting plate 2 --- Support

3 --- Revolution axis 4 --- Tube holder assembly
5 --- M3X5 screw

To install the switching elements:

Install the switching elements according to the above-mentioned steps 1-2.

4-74
 Repair Procedures

4.12 Adjusting the Sample Probe Position

4.12.1 Start Position

„ Purpose

To completely wash the exterior of the lower probe end, the probe position shall be adjusted
according to the probe wipe. The probe assembly is designed to have a fixed start position.
Since error may exist, the pre-sample position of the probe needs to be calibrated before the
analyzer leaves the factory, and users don’t need to calibrate it again. Service engineers
should readjust the above-mentioned two positions of the probe when replacing the DOM,
probe assembly, sample compartment assembly, drive board and CPU board.

„ Adjustment

For password level of service engineer or above, press [MENU] to enter the “System Test”
screen, then move the cursor to “Elevator motor”, press [ENTER] and then [ASPIRATE].
The “Adjust sample probe position” window, as shown in Figure 4-65, is displayed. Move
the dotted line frame to “Start Position” and then press [ENTER]. The selected option
appears in black. Press [↑] and [↓] to adjust the probe tip position, ensuring it is level to the
inlet of the probe wipe. When the probe tip is in required position, press [ENTER] to save the
adjustment.

Figure 4-65 Adjust sample probe position window

4.12.2 Pre-sample Position

„ Purpose

Error may exist when the sample probe is installed. To better aspirate samples, the
pre-sample position of the probe needs to be calibrated before the analyzer leaves the factory,
and users don’t need to calibrate it again. Service engineers should readjust the start position
and pre-sample position of the probe when replacing the DOM, probe assembly, sample
compartment assembly, drive board and CPU board.

4-75
 Repair Procedures

„ Adjustment

Adjustment of the pre-sample position is generally the same with the start position. However,
in the “Adjust sample probe position” window, move the dotted line frame to “Pre-sample
Position” and then press [ENTER]. The selected option appears in black. Press [↑] and [↓] to
adjust the probe tip position, ensuring it is aligned to the surface of the tube holder. When the
probe tip is in required position, press [ENTER] to save the adjustment.

Note: Sometimes 1step forward or backward is ideal for the probe tip. In this case, either of
the 2 steps can be set to be the home position.

„ Verification

Check whether the sample probe stops in the given position of the probe wipe without alarm.
If it does, the adjustment is successful, otherwise readjust it.

z Be sure to close the compartment door when adjusting the sample probe
position.

z The probe tip is sharp and may contain biohazardous materials. Exercise
caution to avoid contact with the probe when working around it.

4-76
 Repair Procedures

4.13 Adjusting the Rotation Position of the Sample

Probe

„ Purpose

Due to assembly error, the sample probe probably can not move to the center of each hole.
Therefore, the three positions (right, middle and left) need to be calibrated before the
analyzer leaves the factory, and users don’t need to calibrate them again. Service engineers
should readjust the start position and pre-sample position of the probe when replacing the
DOM, probe assembly, sample compartment assembly, drive board and CPU board.

„ Adjustment

For password level of service engineer or above, press [MENU] to enter the “System Test”
screen, then move the cursor to “Rotation motor”, press [ENTER] and then [ASPIRATE].
The “Rotation adjustment” window, as shown in Figure 4-66 is displayed. Move the dotted
line frame to “Middle Position” and then press [ENTER]. The selected option appears in
black. Check whether the sample probe is in the center of the tube by selecting “Elevator
motor”. Press [←] and [→] to adjust the probe position. When it is in the center of the tube,
press [ENTER] to save the adjustment.

Enter the “Rotation adjustment” window to move one step forward and backward, save the
result and exit to the “System Test” screen. Check whether alarms occur when the sample
probe is in the two positions. If no alarm occurs, the position is acceptable. Enter the
“Rotation adjustment” window again to reset the home position. The home position and
critical status confirmation procedure of the left/right position of the probe is similar to that of
the middle position, except that the sample probe should be in the middle of the WBC bath or
the RBC bath.

Figure 4-66 Rotation adjustment window

4-77
 Repair Procedures

„ Verification

If no alarm regarding rotation motor error occurs, the adjustment is successful, otherwise
readjust the rotation position.

z The probe tip is sharp and may contain biohazardous materials. Exercise
caution to avoid contact with the probe when working around it.

4-78
 Repair Procedures

4.14 Adjusting the Gain

„ Purpose

To get accurate test results, the gain shall be adjusted again after replacement of the analog
board, aperture or the entire bath, DOM, and after long use of the analyzer.

„ Adjustment

Adjusting the gain of the WBC channel

1. Enter the service engineer password to access to the “Setup” Æ “Gain” screen, as
shown in Figure 4-67;

Figure 4-67 Gain screen

2. To set the WBC gain with EDTA-K2 venous blood: Run the anticoagulant venous blood in
whole blood mode. Adjust the WBC (whole blood) gain to make most sample histograms
end at 300-350. At least 5 normal samples shall be used;

3. To set the WBC gain with capillary blood: Run the diluted capillary blood. Adjust the WBC
(predilute) gain to make most sample histograms end at 300-350. At least 5 normal
samples shall be used.

Adjusting the gain of the RBC channel

1. Enter the service engineer password to access to the “Setup Æ Gain” screen, as shown
in Figure 4-67;

2. Run a normal control two times on the “Count” screen and take the average of the two

4-79
 Repair Procedures

MCV measurements as the final MCV result;

3. Calculate the adjustment rate for the RBC channel:

Assume the expected MCV result is 90.0fL and the obtained MCV result is 82.0fL.
Then

ExpectedMCV 90.0
× 100%＝ × 100% = 109.8%
ActualMCV 82.0

4. Adjust the RBC gain according to the calculated rate above.

Adjusting the gain of the HGB channel

1. Enter the service engineer password to access to the “Setup Æ Gain” screen, as shown
in Figure 4-67;

2. Adjust the HGB gain to make the blank voltage of HGB about 4.5V.

„ Verification

1. Run several normal samples in whole blood and predilute modes and then check if the
WBC sample histograms end at 300-350;

2. Run controls on the “Count” screen and check if the MCV result is close to the reference
value;

3. Enter the “Service Æ System Status” screen, check if the blank voltage of HGB is about
4.5V.

4-80
 5 Troubleshooting

5.1 Error Codes

Code Error Code Error Code Error

0x0401 Ambient Temp. 0x0402 Background 0x0403 HGB Error
Abnormal Abnormal
0x0404 HGB Adjust 0x0405 WBC Clog 0x0406 WBC Bubbles
0x0407 RBC Clog 0x0408 RBC Bubbles 0x4011 Waste Full
0x0801 Com. Error 0x0802 Barcode Error 0x0803 Barcode Com Error
0x1001 Printer out of paper 0x1002 Printer Offline 0x1003 Recorder Com
error
0x1004 Recorder out of 0x1005 Recorder too Hot 0x1006 Press Bar Up
Paper
0x2001 Lyse Empty 0x2002 Diluent Expiry 0x2003 Rinse Expiry
0x2004 Lyse Expiry 0x2005 Vacuum Filter 0x2006 Real-time Clock
Error Error
0x4002 10mL Syringe 0x4001 2.5mL and 50µL 0x4004 Elevator Motor
Motor Error Syringe Motor Error
Error
0x4003 Rotation Motor 0x4008 DC12V Power 0x400A 5V Power Error
Error Error
0x4009 3.3V Power Error 0x400B 56V Power Error 0x4006 RBC Interrupt Error
0x4005 WBC Interrupt Error 0x4007 PLT Interrupt 0x400D PC Pressure Low
Error
0x400C Vacuum Low 0x400E VC Pressure Low 0x400F Diluent Empty
0x4010 Rinse Empty 0x8001 File Error 0x8002 Dynamic Memory
Error
0x4012 Front Cover Open 0x2007 Sample 0x2008 Sample Comp.
Compartment Door Open
Error
0x2009 Deviated Tube / / / /
Position

5-1
 Troubleshooting

5.2 Troubleshooting

Do the following procedures to troubleshooting the analyzer.

