Republic of the Philippines

CAVITE STATE UNIVERSITY

Don Severino de las Alas Campus
Indang, Cavite

COLLEGE OF NURSING
DEPARTMENT OF MEDICAL TECHNOLOGY

MEDT 25: Clinical Internship 1

Skills Enhancement

COURSE LEARNING GUIDE

First Semester, AY 2022-2023

Type: Laboratory
Credit Units: 12 units

Class Schedule
BSMT 4-2-1: Monday 8:00AM-5:00PM

Course Description

This course deals with the intensive practical and theoretical training in the different sections
in the clinical laboratory of affiliating hospitals namely: Clinical Chemistry, Clinical Microscopy and
Parasitology, Microbiology, Hematology, Blood Banking, Histopathologic Technique and Cytology,
Immunology and Serology under the supervision of a licensed Medical Technologist of each section.
It provides the training for the development of Medical Technologist who possesses the knowledge,
technical skills and attitude to perform scientific laboratory investigation in aid of diagnosis, treatment
and research required in health care delivery in the country and the global community.

Course Objectives

After completing this course, the students must be able to:

1. Collect, handle and process biological specimens properly and safely

2. Perform laboratory testing accurately through the use of appropriate techniques, skills, and
technology
3. Monitor testing procedures, equipment, data and professional and technical competency
using quality assurance methodologies and laboratory management skills
4. Operate instruments properly and perform preventive and corrective maintenance. Use
computers and laboratory software competently
5. Relate research in health promotion through experimental, descriptive or
epidemiological studies.
6. Practice professionalism, work effectively with patients, peers and with multi-disciplinary and
multi-cultural teams

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 7. Manifest the following values: Honesty, Critical Thinking, Value for life; Concern for others,
Resourcefulness, Perseverance, Patience and Diligence.

Course Calendar (Skills Enhancement)

Period Course Content Course Outcome Submission

Material Based Date
Assessments
Week1
September COURSE ORIENTATION Video - -
12-17 Conference

I. Procedural and MEDT25

Rubrics Discussion Lab Module /
Week 2 Hand-outs
September a) Urinalysis - -
19-24 b) Fecalysis

II. Clinical Microscopy

c) Urinalysis 1) NSS Return Week 3

d) Fecalysis 2) Glass slide Demonstration
3) Cover slip
Week 3 4) Applicator Quiz Week 3
September stick
19-October 1 5) Sample
6) Reagent
strip
(1 bottle per
section)
III. Procedural and MEDT25
Rubrics Discussion Lab Module /
Hand-outs
a) Plated and Tubed
Culture Media
Week 4 Preparation
October 3-8 b) Gram staining - -
c) AFB staining
d) Streaking on
Various Media
(Plated, Slant, Butt,
Slant and Butt)

IV. Concept Discussion MEDT25

Lab Module /
a) Catalase Hand- - -
Week 5 b) Coagulase outs/Lecture
Notes

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October 10- c) Differentiation of Video Clip/s
15 Hemolytic Patterns

V. Bacteriology

a) Plated and Tubed 1) Agar

Culture Media powder
Preparation (nutrient/
b) Gram staining gelatin)
c) AFB staining 2) Distilled
d) Streaking on water/
Various Media Deionized
(Plated, Slant, water
Butt, Slant and 3) Spatula
Butt) 4) Stirrer Activity Week 6
5) Foil
e) Catalase 6) Alcohol
f) Coagulase 7) Lamp
g) Differentiation of 8) Measuring
Week 6 Hemolytic Patterns cylinder
October 17- 9) Weighing
22 boat
10) Conical Moving Week 6
flask Exam
11) Test tube
Rack
12) 10ml
glass tube
with screw
cap
*amount
tentative
13) Sterile
Petri dish
*amount
tentative
14) Parafilm
15) Weighing
balance/
Analytical
balancer
16) Autoclave
17) Incubator
18)Refrigerat
or
19) Frosted
Glass Slide
20) Lighter
21) 95%
Ethanol
22) Petri Dish

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 VI. Procedural and MEDT25
Week 7 Rubrics Discussion Lab Module / - -
October 24- Hand-outs/
29 a) Venipuncture:
Syringe

Week 8
October 31 – VII. Procedural and MEDT25
November 5 Rubrics Discussion Lab Module / - -
Hand-outs
a) Use of Manual
Spectrophotometer

VIII. Bacteriology

a) Plated and Tubed Same as Return Week 9

Culture Media Week 6 Demonstration
Week 9 Preparation
November b) Gram staining
7-12 c) AFB staining
d) Streaking on
Various Media
(Plated, Slant,
Butt, Slant and
Butt)

Week 10
November ACADEMIC BREAK
14-19

IX. Hematology 1.2/

Immunohematology

a) Venipuncture: 1)Phlebotomy Return Week 11

Syringe Kit Demonstration
Week 11 b) Use of Manual 2) Cuvette/
November Spectrophotometer Glass tube,
21-26 Reagents Quiz Week 11
X. Procedural and
Week 12 Rubrics Discussion MEDT25
November Lab Module /
28- a) Venipuncture: ETS Hand-outs - -
December 3 b) Capillary Puncture

