Date submitted:_____________

Biol 107L Group No. ______

Members: ___________________
Exercise 7: Nerve Physiology , ___________________
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Worksheet
Adapted Dawang & Saab-Animal Physiology Manual by Carl Ian Hallazgo and D Gualberto
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RESULTS AND OBSERVATIONS:

Table 7-A. The Synapse

Responses/Observations (*Make sure excess Ringer’s Solution have been removed)

Point of stimulation
Left gastrocnemius Right gastrocnemius

Left sciatic nerve

Right sciatic nerve

ANSWERS TO QUESTIONS:

Q7-A.1. What is a synapse?

Q7-A.2. What is the reason why both gastrocnemius muscles contracted upon stimulation of either left
or right sciatic nerve only?

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 Q7-A.3. How does Table 7 -A prove that synaptic connections and not any other connection exist between
the left and the right sciatic nerves?

Q7-A.4. Discuss the transmission of action potential along synapses.

Q7-A.5. What are the effects of some drugs and toxins in the synapse? Cite examples

EX. 7-B Propagation of Action Potential

Table 7-B Propagation of action potential

Point of stimulation : Response/Observations
Midway between the
block and the muscle

Overlapping nerve ends

on the paraffin

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Q7-B.6. What is an action potential?

Q7-B.7. What do the results in Table 7-B tell you regarding the propagation of the action potential?

Q7-B.8 Outline the series of membrane and membrane potential changes with phrases and arrows that
lead to action potential development and propagation.

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 EX. 7-C. The Neuromuscular Junction

Table 7-C.1: Effect of stimulation site

Point of stimulation Observations

Belly of Muscle A

Nerve of Muscle A

Belly of Muscle B

Nerve of Muscle B

Table 7-C.2: Effect of Acetylcholinesterase Inhibitor

Point of Observations (duration and intensity of muscle contraction)

Condition
stimulation Trial 1 Trial 2 Trial 3
muscle
Muscle in
Prostigmin nerve

muscle
Nerve in
Prostigmin nerve

Q7-C.9. Discuss your results in Table 7-B.1 and 7-B.2 in terms of conduction across the
neuromuscular junction.

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 Q7-C.10. What is the effect of prostigmin at the neuromuscular junction? Explain. Do your data reflect
the effect of prostigmin? Explain.

EX. 7-D Forms of Stimulation

Table 7-D. Effects of various stimuli

Time of OBSERVATIONS
Stimuli reaction (sec) Rank (type, intensity, frequency of muscle contraction,
etc.)

Mechanical

Thermal

Osmotic

Chemical

Electrical

Q7-D.11 From the above observation, what stimulation represents the most quantitative form of
stimulation for laboratory use? Why?

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 Q7-D.12. Define or describe the following:

a) Threshold stimulus

b) Subliminal stimulus

c) Maximal stimulus

d) Supramaximal stimulus

Q7-D.13. Discuss results in Table 7-D in terms of the "all-or-none" response in the nerve.

CONCLUSIONS:

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the written permission from Xavier University and its authors.
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