1. According to the indicated errors, find the corresponding error in the table below;

2. Remove the errors according to the probable causes and recommended action.

5.2.1 Pressure errors

Error Message Description Possible Cause(s)

PC Pressure The pressure inside 1. Pressure pump error;
Low the pressure chamber
2. Air leakage from the fluidic and/or pressure
does not reach the
chamber;
expected value within
the given time. 3. Poor connection of the pressure transducer;

4. Clogged filter connecting the pressure pump.

Vacuum Low The vacuum degree 1. Fluidic pump error;

does not reach the
2. Air leakage from the fluidic and/or vacuum
expected value within
chamber;
the given time.
3. Poor connection of the vacuum transducer;

4. Damaged V10 valve connecting the vacuum

chamber;

5. Unsmooth discharge of waste.

VC Pressure The pressure inside 1. Air leakage from the fluidic and/or vacuum
Low the vacuum chamber chamber;
does not reach the
2. Poor connection of the vacuum transducer;
expected value within
the given time. 3. Pressure pump error;

4. Damaged V9 valve connecting the pressure

chamber and the vacuum chamber.

Vacuum Filter The air inside the 1. Clogged vacuum filter;

Error vacuum chamber is
2. Malfunction of the V17 valve and V7 valve
not extracted within
that connecting with the filter.
the given time.

5-2
 Troubleshooting

5.2.2 Reagent errors

Error Message Description Possible Cause(s)

Lyse Empty No lyse is detected by 1. The lyse has run out;
the level transducer.
2. The transducer is poorly connected or
transducer is damaged. Transducer needs
changing.

Diluent Empty No diluent is detected 1. The diluent has run out;

by the level
2. The transducer is poorly connected or
transducer.
transducer is damaged. Transducer needs
changing.

Rinse Empty No rinse is detected 1. The rinse has run out;

by the level
2. The transducer is poorly connected or
transducer.
transducer is damaged. Transducer needs
changing.

5.2.3 Hardware errors

Error Message Description Possible Cause(s)

Real-time Clock The time displayed is 1. Someone tempered with the on-board battery
Error wrong. off the board;

2. Something is wrong with the on-battery (poor

contact, dead battery, etc.);

3. Damaged real-clock chip;

4. If not, enter “Setup → Date & Time” screen

and reset the time as instructed by Chapter 5.7.
Restart the analyzer after the adjustment and
the time should be correct.

10mL Syringe The motor does not 1. Pressed or blocked tubes;

Motor Error reach the expected
2. Poor contact of the drive line;
position.
3. Abnormal motor;

4. Poor connection between the drive board and

the CUP board;

5. Malfunctioning or poor connecting

5-3
 Troubleshooting

photocoupler;

6. Damaged syringe or the resistance of the

syringe too large;

7. Syringe installed improperly;

8. If not, enter the “Service → System Test”

screen and check the motor. The error will be
removed if the test result is normal.

2.5mL & 50µL The motor does not 1. Pressed or blocked tubes;
Syringe Motor reach the expected
2. Poor contact of the drive line;
Error position.
3. Abnormal motor;

4. Poor connection between the drive board and

the CUP board;

5. Malfunctioning or poor connecting

photocoupler;

6. Damaged syringe or the resistance of the

syringe is too large;

7. Syringes installed improperly;

8. If not, enter the “Service → System Test”

screen and check the motor. The error will be
removed if the test result is normal.

Elevator Motor The elevator motor 1. Jammed sample probe;

Error does not reach the
2. Poor contact of the drive line;
expected position.
3. Abnormal motor;

4. Poor connection between the drive board and

the CUP board;

5. Malfunctioning or poor connecting

photocoupler;

6. Motor installed improperly;

7. If not, enter the “Service → System Test”

screen and check the motor. The error will be
removed if the test result is normal.

Rotation Motor The rotation motor 1. Jammed sample probe;

Error does not reach the
2. Poor contact of the drive line;
expected position.
3. Abnormal motor;

4. Poor connection between the drive board and

the CUP board;

5-4
 Troubleshooting

5. Malfunctioning or poor connecting

photocoupler;

6. Motor installed improperly;

7. Too tight of the tube connecting the probe

wipe;

8. Improperly fixing of the tube connecting the

sample probe;

9. If not, enter the “Service → System Test”

screen and check the motor. The error will be
removed if the test result is normal.

WBC Interrupt Something is wrong 1. Enter the “Service → System Test” screen
Error with the A/D part of and check the WBC AD interrupt;
the CPU board.
2. The error will be removed if the test result is
normal.

RBC Interrupt Something is wrong 1. Enter the “Service → System Test” screen
Error with the A/D part of and check the RBC AD interrupt.
the CPU board.
2. The error will be removed if the test result is
normal.

PLT Interrupt Something is wrong 1. Enter the “Service → System Test” screen
error with the A/D part of and check the PLT AD interrupt;
the CPU board.
2. The error will be removed if the test result is
normal.

Deviated Tube The tube position 1. Open the sample compartment door and
Position where a sampling readjust the sample position;
shall run deviates
2. The error will be removed if the adjustment is
from sample position.
successful. Or, readjust again.

Sample Comp. Sample compartment 1. Close the sample compartment door;

Door Open door is at open status.
2. Check the switch of the sample compartment
door and the connecting line;

3. If the problem remains, enter the “Service →

System Test” screen and check the sample
compartment door status;

4. The error will be removed if the test result is

“OFF”.

Sample Sample compartment 1. Enter the “Service → System Test” screen

Compartment door cannot open. and check the sample compartment door status;
Error
2. The error will be removed, if the test result is

5-5
 Troubleshooting

normal and the sample compartment door can

open.

Front Cover The front cover is at 1. Close the front cover;

Open open status.
2. If the problem remains, enter the “Service →
System Test” screen and check the status of
front cover;

3. The error will be removed if the test result is

“OFF”.

5.2.4 Power supply errors

Error Message Description Possible Cause(s)

DC 12V Power Something is wrong Something is wrong with the power supply of the
Error with the DC 12V circuit board. Enter the “Service” → “System
power supply. Status” screen and record the “DC 12V” and “DC
-12V” values.
5V Power Error Something is wrong Something is wrong with the power supply board
with the 5V power and the analog board. Enter the “Service →
supply. System Status” screen and record the “5V”
voltage.
3.3V Power Something is wrong It may be caused by the 5V power supply or the
Error with the 5V power CPU board error. Enter the “Service → System
supply. Status” screen and record the “3.3V” voltage.
56V Power Error Something is wrong Something is wrong with the DC-DC converter or
with the 56V voltage. the consistent current source. Enter the “Service
→ System Status” screen and record the “56V”
voltage.