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 XI. Procedural and MEDT25
Rubrics Discussion Lab Module /
Hand-outs
Week 13 a) Blood Smearing
December 5- and Staining - -
10 b) Blood Typing:
Forward and
Reverse

XII. Hematology 1.2/ 1)Phlebotomy

Immunohematology kit
2)Glass slide
a) Venipuncture: ETS 3) Reagent Return Week 14
b) Capillary Puncture 4) Glass tube Demonstration
Week 14 c) Blood Smearing and 5)Centrifuge
Dec 12-17 Staining 6) Anti sera
d) Blood Typing: Quiz Week 14
Forward and Reverse

December
19-January 1 CHRISTMAS BREAK

Week 15 XIII. Procedural and MEDT25

January 2 - 7 Rubrics Lab Module /
Discussion Hand-outs
- -
a) Venipuncture:
Winged

Week 16 XIV. Procedural and MEDT25

January 9-14 Rubrics Lab Module /
Discussion Hand-outs

a) Blood Smear: - -
WBC ID and
Count
b) Platelet ID and
Counting

XV. Hematology 1.3

a) Venipuncture: 1)Phlebotomy Return

Winged kit Demonstration Week 17
b) Blood Smear: 2) Glass slide
Week 17 WBC ID and 3) Cell Activity
January Count Counter Week 17
16-21 c) Platelet ID and 4) Differential
Counting Counter
5) Specimen
from Blood

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 smear/ or
from
Instructors
XVI. Hematology 1.3

a) Blood Smear: 1)Phlebotomy

WBC ID and kit
Count 2) Glass slide
Week 18 b) Platelet ID and 3) Cell Return
January Counting Counter Demonstration Week 18
23-28 4) Differential
Counter
5) Specimen
from Blood
smear/ or
from
Instructors

Course Materials / Readings / References

All modules, reading materials and other open educational resources will be available in the
course site (Google Classroom) on or before the scheduled session. You can access these
resources at your convenient time.

Course Requirements

For this course you need to perform 5 practical exams and 2 activities.

The grading system that will be used is:

a. Virtual Internship /Skills Enhancement

i. 50% - Virtual (EAC)
ii. 50% - Skill Enhancement
1. Attendance / Participation – 25%
2. Return Demonstration / Moving Exam/ Quiz – 25%

The passing score is 70% and that is equivalent to 3.0. Refer to the standard transmutation table
below.
Percentile
Rating Grade
96.7 – 100 1.00
93.4 - 96.6 1.25
90.1 - 93.3 1.50
86.7 - 90.1 1.75

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 83.4 - 86.6 2.00
80.1 - 83.3 2.25
76.7 – 80.0 2.50
73.4 - 76.6 2.75
70.00 - 73.4 3.00
50.00 - 69.9 4.00
Below 50 5.00
INC Passed the course but lack some requirements
Dropped If unexcused absence is at least 20% of the Total Class Hours
Total Class Hours/Semester: (12 units=832 hrs)

Course Policies

1. Your clinical instructor will ask you to download and read the attached learning guide for
reference. The downloaded learning guide will serve as the course work plan for the entire 18
weeks. Once you received and fully understand the attached file, you are required to
Comment/Write your FULL NAME (Surname, First Name, MI), your student number and
DATE when the file was downloaded as an acknowledgement on the platform the file was
uploaded.

2. At the start of the semester, you will be asked to sign an Honor Pledge as an oath to your
honest commitment and participation in the flexible learning arrangement to be employed by
the University this Semester.

3. This course will employ Flexible learning. Majority will be conducted in face to face set up,
and the rest will be virtual.

4. Most virtual classes time will be used to assist the instructors in Virtual internship for the
monitoring and checking of the students output, This time will also be given to accomplish
some of the virtual internship activities.

5. During Face-to-face sessions, the students shall wear proper attire (White uniform and Black
shoes)

6. During Virtual sessions, students are expected to wear decent attire

7. The primary online platform to be used is Google Classroom. If possible, secure a CvSU
email address; nonetheless, any valid Google Mail account may be allowed. Facebook
Messenger will be utilized for sending urgent announcements. Synchronous classes will make
use of Zoom, Google Meet, and other video conferencing platforms.

8. As a student at Cavite State University, you are expected to uphold the university tenets of
truth, excellence and service. Thus, you must practice academic integrity through the entire
duration of the course, which will be attested by your honesty pledge to be submitted in the
first week of classes and before major examinations.

9. In connection with no. 9, cheating during assignments, activities, quizzes, and exams are
strictly prohibited. Making copies of any questions from the said activities in any form are also

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 forbidden. Dissemination of any materials received for this course to anyone not enrolled in
this course is likewise NOT allowed. Any such circumstances shall be dealt with accordingly
by the instructors and the administrators.