5.2.5 Measurement errors

Error Message Description Possible Cause(s)
Background 1. The background 1. Contaminated bath(s) or aperture(s);
Abnormal is outside the 2. The diluent is contaminated or expired;
specification range
3. Bubbles or clog is flagged during the
or “*****” is
background counting;
displayed.
4. Interference from the outside occurs for the
2. Data length of
shielding is not close enough.
background pulse
channel increases
obviously.

5-6
 Troubleshooting

HGB Error HGB blank voltage 1. No liquid in the bath during counting;
within 0 V - 3.2 V or
2. Reagents expired or contaminated.
4.9 V - 5 V.
HGB Adjust HGB blank voltage 1. HGB light installed improperly;
within 3.2 V - 3.4 V or
2. HGB light strength set improperly;
4.8 V - 4.9 V.
3. Dirty WBC bath;

4. Reagents expired or contaminated.

WBC Clog WBC counting for too 1. WBC channel reference count time set
long or no counting incorrectly;

2. Dirty bath or aperture;

3. Damaged photocoupler on the metering tube;

4. Something is wrong with V8 valve;

5. Abnormal vacuum in the vacuum chamber.

WBC Bubbles WBC counting for too 1. WBC channel reference count time set
short incorrectly;

2. Reagents connected incorrectly. Diluent

running along the metering tube side;

3. Something is wrong with the photocoupler on

the metering tube;

4. Something is wrong with V7 valve and the

fluid in the metering tube cannot be well
emptied;

5. The drive board connected improperly.

RBC Clog RBC counting for too 1. RBC channel reference count time set
long or no counting incorrectly;

2. Dirty bath or aperture;

3. Damaged photocoupler on the metering tube;

4. Something is wrong with V8 valve;

5. Abnormal vacuum in the vacuum chamber.

RBC Bubbles RBC counting for too 1. RBC channel reference count time set
short incorrectly;

2. Reagents connected incorrectly;

3. Diluent running along the metering tube side;

4. Something is wrong with the photocoupler on

the metering tube;

5. Something is wrong with V7 valve and the

5-7
 Troubleshooting

fluid in the metering tube cannot be well

emptied;

6. The drive board connected improperly.

5.2.6 External connection errors

Error Message Description Possible Cause(s)
Com Error Something is wrong 1. Communication cable not well connected;
with the
2. Inappropriate communication settings.
communication
between the external
computer and the
analyzer.
Barcode Com Something is wrong 1. Poor connection between the scanner and the
Error with the analyzer;
communication
2. Scanner not specified is used.
between the scanner
and the analyzer.
Barcode Error Something is wrong 1. Poor connection between the scanner and the
with the barcode analyzer;
scanning.
2. Scanner not specified is used;

3. Too long barcode or invalid barcode.

Printer Offline Something is wrong 1. The printer is not turned on;

with the connection
2. Poor connection between the printer and the
between printer and
analyzer;
the analyzer.
3. Printer not specified is used.

Recorder Com Something is wrong 1. Poor connection between the recorder and
Error with the the CPU board;
communication
2. Damaged recorder.
between the recorder
and the CPU board.
Printer out of Something is wrong Printer paper running out or not properly installed.
Paper with the installation of
the printer paper.
Recorder out of Something is wrong Something is wrong with the installation of the
Paper with the installation of recorder paper.
the recorder paper.
Recorder too Something is wrong 1. The recorder has been running for a too long
Hot with the recorder. period;

2. The recorder needs cleaning;

5-8
 Troubleshooting

3. Damaged recorder.

Press Bar Up Something is wrong 1. Tension lever not replaced;

with the recorder
2. Damaged recorder.
installation.

5.2.7 Ambient temperature error

Error Message Description Possible Cause(s)

Ambient Temp. The ambient 1. Abnormal ambient temperature or
Abnormal temperature detected temperature transducer error;
is out of the expected
2. Enter the “Service → System Status” screen
range.
to check the ambient temperature;

3. If the actual ambient temperature exceeds the

pre-defined ambient temperature, adjust the
temperature. Otherwise, the analysis results
may be unreliable.

5.2.8 Other errors

Error Message Description Possible Cause(s)
File Error Something is wrong An error occurred when saving files. Restart the
with the system analyzer. If the error remains, re-plug the DOM
software or the DOM. and start the analyzer again. If the error still
remains, change the DOM or the CPU board.
Dynamic Something is wrong Something is wrong with the dynamic memory.
Memory Error with the system Turn off the analyzer, check the connector or
software or the DOM. change the CPU board, then restart the analyzer.

5-9
 Troubleshooting

5.3 Using Pulse Graphs to Troubleshoot the

Analyzer

Abnormal pulse graph will be generated if some parts of the analyzer go wrong. Since the
analyzer can store the original pulse graphs automatically, checking the abnormal pulse
graphs helps to troubleshoot the analyzer.

To check the pulse graphs, you enter the password “3210” and press [ENTER] key to exit.
Pressing the [1], [2] and [3] respectively at the “Count” screen, the original pulse graphs of
WBC, RBC and PLT channels appear. Do not use the channel data length to troubleshoot the
analyzer.

5.3.1 Normal pulse graph

Figure 5-1 WBC pulse graph of normal Figure 5-2 Pulse graph of normal WBC
sample background

Figure 5-3 RBC pulse graph of normal Figure 5-4 Pulse graph of normal RBC
sample background

5-10
 Troubleshooting

Figure 5-5 PLT pulse graph of normal Figure 5-6 Pulse graph of normal PLT
sample background

5.3.2 Abnormal pulse graph

To check the pulse graphs, you enter the password “3210” and press [ENTER] key to exit.
Pressing the [1], [2] and [3] respectively at the “Count” screen, the original pulse graphs of
WBC, RBC and PLT channels appear. Do not use the channel data length to troubleshoot the
analyzer.

Figure 5-7 Severe interference in WBC Figure 5-8 Severe interference in WBC
channel (Background) channel (Normal sample)

Figure 5-9 Severe interference in RBC Figure 5-10 Severe interference in

channel(Background) RBC channel (Normal sample)

5-11
 Troubleshooting

Figure 5-11 Severe interference in PLT Figure 5-12 Severe interference in PLT
channel(Background) channel(Normal sample)

Figure 5-13 Interference occurs because Figure 5-14 Interference occurs

gain of PLT channel is too large because gain of PLT channel is too large
(Background) (Normal sample)

Figure 5-15 Slight interference in Figure 5-16 Inadequate or no hemolysis in

WBC channel (Normal sample) WBC channel

5-12
 Troubleshooting

Figure 5-17 Slight interference in RBC Figure 5-18 Sample of too dense
channel(Normal sample) concentration in RBC channel
( Does not occur in normal situation )

Figure 5-19 Slight interference in PLT Figure 5-20 Sample of too dense
channel(Normal sample) concentration in PLT channel
(Does not occur in normal situation)

Figure 5-21 Interference in WBC Figure 5-22 Measuring interference from

channel caused by inverter inverter
Feature: sine wave with cycle of 20～26us

5-13
 Troubleshooting

Figure 5-23 Insufficient liquid in WBC bath Figure 5-24 Interference in RBC
during count channel from tubing
Feature: data length increases, the base
line of signal is not stabile.

Figure 5-25 Insufficient liquid in RBC bath Figure 5-26 Interference in PLT
during count channel from tubing
Feature: data length increases, the base
line of signal is not stabile.

Figure 5-27 Insufficient liquid in RBC Figure 5-28 Interference in WBC

bath during count channel from tubing
Feature: data length increases, the base
line of signal is not stabile.

5-14
 Troubleshooting

5.4 Common troubleshooting procedures

Background abnormal

5-15
 Troubleshooting

Clog

Diluent enough? YES

Bubbles in diluent syringe or piston

NO falls off?
YES
No

Fix this problem, clog

Change new diluent, clog solved? No
solved?
No

Yes
Yes
End
End

YES SV3 valve works properly?

Is default count time correct? No

No
Replace SV3, clog
Yes End
solved?
No Yes

Re-set the count time, clog

No
solved?
Access "Service"--"Maintenance"--"Zap
Aperture" and "Flush Aperture" and " Yes End
Clean baths", Clog solved?

Yes

End No

Is volumetric board or SV8 or SV18

Yes
broken?

Replace the defective parts, clog

No No
solved?

Connect with manufacturer

Yes

End

5-16
 Troubleshooting

HGB error

5-17
 Troubleshooting

Vacuum low

Release the the tubing

,
Does waste liquid tubing leak ? Yes Yes End
problem solved ?

No
No

Are vacuum filters blocked ? Replace new filters

,
Yes Yes End
problem solved?

No
No

Is vacuum pump leakage ? Change new pump ,

Yes
problem solved ? Yes End

No
No

Is vacuum chamber or tubing Replace the defective

Yes Yes End
Leakage ? parts , problem solved ?

No
No

Replace the defective part

,
Is SV10 broken ? Yes Yes End
problem solved?

No No

Replace this board

,
Does analog board broken ? Yes Yes End
problem solved?

No No

Connect with manufacturer

5-18
 Troubleshooting

Display error

Replace the
No anything displayed? Yes display(screen), Yes End
problem solved?

No
No

Replace LCD inverter,

Without backlight? Yes Yes End
problem solved?

No
No

Remove the J10 jumper

Only half of screen has
Yes from CPU board, Yes End
information?
problem solved?

No
No

Adjust the unique

adjustable resistor on
Contrast or backlight is bad? Yes Yes End
CPU board, problem
solved?

No
No

Replace the screen or

A continual line or some cotinual
Yes CPU board, problem Yes End
lines on the screen?
solved?

No No

Connect with the

manufacturer

5-19
 Troubleshooting

Bubbles

Diluent enough? YES

Bubbles in diluent syringe or

NO piston falls off? YES
No

Change new diluent, bubble Fix this problem, bubble

No
solved? solved?
No

Yes
Yes
End
End

YES SV3 valve works properly?

Is default count time correct? No

No
Replace SV3, clog
solved?
Yes End
No Yes

Re-set the count time,

No
bubble solved?
bubbls in sample, bubble solved? Yes End

Yes

End No

Connect with manufacturer

5-20
 Troubleshooting

Lyse out
py
Replace new solution,
Solution enough? No Yes End
problem solved?

No
Yes

Does the plastic washer on the senor Refix the washer, problem
Yes Yes End
fall off? solved?

No
No

Connect the cable well,

Does connect the sensor cable well? No Yes End
problem solved?

No
Yes

Exchange this sensor with other

Yes End
solution sensor to try, problem solved?

No

Connect with manufacturer

5-21
 Troubleshooting

48V low

Replace the the tranformer

Is transformer output AC 53V and
No and check the connector, Yes End
120V?
problem solved?

No
Yes

Does connect the transformer and Connect them well,

No Yes End
analog board well? problem solved?

No
Yes

Replace the analog board,problem

Yes End
solved?

No

Connect with manufacturer

5-22
 Troubleshooting

WBC value error

Measure more
samples(controls) and
Is WBC value correct? No Yes End
calibrate or check the 50ul
syringe, problem solved?

No
Yes

Check the Lyse and it's

Inject Lyse enough? No sysringe and valve, Yes End
problem solved?

No
Yes

Measure the general samples, the end

Adjust the WBC gain,
point of WBC diagram is between 320fl No Yes End
problem solved?
and 350fl?

No
Yes

Make sure there is not

much anitcoagulant and not
Parts differential correct? No Yes End
left the sample long time,
problem solved?

Yes No

End
Connect with manufacturer

5-23
 Troubleshooting

Analyzer fails to start

Connect AC power well,

AC input well? No Yes End
problem solved?

No
Yes

Replace fuses, problem

Fuses broken? Yes Yes End
solved?

No
No

Replace power supply

12V &5V&30V correct? No Yes End
board, problem solved?

No
Yes

Re-install system software

or replace a new
Initialization correct? No Yes End
moduleondisk, problem
solved?

Yes
No

End

Connect with manufacturer

5-24
 Troubleshooting

Background noise

When the count data of a container filled with diluent just exceeds the tolerable value of the
parameter shown in the table in ”Background Noise Check” of this chapter, the seven
possible causes of error are:

1. Dirty diluent;

2. Dirty diluent container;

3. Dirty baths;

4. Dirty valves;

5. Electronic noise affecting the counting/calculation circuit: AC line, backlight converter,

peripheral equipment such as microwave treatment machine, motors in the instrument;

6. Mechanical noise affecting the counting/calculation circuit: Vibration from motor in or

around the instrument, such as a centrifuge;

7. Tubing noise affecting the counting/calculation circuit: Liquid flow with some remained
electric charges.

z Before storing the instrument for a long time, clean and empty all fluid
tubings in the instrument with distilled water. Otherwise, the diluent salt
adheres to the baths and cannot be removed easily.

As a countermeasure to the above 7 causes, take the following actions.

Cause (1) or (2)
− Replace the diluent with diluent from a sealed container.

Cause (3) or (4)

− Clean the baths with E-Z cleanser.
− Clean the baths with probe cleanser.
− Clean valves with distilled water.
− Exchange the other same model valves to try.

Cause (5)
−Securely ground the instrument, including any optional units such as external printer.

Cause (6)
− Keep the instrument away from the vibration source.

Cause (7)
− Securely ground the tubing.