10. The University’s Grading System shall be followed as appropriate for this flexible learning
arrangement.

11. Majority of the activities that will be held face to face will be graded as return demonstration,
since it is already part of the students’ previous subjects

12. The faculty will implement strict guideline for waste segregation that will be further discuss on
the student’s orientation (Punishment for those who will disobey must be expected)

Assessment Guides
The rubric assessment will be given ahead of time to give the students ample time to
prepare and review for the given activity/return demonstration.

Information about the Clinical Instructor

Andrea Cassandra P. Llantos, RMT

Instructor, MEDT 25 Laboratory
llantosandrea@gmail.com
FB/Messenger: Andrea Llantos

References and Supplementary Readings

Abbas, Abul K. and Andrew H. Lichtman. Basic Immunology: Functions and Disorders of the
Immune System 3rd ed. Philadelphia: W.B. Saunders, 2008.
Alcamo, Edward I. Fundamentals of Microbiology 6th ed. Boston: Jones & Bartlett Publishers,
2001.
.Anderson S. and K. Poulsen. Anderson’s Atlas of Hematology. USA: Lippincott Williams and
Wilkins, 2003. Anderson, Shauna and
Susan Cockyane. Clinical Chemistry: Concepts and Applications. USA: Waveland Press Inc.,
2007.
Arneson, W. and J. Brickell. Clinical Chemistry: A Laboratory Perspective. USA: F.A. Davis Co.,
2007.
Bishop, Michael L. et.al. Clinical Chemistry: Principles, Procedures, Correlation’s, 5th ed.
Philadelphia: Lippincott Williams,
Philadelphia, 2005.
Brooks, Geoff. Jawetz, Melnick & Adelberg’s Medical Microbiology 24th ed. Boston: Mc Graw
Hill, 2007.

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Brown, Barbara. Hematology: Principles and Procedures 5th ed. Philadelphia: Lea and Febiger,
1993.
Department of Health (DOH). Manual of Standards for Blood Banks and Blood Centers in the
Philippines. Manila: DOH, 1996.
Dimmock, Nigel, Andrew Easton and Keith Leppard. Introduction to Modern Virology 6th ed.
USA: Wiley-Blackwell, 2007.
Funke, Berdell. Microbiology: An Introduction. USA: Addison Wesley Publishing Co., 2007.
Garcia, Lynn Shore. Diagnostic Medical Parasitology 5th ed. USA: ASM Press, 2006.
Gardner, David and Dolores Shoback. Greenspan’s Basic and Clinical Endocrinology 8th ed.
McGraw-Hill Professional, 2007.
Garza, Diana and Kathleen Becan-McBride. Phlebotomy Handbook: Blood Collection Essentials
7th ed. USA: Prentice Hall, 2004.
Gladwin, Mark and Bill Trattler. Clinical Microbiology Made Ridiculously Simple 4th ed. USA:
MedMaster, Inc., 2007.
Greer J., J. Foerster and J. Lukens. Wintrobe’s Clinical Hematology 11th ed. USA: Lippincott
Williams and Wilkins, 2003.
Gregorios-Bruce, Jocelyn. Histopathologic Techniques 2nd ed. Quezon City: JMC Press, 2006.
Hibbert, Brynn. Quality Assurance in the Analytical Chemistry Laboratory. USA: Oxford
University Press, 2007.
Hoeltke, Lynn. Phlebotomy: Principles and Procedures. USA: Delmar Learning, 2006.
Hoffman R. et.al. Hematology: Basic Principles and Practice 4th ed. USA: Churchill Livingstone,
2004.
Ludwig, Jurgen. Handbook of Autopsy Practice 3rd ed. USA: Humana Press, 2002.
Mahon, Connie. Clinical Laboratory Immunology. USA: Prentice Hall, 2005.
Marques M. Quick Guide to Coagulation Testing. USA: AACC Press, 2006.
Rudmann, Sally. Textbook of Blood Banking and Transfusion. USA: Elsevier, 2005.
Stanley, J. Essentials of Immunology and Serology. USA: Delmar Learning, 2002.
Steininger, Cheryl et al. Clinical Hematology: Principles, Procedures and Correlations.
Philadelphia: J.B. Lippincot, 1992.
Stevens, C.D. Clinical Immunology and Serology: A Laboratory Perspective 2nd ed. USA: F.A.
Davis Co., 2003.
Strasinger S.K. and Di Lorenzo M.S, Urinalysis and Body Fluids 5th ed. USA: F.A. Davis
Company, 2008.
Vandepitte J., J. Verhaegen and K. Engbaek. Basic Laboratory Procedures in Clinical
Bacteriology 2nd ed. World Health Organization,
2003.
Varnadoe, Lionel A., Medical Laboratory Management and Supervision: Operations, Review
and Study Guide, Philadelphia: Elsevier
A. Inc., 1996.