5-25

For reproducibility
This subsection describes the cases when the reproducibility for the following measuring
parameter or calculated parameter is poor.
− PLT
− HGB
− WBC
− RBC
− Data other than red cell indexes (MCV, MCH and MCHC)
− RBC and PLT coefficient related parameters
− HCT and MCV
Poor reproducibility for PLT
Possible Cause
The background data on PLT is
high
Dirty RBC aperture
Dirty RBC bath
Dirty measuring tube
Dirty probe wipe
The sample probe position is not
correct
The 3-way valve (SV11) is dirty
The 3-way valve (SV11) cannot
drain liquid empty
The diuent syringe exists bubbles
Faulty circuit
Poor reproducibility for HGB
Possible Cause
Dirty WBC measurement bath
The voltage output from the HGB
sensor is not optimal
The 3-way valve (SV12) cannot
drain liquid empty.
The 3-way valve (SV12) is dirty
Troubleshooting
Countermeasure
Refer to Background noise of this chapter
Clean the aperture.
Clean the bath. Refer to the “Maintaining Your
Analyzer” of the Operation Manual.
Clean the tube below the RBC bath.
Clean the probe wipe;
Replace a new probe wipe.
Adjust the probe position using localizer.
Clean the valves;
Replace this valve.
Replace the 3-way valve (SV11).
Remove the bubbles from the syringe.
Replace the ANALOG board.
Countermeasure
Clean the WBC bath with E-Z cleanser or probe
cleanser.
Adjust the HGB voltage. Refer to “Adjust Gain” of this
manual.
Adjust the adjustable resistance VR3 & VR4 to adjust
the output voltage of HGB to 4.4v -4.6v.
Replace the 3-way valve (SV12).
Clean or replace the valves.
5-26

The specified diluent and
hemolysing reagent were not used
Cyanide in the hemolysing reagent
has been dissolved by sunlight or
heat
The HGB 2.5mL syringe exists
bubbles
The light axis of the HGB LED is
deviated
The light of HGB is aging
The diuent syringe exists bubbles
Faulty the Analog board
Poor reproducibility for WBC
Possible Cause
The background data on WBC is
high
Dirty WBC measurement bath
Dirty measuring tube
Dirty probe wipe
The sample probe position is not
correct
The 3-way valve (SV12) cannot
drain liquid empty.
The 3-way valve (SV12) is dirty
The lyse reagent has been
dissolved by sunlight or heat
The constant current is not stable
The diuent syringe exists bubbles
Faulty the analog board
Poor reproducibility for RBC
Possible Cause
The background data on RBC is
high
Dirty RBC measurement bath
Dirty measuring tube
Troubleshooting
Use the correct reagent.
Replace the hemolysing reagent with a new reagent
and tighten the cap of the reagent bottle.
Remove the bubbles from the syringe.
Adjust the light position;
Replace the light.
Replace the HGB unit.
Remove the bubbles from the syringe.
Replace the analog board.
Countermeasure
Refer to Background Noise Check of this chapter.
Clean the WBC bath with E-Z cleanser or probe
cleanser.
Clean the tube below the WBC bath.
Clean the probe wipe;
Replace a new probe wipe.
Adjust the probe position using localizer.
Replace the 3-way valve (SV12).
Clean the valves;
Replace this valve.
Replace the lyse reagent with a new reagent and
tighten the cap of the reagent bottle.
Change the transformer or analog board.
Remove the bubbles from the syringe.
Replace the analog board.
Countermeasure
Refer to Background noise of this chapter.
Clean the RBC bath with E-Z cleanser or probe
cleanser.
Clean the tube below the RBC bath.
5-27

Dirty probe wipe
The sample probe position is not
correct
The 3-way valve (SV11) cannot
drain liquid empty.
The 3-way valve (SV11) is dirty
The constant current is not stable
The diuent syringe exists bubbles
Faulty the Analog board
Others
Error
Burn fuse when power on unit
Display is not clear
Hang during initiation
(Initiation stop)
Initiation time is too long
Keyboard, some buttons no
response
LCD screen, there is a line on
the screen
LCD screen is dark
Troubleshooting
1. Clean the probe wipe;
2. Replace a new probe wipe.
Adjust the probe position using localizer.
Replace the 3-way valve (SV11).
1. Clean the valves;
2. Replace this valve.
Change the transformer or analog board.
Remove the bubbles from the syringe.
Replace the analog board.
Possible cause(s) Recommend action
1. AC input power is not 1. Using a manostat
stable; (voltage regulator);
2. Power supply board is 2. Replace the power
short circuit. supply board.
1. Backlight is too bright or 1. Adjust the unique
too dark; resistor on the CPU board;
2. LCD screen is old. 2. Replace the LCD screen.
1. Software has some 1. Re-install the software or
problem; replace a new DOM;
2. Power supply board is 2. Replace power supply
broken. board.
Disk on module is broken. Replace hard disk (DOM)
CPU board or keyboard is Replace the CPU board or
broken. keyboard.
1. CPU board is broken if 1. Replace the CPU board;
the line is a dot line and the 2. Replace the LCD screen.
position is fixed;
2. LCD screen is faulty if
the line is a continuous
black or light line.
1. The color palette setting 1. Re-set the color palette
is not correct; to 8-color;
2. The backlight is too dark. 2. Adjust the unique
5-28
 Troubleshooting

resistor on the CPU board.

No diluent injected 1. Diluent is empty; 1. Change a new bottle of

2. The piston falls off. diluent;

2. Re-fix the piston.

No sample inspired and no SV4 valve doesn’t work. Replace the SV4 valve.
diluent injected
No initialization, but exist The CPU board is broken. Replace the CPU board.
backlight and a moment it
becomes black, the indicators
flash like saver
No backlight The inverter is broken. Replace the inverter.
Power-on is normal, but no The CPU board is broken. Replace the CPU board.
display, no initiation, no
response
WBC bath full and liquid The V12 valve doesn’t work Clean or replace the V12
overflows or dirty or doesn’t work well. valve.
WBC differential part is WBC channel gain is not Adjust WBC channel gain.
abnormal, and it’s end point correct.
at 200fL

5-29
 6 List of Spare Parts

When you order the parts or components from MINDRAY, please provide part description and
MINDRAY code, because it will help us to satisfy your requirement as soon as possible.
Using parts and components provided by MINDRAY can ensure your analyzer’s optimal
work.

6.1 Spare PCBA

Table 6-1 PCBA parts list

No. Description MINDRAY code

1 CPU board 3003-30-34910
2 Analog board 3003-30-34903
3 Drive board 3005-30-44651
4 Keypad 3003-30-34905
5 Volumetric unit 3003-30-34909
6 Switching power supply board 2800-30-28670
7 LCD adapter 3003-30-34912
8 Indicator board 3005-30-44653
9 TR60-D recorder drive board TR6D-30-16662

6-1
 List of Spare Parts

6.2 Spare Parts

Table 6-2 Spare parts list

No. Description MINDRAY code

1 Three-way solenoid valve 3003-20-34941
2 Two-way solenoid valve 3003-20-34942
3 LCD screen 0030-10-13066
4 Tubing See 6.4 Tubing for details.
5 Baths 0030-30-13194
6 Button batteryCR2032 Lithium 3V M05-100R29-02
7 FUSE Time-lag 250V 4A GLS D5*20 M07-00073F---
8 Cap assembly, waste 3006-30-74786
9 Cap assembly, lyse 3001-30-06923
10 Cap assembly, diluent 3001-30-06924
11 Cap assembly, rinse 3001-30-06925
12 DOM 0000-10-10910
13 TR60-D recorder TR6D-30-16659
14 Probe wipe assembly 3005-30-44922
15 Aspirate key 005-20-44751
16 HGB assembly 3003-30-34891
17 Sample probe assembly 3006-30-74805
18 Sample compartment assembly 3006-30-74790
19 Syringe position sensor assembly 3003-21-34924
20 RBC assembly 3003-30-34940
21 WBC assembly 3003-30-34889
22 Pump assembly 3003-30-34921
23 Rotation pump (air pump) 530B-10-05275
24 Syringe assembly 3003-30-34927
25 Vacuum assembly 3001-30-07021
26 Keypad layer 3005-20-44687
27 Screen assembly 3006-30-74788
28 Front bath clip 3001-20-07172

6-2
 List of Spare Parts

6.3 Consumables
Table 6-3 Consumbles list

No. Description MINDRAY code Packing specification

1 Air filter, GB8368-98 001-10-07054 5PCS
2 Centrifugal tube (1.5mL) A21-000013--- 20PCS
3 Tubing 1/16"X1/8",S-50-HL
AAX02002,Tygon 3001-10-07069 3.3FT
4 Tubing.3/32"X5/32",S-50-H
AAX02004,Tygon M90-100071--- 3.3FT
5 PRINT PAPER FOR AR42, no roller A30-000001--- 2PCS
6 Diluent, M-30D-5.5L A12-000042--- 5.5LX2
7 Rinse, M-30R-5.5L A12-000043--- 5.5LX2
8 Lyse, M-30L-500ML A12-000044--- 500ml
9 E-Z cleanser, M-30E A12-000045--- 100ml
10 Probe cleanser, M-30P A12-000046--- 17ml
11 Sample probe 3005-20-44868 1PCS

6-3
 List of Spare Parts

6.4 Tubing
6.4.1 Main fluidic tubing connection table
See the Table 6-4 for the connection. For the whole unit fluidic connection graph, refer to
Appendix A.

Table 6-4 Main fludic tubing connection table

No. Tube No. Start from Stop at Tube Tube length (mm) Remarks
type
T1 T40 Sample Tube 10# 450 Buffer tubing
1
probe
2 T2 T40 T91 Tube 4# 750
It connects T1
and T2. T1
3 T40 T1 T2 Tube 1# 40 must be at least
12mm deep into
T40.
4 T3 50µL SV4-NC Tube 9# 30 /
Syringe
5 T4 SV4-C SV1-NO Tube 2# 140 /
6 T5 SV3-C 10mL Tube 2# 40 /
Syringe
7 T6 SV3-NC SV1-C Tube 2# 110 /
8 T7 SV4-NO SV5-C Tube 2# 110 /
9 T8 SV5-NO T84 Tube 2# 50 /
10 T9 SV5-NC T85 Tube 2# 50 /
11 T10 SV6-C 2.5mL Tube 2# 45 /
Syringe
12 T11 SV6-NC T86 Tube 2# 40 /
13 T12 SV6-NO Connecter Tube 2# 1100 /
to lyse
container
14 T13 J12 SV17-2 Tube 1# 30 /
15 T14 J21 J12 Tube 1# 20 /
16 T15 J12 SV7-2 Tube 1# 40 /
17 T16 J18 F1 Tube 7# 20 /
18 T17 J18 F3 Tube 7# 20 /
19 T18 Vacuum Below Tube 5# 40 /
chamber WBC
metering
tube
20 T19 Vacuum Below Tube 5# 40 /

6-4
 List of Spare Parts

chamber RBC
metering
tube
21 T22 SV8-2 Middle of Tube 2# 380 /
WBC back
bath
22 T23 SV18-2 Middle of Tube 2# 370 /
RBC back
bath
23 T30 J13 SV14-2 Tube 1# 230 /
24 T31 J14 SV13-2 Tube 1# 100 /
25 T32 J5 J22 Tube 1# 650 /
26 T57 J5 SV14-1 Tube 1# 35 /
27 T58 J5 SV13-1 Tube 1# 40 /
28 T35 Beside SV15-1 Tube 1# 430 /
upper part
of RBC
isolated
chamber
29 T36 Beside SV16-1 Tube 1# 470 /
upper part
of WBC
isolated
chamber
30 T37 J4 SV15-2 Tube 1# 40 /
31 T38 J4 SV16-2 Tube 1# 40 /
32 T39 J4 SV9-NO Tube 1# 320 /
33 T41 SV9-C Pressure Tube 1# 620 /
chamber
34 T42 J18 Pressure Tube 5# 40 /
pump inlet
35 T43 Pressure Pressure Tube 5# 110 /
chamber pump
outlet
36 T44 SV9-NC Vacuum Tube 1# 250 /
chamber
37 T45 J3 SV11-2 Tube 1# 20 /
38 T46 J3 SV12-2 Tube 1# 45 /
39 T47 Vacuum SV10-2 Tube 1# 130 /
chamber
40 T48 SV10-1 J2 Tube 1# 20 /
41 T49 J2 J3 Tube 1# 25 /
42 T52 SV2-1 J1 Tube 1# 20 /

6-5
 List of Spare Parts

43 T53 J1 J2 Tube 1# 25 /
44 T54 J1 Waste Tube 5# 400 /
pump inlet
45 T55 J19 Waste Tube 5# 300 /
pump
outlet
46 T56 J20 SV3-NO Tube 1# 70 /
47 T61 Lower J27 Tube 1# 40 /
interface
of RBC
isolated
chamber
48 T62 Upper J28 Tube 1# 50 /
interface
of WBC
isolated
chamber
49 T63 Pressure Pressure Tube 1# 620 /
chamber sensor
50 T64 Vacuum Vacuum Tube 1# 420 /
chamber sensor
51 T67 J20 J17 Tube 5# 1050 /
52 T72 J21 F2 Tube 1# 20 /
53 T73 J21 F4 Tube 1# 20 /
54 T89 J27 SV11-1 Tube 1# 100 /

55 T90 J28 SV12-1 Tube 1# 110 /

The tube
length
between the
50uL
56 T91 T2 Tube 9# 15 syringe
Syringe
connecter and
T2 must be
within 3mm.

6.4.2 Assembly tubing connection table

Table 6-5 Assembly tubing connection list

No. Tube Start from Stop at Tube Tube Remarks

No. type length
(mm)
Tube Metering tube
1 T20 J8 SV8-1 50
2# assembly

6-6
 List of Spare Parts

Tube
2 T59 J8 SV7-1 60
2#
Tube
3 T21 J9 SV18-1 50
2#
Tube
4 T60 J9 SV17-1 60
2#
Tube
5 T26 J6 J14 15
1#
Tube
6 T24 J6 J23 30
1#
Tube
7 T25 J6 J24 30
1# RBC assembly
Top of the RBC Tube
8 T80 J23 15
back bath 1#
Bottom interface
Tube
9 T81 J24 of the RBC back 15
1#
bath
Tube
10 T29 J7 J13 15
1#
Tube
11 T27 J7 J25 30
1#
Tube
12 T28 J7 J26 30 WBC assembly
1#
Top of the WBC Tube
13 T82 J25 15
back bath 1#
Bottom of the Tube
14 T83 J26 15
WBC back bath 1#
Bottom of the Tube
15 T65 T33 50
RBC front bath 1#
Tube
16 T33 T87 T65 120 RBC assembly
4#
Top of RBC Tube
17 T87 T33 25
isolated chamber 1#
Below the WBC Tube
18 T66 T34 50
front bath 1#
Tube
19 T34 T88 T66 120 WBC assembly
4#
Top of WBC Tube
20 T88 T34 25
isolated chamber 1#
Lower interface of Tube Sample probe
21 T50 SV1-NC 650
the probe wipe 8# assembly
Upper interface Tube
22 T51 SV2-2 450
of the probe wipe 8#

6-7
 List of Spare Parts

Pressure Pressure Tube

23 T69 70 Negative
chamber chamber 5#
pressure
Pressure Pressure Tube
24 T70 210 chamber
chamber chamber 1#
Vacuum Tube Vacuum
25 T71 Vacuum chamber 70
chamber 7# chamber
Dispensing tube On the
Tube
26 T84 T8 above the WBC 450 shielding board
4#
bath bushing
assembly;
50mm tube to
Dispensing tube
Tube be cut is
27 T86 T11 above the WBC 330
4# included in the
bath
total tube
length.
On the
shielding board
bushing
Dispensing tube assembly;
Tube
28 T85 T9 above the RBC 390 50mm tube to
4#
bath be cut is
included in the
total tube
length.

6.4.3 Accessory tubing connection table

Table 6-6 Accessory tubing connection list

No. Tube Start from Stop at Tube Tube Remarks

No. type length
(mm)
Diluent Tube
1 T74 J17 850
container 7# Cap assembly
Inside of the Tube of diluent
2 T75 / 280
diluent container 6#
Rinse Tube Cap assembly
3 T76 J22 850
container 7# of rinse
Inside of the Tube
4 T77 / 280
rinse container 6#
Inside of the lyse Tube Cap assembly
5 T78 / 180
container 6# of lyse
Waste Tube Waste
6 T79 J19 1500
container 5# container

6-8
 List of Spare Parts

assembly

6.4.4 Tube type table

Table 6-7 tubing type list

No. Tube type Tube model MINDRAY code

1 1＃ S-50-HL M90-100071---
2 2＃ S-50-HL 3001-10-07069
3 3＃ 9023-14 M90-100031---
4 4＃ R-3603 M90-000025---
5 5＃ 2800546-100 A21-000002---
6 6＃ 2800392-100 A21-000007---
7 7＃ R-3603 M90-000024---
8 8＃ MPF tube 3.5X2 M6G-020005---
9 9＃ EVA tube M6G-020007---
10 10＃ TYGON 3350 M6G-020006---

6.4.5 Connecter table

Table 6-8 connecter table

No. Connecter table Description MINDRAY code QTY Remarks

1 J5 T420-1 M90-100028--- 1 /
2 J4、J12、J18、J21 Y420-1 M90-100028-03 4 /
3 J1、J2、J3 T430/420-1 M90-100065--- 2 /
4 J20 N230/220-1 N90-100027--- 1 /
5 J15 MTL210-1 M90-100024--- 1 /
6 J16 MLLR-06 M90-100017--- 1 Connecting the
lyse cap
7 F1、F2、F3、F4 Air filter 3001-10-07054 4 Connecting the
lyse cap
8 J22 FTLB220-1 M90-100064--- 1 On the back
cover
9 J17、J19 FTLB230-1 M90-100009--- 2 On the back
cover
10 J13、J14、J27、J28 Stainless 3001-20-06932 2 On the shielding
steel tube (φ box
3)
11 J8、J9 T420-1 M90-100028--- 2 On the volumetric
unit
12 J6、J7 T420-1 M90-100028--- 2 On the bath unit
13 J23、J24、J25、J26 L420-1 M90-100100--- 4 On the bath unit

6-9
 List of Spare Parts

6.5 Cable

Figure “No.” in the Table 6-9 is corresponding to the “No.” in Figure B-1 of Appendix B.
“MINDRAY code” in the table indicates the cable type. The quantity of No. listed in the “Figure
No.” column of the table represents the quantity of cables used.

Certain marks can be found on the cables for maintenance convenience.

Table 6-9 Cable list

MINDRAY
No. Description Connection
Code
Power cord of CPU 2800-20-28753 2800-30-28670<->3005-30-44651
1
board and drive board 2800-30-28670<->3003-30-34910
Analog board power 2800-20-28765 2800-30-28670<->3003-30-34903
2
cord
Temperature sensor 3006-20-74763 3006-20-74763<->3003-30-34910
3
connecting line
Patch cord of LCD 2800-20-28768 Screen <-> inverter
4
backlight board
5 LCD connecting line 3006-20-74764 3003-30-34912<->3003-30-34910
6 Filter power cord 2800-20-28820 Filter <->3003-30-28670
Safety earth 2800-20-28821 Filter <-> Power supply mount board
7
connection
2800-20-28822 3003-30-34910<->3003-30-34903
8 34PIN connecting line
3003-30-34910<->3005-30-44651
Inverter connecting 2800-20-28849 Inverter <->3003-30-34905
9
line
BNC head connecting BNC head<->3003-30-34899
10 3001-21-07090
line
Meniscus sensor 3006-20-74765 3003-30-34910<->3001-21-07090
11
connecting line
12 50µL motor line 3006-20-74766 50µL motor <->3005-30-44651
13 10mL motor line 3006-20-74767 10mL motor <->3005-30-44651
X motor connecting 3003-20-34936 X motor<->3005-30-44651
14
line
Y motor connecting 3003-20-34937 Y motor<->3005-30-44651
15
line
Valve/Pump control 3006-20-74768 Valve, pump <->3005-30-44651
16
line
Volumetric unit 3003-20-34896 3003-30-34909<->3003-30-34910
17
connecting line
18 Keypad and recorder 3003-20-34897 3003-30-34910<->3003-30-34905

6-10
 List of Spare Parts

connecting line 3003-30-34910<->TR6D-30-16662

19 HGB connecting line 3003-20-34898 WBC bath<->3003-30-34903
WBC bath connecting 3003-20-34938 WBC bath <->3003-30-34903
20
line
RBC bath connecting 3003-20-34939 RBC bath <->3003-30-34903
21
line
FPC connecting line 3003-30-34907 LCD<->3003-30-34912
22
of the screen
Keypad connecting 3003-30-34905<->3005-30-44653
23 3005-20-44932
line
Interface line of the
24 3006-20-74770 3005-21-44873<->3005-30-44651
solenoid drive
Detecting line of the
photocoupler photocoupler detecting tube is in the position
25 3006-21-74780
detecting tube is in <->3006-20-74777
the position
Checking line of the
26 status of the sample 3006-20-74771 Switch<->3006-20-74777
compartment door
Checking line of the
27 status of the front 3006-20-74772 Switch<->3006-20-74777
panel
Tube position
28 3006-20-74773 Switch<->3006-20-74777
checking line 1
Tube position
29 3006-20-74774 Switch<->3006-20-74777
checking line 2
10ml syringe motor photocoupler /Y motor
upper photocoupler
Motor position sensor
30 3003-21-34925 /X motor left photocoupler /X motor right
assembly
photocoupler <->3006-20-74776; through
photocoupler <->3006-20-74777
Connecting line of the 3005-30-44653<->
open button of 3005-20-44651/3003-30-34910
31 3006-20-74775
sample compartment
door
Syringe position 50µL motor photocoupler <->3006-20-74776
32 3003-21-34924
sensor assembly
33 Signal detecting line 1 3006-20-74776 3006-20-74773<->3005-30-44651
34 Signal detecting line 2 3006-20-74777 3006-20-74774<->3005-30-44651
Solenoid connecting
35 3006-21-74780 Solenoid <->3005-20-44933
line

6-11
 7 Appendices

A Fluidic Diagram

A-1
B Electric Connection Diagram

Figure B-1 Cable connection of the analyzer

B-1
 Appendices

Figure B-2 Electric connection of the analyzer

B-2
 Appendices

Figure B-3 Electric connection of the main control board

B-3
 Appendices

Figure B-4 Electric connection of the analog board

B-4
 Appendices

Figure B-5 Electric connection of the drive board

B-5
 Appendices

Figure B-6 Electric connection of the power board

B-6
 Appendices

Figure B-7 Electric connection of the volumetric board

B-7
 Appendices

Figure B-8 Electric connection of the keypad

B-8
 Appendices

Figure B-9 Electric connection of the LCD adaptor

B-9
 Appendices

Figure B-10 Electric connection of the indicator board

B-10
C Test Point of Hardware Board

C.1 CPU board test point

Test Point Mark Name Description

TP1 AVCC +12V analog Input through J1.31/33 and then supplied
input by the analog board
TP2 CLK0 Main clock 0 Frequency 45MHz; reference clock;
affecting the whole board
TP3 CLK1 Main clock 1 Frequency 45MHz, affecting the FPGA
and all devices connected to it
TP4 CLK2 Main clock 2 Frequency 45MHz, affecting the LCD
buffering
TP5 CLK3 Main clock 3 Frequency 45MHz, affecting the SDRAM
TP6 CLK4 Main clock 4 Frequency 45MHz, affecting the SDRAM
TP7 GND Digital ground
TP8 AVDD +5V analog On the condition of normal AVCC input
power supply
TP9 AGND Analog ground Same potential as the digital ground
TP10 VCC +5V digital power /
supply
TP11 VDD +3.3V digital /
power supply
TP12 GND Digital ground /
TP13 GND Digital ground /
TP14 GND Digital ground /
TP15 AOUT PWM analog Set through the software and not used
output currently
TP16 XCK LCD shift clock Frequency 9MHz, ensuring the LCD
works normally
TP17 DISCLK Crystal oscillator Frequency 45MHz, affecting the LCD and
X4 A/D conversion
TP18 PCLK LPC bridge clock Frequency 30MHz, ensuring the Super
I/O can be accessed correctly
TP19 SIOCLK Crystal oscillator Frequency 24MHz, affecting the Super I/O
X2
TP20 RTCCLK Crystal oscillator Frequency 32.768KHz, affecting the
X3 real-time clock
TP21 VDDC +1.5V digital Special power supply for the FPGA,
power supply ensuring the FPGA works normally

C-1
 Appendices

TP22 V+12 +12.8V power Not used for this board, isolated from
supply other power supply of this board and
supplied to the recorder and keypad,
affecting the recorder, buzzer and
backlight of the LCD
TP23 G+12 Power ground Ground of the +12.8 power supply
TP24 HGB HGB analog Input to the A/D of this board, marked “H”
signal on the PCB
TP25 RBC RBC analog Input to the A/D of this board, marked “R”
signal on the PCB
TP26 WBC WBC analog Input to the A/D of this board, marked “W”
signal on the PCB
TP27 PLT PLT analog signal Input to the A/D of this board, marked “P”
on the PCB
TP28 VREF A/D reference 2.5V, ensuring the A/D works normally
voltage

C-2
 Appendices

C.2 Analog test point

Test point Description Voltage Range

TP1 Output of the WBC amplifying channel 0 - 5V
Test point 1 where the HGB circuit supplies
TP2 0 - 5V
consistent current for the LED
TP3 Output of the HGB detection circuit 0 - 5V
TP4 AVCC-MON voltage monitoring point 3V ± 3%
TP5 RBC branch aperture voltage monitoring point 0 - 5V
TP6 WBC branch aperture voltage monitoring point 0 - 5V
TP7 AVSS-MON voltage monitoring point 3V ± 3%
TP8 +56VA-MON voltage monitoring point 2.2 V ± 3%
TP9 Output of the RBC amplifying channel 0 - 5V
TP10 Output of the PLT amplifying channel 0 - 5V
TP11 Output of the pressure measuring circuit 0 - 5V
TP12 Output of the vacuum measuring circuit 0 - 5V
Detection of the consistent current for the
TP13 2.5V
vacuum pressure unit
TP14 2.5V output 2.5V
TP15 AVCC power supply test point +12V
TP16 +5V power supply +5V
TP17 AVSS power supply test point -12V
TP18 +100V test point +100V
TP19 AGND 0V
Test point 2 where the HGB circuit supplies
TP20 0 - 5V
consistent current for the LED

C-3
 Appendices

C.3 Drive board test point

Test Point Mark Description

TP1 RXD_PC Test point of sending data
TP2 TXD_PC Test point of receiving data
TP3 DGND Digital ground
TP4 GND Analog ground
TP5 VCC Test power supply ＋5V
TP6 +12V ＋12V
TP7 YREF Test point of the elevating motor reference voltage
TP8 YRC Test point of the elevating motor pulse oscillatory
frequency
TP9 RST_XY Reset signal
TP10 MLREF Test point of the 10mL motor reference voltage
TP11 MLRC Test point of the 10mL motor pulse oscillatory
frequency
TP12 ULREF Test point of the 50µL motor reference voltage
TP13 ULRC Test point of the 50µL motor pulse oscillatory
frequency

C-4
D Valve Test

Name Description

V1 Together with V3 and the 10mL syringe, it supplies cleanser for the probe wipe.

Moving simultaneously with the fluidic pump, it washes and dries the outer
V2
surface of the sample probe.
It dispenses diluent aspirated by the 10mL syringe into the corresponding
V3
tubing of the analyzer by switching.
Moving simultaneously with V3 and the 10mL syringe, it aspirates sample by
V4
the sample probe or washing the inner surface of the sample probe.
Together with V3 and the 10mL syringe, it dispenses diluent into the WBC bath
V5
or RBC bath by switching.
It dispenses lyse aspirated by the 2.5mL syringe into the WBC bath by
V6
switching.
When the vacuum chamber is in the negative pressure status, it opens to drain
V7
WBC metering tube.
When the vacuum chamber is in the negative pressure status, it opens solely
V8 for WBC counting; Opening with the V14 simultaneously, it aspirates rinse to
wash the WBC back bath.
Its open connects the vacuum chamber with pressure chamber to make a
V9
positive pressure for the vacuum chamber, and this helps to flush the aperture.
Moving simultaneously with waste pump, it drains the vacuum chamber or
V10
establish vacuum in the vacuum chamber.
V11 It moves simultaneously with the waste pump to drain the RBC bath.

V12 It moves simultaneously with the waste pump to drain the WBC bath.

When the vacuum chamber is in the negative pressure status, Its open with
V13
V18 simultaneously can aspirate rinse to wash the RBC bath.
When the vacuum chamber is in the negative pressure status, Its open with V8
V14
simultaneously can aspirate rinse to wash the WBC bath.
V15 It opens to bubble-mix the RBC bath.

V16 It opens to bubble-mix the WBC bath.

When the vacuum chamber is in the negative pressure status, it opens to drain
V17
the RBC metering tube.
When the vacuum chamber is in the negative pressure status, it opens solely
V18 for RBC counting; Opening with the V13 simultaneously, it aspirates rinse to
wash the RBC back bath.

D-1
P/N: 3006-20-74778 (V1.0)